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The map label for this gene is purA [H]

Identifier: 18976680

GI number: 18976680

Start: 324065

End: 325084

Strand: Direct

Name: purA [H]

Synonym: PF0308

Alternate gene names: 18976680

Gene position: 324065-325084 (Clockwise)

Preceding gene: 18976675

Following gene: 18976688

Centisome position: 16.98

GC content: 47.25

Gene sequence:

>1020_bases
ATGCCAAGTGTAATTGTTGTTGGTGGACAGTGGGGAGATGAGGGAAAGGGTTCAATAATAGCATATCTCGCACTCCACGA
CGAGCCAGAGATCATAGCAAGAGGTGGCGTTGGAACAAATGCTGGGCACAGCGTGGTTATAAACGGGAAGAAGTACGCTG
TGAGGCAGTTGCCCACTGGGTTTATGCAGACCAAGGCTAGGCTTCTTGTAGGGGCTGGAGTTTTAGTTGATCCTGAGGTC
TTCTTTTATGAGCTCGAGCACTTAAAGGATTTCAATGTTGCTGAGAGGGTTGGAATAGACTACAGGTGTGCAATAATCGA
GCCAAAGCACAAGGAGATGGACAGAAAAAATGACTATCTTCACGGTACCATAGGAACAACTGGAAGTGGATGTGGACCTG
CCAATGCTGATAGGGTCATGAGGGTGGCAAAGCAGGCAAAAGACATTAAAGAGTTGGAGCCTTATCTTACGGATGTTGCT
GCAGAGGTTAACGATGCCCTGGATGAGGGGGCACTCGTCTTAGTTGAGGGAACCCAGGGGTTTGGCTTAAGCCTTTATTA
CGGTACTTATCCCTACGTTACCTCAAAAGACACCACAGCCTCTGCAGTTGCGAGTGACGTTGGAATAGGGCCTACGAGGG
TAGATGATGTGATAGTGGTCTTCAAGAGTTTCCCAACGAGGGTAGGTGCTGGTCCATTCCCCACGGAGATGCCAATGGAA
GAAGCAGATAGGCTTGGATTGATCGAGTATGGAACAGTTACTGGAAGGAGGAGAAGGGTAGGATGGTTTGACTTTGAGAT
GGCTAGATACGCTGCTAGAATTAACGGTGCCACAATGCTCGCTGTTACAATGCTGGATAAGTACGATAAGGAAGCTTTTG
GAATTACTGATTACGATAAGCTTCCCAGGAAGGCAAAGGAGTTTATTGAGGAGATTGAAGAGAGAGTTGGAGTTCCAGTA
GGTTTAATAAAGACGGGTCCAGAACTGGAGCACATTATAGATTTGAGGGAGAACATCTAG

Upstream 100 bases:

>100_bases
TTTAGTTATAAATGATTGGTGGTCATCATTTATTTGACGTAAAAATGTTTATTCCGTAAGTGTTTTAAGGGGGTTATTGA
CTTAGCTTTGGGTAAGAGCG

Downstream 100 bases:

>100_bases
CTTTTCTTTTTATTTGGTTTATCCTTTTGTTTTAAGTTTTAAGAAAGAATAGTAAACTTCTGGTGCCCCGGCCGGGATTT
GAACCCGGGGCGCGGGCTCG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MPSVIVVGGQWGDEGKGSIIAYLALHDEPEIIARGGVGTNAGHSVVINGKKYAVRQLPTGFMQTKARLLVGAGVLVDPEV
FFYELEHLKDFNVAERVGIDYRCAIIEPKHKEMDRKNDYLHGTIGTTGSGCGPANADRVMRVAKQAKDIKELEPYLTDVA
AEVNDALDEGALVLVEGTQGFGLSLYYGTYPYVTSKDTTASAVASDVGIGPTRVDDVIVVFKSFPTRVGAGPFPTEMPME
EADRLGLIEYGTVTGRRRRVGWFDFEMARYAARINGATMLAVTMLDKYDKEAFGITDYDKLPRKAKEFIEEIEERVGVPV
GLIKTGPELEHIIDLRENI

Sequences:

>Translated_339_residues
MPSVIVVGGQWGDEGKGSIIAYLALHDEPEIIARGGVGTNAGHSVVINGKKYAVRQLPTGFMQTKARLLVGAGVLVDPEV
FFYELEHLKDFNVAERVGIDYRCAIIEPKHKEMDRKNDYLHGTIGTTGSGCGPANADRVMRVAKQAKDIKELEPYLTDVA
AEVNDALDEGALVLVEGTQGFGLSLYYGTYPYVTSKDTTASAVASDVGIGPTRVDDVIVVFKSFPTRVGAGPFPTEMPME
EADRLGLIEYGTVTGRRRRVGWFDFEMARYAARINGATMLAVTMLDKYDKEAFGITDYDKLPRKAKEFIEEIEERVGVPV
GLIKTGPELEHIIDLRENI
>Mature_338_residues
PSVIVVGGQWGDEGKGSIIAYLALHDEPEIIARGGVGTNAGHSVVINGKKYAVRQLPTGFMQTKARLLVGAGVLVDPEVF
FYELEHLKDFNVAERVGIDYRCAIIEPKHKEMDRKNDYLHGTIGTTGSGCGPANADRVMRVAKQAKDIKELEPYLTDVAA
EVNDALDEGALVLVEGTQGFGLSLYYGTYPYVTSKDTTASAVASDVGIGPTRVDDVIVVFKSFPTRVGAGPFPTEMPMEE
ADRLGLIEYGTVTGRRRRVGWFDFEMARYAARINGATMLAVTMLDKYDKEAFGITDYDKLPRKAKEFIEEIEERVGVPVG
LIKTGPELEHIIDLRENI

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=29.81220657277, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=27.906976744186, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI40316944, Length=393, Percent_Identity=28.498727735369, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=32.5526932084309, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25146553, Length=421, Percent_Identity=31.1163895486936, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI25146555, Length=421, Percent_Identity=31.1163895486936, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=30.4449648711944, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI21356033, Length=427, Percent_Identity=27.6346604215457, Blast_Score=164, Evalue=9e-41,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 37136; Mature: 37005

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVIVVGGQWGDEGKGSIIAYLALHDEPEIIARGGVGTNAGHSVVINGKKYAVRQLPTG
CCCEEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEECCCEEEHHHCCCH
FMQTKARLLVGAGVLVDPEVFFYELEHLKDFNVAERVGIDYRCAIIEPKHKEMDRKNDYL
HHHHHHHHHEECCEEECCHHHHHHHHHCCCCCHHHHCCCCEEEEEECCHHHHHCCCCCEE
HGTIGTTGSGCGPANADRVMRVAKQAKDIKELEPYLTDVAAEVNDALDEGALVLVEGTQG
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
FGLSLYYGTYPYVTSKDTTASAVASDVGIGPTRVDDVIVVFKSFPTRVGAGPFPTEMPME
CCEEEEECCCCEEECCCCHHHHHHHHCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCHH
EADRLGLIEYGTVTGRRRRVGWFDFEMARYAARINGATMLAVTMLDKYDKEAFGITDYDK
HHHCCCEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCHHH
LPRKAKEFIEEIEERVGVPVGLIKTGPELEHIIDLRENI
HHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PSVIVVGGQWGDEGKGSIIAYLALHDEPEIIARGGVGTNAGHSVVINGKKYAVRQLPTG
CCEEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEECCCEEEHHHCCCH
FMQTKARLLVGAGVLVDPEVFFYELEHLKDFNVAERVGIDYRCAIIEPKHKEMDRKNDYL
HHHHHHHHHEECCEEECCHHHHHHHHHCCCCCHHHHCCCCEEEEEECCHHHHHCCCCCEE
HGTIGTTGSGCGPANADRVMRVAKQAKDIKELEPYLTDVAAEVNDALDEGALVLVEGTQG
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
FGLSLYYGTYPYVTSKDTTASAVASDVGIGPTRVDDVIVVFKSFPTRVGAGPFPTEMPME
CCEEEEECCCCEEECCCCHHHHHHHHCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCHH
EADRLGLIEYGTVTGRRRRVGWFDFEMARYAARINGATMLAVTMLDKYDKEAFGITDYDK
HHHCCCEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCHHH
LPRKAKEFIEEIEERVGVPVGLIKTGPELEHIIDLRENI
HHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]