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The map label for this gene is pgl [H]

Identifier: 189354051

GI number: 189354051

Start: 2378602

End: 2380599

Strand: Reverse

Name: pgl [H]

Synonym: BMULJ_05304

Alternate gene names: 189354051

Gene position: 2380599-2378602 (Counterclockwise)

Preceding gene: 189354053

Following gene: 189354048

Centisome position: 96.26

GC content: 68.42

Gene sequence:

>1998_bases
ATGGCCGCTGTCCGCCGCCGTCCGTCTTCGTCCGTTCGTTCGCGCTCGCCGAAGCGCCTGTCGCGCCGCGCCTTCCTCGG
CTGGACCGGCGCACTGGCCGGCGGCACGGTGCTCGGCGGCCCGCTCGCGGCCACGCGCGCCTGGGCCGGCAAGCTGCCCG
CGTCGTCCGCGGTCGACCTGATCTGGGGCGAGCACGGCGCGGCCGCACGAATTGCCGCGTCGCTCGCGCACGTGTCGCGG
CTTGCATTCCGCGCGCGCGACTTCGACGTCACGCATTACGGCGCGCGTCCGTGCGCGACGGTCCCGCAAACGTCGCCGTA
CACGAACGCCGCGAAATCGCCGGTGAGCCCCGGTGCCGACCGGACGACGGCGCCCGGCGCCTTCGACTCGCGTCCGGCGT
TTCTCGCCGCGATCGACGCGTGCGTGCGCGAAGGCGGCGGTCGAGTCGTCGTGCCGCCCGGCAACTGGTATTGCGCGGGC
CCGATCGTGCTGCAGAGCAACGTGAACTTCCATCTGAGCGCAAACTGCACGATCTATTTCAGCCCGAACCCGGCCGACTA
CGCGAAGGACGGCCCCGTCGACTGCGGCGCGAACGGTCGGCTGTACTACAGCCGCTGGCAGGCGAACGACTGCCTGAACT
ACGGCGCGCCCGTGTACGCGCGCAACGCGGTGAACATCGCGCTGACCGGCGAAGGGCCGACGTCCGTGCTGAACGGGCAG
GCGATGACGCCGTTTGCCGGCAGCGGCAACGACAGCGTGTGCTGGTGGACGTACAAGGGCTCGTCGGGCGCGTACGGCGC
GGCGTCGTCGGTGCCGAGCCAGGCGTATCCGAATCCGAACAACCTCGATCTGCGCATCGTCGCGCCCGGCATCTCCGATG
CGCTGTACGCGATGCTGACGTCGCCCGTCACGCCGTGGCAGCAGGACCAGAACTATCTGCCCGCGCTCTCCGAAGCCGGC
GTGCCGGTCGACAAGCGCATCTTCGGGCTCGGTCATTATCTGCGGCCGTGCATGGTCGAATTCATCGGCTGCACGAACGT
GCTGATGGAGAACTACCAGACGCAGAACACGCCGTTCTGGCAGCACCATCCGACCGCGTGCCGCAACGTCGTGATCCGCG
GCGTGACGACCAACAGCATCGGCCCGAACAACGACGGCTTCGATCCGGACGCATGCACCGACGTGCTCTGCGAGGACTGC
ACGTTCAACACCGGCGACGACTGCATCGCGATCAAGTCCGGCAAGAACCGCGACACCGAGTACGGGCCGGCGAAGCGGCA
TCTGGTGCGCAACTGCACGATGAACAGCGGCCACGGCGGGATCACGCTCGGCAGCGAGATGGGCGGCGGCGTCGAGCAGA
TCTACGCGACGAACCTGTCGATGCTGAACGCGAACTGGCAGACCAATCCGCTGAACATCGCGATTCGCGTGAAGACGAAC
ATGAATCGCGGCGGCTACGTGAAGGACTTTCACGTGAAGGGCGTGTCGCTGCCGAACGGCGTCACGCTGAAGGGCGGCGG
CTACGGCAGCGCGCTGCTCGCGGGCAGCCCGATCAATGCGAGCGTGCCGCTCGGCGTCGTGACGCCGGCCGCCGGCAATC
CGTCGGCCGCGCAAGGCGGGATCGTCACGTTCGACTGCGACTATCAACCGGCGAACGACGCGGTGCGCACGCGGGCGCCG
GTCGTGCAGAACGTGACGATCGCCGACGTGACCGCGCGCAACGTGACGCTGAACGGCGTGACCGCATCGTGCTTTCAGGC
GATCGTCGCGCAGGGGCCGGTCGCGTTCGACTACAACGGCGCGTCGCCCGCCCCGACCGTGCAACCGATCGCCGGCGTAA
CGATCAGCCACTGCGATTTCGGCACGCCGGTCGCATCGGGCACGCCGACCGTCACGTCGCCGGGGCCGATCTATGCGTTC
AACGTGAGCGGGATGACGCTGCGCAACGTCGTGATTGCCGGGCAGACGGTCAACACGACGATCACCGATTCGCGTTGA

Upstream 100 bases:

>100_bases
TATTCGCGACTAGACTGCTCCAAGTAGTACGACGACGTATGAATAATCGGCGTGCCCGCCCGGCGCGTCGCCCGCCCTTT
ATCGACTTGAAGGAGTGTCC

Downstream 100 bases:

>100_bases
TCGTGGCAGCAAGAAGCGGCGTGCGCGATCTCGCGCTGCGCGCCGCTTGCGCCGATCATGCGAGCCGCTTCACCATGTGG
TGCTCGTCGTAGAACCGACC

Product: endopolygalacturonase

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MAAVRRRPSSSVRSRSPKRLSRRAFLGWTGALAGGTVLGGPLAATRAWAGKLPASSAVDLIWGEHGAAARIAASLAHVSR
LAFRARDFDVTHYGARPCATVPQTSPYTNAAKSPVSPGADRTTAPGAFDSRPAFLAAIDACVREGGGRVVVPPGNWYCAG
PIVLQSNVNFHLSANCTIYFSPNPADYAKDGPVDCGANGRLYYSRWQANDCLNYGAPVYARNAVNIALTGEGPTSVLNGQ
AMTPFAGSGNDSVCWWTYKGSSGAYGAASSVPSQAYPNPNNLDLRIVAPGISDALYAMLTSPVTPWQQDQNYLPALSEAG
VPVDKRIFGLGHYLRPCMVEFIGCTNVLMENYQTQNTPFWQHHPTACRNVVIRGVTTNSIGPNNDGFDPDACTDVLCEDC
TFNTGDDCIAIKSGKNRDTEYGPAKRHLVRNCTMNSGHGGITLGSEMGGGVEQIYATNLSMLNANWQTNPLNIAIRVKTN
MNRGGYVKDFHVKGVSLPNGVTLKGGGYGSALLAGSPINASVPLGVVTPAAGNPSAAQGGIVTFDCDYQPANDAVRTRAP
VVQNVTIADVTARNVTLNGVTASCFQAIVAQGPVAFDYNGASPAPTVQPIAGVTISHCDFGTPVASGTPTVTSPGPIYAF
NVSGMTLRNVVIAGQTVNTTITDSR

Sequences:

>Translated_665_residues
MAAVRRRPSSSVRSRSPKRLSRRAFLGWTGALAGGTVLGGPLAATRAWAGKLPASSAVDLIWGEHGAAARIAASLAHVSR
LAFRARDFDVTHYGARPCATVPQTSPYTNAAKSPVSPGADRTTAPGAFDSRPAFLAAIDACVREGGGRVVVPPGNWYCAG
PIVLQSNVNFHLSANCTIYFSPNPADYAKDGPVDCGANGRLYYSRWQANDCLNYGAPVYARNAVNIALTGEGPTSVLNGQ
AMTPFAGSGNDSVCWWTYKGSSGAYGAASSVPSQAYPNPNNLDLRIVAPGISDALYAMLTSPVTPWQQDQNYLPALSEAG
VPVDKRIFGLGHYLRPCMVEFIGCTNVLMENYQTQNTPFWQHHPTACRNVVIRGVTTNSIGPNNDGFDPDACTDVLCEDC
TFNTGDDCIAIKSGKNRDTEYGPAKRHLVRNCTMNSGHGGITLGSEMGGGVEQIYATNLSMLNANWQTNPLNIAIRVKTN
MNRGGYVKDFHVKGVSLPNGVTLKGGGYGSALLAGSPINASVPLGVVTPAAGNPSAAQGGIVTFDCDYQPANDAVRTRAP
VVQNVTIADVTARNVTLNGVTASCFQAIVAQGPVAFDYNGASPAPTVQPIAGVTISHCDFGTPVASGTPTVTSPGPIYAF
NVSGMTLRNVVIAGQTVNTTITDSR
>Mature_664_residues
AAVRRRPSSSVRSRSPKRLSRRAFLGWTGALAGGTVLGGPLAATRAWAGKLPASSAVDLIWGEHGAAARIAASLAHVSRL
AFRARDFDVTHYGARPCATVPQTSPYTNAAKSPVSPGADRTTAPGAFDSRPAFLAAIDACVREGGGRVVVPPGNWYCAGP
IVLQSNVNFHLSANCTIYFSPNPADYAKDGPVDCGANGRLYYSRWQANDCLNYGAPVYARNAVNIALTGEGPTSVLNGQA
MTPFAGSGNDSVCWWTYKGSSGAYGAASSVPSQAYPNPNNLDLRIVAPGISDALYAMLTSPVTPWQQDQNYLPALSEAGV
PVDKRIFGLGHYLRPCMVEFIGCTNVLMENYQTQNTPFWQHHPTACRNVVIRGVTTNSIGPNNDGFDPDACTDVLCEDCT
FNTGDDCIAIKSGKNRDTEYGPAKRHLVRNCTMNSGHGGITLGSEMGGGVEQIYATNLSMLNANWQTNPLNIAIRVKTNM
NRGGYVKDFHVKGVSLPNGVTLKGGGYGSALLAGSPINASVPLGVVTPAAGNPSAAQGGIVTFDCDYQPANDAVRTRAPV
VQNVTIADVTARNVTLNGVTASCFQAIVAQGPVAFDYNGASPAPTVQPIAGVTISHCDFGTPVASGTPTVTSPGPIYAFN
VSGMTLRNVVIAGQTVNTTITDSR

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 69711; Mature: 69580

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVRRRPSSSVRSRSPKRLSRRAFLGWTGALAGGTVLGGPLAATRAWAGKLPASSAVDL
CCCCCCCCCHHHHCCCHHHHHHHEEEEECHHHCCCCEECCCHHHHHHHHCCCCCCCCEEE
IWGEHGAAARIAASLAHVSRLAFRARDFDVTHYGARPCATVPQTSPYTNAAKSPVSPGAD
EECCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
RTTAPGAFDSRPAFLAAIDACVREGGGRVVVPPGNWYCAGPIVLQSNVNFHLSANCTIYF
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCEEECCCEEEECCCCEEEEEEEEEEE
SPNPADYAKDGPVDCGANGRLYYSRWQANDCLNYGAPVYARNAVNIALTGEGPTSVLNGQ
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCEEECCEEEEEEECCCCCHHCCCC
AMTPFAGSGNDSVCWWTYKGSSGAYGAASSVPSQAYPNPNNLDLRIVAPGISDALYAMLT
EECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
SPVTPWQQDQNYLPALSEAGVPVDKRIFGLGHYLRPCMVEFIGCTNVLMENYQTQNTPFW
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QHHPTACRNVVIRGVTTNSIGPNNDGFDPDACTDVLCEDCTFNTGDDCIAIKSGKNRDTE
CCCCHHHCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCC
YGPAKRHLVRNCTMNSGHGGITLGSEMGGGVEQIYATNLSMLNANWQTNPLNIAIRVKTN
CCHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHCCEEEEECCCCCCCEEEEEEEEEC
MNRGGYVKDFHVKGVSLPNGVTLKGGGYGSALLAGSPINASVPLGVVTPAAGNPSAAQGG
CCCCCEEEEEEEECCCCCCCEEEECCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCC
IVTFDCDYQPANDAVRTRAPVVQNVTIADVTARNVTLNGVTASCFQAIVAQGPVAFDYNG
EEEEECCCCCCCCHHHHCCCHHCCEEEEEEEECEEEECCCHHHHHHHHHHCCCEEEECCC
ASPAPTVQPIAGVTISHCDFGTPVASGTPTVTSPGPIYAFNVSGMTLRNVVIAGQTVNTT
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEEECCEEEEE
ITDSR
ECCCC
>Mature Secondary Structure 
AAVRRRPSSSVRSRSPKRLSRRAFLGWTGALAGGTVLGGPLAATRAWAGKLPASSAVDL
CCCCCCCCHHHHCCCHHHHHHHEEEEECHHHCCCCEECCCHHHHHHHHCCCCCCCCEEE
IWGEHGAAARIAASLAHVSRLAFRARDFDVTHYGARPCATVPQTSPYTNAAKSPVSPGAD
EECCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
RTTAPGAFDSRPAFLAAIDACVREGGGRVVVPPGNWYCAGPIVLQSNVNFHLSANCTIYF
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCEEECCCEEEECCCCEEEEEEEEEEE
SPNPADYAKDGPVDCGANGRLYYSRWQANDCLNYGAPVYARNAVNIALTGEGPTSVLNGQ
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCEEECCEEEEEEECCCCCHHCCCC
AMTPFAGSGNDSVCWWTYKGSSGAYGAASSVPSQAYPNPNNLDLRIVAPGISDALYAMLT
EECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
SPVTPWQQDQNYLPALSEAGVPVDKRIFGLGHYLRPCMVEFIGCTNVLMENYQTQNTPFW
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QHHPTACRNVVIRGVTTNSIGPNNDGFDPDACTDVLCEDCTFNTGDDCIAIKSGKNRDTE
CCCCHHHCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCC
YGPAKRHLVRNCTMNSGHGGITLGSEMGGGVEQIYATNLSMLNANWQTNPLNIAIRVKTN
CCHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHCCEEEEECCCCCCCEEEEEEEEEC
MNRGGYVKDFHVKGVSLPNGVTLKGGGYGSALLAGSPINASVPLGVVTPAAGNPSAAQGG
CCCCCEEEEEEEECCCCCCCEEEECCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCC
IVTFDCDYQPANDAVRTRAPVVQNVTIADVTARNVTLNGVTASCFQAIVAQGPVAFDYNG
EEEEECCCCCCCCHHHHCCCHHCCEEEEEEEECEEEECCCHHHHHHHHHHCCCEEEECCC
ASPAPTVQPIAGVTISHCDFGTPVASGTPTVTSPGPIYAFNVSGMTLRNVVIAGQTVNTT
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEEECCEEEEE
ITDSR
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]