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The map label for this gene is polB

Identifier: 18976390

GI number: 18976390

Start: 17498

End: 19339

Strand: Direct

Name: polB

Synonym: PF0018

Alternate gene names: 18976390

Gene position: 17498-19339 (Clockwise)

Preceding gene: 18976389

Following gene: 33359447

Centisome position: 0.92

GC content: 41.59

Gene sequence:

>1842_bases
ATGGATGAATTTGTAAAATCACTTCTAAAAGCTAACTATCTAATAACTCCCTCTGCCTACTATCTCTTGAGAGAATACTA
TGAAAAAGGTGAATTCTCAATTGTGGAGCTGGTAAAATTTGCAAGATCAAGAGAGAGCTACATAATTACTGATGCTTTAG
CAACAGAATTCCTTAAAGTTAAAGGCCTTGAACCAATTCTTCCAGTGGAAACAAAGGGGGGTTTTGTTTCCACTGGAGAG
TCCCAAAAAGAGCAGTCTTATGAAGAGTCTTTTGGGACTAAAGAAGAAATTTCCCAGGAGATTAAAGAAGGAGAGAGTTT
TATTTCCACTGGAAGTGAACCACTTGAAGAGGAGCTCAATAGCATTGGAATTGAGGAAATTGGGGCAAATGAAGAGTTAG
TTTCTAATGGAAATGACAATGGTGGAGAGGCAATTGTCTTTGACAAATATGGCTATCCAATGGTATATGCTCCAGAAGAA
ATAGAGGTTGAGGAGAAGGAGTACTCGAAGTATGAAGATCTGACAATACCCATGAACCCCGACTTCAATTATGTGGAAAT
AAAGGAAGATTATGATGTTGTCTTCGATGTTAGGAATGTAAAGCTGAAGCCTCCTAAGGTAAAGAACGGTAATGGGAAGG
AAGGTGAAATAATTGTTGAAGCTTATGCTTCTCTCTTCAGGAGTAGGTTGAAGAAGTTAAGGAAAATACTAAGGGAAAAT
CCTGAATTGGACAATGTTGTTGATATTGGGAAGCTGAAGTATGTGAAGGAAGATGAAACCGTGACAATAATAGGGCTTGT
CAATTCCAAGAGGGAAGTGAATAAAGGATTGATATTTGAAATAGAAGATCTCACAGGAAAGGTTAAAGTTTTCTTGCCGA
AAGATTCGGAAGATTATAGGGAGGCATTTAAGGTTCTTCCAGATGCCGTCGTCGCTTTTAAGGGGGTGTATTCAAAGAGG
GGAATTTTGTACGCCAACAAGTTTTACCTTCCAGACGTTCCCCTCTATAGGAGACAAAAGCCTCCACTGGAAGAGAAAGT
TTATGCTATTCTCATAAGTGATATACACGTCGGAAGTAAAGAGTTCTGCGAAAATGCCTTCATAAAGTTCTTAGAGTGGC
TCAATGGAAACGTTGAAACTAAGGAAGAGGAAGAAATCGTGAGTAGGGTTAAGTATCTAATCATTGCAGGAGATGTTGTT
GATGGTGTTGGCGTTTATCCGGGCCAGTATGCCGACTTGACGATTCCAGATATATTCGACCAGTATGAGGCCCTCGCAAA
CCTTCTCTCTCACGTTCCTAAGCACATAACAATGTTCATTGCCCCAGGAAACCACGATGCTGCTAGGCAAGCTATTCCCC
AACCAGAATTCTACAAAGAGTATGCAAAACCTATATACAAGCTCAAGAACGCCGTGATAATAAGCAATCCTGCTGTAATA
AGACTACATGGTAGGGACTTTCTGATAGCTCATGGTAGGGGGATAGAGGATGTCGTTGGAAGTGTTCCTGGGTTGACCCA
TCACAAGCCCGGCCTCCCAATGGTTGAACTATTGAAGATGAGGCATGTAGCTCCAATGTTTGGAGGAAAGGTTCCAATAG
CTCCTGATCCAGAAGATTTGCTTGTTATAGAAGAAGTTCCTGATGTAGTTCACATGGGTCACGTTCACGTTTACGATGCG
GTAGTTTATAGGGGAGTTCAGCTGGTTAACTCCGCCACCTGGCAGGCTCAGACCGAGTTCCAGAAGATGGTGAACATAGT
TCCAACGCCTGCAAAGGTTCCCGTTGTTGATATTGATACTGCAAAAGTTGTCAAGGTTTTGGACTTTAGTGGGTGGTGCT
GA

Upstream 100 bases:

>100_bases
ACGTTACCTCATATAAGATAAGAAATGTGCTGAGATATGATTACTCTATTCAGCCCCTCCTCACAATTTCCCTTAAGAGT
GAGCAGAGGAGGTTGATCTA

Downstream 100 bases:

>100_bases
TGGAGCTTCCAAAGGAAATTGAGGAGTATTTTGAGATGCTTCAAAGGGAAATTGACAAAGCTTACGAGATTGCTAAGAAG
GCTAGGAGTCAGGGTAAAGA

Product: DNA polymerase II small subunit

Products: NA

Alternate protein names: Pol II; DP1

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MDEFVKSLLKANYLITPSAYYLLREYYEKGEFSIVELVKFARSRESYIITDALATEFLKVKGLEPILPVETKGGFVSTGE
SQKEQSYEESFGTKEEISQEIKEGESFISTGSEPLEEELNSIGIEEIGANEELVSNGNDNGGEAIVFDKYGYPMVYAPEE
IEVEEKEYSKYEDLTIPMNPDFNYVEIKEDYDVVFDVRNVKLKPPKVKNGNGKEGEIIVEAYASLFRSRLKKLRKILREN
PELDNVVDIGKLKYVKEDETVTIIGLVNSKREVNKGLIFEIEDLTGKVKVFLPKDSEDYREAFKVLPDAVVAFKGVYSKR
GILYANKFYLPDVPLYRRQKPPLEEKVYAILISDIHVGSKEFCENAFIKFLEWLNGNVETKEEEEIVSRVKYLIIAGDVV
DGVGVYPGQYADLTIPDIFDQYEALANLLSHVPKHITMFIAPGNHDAARQAIPQPEFYKEYAKPIYKLKNAVIISNPAVI
RLHGRDFLIAHGRGIEDVVGSVPGLTHHKPGLPMVELLKMRHVAPMFGGKVPIAPDPEDLLVIEEVPDVVHMGHVHVYDA
VVYRGVQLVNSATWQAQTEFQKMVNIVPTPAKVPVVDIDTAKVVKVLDFSGWC

Sequences:

>Translated_613_residues
MDEFVKSLLKANYLITPSAYYLLREYYEKGEFSIVELVKFARSRESYIITDALATEFLKVKGLEPILPVETKGGFVSTGE
SQKEQSYEESFGTKEEISQEIKEGESFISTGSEPLEEELNSIGIEEIGANEELVSNGNDNGGEAIVFDKYGYPMVYAPEE
IEVEEKEYSKYEDLTIPMNPDFNYVEIKEDYDVVFDVRNVKLKPPKVKNGNGKEGEIIVEAYASLFRSRLKKLRKILREN
PELDNVVDIGKLKYVKEDETVTIIGLVNSKREVNKGLIFEIEDLTGKVKVFLPKDSEDYREAFKVLPDAVVAFKGVYSKR
GILYANKFYLPDVPLYRRQKPPLEEKVYAILISDIHVGSKEFCENAFIKFLEWLNGNVETKEEEEIVSRVKYLIIAGDVV
DGVGVYPGQYADLTIPDIFDQYEALANLLSHVPKHITMFIAPGNHDAARQAIPQPEFYKEYAKPIYKLKNAVIISNPAVI
RLHGRDFLIAHGRGIEDVVGSVPGLTHHKPGLPMVELLKMRHVAPMFGGKVPIAPDPEDLLVIEEVPDVVHMGHVHVYDA
VVYRGVQLVNSATWQAQTEFQKMVNIVPTPAKVPVVDIDTAKVVKVLDFSGWC
>Mature_613_residues
MDEFVKSLLKANYLITPSAYYLLREYYEKGEFSIVELVKFARSRESYIITDALATEFLKVKGLEPILPVETKGGFVSTGE
SQKEQSYEESFGTKEEISQEIKEGESFISTGSEPLEEELNSIGIEEIGANEELVSNGNDNGGEAIVFDKYGYPMVYAPEE
IEVEEKEYSKYEDLTIPMNPDFNYVEIKEDYDVVFDVRNVKLKPPKVKNGNGKEGEIIVEAYASLFRSRLKKLRKILREN
PELDNVVDIGKLKYVKEDETVTIIGLVNSKREVNKGLIFEIEDLTGKVKVFLPKDSEDYREAFKVLPDAVVAFKGVYSKR
GILYANKFYLPDVPLYRRQKPPLEEKVYAILISDIHVGSKEFCENAFIKFLEWLNGNVETKEEEEIVSRVKYLIIAGDVV
DGVGVYPGQYADLTIPDIFDQYEALANLLSHVPKHITMFIAPGNHDAARQAIPQPEFYKEYAKPIYKLKNAVIISNPAVI
RLHGRDFLIAHGRGIEDVVGSVPGLTHHKPGLPMVELLKMRHVAPMFGGKVPIAPDPEDLLVIEEVPDVVHMGHVHVYDA
VVYRGVQLVNSATWQAQTEFQKMVNIVPTPAKVPVVDIDTAKVVKVLDFSGWC

Specific function: Possesses two activities:a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase

COG id: COG1311

COG function: function code L; Archaeal DNA polymerase II, small subunit/DNA polymerase delta, subunit B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase delta/II small subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DP2S_PYRFU (P81412)

Other databases:

