Click here to switch to the map view.

The map label for this gene is truA [H]

Identifier: 189423417

GI number: 189423417

Start: 317317

End: 318105

Strand: Reverse

Name: truA [H]

Synonym: Glov_0346

Alternate gene names: 189423417

Gene position: 318105-317317 (Counterclockwise)

Preceding gene: 189423418

Following gene: 189423416

Centisome position: 8.12

GC content: 58.43

Gene sequence:

>789_bases
ATGAGAAGCCTGACACCGAGATTGCGGGAAAAGACCGCTCCCTTTTTTATGCGAACCATAAAGCTTATTATTCAATACGA
CGGCACCAACTACTGCGGCTGGCAGGAACAGACCAACGGCCCTTCGATCCAGGAGACCGTGGAACTGGCACTGGCCAAAA
TCCTCGGGCAGCGGGTACGGGTGCAGTCATCCGGCCGTACCGATGCCGGGGTACATGCCGTTGCCATGCCGGCTGTGTTC
CGTACCGAATCAACAATTCCTCTCAAGGCATTTGTAGATGGCGTTAACTGCCACCTGCCCGACGATATTGCAATTCAGTC
TGCCGTGGAAGTACCAACCGGCTTTCGCGCCATTGGGGGAGCCCGTTCCAAAACCTACCGCTACACCATCTATAACGCCC
CTGTCCGCAGTCCGCTCAACCGTCGCACCTCCTGGCATGTTCGCGAACAGCTTGACCTGCAGGCCATGCAGCAGGCAGCC
GCATATTTCGTCGGCGAACATGACTTTGCCGCATTTCGTGGCGCCAATTGCAGCGCAGTAACCAGCCGACGCAGGATTGA
TGCAGTGCAGATCAATCAGAGCGGCCCCCTTATCACCATTGATGTTACCGGTGGCGGTTTCCTGAAGTACATGGTGCGGA
TCATGGCCGGCACTCTGGTGGATGTGGGGCGCGGACGTTTTGCGCCGGAGCATGTTGCCGGCCTGCTGGCAGAACCGGAC
CGCCAGCGCGGCGGAGTCACCGCCCCTCCCCAGGGGTTGTCGTTGCTGCAGGTAATTTATCCGGGCTGA

Upstream 100 bases:

>100_bases
CTCAATTGACGAGGAGTTCAGGGAGCGAGGATTTTTTCGCAAGGTCAAGGAAGGCGAGCATTCACGCGGAGGCGTGCAGA
ACAGTACGCCACACAAGGGA

Downstream 100 bases:

>100_bases
CAACCGTCATGCGAAAAACCGAATAAATTGCCTTGACTCTGGGAACTCGCTTAAGTAAAATCCGGTTCTACTTTAGCGTA
AGTCAGTTAAGTTTTTTTCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRSLTPRLREKTAPFFMRTIKLIIQYDGTNYCGWQEQTNGPSIQETVELALAKILGQRVRVQSSGRTDAGVHAVAMPAVF
RTESTIPLKAFVDGVNCHLPDDIAIQSAVEVPTGFRAIGGARSKTYRYTIYNAPVRSPLNRRTSWHVREQLDLQAMQQAA
AYFVGEHDFAAFRGANCSAVTSRRRIDAVQINQSGPLITIDVTGGGFLKYMVRIMAGTLVDVGRGRFAPEHVAGLLAEPD
RQRGGVTAPPQGLSLLQVIYPG

Sequences:

>Translated_262_residues
MRSLTPRLREKTAPFFMRTIKLIIQYDGTNYCGWQEQTNGPSIQETVELALAKILGQRVRVQSSGRTDAGVHAVAMPAVF
RTESTIPLKAFVDGVNCHLPDDIAIQSAVEVPTGFRAIGGARSKTYRYTIYNAPVRSPLNRRTSWHVREQLDLQAMQQAA
AYFVGEHDFAAFRGANCSAVTSRRRIDAVQINQSGPLITIDVTGGGFLKYMVRIMAGTLVDVGRGRFAPEHVAGLLAEPD
RQRGGVTAPPQGLSLLQVIYPG
>Mature_262_residues
MRSLTPRLREKTAPFFMRTIKLIIQYDGTNYCGWQEQTNGPSIQETVELALAKILGQRVRVQSSGRTDAGVHAVAMPAVF
RTESTIPLKAFVDGVNCHLPDDIAIQSAVEVPTGFRAIGGARSKTYRYTIYNAPVRSPLNRRTSWHVREQLDLQAMQQAA
AYFVGEHDFAAFRGANCSAVTSRRRIDAVQINQSGPLITIDVTGGGFLKYMVRIMAGTLVDVGRGRFAPEHVAGLLAEPD
RQRGGVTAPPQGLSLLQVIYPG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=30.7407407407407, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=37.1900826446281, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28712; Mature: 28712

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLTPRLREKTAPFFMRTIKLIIQYDGTNYCGWQEQTNGPSIQETVELALAKILGQRVR
CCCCCHHHHHHCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEE
VQSSGRTDAGVHAVAMPAVFRTESTIPLKAFVDGVNCHLPDDIAIQSAVEVPTGFRAIGG
ECCCCCCCCCEEHEEHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHCC
ARSKTYRYTIYNAPVRSPLNRRTSWHVREQLDLQAMQQAAAYFVGEHDFAAFRGANCSAV
CCCCEEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHH
TSRRRIDAVQINQSGPLITIDVTGGGFLKYMVRIMAGTLVDVGRGRFAPEHVAGLLAEPD
HHHHCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
RQRGGVTAPPQGLSLLQVIYPG
HHCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MRSLTPRLREKTAPFFMRTIKLIIQYDGTNYCGWQEQTNGPSIQETVELALAKILGQRVR
CCCCCHHHHHHCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEE
VQSSGRTDAGVHAVAMPAVFRTESTIPLKAFVDGVNCHLPDDIAIQSAVEVPTGFRAIGG
ECCCCCCCCCEEHEEHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHCC
ARSKTYRYTIYNAPVRSPLNRRTSWHVREQLDLQAMQQAAAYFVGEHDFAAFRGANCSAV
CCCCEEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHH
TSRRRIDAVQINQSGPLITIDVTGGGFLKYMVRIMAGTLVDVGRGRFAPEHVAGLLAEPD
HHHHCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
RQRGGVTAPPQGLSLLQVIYPG
HHCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA