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The map label for this gene is pcrA [H]

Identifier: 189423552

GI number: 189423552

Start: 456776

End: 458986

Strand: Reverse

Name: pcrA [H]

Synonym: Glov_0481

Alternate gene names: 189423552

Gene position: 458986-456776 (Counterclockwise)

Preceding gene: 189423553

Following gene: 189423551

Centisome position: 11.72

GC content: 59.34

Gene sequence:

>2211_bases
ATGGATTTATTAGCCCACCTCAATACACCGCAGAAACAGGCCGTGCTGCATACTGAAGGGCCACTGCTGATCCTGGCCGG
TGCCGGTTCCGGCAAGACCCGCGTGATCACCCACCGGATTGTCCACCTGATCCGGGAGCATGGCATCCGCCCCTGGAACA
TCCTGGCCGTGACCTTTACCAATAAGGCCGCCAAAGAGATGGCCGAACGGGTGCGGCACCTGCTCGGCTCACACGACACG
CCCCTCATCGCCACCTTCCACGCTACCTGCGGCCGGATTCTACGCCAGGATATCCATCAGCTGGGCTATGAGTCATCCTT
TGCCATCTACGATGACAAGGATAGCGAACGGCTGTTGAAGGATCTGATCAAAGAGCTGAATCTGGACGACAAACGCTATC
CGGCAAACGGGATCGGTGCACGGATTGATGACTGCAAGAACCGGGGACTGTTGCCGGAAGAGTTGCCGGCGGGGGATCTC
TGGAACCAGCAGTTCATCAGGATCTATGCTGCCTATCAGGAACGCCTCAAGCGCTGCAACGCCCTGGATTTTGGCGATAT
GATCCTGCAGACCGTGCGGCTGTTCGAGCAGTTTCCCGAGGTTTTATCCCGCTGGCAGCACCGTTTTCAGTGGCTGCTGG
TGGATGAGTATCAGGACACCAACCCGGTGCAGTACCGCCTGATTCGGCTTTTGGCTGGTGAACGTCAGAACCTCTGCGTC
GTGGGGGATGATGACCAGTCAATCTACTCCTGGCGCGGGGCTGATATCCGCAACATCCTGGAGTTTGAAAAGGATTTTCC
CCAGGTCACGGTCATCCGCCTGGAGCAGAATTACCGCTCCACCCCCACCATCCTGCAGGCGGCCGGGGCAGTGGTGGCCA
GAAATTTTGGACGCAAGGCCAAGACGCTCTGGACGGACAACCCGGATGGTGAACCGATCCGCTGCGAGCGGCTGGAATCC
GACCGGGCCGAGGCACGTTTTGTCTCATCAGAGATCGCCCTGTTACAACGTAACGGGCTGTTGTTGTCGGAGATGGCCGC
CTTTTACCGCACCAATGCCCAATCCCGCCTGTTGGAAGAGGCGCTGGCCGCCGAAGGGATTCCATACCATATCGTGGGCG
GTGTCCGGTTCTATGCCCGGCTTGAGATCAAGGATACCCTGGCCTACCTGCGCCTGTTGGAGAATCCGTCCGATGACGTC
TCGCTGAAGCGGGTCATCAATGTGCCGCCCCGCGGGATCGGGGCTGCCACAGTGGAACGGGTGGCGGAACTGGCCAGACA
GGGACAGATCACCCTGTTTGAGGCCTTTGAGCGGGCAGTGGATTCAAACCTGCTGGGTTCCGGCCCCCGCAGCAAAGTGG
CCGCCTTTGTGGAGCTGCTGCAACGTTTCCGCCGTCTGGCCGCAACCCAGCTGATGCCGGAGCTGGTACGGGCAGTGCTT
GAAGAATCCGGTTATCTGGCACGCTTAAAGGAAAGCCGGGATGAAGAGGACAGCGAACGGCTGGAAAACCTGGCACAGCT
GGTCTCGGCAGTGGAGGAATTCAGCACAGCCAACCCCGAGGCCGGTCTGGCCGACTTTCTGGAACAGGTGGCCCTGGTCT
CTGATCTGGAGCGGGGCGAGGCGGGCAGGCCCTCGGTCACTCTGATGACCCTGCATGCTGCCAAGGGCCTGGAGTACAAG
GCGGTCTTCATGGTAGGGATGGAAGAGCGGCTCTTTCCCCATGTCCGTTCACTGGATGACCCGGACGGCATGGAAGAAGA
GCGCAGGCTCTGCTACGTGGGGATGACCAGGGCACGGGAACGACTCTATCTGCTGTATGCCCGGCGCAGGATGATCTTTG
GCCAGGAACAGGCCAATCTGCCTTCACGCTTTTTAAAGGAGATCCCGGCAGAGCTGATGGAGCGCAATCATGTCTCCTGC
AACCCTGAACTGCCCTCATCAGCACAGCCTAAAAGCTCTTTTTCTCCTGCCCCCTCCCACAACCTTGCTGCAGTGGCTTC
AGCCATGGCCGAGGTAGAGGTGGTGCCGGAACCTGGCGATGACTATGGCAGTGGTATCTATGTCGGGCTCAAGGTGCGCC
ATGCCAAATTCGGGGTGGGCACCATCCGAAAGCTAGAAGGCAAGGGTGATGACCAGAAGGCAATCGTCTGGTTTGCCGGT
TGCGGCCCGAAAAAACTGCTGCTGAGATTTGCCGGACTGGAGCGGGCGTAG

Upstream 100 bases:

>100_bases
CCGGAAGACTTCAGCGGGATCCTGGCCTATCTGCGGGAGACCGCTGCCGTAACCACTACTTTTTCTTGACGCTGCAGATA
CACGATACTACAACCCCTGT

Downstream 100 bases:

>100_bases
ACCATGGAATCAATAACTCTCAACTATGAGCTGGTGCTGGAAGCCCAGGATCGTCTGCGCAAACGGGTTCGCCATACAGA
GCTGATCAGCTCCAATCACT

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MDLLAHLNTPQKQAVLHTEGPLLILAGAGSGKTRVITHRIVHLIREHGIRPWNILAVTFTNKAAKEMAERVRHLLGSHDT
PLIATFHATCGRILRQDIHQLGYESSFAIYDDKDSERLLKDLIKELNLDDKRYPANGIGARIDDCKNRGLLPEELPAGDL
WNQQFIRIYAAYQERLKRCNALDFGDMILQTVRLFEQFPEVLSRWQHRFQWLLVDEYQDTNPVQYRLIRLLAGERQNLCV
VGDDDQSIYSWRGADIRNILEFEKDFPQVTVIRLEQNYRSTPTILQAAGAVVARNFGRKAKTLWTDNPDGEPIRCERLES
DRAEARFVSSEIALLQRNGLLLSEMAAFYRTNAQSRLLEEALAAEGIPYHIVGGVRFYARLEIKDTLAYLRLLENPSDDV
SLKRVINVPPRGIGAATVERVAELARQGQITLFEAFERAVDSNLLGSGPRSKVAAFVELLQRFRRLAATQLMPELVRAVL
EESGYLARLKESRDEEDSERLENLAQLVSAVEEFSTANPEAGLADFLEQVALVSDLERGEAGRPSVTLMTLHAAKGLEYK
AVFMVGMEERLFPHVRSLDDPDGMEEERRLCYVGMTRARERLYLLYARRRMIFGQEQANLPSRFLKEIPAELMERNHVSC
NPELPSSAQPKSSFSPAPSHNLAAVASAMAEVEVVPEPGDDYGSGIYVGLKVRHAKFGVGTIRKLEGKGDDQKAIVWFAG
CGPKKLLLRFAGLERA

Sequences:

>Translated_736_residues
MDLLAHLNTPQKQAVLHTEGPLLILAGAGSGKTRVITHRIVHLIREHGIRPWNILAVTFTNKAAKEMAERVRHLLGSHDT
PLIATFHATCGRILRQDIHQLGYESSFAIYDDKDSERLLKDLIKELNLDDKRYPANGIGARIDDCKNRGLLPEELPAGDL
WNQQFIRIYAAYQERLKRCNALDFGDMILQTVRLFEQFPEVLSRWQHRFQWLLVDEYQDTNPVQYRLIRLLAGERQNLCV
VGDDDQSIYSWRGADIRNILEFEKDFPQVTVIRLEQNYRSTPTILQAAGAVVARNFGRKAKTLWTDNPDGEPIRCERLES
DRAEARFVSSEIALLQRNGLLLSEMAAFYRTNAQSRLLEEALAAEGIPYHIVGGVRFYARLEIKDTLAYLRLLENPSDDV
SLKRVINVPPRGIGAATVERVAELARQGQITLFEAFERAVDSNLLGSGPRSKVAAFVELLQRFRRLAATQLMPELVRAVL
EESGYLARLKESRDEEDSERLENLAQLVSAVEEFSTANPEAGLADFLEQVALVSDLERGEAGRPSVTLMTLHAAKGLEYK
AVFMVGMEERLFPHVRSLDDPDGMEEERRLCYVGMTRARERLYLLYARRRMIFGQEQANLPSRFLKEIPAELMERNHVSC
NPELPSSAQPKSSFSPAPSHNLAAVASAMAEVEVVPEPGDDYGSGIYVGLKVRHAKFGVGTIRKLEGKGDDQKAIVWFAG
CGPKKLLLRFAGLERA
>Mature_736_residues
MDLLAHLNTPQKQAVLHTEGPLLILAGAGSGKTRVITHRIVHLIREHGIRPWNILAVTFTNKAAKEMAERVRHLLGSHDT
PLIATFHATCGRILRQDIHQLGYESSFAIYDDKDSERLLKDLIKELNLDDKRYPANGIGARIDDCKNRGLLPEELPAGDL
WNQQFIRIYAAYQERLKRCNALDFGDMILQTVRLFEQFPEVLSRWQHRFQWLLVDEYQDTNPVQYRLIRLLAGERQNLCV
VGDDDQSIYSWRGADIRNILEFEKDFPQVTVIRLEQNYRSTPTILQAAGAVVARNFGRKAKTLWTDNPDGEPIRCERLES
DRAEARFVSSEIALLQRNGLLLSEMAAFYRTNAQSRLLEEALAAEGIPYHIVGGVRFYARLEIKDTLAYLRLLENPSDDV
SLKRVINVPPRGIGAATVERVAELARQGQITLFEAFERAVDSNLLGSGPRSKVAAFVELLQRFRRLAATQLMPELVRAVL
EESGYLARLKESRDEEDSERLENLAQLVSAVEEFSTANPEAGLADFLEQVALVSDLERGEAGRPSVTLMTLHAAKGLEYK
AVFMVGMEERLFPHVRSLDDPDGMEEERRLCYVGMTRARERLYLLYARRRMIFGQEQANLPSRFLKEIPAELMERNHVSC
NPELPSSAQPKSSFSPAPSHNLAAVASAMAEVEVVPEPGDDYGSGIYVGLKVRHAKFGVGTIRKLEGKGDDQKAIVWFAG
CGPKKLLLRFAGLERA

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=737, Percent_Identity=41.7910447761194, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI48994965, Length=638, Percent_Identity=41.3793103448276, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1787196, Length=348, Percent_Identity=27.8735632183908, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=754, Percent_Identity=28.1167108753316, Blast_Score=227, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6324477, Length=670, Percent_Identity=22.8358208955224, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83004; Mature: 83004

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLAHLNTPQKQAVLHTEGPLLILAGAGSGKTRVITHRIVHLIREHGIRPWNILAVTFT
CCCCCCCCCCHHHEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEC
NKAAKEMAERVRHLLGSHDTPLIATFHATCGRILRQDIHQLGYESSFAIYDDKDSERLLK
CHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHH
DLIKELNLDDKRYPANGIGARIDDCKNRGLLPEELPAGDLWNQQFIRIYAAYQERLKRCN
HHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ALDFGDMILQTVRLFEQFPEVLSRWQHRFQWLLVDEYQDTNPVQYRLIRLLAGERQNLCV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
VGDDDQSIYSWRGADIRNILEFEKDFPQVTVIRLEQNYRSTPTILQAAGAVVARNFGRKA
ECCCCCCHHEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHH
KTLWTDNPDGEPIRCERLESDRAEARFVSSEIALLQRNGLLLSEMAAFYRTNAQSRLLEE
CEEECCCCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
ALAAEGIPYHIVGGVRFYARLEIKDTLAYLRLLENPSDDVSLKRVINVPPRGIGAATVER
HHHHCCCCEEEECCEEEEEEEEHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHH
VAELARQGQITLFEAFERAVDSNLLGSGPRSKVAAFVELLQRFRRLAATQLMPELVRAVL
HHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EESGYLARLKESRDEEDSERLENLAQLVSAVEEFSTANPEAGLADFLEQVALVSDLERGE
HCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
AGRPSVTLMTLHAAKGLEYKAVFMVGMEERLFPHVRSLDDPDGMEEERRLCYVGMTRARE
CCCCCEEEEEEEHHCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHEEHHHHHHHHH
RLYLLYARRRMIFGQEQANLPSRFLKEIPAELMERNHVSCNPELPSSAQPKSSFSPAPSH
HHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
NLAAVASAMAEVEVVPEPGDDYGSGIYVGLKVRHAKFGVGTIRKLEGKGDDQKAIVWFAG
HHHHHHHHHHHEEECCCCCCCCCCCEEEEEEEEEEECCCCHHHCCCCCCCCCEEEEEEEC
CGPKKLLLRFAGLERA
CCHHHHHHHHHCCCCC
>Mature Secondary Structure
MDLLAHLNTPQKQAVLHTEGPLLILAGAGSGKTRVITHRIVHLIREHGIRPWNILAVTFT
CCCCCCCCCCHHHEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEC
NKAAKEMAERVRHLLGSHDTPLIATFHATCGRILRQDIHQLGYESSFAIYDDKDSERLLK
CHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHH
DLIKELNLDDKRYPANGIGARIDDCKNRGLLPEELPAGDLWNQQFIRIYAAYQERLKRCN
HHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ALDFGDMILQTVRLFEQFPEVLSRWQHRFQWLLVDEYQDTNPVQYRLIRLLAGERQNLCV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
VGDDDQSIYSWRGADIRNILEFEKDFPQVTVIRLEQNYRSTPTILQAAGAVVARNFGRKA
ECCCCCCHHEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHH
KTLWTDNPDGEPIRCERLESDRAEARFVSSEIALLQRNGLLLSEMAAFYRTNAQSRLLEE
CEEECCCCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
ALAAEGIPYHIVGGVRFYARLEIKDTLAYLRLLENPSDDVSLKRVINVPPRGIGAATVER
HHHHCCCCEEEECCEEEEEEEEHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHH
VAELARQGQITLFEAFERAVDSNLLGSGPRSKVAAFVELLQRFRRLAATQLMPELVRAVL
HHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EESGYLARLKESRDEEDSERLENLAQLVSAVEEFSTANPEAGLADFLEQVALVSDLERGE
HCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
AGRPSVTLMTLHAAKGLEYKAVFMVGMEERLFPHVRSLDDPDGMEEERRLCYVGMTRARE
CCCCCEEEEEEEHHCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHEEHHHHHHHHH
RLYLLYARRRMIFGQEQANLPSRFLKEIPAELMERNHVSCNPELPSSAQPKSSFSPAPSH
HHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
NLAAVASAMAEVEVVPEPGDDYGSGIYVGLKVRHAKFGVGTIRKLEGKGDDQKAIVWFAG
HHHHHHHHHHHEEECCCCCCCCCCCEEEEEEEEEEECCCCHHHCCCCCCCCCEEEEEEEC
CGPKKLLLRFAGLERA
CCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA