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The map label for this gene is aam1 [H]

Identifier: 189499219

GI number: 189499219

Start: 227817

End: 229937

Strand: Direct

Name: aam1 [H]

Synonym: Cphamn1_0239

Alternate gene names: 189499219

Gene position: 227817-229937 (Clockwise)

Preceding gene: 189499212

Following gene: 189499220

Centisome position: 8.33

GC content: 51.16

Gene sequence:

>2121_bases
ATGGATTACTTGATGAGTGAAACAAAAAATCAATTTTTTTCACATCATGCAACCACATCTTTTCCCTATTTTGCATCAGG
TGTAAACCTCTATACCATTATACATATGAATCACACATTTTACCAGCATAAACCTGTAATCAGCAGCCGGAAGTTCGACG
GCAGGAGGCGTGTCATCATTGATCGTGTGTCTCCTGAGATAGACGGCGGCCAATACCCTGTGAAGAGAATCCCGGGGGAA
ACGGTTCGGGTGGAGGCTGATATTTTTACTGACGGTGCTGACGTTATCAGCGCCGAGATTTGTTACCGGTCCGCATCGGC
CCGTGCCTGGAAACGCTCTCCCATGATAGCGCTTTTCAATGACGCCTGGGAAGGGGAGTTTGCCGTTGGCGATCCTGGGA
ACTACGAGTTCACTATCGAAGCTTGGATCGATCATTTTCAGACATGGCGGAGAGGGCTGATAAAAAAAATCGAGGCCGGT
CAGGATGTTTCCCTTGCATTTCGTATTGGCGCGGGGATAGTTGAAAAAGCGGTTGAGCGAGCATCAAAAAGTGATGGCGC
AAGGATGAGAGCCCTGATTTCCGTGATGACTTCGGGGGATGCCGAGGAAGCTGCTTCGACGGCACTGTCCGACGAGCTTG
ACGGGCTGATGCAGTCCTGCCCGGACAAGGAGGCGGCCTCGCAGTATAACAGGGTGCTCAAGGTCAGGGTTGACCAGAAA
AAGGCCGGGTTCAGTACCTGGTATGAATTTTTCCCCCGCTCGTGGGCCGTGGAACCGGGCAGGCACGGAACGTTCAGGGA
GTGTAAACGTCTGCTTCCTCTCATTGCCCGGATGGGATTTGATGTTGTCTATCTTCCTCCGATTTATCCGATCGGTTCGA
CAAAGCGTAAAGGAAAAAACAACACTCTCGTCGCAGGGCCTGACGACCCCGGGAGCTGCTGGGCGATTGGAAGCGGTGAA
GGGGGGCATAAGTCTGTCCATCCTGAACTGGGCACCATAGAAGATTTCGGGGAATTTGTCCGGGAAGCAGAAGCCGAAGG
CATCTCCGTGGCTCTGGATATTGCGTTCCAGTGCTCCCCGGACCATCCCTATGTGAAAGAGCACCCTCAATGGTTCAAAT
GGCGTCCTGACGGTACGGTTCAGTTCGCGGAGAATCCGCCGAAAAGGTATGAGGATATTCTTCCGCTTGATTTCGAGACG
GAGGACTGGAAAAATCTCTGGACCGAACTGAAAAGTGTTCTGCTTTTCTGGATTGAAAAAGGGGTCAGGATCTTCAGGGT
TGACAATCCGCACACCAAGGCTTTTCTTTTCTGGGACTGGGTGATTGACGAGATTACCGGAGAGTATCCCGATACCGTTT
TTCTTGCTGAAGCGTTTACCCGTCCGAAGGTAATGGCAAGACTTGCCAAAGGGGGGTTCAATCAGTCGTATACCTACTTT
ACCTGGAGAAACACCAAGCACGACCTTCAAAAGTATCTCACGGAACTGACCACGTCGGAAGTGAAGGAGTACATGAGGCC
GAACTTCTGGCCGAATACGCCCGACATTCTGCATGAAGAGCTGCAGACCGGAAGTCGAGCTACCTTTCTTACCCGAATGG
CGCTTGCGGCAACCCTTTCTTCCAACTACGGGATGTACGCTCCTGCATATGAGCTATGCGAGTACCTGCCTGTAAAGCCA
GGCAAGGAGGAGTATCTCGATTCGGAGAAGTACGAGATCAAGCAATGGGATATGGACAGGCCTAGCAATATCCGCGCCGA
GATTACGGCGGTTAACCATATCCGCAGGGAGAACGCCGCCCTGCAGCAGACAAATAACATAGAATTTGTAAGGGTTGACG
CCGGTGAGGGGCGTGAACATGATATGCTGATGGGTTATGTGAAACGATCCGTGGATGACAGTAACATTATCCTGACGGTT
GTGAATCTCGATCCTCAGAACACGCATGCCGGATGGCTGCGTTTTCCACTTGAAGCGTTCAAGTTACCGCATACCCATCA
TTTCAGGGTTGAAGACCTTATGAGCGGGCGCACCTTCGAGTGGGATGGCGAATGGAACTACGTAGAGCTGAATCCTGAAC
ATATGCCGGTACACATTTTTAAAGTCAACCTGTTTTCTTAG

Upstream 100 bases:

>100_bases
GTGTATCTCGTGCAGACGCTCTTTTGCTCCTTCAAACATGGTATTTCCTTTCTTCCTCTCGTTGTGAAATGTTTTTAAAC
CGCGCTGTTTTGCAGGTTTC

Downstream 100 bases:

>100_bases
CAGAGGCCTTTCTGCAGATCAATAACTAACGTTTTCGATTTTTCCTGGAAATTCCGATACCATTGCGGGACTTCCAGACG
TATCACCAATTATATCAGTT

Product: alpha amylase catalytic subunit

Products: D-Glucose 6-phosphate; D-Glucose [C]

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MDYLMSETKNQFFSHHATTSFPYFASGVNLYTIIHMNHTFYQHKPVISSRKFDGRRRVIIDRVSPEIDGGQYPVKRIPGE
TVRVEADIFTDGADVISAEICYRSASARAWKRSPMIALFNDAWEGEFAVGDPGNYEFTIEAWIDHFQTWRRGLIKKIEAG
QDVSLAFRIGAGIVEKAVERASKSDGARMRALISVMTSGDAEEAASTALSDELDGLMQSCPDKEAASQYNRVLKVRVDQK
KAGFSTWYEFFPRSWAVEPGRHGTFRECKRLLPLIARMGFDVVYLPPIYPIGSTKRKGKNNTLVAGPDDPGSCWAIGSGE
GGHKSVHPELGTIEDFGEFVREAEAEGISVALDIAFQCSPDHPYVKEHPQWFKWRPDGTVQFAENPPKRYEDILPLDFET
EDWKNLWTELKSVLLFWIEKGVRIFRVDNPHTKAFLFWDWVIDEITGEYPDTVFLAEAFTRPKVMARLAKGGFNQSYTYF
TWRNTKHDLQKYLTELTTSEVKEYMRPNFWPNTPDILHEELQTGSRATFLTRMALAATLSSNYGMYAPAYELCEYLPVKP
GKEEYLDSEKYEIKQWDMDRPSNIRAEITAVNHIRRENAALQQTNNIEFVRVDAGEGREHDMLMGYVKRSVDDSNIILTV
VNLDPQNTHAGWLRFPLEAFKLPHTHHFRVEDLMSGRTFEWDGEWNYVELNPEHMPVHIFKVNLFS

Sequences:

>Translated_706_residues
MDYLMSETKNQFFSHHATTSFPYFASGVNLYTIIHMNHTFYQHKPVISSRKFDGRRRVIIDRVSPEIDGGQYPVKRIPGE
TVRVEADIFTDGADVISAEICYRSASARAWKRSPMIALFNDAWEGEFAVGDPGNYEFTIEAWIDHFQTWRRGLIKKIEAG
QDVSLAFRIGAGIVEKAVERASKSDGARMRALISVMTSGDAEEAASTALSDELDGLMQSCPDKEAASQYNRVLKVRVDQK
KAGFSTWYEFFPRSWAVEPGRHGTFRECKRLLPLIARMGFDVVYLPPIYPIGSTKRKGKNNTLVAGPDDPGSCWAIGSGE
GGHKSVHPELGTIEDFGEFVREAEAEGISVALDIAFQCSPDHPYVKEHPQWFKWRPDGTVQFAENPPKRYEDILPLDFET
EDWKNLWTELKSVLLFWIEKGVRIFRVDNPHTKAFLFWDWVIDEITGEYPDTVFLAEAFTRPKVMARLAKGGFNQSYTYF
TWRNTKHDLQKYLTELTTSEVKEYMRPNFWPNTPDILHEELQTGSRATFLTRMALAATLSSNYGMYAPAYELCEYLPVKP
GKEEYLDSEKYEIKQWDMDRPSNIRAEITAVNHIRRENAALQQTNNIEFVRVDAGEGREHDMLMGYVKRSVDDSNIILTV
VNLDPQNTHAGWLRFPLEAFKLPHTHHFRVEDLMSGRTFEWDGEWNYVELNPEHMPVHIFKVNLFS
>Mature_706_residues
MDYLMSETKNQFFSHHATTSFPYFASGVNLYTIIHMNHTFYQHKPVISSRKFDGRRRVIIDRVSPEIDGGQYPVKRIPGE
TVRVEADIFTDGADVISAEICYRSASARAWKRSPMIALFNDAWEGEFAVGDPGNYEFTIEAWIDHFQTWRRGLIKKIEAG
QDVSLAFRIGAGIVEKAVERASKSDGARMRALISVMTSGDAEEAASTALSDELDGLMQSCPDKEAASQYNRVLKVRVDQK
KAGFSTWYEFFPRSWAVEPGRHGTFRECKRLLPLIARMGFDVVYLPPIYPIGSTKRKGKNNTLVAGPDDPGSCWAIGSGE
GGHKSVHPELGTIEDFGEFVREAEAEGISVALDIAFQCSPDHPYVKEHPQWFKWRPDGTVQFAENPPKRYEDILPLDFET
EDWKNLWTELKSVLLFWIEKGVRIFRVDNPHTKAFLFWDWVIDEITGEYPDTVFLAEAFTRPKVMARLAKGGFNQSYTYF
TWRNTKHDLQKYLTELTTSEVKEYMRPNFWPNTPDILHEELQTGSRATFLTRMALAATLSSNYGMYAPAYELCEYLPVKP
GKEEYLDSEKYEIKQWDMDRPSNIRAEITAVNHIRRENAALQQTNNIEFVRVDAGEGREHDMLMGYVKRSVDDSNIILTV
VNLDPQNTHAGWLRFPLEAFKLPHTHHFRVEDLMSGRTFEWDGEWNYVELNPEHMPVHIFKVNLFS

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF11896 DUF3416 [H]

EC number: 3.2.1.93 [C]

Molecular weight: Translated: 81077; Mature: 81077

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00019 ACTININ_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLMSETKNQFFSHHATTSFPYFASGVNLYTIIHMNHTFYQHKPVISSRKFDGRRRVII
CCCHHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
DRVSPEIDGGQYPVKRIPGETVRVEADIFTDGADVISAEICYRSASARAWKRSPMIALFN
EECCCCCCCCCCCHHHCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCHHHCCCEEEEEC
DAWEGEFAVGDPGNYEFTIEAWIDHFQTWRRGLIKKIEAGQDVSLAFRIGAGIVEKAVER
CCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
ASKSDGARMRALISVMTSGDAEEAASTALSDELDGLMQSCPDKEAASQYNRVLKVRVDQK
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEECCC
KAGFSTWYEFFPRSWAVEPGRHGTFRECKRLLPLIARMGFDVVYLPPIYPIGSTKRKGKN
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
NTLVAGPDDPGSCWAIGSGEGGHKSVHPELGTIEDFGEFVREAEAEGISVALDIAFQCSP
CCEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECC
DHPYVKEHPQWFKWRPDGTVQFAENPPKRYEDILPLDFETEDWKNLWTELKSVLLFWIEK
CCCCHHCCCCCEEECCCCCEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GVRIFRVDNPHTKAFLFWDWVIDEITGEYPDTVFLAEAFTRPKVMARLAKGGFNQSYTYF
CCEEEEECCCCCEEEEEHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHCCCCCCEEEE
TWRNTKHDLQKYLTELTTSEVKEYMRPNFWPNTPDILHEELQTGSRATFLTRMALAATLS
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
SNYGMYAPAYELCEYLPVKPGKEEYLDSEKYEIKQWDMDRPSNIRAEITAVNHIRRENAA
CCCCCCCCHHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHH
LQQTNNIEFVRVDAGEGREHDMLMGYVKRSVDDSNIILTVVNLDPQNTHAGWLRFPLEAF
HHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEECCHHHH
KLPHTHHFRVEDLMSGRTFEWDGEWNYVELNPEHMPVHIFKVNLFS
CCCCCCCEEHHHHHCCCEEEECCCCCEEEECCCCCEEEEEEEEECC
>Mature Secondary Structure
MDYLMSETKNQFFSHHATTSFPYFASGVNLYTIIHMNHTFYQHKPVISSRKFDGRRRVII
CCCHHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
DRVSPEIDGGQYPVKRIPGETVRVEADIFTDGADVISAEICYRSASARAWKRSPMIALFN
EECCCCCCCCCCCHHHCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCHHHCCCEEEEEC
DAWEGEFAVGDPGNYEFTIEAWIDHFQTWRRGLIKKIEAGQDVSLAFRIGAGIVEKAVER
CCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
ASKSDGARMRALISVMTSGDAEEAASTALSDELDGLMQSCPDKEAASQYNRVLKVRVDQK
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEECCC
KAGFSTWYEFFPRSWAVEPGRHGTFRECKRLLPLIARMGFDVVYLPPIYPIGSTKRKGKN
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
NTLVAGPDDPGSCWAIGSGEGGHKSVHPELGTIEDFGEFVREAEAEGISVALDIAFQCSP
CCEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECC
DHPYVKEHPQWFKWRPDGTVQFAENPPKRYEDILPLDFETEDWKNLWTELKSVLLFWIEK
CCCCHHCCCCCEEECCCCCEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GVRIFRVDNPHTKAFLFWDWVIDEITGEYPDTVFLAEAFTRPKVMARLAKGGFNQSYTYF
CCEEEEECCCCCEEEEEHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHCCCCCCEEEE
TWRNTKHDLQKYLTELTTSEVKEYMRPNFWPNTPDILHEELQTGSRATFLTRMALAATLS
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
SNYGMYAPAYELCEYLPVKPGKEEYLDSEKYEIKQWDMDRPSNIRAEITAVNHIRRENAA
CCCCCCCCHHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHH
LQQTNNIEFVRVDAGEGREHDMLMGYVKRSVDDSNIILTVVNLDPQNTHAGWLRFPLEAF
HHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEECCHHHH
KLPHTHHFRVEDLMSGRTFEWDGEWNYVELNPEHMPVHIFKVNLFS
CCCCCCCEEHHHHHCCCEEEECCCCCEEEECCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; alpha,alpha'-Trehalose 6-phosphate [C]

Specific reaction: H2O + alpha,alpha'-Trehalose 6-phosphate --> D-Glucose 6-phosphate + D-Glucose [C]

General reaction: O-Glycosyl bond hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10970853 [H]