- EMBL:   D84670
- EMBL:   AE009950
- PIR:   T43933
- RefSeq:   NP_577747.1
- ProteinModelPortal:   P81412
- EnsemblBacteria:   EBPYRT00000005111
- GeneID:   1467846
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0018
- NMPDR:   fig|186497.1.peg.18
- GeneTree:   EBGT00050000022734
- HOGENOM:   HBG726778
- OMA:   IPDIFDQ
- ProtClustDB:   PRK04036
- BRENDA:   2.7.7.7
- HAMAP:   MF_00325
- InterPro:   IPR007185
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR011149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF000803

Pfam domain/function: PF04042 DNA_pol_E_B; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =2.7.7.7

Molecular weight: Translated: 69185; Mature: 69185

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEFVKSLLKANYLITPSAYYLLREYYEKGEFSIVELVKFARSRESYIITDALATEFLKV
CHHHHHHHHHCCEEECCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
KGLEPILPVETKGGFVSTGESQKEQSYEESFGTKEEISQEIKEGESFISTGSEPLEEELN
CCCCCCCEECCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
SIGIEEIGANEELVSNGNDNGGEAIVFDKYGYPMVYAPEEIEVEEKEYSKYEDLTIPMNP
HCCHHHCCCCHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCCCHHHCCCCCCEEEECCC
DFNYVEIKEDYDVVFDVRNVKLKPPKVKNGNGKEGEIIVEAYASLFRSRLKKLRKILREN
CCCEEEEECCCEEEEEEECCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PELDNVVDIGKLKYVKEDETVTIIGLVNSKREVNKGLIFEIEDLTGKVKVFLPKDSEDYR
CCCCCCCCCCCEEEEECCCEEEEEEEECCCHHHCCCCEEEEECCCCEEEEEECCCCHHHH
EAFKVLPDAVVAFKGVYSKRGILYANKFYLPDVPLYRRQKPPLEEKVYAILISDIHVGSK
HHHHHHHHHHHHHHHHHHCCCEEEECCEECCCCCHHCCCCCCHHHHEEEEEEECCCCCCH
EFCENAFIKFLEWLNGNVETKEEEEIVSRVKYLIIAGDVVDGVGVYPGQYADLTIPDIFD
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCHHCCCCCCCCCCCCCCCHHHHH
QYEALANLLSHVPKHITMFIAPGNHDAARQAIPQPEFYKEYAKPIYKLKNAVIISNPAVI
HHHHHHHHHHHCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCEEE
RLHGRDFLIAHGRGIEDVVGSVPGLTHHKPGLPMVELLKMRHVAPMFGGKVPIAPDPEDL
EECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCE
LVIEEVPDVVHMGHVHVYDAVVYRGVQLVNSATWQAQTEFQKMVNIVPTPAKVPVVDIDT
EEEECCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEECCH
AKVVKVLDFSGWC
HHHHHHHCCCCCC
>Mature Secondary Structure
MDEFVKSLLKANYLITPSAYYLLREYYEKGEFSIVELVKFARSRESYIITDALATEFLKV
CHHHHHHHHHCCEEECCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
KGLEPILPVETKGGFVSTGESQKEQSYEESFGTKEEISQEIKEGESFISTGSEPLEEELN
CCCCCCCEECCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
SIGIEEIGANEELVSNGNDNGGEAIVFDKYGYPMVYAPEEIEVEEKEYSKYEDLTIPMNP
HCCHHHCCCCHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCCCHHHCCCCCCEEEECCC
DFNYVEIKEDYDVVFDVRNVKLKPPKVKNGNGKEGEIIVEAYASLFRSRLKKLRKILREN
CCCEEEEECCCEEEEEEECCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PELDNVVDIGKLKYVKEDETVTIIGLVNSKREVNKGLIFEIEDLTGKVKVFLPKDSEDYR
CCCCCCCCCCCEEEEECCCEEEEEEEECCCHHHCCCCEEEEECCCCEEEEEECCCCHHHH
EAFKVLPDAVVAFKGVYSKRGILYANKFYLPDVPLYRRQKPPLEEKVYAILISDIHVGSK
HHHHHHHHHHHHHHHHHHCCCEEEECCEECCCCCHHCCCCCCHHHHEEEEEEECCCCCCH
EFCENAFIKFLEWLNGNVETKEEEEIVSRVKYLIIAGDVVDGVGVYPGQYADLTIPDIFD
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCHHCCCCCCCCCCCCCCCHHHHH
QYEALANLLSHVPKHITMFIAPGNHDAARQAIPQPEFYKEYAKPIYKLKNAVIISNPAVI
HHHHHHHHHHHCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCEEE
RLHGRDFLIAHGRGIEDVVGSVPGLTHHKPGLPMVELLKMRHVAPMFGGKVPIAPDPEDL
EECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCE
LVIEEVPDVVHMGHVHVYDAVVYRGVQLVNSATWQAQTEFQKMVNIVPTPAKVPVVDIDT
EEEECCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEECCH
AKVVKVLDFSGWC
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9348040