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The map label for this gene is alaXL [H]

Identifier: 18976557

GI number: 18976557

Start: 195786

End: 197000

Strand: Direct

Name: alaXL [H]

Synonym: PF0185

Alternate gene names: 18976557

Gene position: 195786-197000 (Clockwise)

Preceding gene: 18976556

Following gene: 18976562

Centisome position: 10.26

GC content: 37.04

Gene sequence:

>1215_bases
ATGGAGAGGTTATACTATATTGATCCCTATCTAAAGAAGGCAACCGCTAAAATAGTTGATTTAAAATTTGAGGGAGATAA
CGTTAAAGTCGAGTTGGACAGGACTATATTTTATCCAGAAGGGGGCGGACAACCTGGGGATAAAGGAGTTATCAAAGGCG
AGGGTTTTGAAATAATAGTAGAAAATACTATCGAAAAAGAGGGAAGAATATTTCACATTGGAAAGCTAATTGGAAAAACT
CCTAATAAAGGGGATAGTGTTGAGCTTGAGCTGGATTGGGACTGGAGATATGGAAATATGCAAATTCATACAGGCCAGCA
CATTCTTTCTGCTGTTTTGAAGAAAATGTATAATTTAGATACAACTGGTTTTACAATCTTCGGTGAATATGCTAAAATTG
AAGTAAATGGGGAGATTACTTGGGACATAATTGAAAGAGCCGAGTTAGAGGCAAATAAGATTATCATTAGTGATTTGCCA
GTAATAATTGAGGAATATCACGAACTTCCTGAGGAATTGGCTTCAGAGCTTAGAAAAAGTGTGGCTAAGGTAAAAGGAAA
AATTAGGATTGTTAAGATTGGTGATGTTGACATAACACCATGTGGAGGAACTCACGTTAGATCAACTAAGGAAGTTGGTA
TAATAAAAGTTCTCAGGTTCTACAAGAAATCGAGAGATCTTTGGAGGATAGAGTTCACATGTGGATTTAGGGCTATAAGG
GAGATTAATATAATTCTGCAAGATTACTTGAATTCTTTGGAGCTAATGTCAAATAAAAATCCTCCCCTGGTTAAGCGGGT
TAAGGAATTATTGGACACAACTAACTCCCTTGAAGAAAAGATAGAAGAGTTAAGAAGAGAGATTTGGGAATGGAAAGGCA
AAGCGCTCATAGAAAAGGCTACTAAAATTGGGGATTATCTTGTAGTTACTTATGTTGAATCTTGGGACATGAAAGATGCC
CAAGCATTTGCGGTGGATTTTGTGAAGAGAAATCCGAAAACGATACTACTGTTAGCAAGCGAAAAATATGTGGTCTTTGC
TAGGAATGAAAATGTTCCAGTCTCAATGAGGGAATTGTTCAGAAAAGTTGCAAGTGAACTTGGAGGAAAAGGAGGGGGAA
CCGATAATTTGGCTAGGGGTAAAGTAGATGCTAAACCTGAGACTATTTTGAGTGTTGCCTTAGAAAAGGTTAAAGAGACC
TTACAATTAGATTAA

Upstream 100 bases:

>100_bases
ATGGAGAGAGAAAACTTCTTCAGGCTCAAGAGAATTAAGGCATTGCTTGAGGCTAGAGAATCAATGTAATTTTTTTTATT
TCGGGTTTGGGTGAAGTCTT

Downstream 100 bases:

>100_bases
GCTTTTTCTCAACTAATTCCCATTGCCACTCTGGAACCTTTGGTTTTTCTACTTTTTTCAGAGCTTTTTCTCTGTCCAAT
AGTCCGTAACGCACTAGAGC

Product: partial alanyl-tRNA synthetase matches COOH terminus half

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MERLYYIDPYLKKATAKIVDLKFEGDNVKVELDRTIFYPEGGGQPGDKGVIKGEGFEIIVENTIEKEGRIFHIGKLIGKT
PNKGDSVELELDWDWRYGNMQIHTGQHILSAVLKKMYNLDTTGFTIFGEYAKIEVNGEITWDIIERAELEANKIIISDLP
VIIEEYHELPEELASELRKSVAKVKGKIRIVKIGDVDITPCGGTHVRSTKEVGIIKVLRFYKKSRDLWRIEFTCGFRAIR
EINIILQDYLNSLELMSNKNPPLVKRVKELLDTTNSLEEKIEELRREIWEWKGKALIEKATKIGDYLVVTYVESWDMKDA
QAFAVDFVKRNPKTILLLASEKYVVFARNENVPVSMRELFRKVASELGGKGGGTDNLARGKVDAKPETILSVALEKVKET
LQLD

Sequences:

>Translated_404_residues
MERLYYIDPYLKKATAKIVDLKFEGDNVKVELDRTIFYPEGGGQPGDKGVIKGEGFEIIVENTIEKEGRIFHIGKLIGKT
PNKGDSVELELDWDWRYGNMQIHTGQHILSAVLKKMYNLDTTGFTIFGEYAKIEVNGEITWDIIERAELEANKIIISDLP
VIIEEYHELPEELASELRKSVAKVKGKIRIVKIGDVDITPCGGTHVRSTKEVGIIKVLRFYKKSRDLWRIEFTCGFRAIR
EINIILQDYLNSLELMSNKNPPLVKRVKELLDTTNSLEEKIEELRREIWEWKGKALIEKATKIGDYLVVTYVESWDMKDA
QAFAVDFVKRNPKTILLLASEKYVVFARNENVPVSMRELFRKVASELGGKGGGTDNLARGKVDAKPETILSVALEKVKET
LQLD
>Mature_404_residues
MERLYYIDPYLKKATAKIVDLKFEGDNVKVELDRTIFYPEGGGQPGDKGVIKGEGFEIIVENTIEKEGRIFHIGKLIGKT
PNKGDSVELELDWDWRYGNMQIHTGQHILSAVLKKMYNLDTTGFTIFGEYAKIEVNGEITWDIIERAELEANKIIISDLP
VIIEEYHELPEELASELRKSVAKVKGKIRIVKIGDVDITPCGGTHVRSTKEVGIIKVLRFYKKSRDLWRIEFTCGFRAIR
EINIILQDYLNSLELMSNKNPPLVKRVKELLDTTNSLEEKIEELRREIWEWKGKALIEKATKIGDYLVVTYVESWDMKDA
QAFAVDFVKRNPKTILLLASEKYVVFARNENVPVSMRELFRKVASELGGKGGGTDNLARGKVDAKPETILSVALEKVKET
LQLD

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=375, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI217416402, Length=375, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789048, Length=393, Percent_Identity=25.9541984732824, Blast_Score=117, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6324288, Length=286, Percent_Identity=32.1678321678322, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324911, Length=234, Percent_Identity=27.3504273504274, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI18543305, Length=417, Percent_Identity=23.5011990407674, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582809, Length=467, Percent_Identity=22.6980728051392, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45552267, Length=467, Percent_Identity=22.6980728051392, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 46064; Mature: 46064

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLYYIDPYLKKATAKIVDLKFEGDNVKVELDRTIFYPEGGGQPGDKGVIKGEGFEIIV
CCEEEEECHHHHHCCEEEEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCEECCCCEEEE
ENTIEKEGRIFHIGKLIGKTPNKGDSVELELDWDWRYGNMQIHTGQHILSAVLKKMYNLD
ECCCCCCCCEEEEEHHHCCCCCCCCEEEEEEEEEEEECCEEEECHHHHHHHHHHHHHCCC
TTGFTIFGEYAKIEVNGEITWDIIERAELEANKIIISDLPVIIEEYHELPEELASELRKS
CCCEEEEEEEEEEEECCEEEEEEHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
VAKVKGKIRIVKIGDVDITPCGGTHVRSTKEVGIIKVLRFYKKSRDLWRIEFTCGFRAIR
HHHHCCCEEEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHH
EINIILQDYLNSLELMSNKNPPLVKRVKELLDTTNSLEEKIEELRREIWEWKGKALIEKA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHH
TKIGDYLVVTYVESWDMKDAQAFAVDFVKRNPKTILLLASEKYVVFARNENVPVSMRELF
HHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHH
RKVASELGGKGGGTDNLARGKVDAKPETILSVALEKVKETLQLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERLYYIDPYLKKATAKIVDLKFEGDNVKVELDRTIFYPEGGGQPGDKGVIKGEGFEIIV
CCEEEEECHHHHHCCEEEEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCEECCCCEEEE
ENTIEKEGRIFHIGKLIGKTPNKGDSVELELDWDWRYGNMQIHTGQHILSAVLKKMYNLD
ECCCCCCCCEEEEEHHHCCCCCCCCEEEEEEEEEEEECCEEEECHHHHHHHHHHHHHCCC
TTGFTIFGEYAKIEVNGEITWDIIERAELEANKIIISDLPVIIEEYHELPEELASELRKS
CCCEEEEEEEEEEEECCEEEEEEHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
VAKVKGKIRIVKIGDVDITPCGGTHVRSTKEVGIIKVLRFYKKSRDLWRIEFTCGFRAIR
HHHHCCCEEEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHH
EINIILQDYLNSLELMSNKNPPLVKRVKELLDTTNSLEEKIEELRREIWEWKGKALIEKA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHH
TKIGDYLVVTYVESWDMKDAQAFAVDFVKRNPKTILLLASEKYVVFARNENVPVSMRELF
HHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHH
RKVASELGGKGGGTDNLARGKVDAKPETILSVALEKVKETLQLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]