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The map label for this gene is miaA1

Identifier: 189424058

GI number: 189424058

Start: 1030206

End: 1031123

Strand: Reverse

Name: miaA1

Synonym: Glov_0992

Alternate gene names: 189424058

Gene position: 1031123-1030206 (Counterclockwise)

Preceding gene: 189424059

Following gene: 189424057

Centisome position: 26.32

GC content: 59.8

Gene sequence:

>918_bases
ATGAAGCCCCGTGTACTGGTCATTGCCGGCCCCACGGCATCGGGTAAATCAGCGTTGGCCCTTGATCTGGCTGTGGCGTT
GGACGGAGAGATCATCTGCGCCGACTCCCTGACGGTCTACCGCGGCCTGGATATCGGCAGTGCCAAACCGACCGCAGAGC
AACAGCGGCAGATCCCCCATCACCTGCTGGATATCCGCGAGCCAACCGAACCGTTTACCGCTGCTGACTTCAAGCTTGCT
GCAAAAGCGGCCATTGCCGCTATCTGCCAGCGGGGCAAGCGGCCGATCCTGGCCGGCGGTACCGGCCTGTACCTGCGTGC
ACTGCTGCGGGGACTGAATGATGCGCCGGGGGAAGATCCGGTCCTGCGGGAGACACTGCGAAAGCGGTTGGAGTGCGAGG
GTGCAGAAACTCTGCTGGCAGAGCTGGCCAAGGTAGACCCGGATACCTCACAGCGATTGCATCCCAACAACCGCAACCGG
ATTATCCGCGCCCTGGAGGTTTTTCAGACCACCGGCATTCCGCTGTCACAGTTTCAGGCAGAGCACGGTTTTGCCGATAG
CCCCTACGACAGCCTGCAGTTCTGCCTTGATCTGCCTCGCCCGGAGCTGTACCAACGGATTGATGACCGGGTAGATGCCA
TGCTTGCAGCCGGTCTGGTGGCCGAGGTACAGGGATTACTGCAATCCGGTGTACCGGCAGACTGCAAGCCGTTGCAGGCC
ATCGGCTACAAAGAGGTGCTGGCCCACCTGCAGGGTGAGTACGATCACAACGAGATGGTCAGGCTGATCAAGCGCAATAC
CCGTCACTTTGCCAAACGCCAGTTGACCTGGTTCAGAAGCGAACCCGCAATGCAGTGGGTTGCATATCCGGAAAACTCTG
CTACTATCCATTCTGCTGCAGCCACGTTCTTCGCCTAA

Upstream 100 bases:

>100_bases
GGGCGATGGACCGTACCGATTTCTCGGCACACTGTCCCCATGGCCGGCCGGTCTCCCATGAGATCACCCTGCGAGAACTG
GAAAAGTTCTTCAACCGGCC

Downstream 100 bases:

>100_bases
AGGAGAAAGAAGATTATGTCCAAAGCCCCCTTCAATATCCAGGACCAGTACCTGAACCAGTCCCGCAAAGAGCGTGTCAA
GGTGGTTGTCCGCATGATGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 1; DMAPP:tRNA dimethylallyltransferase 1; DMATase 1; Isopentenyl-diphosphate:tRNA isopentenyltransferase 1; IPP transferase 1; IPPT 1; IPTase 1

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKPRVLVIAGPTASGKSALALDLAVALDGEIICADSLTVYRGLDIGSAKPTAEQQRQIPHHLLDIREPTEPFTAADFKLA
AKAAIAAICQRGKRPILAGGTGLYLRALLRGLNDAPGEDPVLRETLRKRLECEGAETLLAELAKVDPDTSQRLHPNNRNR
IIRALEVFQTTGIPLSQFQAEHGFADSPYDSLQFCLDLPRPELYQRIDDRVDAMLAAGLVAEVQGLLQSGVPADCKPLQA
IGYKEVLAHLQGEYDHNEMVRLIKRNTRHFAKRQLTWFRSEPAMQWVAYPENSATIHSAAATFFA

Sequences:

>Translated_305_residues
MKPRVLVIAGPTASGKSALALDLAVALDGEIICADSLTVYRGLDIGSAKPTAEQQRQIPHHLLDIREPTEPFTAADFKLA
AKAAIAAICQRGKRPILAGGTGLYLRALLRGLNDAPGEDPVLRETLRKRLECEGAETLLAELAKVDPDTSQRLHPNNRNR
IIRALEVFQTTGIPLSQFQAEHGFADSPYDSLQFCLDLPRPELYQRIDDRVDAMLAAGLVAEVQGLLQSGVPADCKPLQA
IGYKEVLAHLQGEYDHNEMVRLIKRNTRHFAKRQLTWFRSEPAMQWVAYPENSATIHSAAATFFA
>Mature_305_residues
MKPRVLVIAGPTASGKSALALDLAVALDGEIICADSLTVYRGLDIGSAKPTAEQQRQIPHHLLDIREPTEPFTAADFKLA
AKAAIAAICQRGKRPILAGGTGLYLRALLRGLNDAPGEDPVLRETLRKRLECEGAETLLAELAKVDPDTSQRLHPNNRNR
IIRALEVFQTTGIPLSQFQAEHGFADSPYDSLQFCLDLPRPELYQRIDDRVDAMLAAGLVAEVQGLLQSGVPADCKPLQA
IGYKEVLAHLQGEYDHNEMVRLIKRNTRHFAKRQLTWFRSEPAMQWVAYPENSATIHSAAATFFA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=307, Percent_Identity=34.2019543973941, Blast_Score=146, Evalue=2e-35,
Organism=Escherichia coli, GI1790613, Length=287, Percent_Identity=41.8118466898955, Blast_Score=222, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI25144712, Length=271, Percent_Identity=29.8892988929889, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=34.1296928327645, Blast_Score=166, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24649830, Length=266, Percent_Identity=37.9699248120301, Blast_Score=155, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA1_GEOLS (B3E5Z1)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001951235.1
- ProteinModelPortal:   B3E5Z1
- SMR:   B3E5Z1
- GeneID:   6369138
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_0992
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33550; Mature: 33550

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRVLVIAGPTASGKSALALDLAVALDGEIICADSLTVYRGLDIGSAKPTAEQQRQIPH
CCCCEEEEECCCCCCCCHHHEEEEEEECCCEEEECCEEHHCCCCCCCCCCCHHHHHCCHH
HLLDIREPTEPFTAADFKLAAKAAIAAICQRGKRPILAGGTGLYLRALLRGLNDAPGEDP
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCCH
VLRETLRKRLECEGAETLLAELAKVDPDTSQRLHPNNRNRIIRALEVFQTTGIPLSQFQA
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
EHGFADSPYDSLQFCLDLPRPELYQRIDDRVDAMLAAGLVAEVQGLLQSGVPADCKPLQA
HCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IGYKEVLAHLQGEYDHNEMVRLIKRNTRHFAKRQLTWFRSEPAMQWVAYPENSATIHSAA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
ATFFA
HHHCC
>Mature Secondary Structure
MKPRVLVIAGPTASGKSALALDLAVALDGEIICADSLTVYRGLDIGSAKPTAEQQRQIPH
CCCCEEEEECCCCCCCCHHHEEEEEEECCCEEEECCEEHHCCCCCCCCCCCHHHHHCCHH
HLLDIREPTEPFTAADFKLAAKAAIAAICQRGKRPILAGGTGLYLRALLRGLNDAPGEDP
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCCH
VLRETLRKRLECEGAETLLAELAKVDPDTSQRLHPNNRNRIIRALEVFQTTGIPLSQFQA
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
EHGFADSPYDSLQFCLDLPRPELYQRIDDRVDAMLAAGLVAEVQGLLQSGVPADCKPLQA
HCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IGYKEVLAHLQGEYDHNEMVRLIKRNTRHFAKRQLTWFRSEPAMQWVAYPENSATIHSAA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
ATFFA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA