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The map label for this gene is folD [H]

Identifier: 189423579

GI number: 189423579

Start: 482698

End: 483552

Strand: Reverse

Name: folD [H]

Synonym: Glov_0508

Alternate gene names: 189423579

Gene position: 483552-482698 (Counterclockwise)

Preceding gene: 189423580

Following gene: 189423578

Centisome position: 12.34

GC content: 59.77

Gene sequence:

>855_bases
ATGGCAGCACAGATTATTGACGGTAAGGCGATTGCGGCAAAGGTTCGTGAACGGATCGGCAGCACGGTTTCAACCCTGAA
ACAGCAGGGGATCACCCCCGGTCTGGCAGTGGTGCTGGTGGGTGATGATCCGGCCAGCCGGGTCTATGTCGGCATGAAGA
AAAAAATGTGTGTGGAACTGGGGATGTACTCGGCTGACCATGAACTGCCGGCCTCCACCAGCGAGGCAGAGCTGCTGACC
CTGATCGGACAGCTGAATGCCGATCCCCGGGTACACGGTATTCTGGTGCAGTTGCCACTGCCTGACCATATCGATACAGA
CAAGGTGCTGGAGGCGATCTCGCCGGACAAGGATGCTGACGGCTTCCATCCCTACAACGTGGGGCGCCTGGCCATCGGCA
AACCGACCTTCCAGCCCTGCACCCCCTATGGCGTAATGGTGATGCTGGATGAGATCGGCTATGACCTGAAGGGCAAAGAG
GTGGTCGTTGTGGGACGCTCCAACATCGTGGGCAAGCCGGTGGCACTGATGTGCCTGTCCCGTCATGCCACCGTCACCAT
CTGTCACTCCCGCACCAAGGATCTGGCAGATGTGGTCCGCCGGGCGGATGTGGTCATTGCAGCGGTGGGCAAGGCCGAGA
TGGTCAAAGGCGACTGGATCAAGCCGGGGGCCGTGGTGATTGATGTCGGCATCAACCGGGTAGGTGAGAAAAAACTGGTG
GGGGATGTGGAGTATGCTGCGGCTGCCGAACGGGCCAGCGCGATCACCCCGGTACCGGGCGGTGTTGGCCCGATGACCAT
TGCCATGCTGCTGCAAAATACGCTTGAATCTGCCCAACGGGCTGCAACGAGGTAA

Upstream 100 bases:

>100_bases
ATGTTGGGATATTTTAAGACTCGTTCTGATTCCCGTACAAGCTGCCGGCCAACGCAGCCGACAGCTTGTACTGGAATGGG
AATCACACTGGAGGGCAGCC

Downstream 100 bases:

>100_bases
TCCATGAAAGTCCGCAAGGCGATCTTTCCGGTGGCCGGCCTGGGAACCCGCTTCCTGCCTGCCACCAAATCATCTCCCAA
AGAGATGCTACCGCTGATCG

Product: Methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MAAQIIDGKAIAAKVRERIGSTVSTLKQQGITPGLAVVLVGDDPASRVYVGMKKKMCVELGMYSADHELPASTSEAELLT
LIGQLNADPRVHGILVQLPLPDHIDTDKVLEAISPDKDADGFHPYNVGRLAIGKPTFQPCTPYGVMVMLDEIGYDLKGKE
VVVVGRSNIVGKPVALMCLSRHATVTICHSRTKDLADVVRRADVVIAAVGKAEMVKGDWIKPGAVVIDVGINRVGEKKLV
GDVEYAAAAERASAITPVPGGVGPMTIAMLLQNTLESAQRAATR

Sequences:

>Translated_284_residues
MAAQIIDGKAIAAKVRERIGSTVSTLKQQGITPGLAVVLVGDDPASRVYVGMKKKMCVELGMYSADHELPASTSEAELLT
LIGQLNADPRVHGILVQLPLPDHIDTDKVLEAISPDKDADGFHPYNVGRLAIGKPTFQPCTPYGVMVMLDEIGYDLKGKE
VVVVGRSNIVGKPVALMCLSRHATVTICHSRTKDLADVVRRADVVIAAVGKAEMVKGDWIKPGAVVIDVGINRVGEKKLV
GDVEYAAAAERASAITPVPGGVGPMTIAMLLQNTLESAQRAATR
>Mature_283_residues
AAQIIDGKAIAAKVRERIGSTVSTLKQQGITPGLAVVLVGDDPASRVYVGMKKKMCVELGMYSADHELPASTSEAELLTL
IGQLNADPRVHGILVQLPLPDHIDTDKVLEAISPDKDADGFHPYNVGRLAIGKPTFQPCTPYGVMVMLDEIGYDLKGKEV
VVVGRSNIVGKPVALMCLSRHATVTICHSRTKDLADVVRRADVVIAAVGKAEMVKGDWIKPGAVVIDVGINRVGEKKLVG
DVEYAAAAERASAITPVPGGVGPMTIAMLLQNTLESAQRAATR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=48.6206896551724, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=43.8775510204082, Blast_Score=215, Evalue=3e-56,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=44.2176870748299, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI36796743, Length=287, Percent_Identity=28.2229965156794, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=55.5555555555556, Blast_Score=300, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=44.3686006825939, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=48.0836236933798, Blast_Score=265, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=45.3020134228188, Blast_Score=258, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6322933, Length=315, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645718, Length=293, Percent_Identity=48.8054607508532, Blast_Score=270, Evalue=6e-73,
Organism=Drosophila melanogaster, GI17137370, Length=293, Percent_Identity=48.8054607508532, Blast_Score=270, Evalue=6e-73,
Organism=Drosophila melanogaster, GI62472483, Length=293, Percent_Identity=48.8054607508532, Blast_Score=270, Evalue=6e-73,
Organism=Drosophila melanogaster, GI45551871, Length=293, Percent_Identity=48.8054607508532, Blast_Score=270, Evalue=6e-73,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=47.2789115646259, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=47.2789115646259, Blast_Score=250, Evalue=6e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30075; Mature: 29944

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQIIDGKAIAAKVRERIGSTVSTLKQQGITPGLAVVLVGDDPASRVYVGMKKKMCVEL
CCCEEECCHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCEEEECHHHHHHHHH
GMYSADHELPASTSEAELLTLIGQLNADPRVHGILVQLPLPDHIDTDKVLEAISPDKDAD
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHPYNVGRLAIGKPTFQPCTPYGVMVMLDEIGYDLKGKEVVVVGRSNIVGKPVALMCLS
CCCCCCCCEEEECCCCCCCCCCCHHEEEEEHHCCCCCCCEEEEECCCCCCCCCHHHHHHC
RHATVTICHSRTKDLADVVRRADVVIAAVGKAEMVKGDWIKPGAVVIDVGINRVGEKKLV
CCCEEEEECCCCHHHHHHHHHHCEEEEECCCHHCCCCCCCCCCCEEEEECCCCCCCHHHH
GDVEYAAAAERASAITPVPGGVGPMTIAMLLQNTLESAQRAATR
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAQIIDGKAIAAKVRERIGSTVSTLKQQGITPGLAVVLVGDDPASRVYVGMKKKMCVEL
CCEEECCHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCEEEECHHHHHHHHH
GMYSADHELPASTSEAELLTLIGQLNADPRVHGILVQLPLPDHIDTDKVLEAISPDKDAD
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHPYNVGRLAIGKPTFQPCTPYGVMVMLDEIGYDLKGKEVVVVGRSNIVGKPVALMCLS
CCCCCCCCEEEECCCCCCCCCCCHHEEEEEHHCCCCCCCEEEEECCCCCCCCCHHHHHHC
RHATVTICHSRTKDLADVVRRADVVIAAVGKAEMVKGDWIKPGAVVIDVGINRVGEKKLV
CCCEEEEECCCCHHHHHHHHHHCEEEEECCCHHCCCCCCCCCCCEEEEECCCCCCCHHHH
GDVEYAAAAERASAITPVPGGVGPMTIAMLLQNTLESAQRAATR
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA