Click here to switch to the map view.

The map label for this gene is ribH

Identifier: 189424281

GI number: 189424281

Start: 1262310

End: 1262777

Strand: Reverse

Name: ribH

Synonym: Glov_1216

Alternate gene names: 189424281

Gene position: 1262777-1262310 (Counterclockwise)

Preceding gene: 189424282

Following gene: 189424280

Centisome position: 32.23

GC content: 56.41

Gene sequence:

>468_bases
ATGCCCAAGTTTATTGAAGGCAATCTCGACGCAAAAGGCCTGACCTTTGGTATTGTGGTCAGCCGTTTTAACAGTTTTAT
CTGTGAGCGCCTGCTGGAAGGGGCGATTGATGCCCTGGTGCGTCACGGCGCCAATGAAACCGATATCACCGTGGCACGTA
TCCCCGGTGCCTTTGAGATTCCCCTGGCAGCCAAGACCATGGCAGCCTCTCAGAAGTTTGATGCCGTGATCTGCCTGGGT
GCGGTCATCCGCGGTTCCACCCCCCATTTTGAGTATGTCTCTTCCGAGGTATCCAAAGGGGTTGCGTCGGTCTCTCTGGA
AAGCGGACTGCCGATCGCCTTTGGCGTGCTGACCACCGACACCATTGAACAGGCCGTGGAGCGGGCCGGCACCAAGGCGG
GCAACAAGGGTTTTGAGGCCGCGGTTACCGCCATTGAGACCGTCAACGTGATTAAGGCGATGAAATAA

Upstream 100 bases:

>100_bases
CCGGCAGCTGCATGAAAATCTATCCCCATTGCCACAAAATATGCTATAGAAAAAAACTTTCAGATGAATGAAATTGTGAA
AACTCATTGAGGAGTCTCCC

Downstream 100 bases:

>100_bases
GATGGGACTTCGTCGCGAAGCACGTGAACTGGCGCTGCAGATGCTCTACGCACAGGATACCCTGCAGGGCAGCCTGCGGG
ACACCCTGCGGGGCTTCCGC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MPKFIEGNLDAKGLTFGIVVSRFNSFICERLLEGAIDALVRHGANETDITVARIPGAFEIPLAAKTMAASQKFDAVICLG
AVIRGSTPHFEYVSSEVSKGVASVSLESGLPIAFGVLTTDTIEQAVERAGTKAGNKGFEAAVTAIETVNVIKAMK

Sequences:

>Translated_155_residues
MPKFIEGNLDAKGLTFGIVVSRFNSFICERLLEGAIDALVRHGANETDITVARIPGAFEIPLAAKTMAASQKFDAVICLG
AVIRGSTPHFEYVSSEVSKGVASVSLESGLPIAFGVLTTDTIEQAVERAGTKAGNKGFEAAVTAIETVNVIKAMK
>Mature_154_residues
PKFIEGNLDAKGLTFGIVVSRFNSFICERLLEGAIDALVRHGANETDITVARIPGAFEIPLAAKTMAASQKFDAVICLGA
VIRGSTPHFEYVSSEVSKGVASVSLESGLPIAFGVLTTDTIEQAVERAGTKAGNKGFEAAVTAIETVNVIKAMK

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=57.1428571428571, Blast_Score=175, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324429, Length=146, Percent_Identity=32.8767123287671, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_GEOLS (B3E758)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001951458.1
- ProteinModelPortal:   B3E758
- SMR:   B3E758
- GeneID:   6368112
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_1216
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16272; Mature: 16141

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKFIEGNLDAKGLTFGIVVSRFNSFICERLLEGAIDALVRHGANETDITVARIPGAFEI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEC
PLAAKTMAASQKFDAVICLGAVIRGSTPHFEYVSSEVSKGVASVSLESGLPIAFGVLTTD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEHHHHHH
TIEQAVERAGTKAGNKGFEAAVTAIETVNVIKAMK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKFIEGNLDAKGLTFGIVVSRFNSFICERLLEGAIDALVRHGANETDITVARIPGAFEI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEC
PLAAKTMAASQKFDAVICLGAVIRGSTPHFEYVSSEVSKGVASVSLESGLPIAFGVLTTD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEHHHHHH
TIEQAVERAGTKAGNKGFEAAVTAIETVNVIKAMK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA