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The map label for this gene is lysX [H]

Identifier: 18976581

GI number: 18976581

Start: 225439

End: 226260

Strand: Direct

Name: lysX [H]

Synonym: PF0209

Alternate gene names: 18976581

Gene position: 225439-226260 (Clockwise)

Preceding gene: 18976580

Following gene: 18976583

Centisome position: 11.81

GC content: 39.42

Gene sequence:

>822_bases
ATGAAAATAGGGATAACGTTTACAATCCTTAGAAAAGAGGAGCTTATGTTTAAGAATAAGGCTAAAGAATTTGGGGAAGT
TGTAATGATAAATGATAGAGAAGTTGTCTTCCCAGGTAGATACGATGTTGATGTTGTTATAATAAGAGATATAAGCCATT
TCAAAGCTCTCTATATTTCAAAGTTATTTGAAGAAGCAGGTGTTCCAGTTGTTAATCCGCACTGGGTAATTTATGAGGCT
GGAGATAAGTTGCTGGCAACCTTAAGGTTGGAAAAGAAGGTTCCAGTTCCCAGGTGGGCCGTGGCTTTTGATAAAGACTC
CGCAATAAGGGCAACAAAAGAGCTTGGATATCCTGTTGTTGTTAAACCAGTCTTTGGTAGCTGGGGAAGATTAGTGGCGA
AAATTCAAGATGAATTCTCTGCAGAAGGAATATTTGAGCATAGGGAGTGGATGGGGAATCCTCTCAACAAGATATACTAC
ATCCAGAAATATGTCGAAAAACCTGGAAGGGACATTAGAGCAATTGTAATAGGAGGAGAGTTTATAACCGCTATATACAG
GTATTCTGATCACTGGATTACAAATGCTGCAAGAGGAGCAAAGGCAGAACCCTGTAATGATGAAGAAGTTATGGATATAG
CAGTTAAAGCCTGGGAAGCTTTTGGTGAAGGGGCTTTGGCGATAGACATATTTGAAAGTCCTGAGGGATTGTTGGTGAAT
GAAGTTAACCCAATCATGGAATTTAAGCTAGCTTACGAAACGACGAATGCTGATATTCCTGGGAAGTTAGTTGAGTATGC
AGTTAACTATGCAAAGAGTTAG

Upstream 100 bases:

>100_bases
GTTGTTGGTTCGCCGAATCCTGAAGATGTTCTTGAATTCTTAGAAAAAGCGAAAAAGAACGTTGAAAAAGATGAGAAAAA
GCTTGAGGAGTTGAGGCAGA

Downstream 100 bases:

>100_bases
TATGCCTCGGCAAGTATTGGCCTTGTTGTCCATTTGTGCTCCTCTACTTCAATTCTAATGTCGAGAGGCTCGAGCTTTTT
AACTTCGAATCCTCTCTTTT

Product: ribosomal protein s6 modification protein

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKIGITFTILRKEELMFKNKAKEFGEVVMINDREVVFPGRYDVDVVIIRDISHFKALYISKLFEEAGVPVVNPHWVIYEA
GDKLLATLRLEKKVPVPRWAVAFDKDSAIRATKELGYPVVVKPVFGSWGRLVAKIQDEFSAEGIFEHREWMGNPLNKIYY
IQKYVEKPGRDIRAIVIGGEFITAIYRYSDHWITNAARGAKAEPCNDEEVMDIAVKAWEAFGEGALAIDIFESPEGLLVN
EVNPIMEFKLAYETTNADIPGKLVEYAVNYAKS

Sequences:

>Translated_273_residues
MKIGITFTILRKEELMFKNKAKEFGEVVMINDREVVFPGRYDVDVVIIRDISHFKALYISKLFEEAGVPVVNPHWVIYEA
GDKLLATLRLEKKVPVPRWAVAFDKDSAIRATKELGYPVVVKPVFGSWGRLVAKIQDEFSAEGIFEHREWMGNPLNKIYY
IQKYVEKPGRDIRAIVIGGEFITAIYRYSDHWITNAARGAKAEPCNDEEVMDIAVKAWEAFGEGALAIDIFESPEGLLVN
EVNPIMEFKLAYETTNADIPGKLVEYAVNYAKS
>Mature_273_residues
MKIGITFTILRKEELMFKNKAKEFGEVVMINDREVVFPGRYDVDVVIIRDISHFKALYISKLFEEAGVPVVNPHWVIYEA
GDKLLATLRLEKKVPVPRWAVAFDKDSAIRATKELGYPVVVKPVFGSWGRLVAKIQDEFSAEGIFEHREWMGNPLNKIYY
IQKYVEKPGRDIRAIVIGGEFITAIYRYSDHWITNAARGAKAEPCNDEEVMDIAVKAWEAFGEGALAIDIFESPEGLLVN
EVNPIMEFKLAYETTNADIPGKLVEYAVNYAKS

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of 'Glu-54' of lysW [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=223, Percent_Identity=29.1479820627803, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31011; Mature: 31011

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGITFTILRKEELMFKNKAKEFGEVVMINDREVVFPGRYDVDVVIIRDISHFKALYIS
CEEEEEEEEEEHHHHHHHHHHHHHCCEEEECCCEEEECCCCCEEEEEEECHHHHHHHHHH
KLFEEAGVPVVNPHWVIYEAGDKLLATLRLEKKVPVPRWAVAFDKDSAIRATKELGYPVV
HHHHHCCCCEECCCEEEEECCCEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHCCCCEE
VKPVFGSWGRLVAKIQDEFSAEGIFEHREWMGNPLNKIYYIQKYVEKPGRDIRAIVIGGE
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCH
FITAIYRYSDHWITNAARGAKAEPCNDEEVMDIAVKAWEAFGEGALAIDIFESPEGLLVN
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEE
EVNPIMEFKLAYETTNADIPGKLVEYAVNYAKS
CCCCHHEEEEEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKIGITFTILRKEELMFKNKAKEFGEVVMINDREVVFPGRYDVDVVIIRDISHFKALYIS
CEEEEEEEEEEHHHHHHHHHHHHHCCEEEECCCEEEECCCCCEEEEEEECHHHHHHHHHH
KLFEEAGVPVVNPHWVIYEAGDKLLATLRLEKKVPVPRWAVAFDKDSAIRATKELGYPVV
HHHHHCCCCEECCCEEEEECCCEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHCCCCEE
VKPVFGSWGRLVAKIQDEFSAEGIFEHREWMGNPLNKIYYIQKYVEKPGRDIRAIVIGGE
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCH
FITAIYRYSDHWITNAARGAKAEPCNDEEVMDIAVKAWEAFGEGALAIDIFESPEGLLVN
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEE
EVNPIMEFKLAYETTNADIPGKLVEYAVNYAKS
CCCCHHEEEEEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12963379 [H]