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The map label for this gene is vanB [H]

Identifier: 189354059

GI number: 189354059

Start: 2389554

End: 2390588

Strand: Direct

Name: vanB [H]

Synonym: BMULJ_05312

Alternate gene names: 189354059

Gene position: 2389554-2390588 (Clockwise)

Preceding gene: 189354058

Following gene: 189354071

Centisome position: 96.62

GC content: 69.76

Gene sequence:

>1035_bases
ATGAAAGTCACGCTTCTCCGCAAGCTTCCCGTCGCGACCGACATCTGCATGTTCGAACTCGGGCCGGCCGACGGCGCCGC
GCTGCCGCCGTTCACGGCCGGCGCGCATATCGACATCCATCTCGGCGGACTCGTTCGCCAGTATTCGCTGTGCAACAGCC
CGTCGGAGGCGGGCGTCTACCGGCTCGGCGTGCTGCGCGAGCGCGAATCGCGCGGCGGCTCGGTCGCGATGCATGCATGC
GAGCCCGGCACGACGCTCGAGATCGGCGAGCCGCGCAATCACTTCCCGCTCGATCCGCATGCCGCGCACAGCGTGCTGCT
CGCCGGCGGCATCGGCATCACGCCGCTGCTCAGCATGGCGCTGCATCTGGCCGAGACCGCGCGATCGTTCGAACTGCACT
ACTGCGCGCGCGAACCGGCGCGCGCGGCGTTCGTCGACCGGCTCGATACGCCGCCGCTGAACGTGCGCACGCGGCTGTGG
TTCGACGATGCACCTGCCGGGCAGCGCATCGATTTCGCGCAGGTACTCGGCAAGCCCGATGCGCACACGCATCTGTACGT
GTGCGGGCCGGCCGGGTTCATCGCGGCGGCGCTCGGCGCGGCGGCGCAGGCCGGGTGGGATCCGGCGAATGTGCATCGGG
AGTACTTTGGCGCTGCCGGCTTAGGTGCGGCCGGCGTAGGTGCGGCCGGCTTAGGCGCTGCCGGCGTAGGTGCGACCGGC
GCAGGCGCTGCCGGCGAAAGCGAAACATCGACAGCAGCAACAGCCGATGGTCCGTTCCAGGTATCGCTCGCGCAATCGGG
CCGCGTCGTCGACGTGCCGGCCGGTACGACGATCGTCGAAGCGCTGCGCGGCTGCGGGATCGAGGTGCCCGTGTCGTGCG
AACAAGGCGTGTGCGGCACGTGCCTCACGCGCGTGCTGTCCGGCACGCCCGATCATCGCGACGTCTACCTGACCGACGAC
GAACGCGCGGCGAACGATCAGATGCTGCCGTGCTGTTCGCGCGCGCGCACGCCGATGCTCGTGCTCGATCTCTGA

Upstream 100 bases:

>100_bases
GACCGGCTGATCGAACAGGAGCGCGCTGCCGCGCTATCGTGCTGACCCTCGCGCCGCGTCCGCGCGCACGCGCGGCGCGG
CGCCCTCATTGACCGTTGCC

Downstream 100 bases:

>100_bases
GAGGGCCGCGCGGCGCGAGCCGCGCAGGCCGAAGCGCGGCCGCTACGTTTCGCGGCAAACGCCGCGCGCCGACACCGCGC
GTTGCACGCATGGCGACATC

Product: vanillate monooxygenase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKVTLLRKLPVATDICMFELGPADGAALPPFTAGAHIDIHLGGLVRQYSLCNSPSEAGVYRLGVLRERESRGGSVAMHAC
EPGTTLEIGEPRNHFPLDPHAAHSVLLAGGIGITPLLSMALHLAETARSFELHYCAREPARAAFVDRLDTPPLNVRTRLW
FDDAPAGQRIDFAQVLGKPDAHTHLYVCGPAGFIAAALGAAAQAGWDPANVHREYFGAAGLGAAGVGAAGLGAAGVGATG
AGAAGESETSTAATADGPFQVSLAQSGRVVDVPAGTTIVEALRGCGIEVPVSCEQGVCGTCLTRVLSGTPDHRDVYLTDD
ERAANDQMLPCCSRARTPMLVLDL

Sequences:

>Translated_344_residues
MKVTLLRKLPVATDICMFELGPADGAALPPFTAGAHIDIHLGGLVRQYSLCNSPSEAGVYRLGVLRERESRGGSVAMHAC
EPGTTLEIGEPRNHFPLDPHAAHSVLLAGGIGITPLLSMALHLAETARSFELHYCAREPARAAFVDRLDTPPLNVRTRLW
FDDAPAGQRIDFAQVLGKPDAHTHLYVCGPAGFIAAALGAAAQAGWDPANVHREYFGAAGLGAAGVGAAGLGAAGVGATG
AGAAGESETSTAATADGPFQVSLAQSGRVVDVPAGTTIVEALRGCGIEVPVSCEQGVCGTCLTRVLSGTPDHRDVYLTDD
ERAANDQMLPCCSRARTPMLVLDL
>Mature_344_residues
MKVTLLRKLPVATDICMFELGPADGAALPPFTAGAHIDIHLGGLVRQYSLCNSPSEAGVYRLGVLRERESRGGSVAMHAC
EPGTTLEIGEPRNHFPLDPHAAHSVLLAGGIGITPLLSMALHLAETARSFELHYCAREPARAAFVDRLDTPPLNVRTRLW
FDDAPAGQRIDFAQVLGKPDAHTHLYVCGPAGFIAAALGAAAQAGWDPANVHREYFGAAGLGAAGVGAAGLGAAGVGATG
AGAAGESETSTAATADGPFQVSLAQSGRVVDVPAGTTIVEALRGCGIEVPVSCEQGVCGTCLTRVLSGTPDHRDVYLTDD
ERAANDQMLPCCSRARTPMLVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=350, Percent_Identity=34.5714285714286, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1788903, Length=238, Percent_Identity=25.2100840336134, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 35603; Mature: 35603

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTLLRKLPVATDICMFELGPADGAALPPFTAGAHIDIHLGGLVRQYSLCNSPSEAGVY
CEEEEEECCCCHHCEEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCEE
RLGVLRERESRGGSVAMHACEPGTTLEIGEPRNHFPLDPHAAHSVLLAGGIGITPLLSMA
EEHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCCCCHHHHEEEEECCCCHHHHHHHH
LHLAETARSFELHYCAREPARAAFVDRLDTPPLNVRTRLWFDDAPAGQRIDFAQVLGKPD
HHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCC
AHTHLYVCGPAGFIAAALGAAAQAGWDPANVHREYFGAAGLGAAGVGAAGLGAAGVGATG
CCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
AGAAGESETSTAATADGPFQVSLAQSGRVVDVPAGTTIVEALRGCGIEVPVSCEQGVCGT
CCCCCCCCCCCEECCCCCEEEEEECCCCEEECCCCHHHHHHHHHCCCEECCCCCCCCHHH
CLTRVLSGTPDHRDVYLTDDERAANDQMLPCCSRARTPMLVLDL
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCEEEEEC
>Mature Secondary Structure
MKVTLLRKLPVATDICMFELGPADGAALPPFTAGAHIDIHLGGLVRQYSLCNSPSEAGVY
CEEEEEECCCCHHCEEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCEE
RLGVLRERESRGGSVAMHACEPGTTLEIGEPRNHFPLDPHAAHSVLLAGGIGITPLLSMA
EEHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCCCCHHHHEEEEECCCCHHHHHHHH
LHLAETARSFELHYCAREPARAAFVDRLDTPPLNVRTRLWFDDAPAGQRIDFAQVLGKPD
HHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCC
AHTHLYVCGPAGFIAAALGAAAQAGWDPANVHREYFGAAGLGAAGVGAAGLGAAGVGATG
CCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
AGAAGESETSTAATADGPFQVSLAQSGRVVDVPAGTTIVEALRGCGIEVPVSCEQGVCGT
CCCCCCCCCCCEECCCCCEEEEEECCCCEEECCCCHHHHHHHHHCCCEECCCCCCCCHHH
CLTRVLSGTPDHRDVYLTDDERAANDQMLPCCSRARTPMLVLDL
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579070 [H]