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The map label for this gene is ybiK [C]

Identifier: 18976514

GI number: 18976514

Start: 151648

End: 152568

Strand: Direct

Name: ybiK [C]

Synonym: PF0142

Alternate gene names: 18976514

Gene position: 151648-152568 (Clockwise)

Preceding gene: 18976513

Following gene: 18976515

Centisome position: 7.95

GC content: 43.65

Gene sequence:

>921_bases
ATGGTAGCTATTATTGTCCATGGCGGAGCGGGAACAATTAGAAAAGAGGAGAGGATTCCAAAGATCATCGAGGGAGTTAG
GGAGGCAGTTTTAACCGGTTGGAGAGAACTGAAAAAGGGTTCTGCCCTTGATGCTGTTGAAGAAGCCGTAAAAGTTCTTG
AGGACAATCCTCTCTTTAATGCTGGTACAGGAAGCGTTTTGACCTTAGATGGAAAAGTAGAGATGGACGCTGCAATAATG
AGAGGAAAAACATTGGATGCTGGTGCTGTTGCAGGGATATGGGGAGTGAAAAACCCAATTAGTGTGGCAAGAAAAGTTAT
GGAAAAGACTGATCATGTATTACTTATTGGAGAAGGAGCTGTTAAATTTGCAAGGCTAATGGGTTTTCCTGAATACGATC
CCACAACAGAAGAGAGAAGAAAACAGTGGGAGGAGTTGAGGAAAAAACTACTTGAAACTGGAGAAATACGCCACTGGAAA
AAGTTAAGTGAGCTTATAAAGGAATATCCAGAAGTCTTAAGGAGTACCGTAGGAGCTGTAGCCTTTGATGGGGAGGAGAT
AGTAGCTGGAACTTCAACTGGGGGAGTATTCCTTAAGATGTTTGGTAGGGTAGGGGATACTCCTATAATCGGGGCTGGAA
CGTATGCAAATGAGGTTGCTGGGGCTTCATGTACTGGTTTGGGGGAGGTTGCAATTAAGCTAAGCTTAGCGAAAACAGCA
ACTGACTTCGTAAGGCTCGGTTTGGATGCTCAAGCTGCAAGTGAAGCAGCAATAAGATTAGCTACAAAGTACTTTGGGCC
TGATACAATGGGAATAATCATGGTTGACTCTAATGGGAATGTTGGCTTTGCTAAGAATACAAAACACATGAGTTACGCAT
TTATGAAGGAAGGCATGAAGGAACCGGAGGCAGGGGTGTAA

Upstream 100 bases:

>100_bases
TACCTCGAAAACATAGGAGAGACTATAATCAATATGGCGATAAGTTAACGTTTCCATAACTTTTTTAACCTTTCAATTTT
GTTAAATACGGGGAGGATGC

Downstream 100 bases:

>100_bases
TGCTTAAAAGTCTTTGGTATCTAAACTTTTCCACACTATTCTTTTTCCTGGGGGTAAGCATACTTAACCCGTTAGTTTCC
CCTTATGCCATAACATTAGG

Product: asparaginase

Products: NA

Alternate protein names: L-asparagine amidohydrolase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MVAIIVHGGAGTIRKEERIPKIIEGVREAVLTGWRELKKGSALDAVEEAVKVLEDNPLFNAGTGSVLTLDGKVEMDAAIM
RGKTLDAGAVAGIWGVKNPISVARKVMEKTDHVLLIGEGAVKFARLMGFPEYDPTTEERRKQWEELRKKLLETGEIRHWK
KLSELIKEYPEVLRSTVGAVAFDGEEIVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLSLAKTA
TDFVRLGLDAQAASEAAIRLATKYFGPDTMGIIMVDSNGNVGFAKNTKHMSYAFMKEGMKEPEAGV

Sequences:

>Translated_306_residues
MVAIIVHGGAGTIRKEERIPKIIEGVREAVLTGWRELKKGSALDAVEEAVKVLEDNPLFNAGTGSVLTLDGKVEMDAAIM
RGKTLDAGAVAGIWGVKNPISVARKVMEKTDHVLLIGEGAVKFARLMGFPEYDPTTEERRKQWEELRKKLLETGEIRHWK
KLSELIKEYPEVLRSTVGAVAFDGEEIVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLSLAKTA
TDFVRLGLDAQAASEAAIRLATKYFGPDTMGIIMVDSNGNVGFAKNTKHMSYAFMKEGMKEPEAGV
>Mature_306_residues
MVAIIVHGGAGTIRKEERIPKIIEGVREAVLTGWRELKKGSALDAVEEAVKVLEDNPLFNAGTGSVLTLDGKVEMDAAIM
RGKTLDAGAVAGIWGVKNPISVARKVMEKTDHVLLIGEGAVKFARLMGFPEYDPTTEERRKQWEELRKKLLETGEIRHWK
KLSELIKEYPEVLRSTVGAVAFDGEEIVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLSLAKTA
TDFVRLGLDAQAASEAAIRLATKYFGPDTMGIIMVDSNGNVGFAKNTKHMSYAFMKEGMKEPEAGV

Specific function: Unknown

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family

Homologues:

Organism=Homo sapiens, GI145275202, Length=302, Percent_Identity=41.0596026490066, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI145275200, Length=302, Percent_Identity=41.0596026490066, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI285002251, Length=313, Percent_Identity=31.9488817891374, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI98991769, Length=290, Percent_Identity=32.7586206896552, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI285002253, Length=313, Percent_Identity=30.3514376996805, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1787050, Length=316, Percent_Identity=40.5063291139241, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17569239, Length=243, Percent_Identity=31.6872427983539, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32564649, Length=224, Percent_Identity=33.0357142857143, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18921183, Length=313, Percent_Identity=37.6996805111821, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21357893, Length=261, Percent_Identity=36.3984674329502, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI221330102, Length=307, Percent_Identity=32.2475570032573, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652135, Length=242, Percent_Identity=35.9504132231405, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24657358, Length=277, Percent_Identity=29.2418772563177, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24655553, Length=261, Percent_Identity=28.735632183908, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASGX_PYRFU (Q8U4E6)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_577871.1
- ProteinModelPortal:   Q8U4E6
- SMR:   Q8U4E6
- MEROPS:   T02.002
- EnsemblBacteria:   EBPYRT00000004210
- GeneID:   1467974
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0142
- NMPDR:   fig|186497.1.peg.145
- GeneTree:   EBGT00050000022719
- HOGENOM:   HBG735787
- OMA:   ATGHGEF
- ProtClustDB:   CLSK253204
- BRENDA:   3.5.1.1
- InterPro:   IPR000246
- PANTHER:   PTHR10188

Pfam domain/function: PF01112 Asparaginase_2

EC number: =3.5.1.1

Molecular weight: Translated: 32855; Mature: 32855

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAIIVHGGAGTIRKEERIPKIIEGVREAVLTGWRELKKGSALDAVEEAVKVLEDNPLFN
CEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
AGTGSVLTLDGKVEMDAAIMRGKTLDAGAVAGIWGVKNPISVARKVMEKTDHVLLIGEGA
CCCCCEEEECCCEEHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEECCH
VKFARLMGFPEYDPTTEERRKQWEELRKKLLETGEIRHWKKLSELIKEYPEVLRSTVGAV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHE
AFDGEEIVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLSLAKTA
EECCCEEEEECCCCHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCCCHHHEEEHHHHHH
TDFVRLGLDAQAASEAAIRLATKYFGPDTMGIIMVDSNGNVGFAKNTKHMSYAFMKEGMK
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHCCC
EPEAGV
CCCCCC
>Mature Secondary Structure
MVAIIVHGGAGTIRKEERIPKIIEGVREAVLTGWRELKKGSALDAVEEAVKVLEDNPLFN
CEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
AGTGSVLTLDGKVEMDAAIMRGKTLDAGAVAGIWGVKNPISVARKVMEKTDHVLLIGEGA
CCCCCEEEECCCEEHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEECCH
VKFARLMGFPEYDPTTEERRKQWEELRKKLLETGEIRHWKKLSELIKEYPEVLRSTVGAV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHE
AFDGEEIVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLSLAKTA
EECCCEEEEECCCCHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCCCHHHEEEHHHHHH
TDFVRLGLDAQAASEAAIRLATKYFGPDTMGIIMVDSNGNVGFAKNTKHMSYAFMKEGMK
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHCCC
EPEAGV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA