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The map label for this gene is rnhB [H]

Identifier: 189499018

GI number: 189499018

Start: 21575

End: 22198

Strand: Reverse

Name: rnhB [H]

Synonym: Cphamn1_0018

Alternate gene names: 189499018

Gene position: 22198-21575 (Counterclockwise)

Preceding gene: 189499022

Following gene: 189499017

Centisome position: 0.81

GC content: 51.92

Gene sequence:

>624_bases
TTGCTACTGTCAACCGAAGACTATGAGGAAAAATTCTGGCATGAACACACATGCGTCTGCGGCATAGACGAGGCGGGCAG
GGGACCTCTTGCCGGCCCGGTCGTGGCCGCCGCGGTTATGTTCCCCCGCTATTTCAAGCCAGAAGGCGTGCTGACCGGAC
TGAACGATTCCAAAACTCTCCGTCCGAAACAGCGATCGGCATTGGCTGAAGCGATACAAAGCGCGGCTGAATGCTTCAGC
ATATCGATTATAGACCATACCACTATCGACAGTGTGAACATTTTCAACGCAACCATGCTCGCGATGAACGCTGCAGCAGA
AAAGCTCGAGCAACTGCCTGGTCTTTTGCTGATTGACGGAAACCGTTTTGTCCCGAGCCTTCCCATCCCTTACCTGACCA
TTGTCAAAGGAGACACAAAGGTAGTTTCCATCGCGGCAGCCTCCATTCTTGCCAAGACCTGTCGCGACAGAATAATGAAT
GAATACCACCGGGAATATCCTGAATACGGCTTTGCACAACATTTTGGCTACCCGACACAAGCGCATATAGCAGCCATTGC
CCGATACGGGCGCTGTCCCATTCACAGAACAAGCTTCAGGGTAAAACAACTCGGCGAAAAGTAA

Upstream 100 bases:

>100_bases
TGAAATGTAACCTGTATTATTTAACATTTTTGTCAAAAGGACAAATTTTCGTCTTTTTTCCTTGCAGACAACGCCGAGCC
AACACTAAACGGACGAACCA

Downstream 100 bases:

>100_bases
ACGGAGCTGAATCATGTCTTTTATTCCCCACGACCTCGGACGCCAGGGAGAACATACAGCCGTCACCTTCCTCATAGAAA
AAGGGTACCGGATTCTCCAG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MLLSTEDYEEKFWHEHTCVCGIDEAGRGPLAGPVVAAAVMFPRYFKPEGVLTGLNDSKTLRPKQRSALAEAIQSAAECFS
ISIIDHTTIDSVNIFNATMLAMNAAAEKLEQLPGLLLIDGNRFVPSLPIPYLTIVKGDTKVVSIAAASILAKTCRDRIMN
EYHREYPEYGFAQHFGYPTQAHIAAIARYGRCPIHRTSFRVKQLGEK

Sequences:

>Translated_207_residues
MLLSTEDYEEKFWHEHTCVCGIDEAGRGPLAGPVVAAAVMFPRYFKPEGVLTGLNDSKTLRPKQRSALAEAIQSAAECFS
ISIIDHTTIDSVNIFNATMLAMNAAAEKLEQLPGLLLIDGNRFVPSLPIPYLTIVKGDTKVVSIAAASILAKTCRDRIMN
EYHREYPEYGFAQHFGYPTQAHIAAIARYGRCPIHRTSFRVKQLGEK
>Mature_207_residues
MLLSTEDYEEKFWHEHTCVCGIDEAGRGPLAGPVVAAAVMFPRYFKPEGVLTGLNDSKTLRPKQRSALAEAIQSAAECFS
ISIIDHTTIDSVNIFNATMLAMNAAAEKLEQLPGLLLIDGNRFVPSLPIPYLTIVKGDTKVVSIAAASILAKTCRDRIMN
EYHREYPEYGFAQHFGYPTQAHIAAIARYGRCPIHRTSFRVKQLGEK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=190, Percent_Identity=47.8947368421053, Blast_Score=159, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 22913; Mature: 22913

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSTEDYEEKFWHEHTCVCGIDEAGRGPLAGPVVAAAVMFPRYFKPEGVLTGLNDSKTL
CCCCCCCHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCC
RPKQRSALAEAIQSAAECFSISIIDHTTIDSVNIFNATMLAMNAAAEKLEQLPGLLLIDG
CHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
NRFVPSLPIPYLTIVKGDTKVVSIAAASILAKTCRDRIMNEYHREYPEYGFAQHFGYPTQ
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHH
AHIAAIARYGRCPIHRTSFRVKQLGEK
HHHHHHHHHCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MLLSTEDYEEKFWHEHTCVCGIDEAGRGPLAGPVVAAAVMFPRYFKPEGVLTGLNDSKTL
CCCCCCCHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCC
RPKQRSALAEAIQSAAECFSISIIDHTTIDSVNIFNATMLAMNAAAEKLEQLPGLLLIDG
CHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
NRFVPSLPIPYLTIVKGDTKVVSIAAASILAKTCRDRIMNEYHREYPEYGFAQHFGYPTQ
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHH
AHIAAIARYGRCPIHRTSFRVKQLGEK
HHHHHHHHHCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA