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The map label for this gene is insK [H]

Identifier: 189353927

GI number: 189353927

Start: 2243391

End: 2244137

Strand: Reverse

Name: insK [H]

Synonym: BMULJ_05180

Alternate gene names: 189353927

Gene position: 2244137-2243391 (Counterclockwise)

Preceding gene: 189353928

Following gene: 189353921

Centisome position: 90.74

GC content: 56.76

Gene sequence:

>747_bases
TTGGATGCTGGCGATCGGTATGCGGAGCTCAAAGTGAAGATCCGGAGCGTGTACGACCACCACAAGGGCCGTTACGGCTA
TCGGCGAATCACGACAGTGATCCGGCAAGCGGGACACGCGATCAATCACAAGACCGTGCAGCGGTTGATGGGACAACTGC
AGTTGAAATCTCTGGTACGCCCGAAGAGATATCGTTCCTGGCGAGGCGAAGTCGGCCGCGTTGCCCCAAATCTACTGCGG
CGGCAGTTTGATGCTGAACGCCCGAATCAGAAATGGGTGACCGACGTGACCGAATTCAATGTAGGGGGCCAAAAGCTCTA
TCTGTCGCCGGTGATGGACTTGTACAACGGGGAGATCGTCGCTTACCAAATGGATGAGCGCCCGAGCTTCGAGTTGGTCA
GCGGCATGTTGAAGAAGGCGGTGGCCAAGCTCGATGATGAAGGACGGCCGTTACTGCATTCGGATCAAGGCTGGCAGTAT
CAGATGCCCGCCTACCGTCGGTTGTTGAAGCAGCATGCGCTCACCCAGAGCATGTCGCGCAGAGGCAACTGCCTTGATAA
CGCAGCCATGGAGAGCTTCTTCGGCACGCTGAAATCCGAGTGCTATCGGCTGGCGCGCTTTGCGAACGTGGAGCAGTTGC
GAGATGCGTTGAGCCGCTACATTCACTACTACAACCACGAACGCATCAAGCTCAAATTAAACGGGCTGAGTCCCGTGCAA
TACAGGACTCAGCCCTCGGCCGCTTAG

Upstream 100 bases:

>100_bases
AACGCAAATAATACGTGAGCTTAGGCAATGCCATCCGATCGCCGCGTTACTGAAGGCGGCAGGTCTGGCACGCAGCACGT
TCTATTACCAACTCAAGGTA

Downstream 100 bases:

>100_bases
ATTTTAACCGTCCAACTTCGTGGGGTCAGTTCACGCGGCGCCGTGCGCAGCCGCGCGCGCAAAAAAAACGGGCTCCTTGC
GGAGCCCGTCTGCGCGGATG

Product: ISBmu5a transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MDAGDRYAELKVKIRSVYDHHKGRYGYRRITTVIRQAGHAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLR
RQFDAERPNQKWVTDVTEFNVGGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSGMLKKAVAKLDDEGRPLLHSDQGWQY
QMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFFGTLKSECYRLARFANVEQLRDALSRYIHYYNHERIKLKLNGLSPVQ
YRTQPSAA

Sequences:

>Translated_248_residues
MDAGDRYAELKVKIRSVYDHHKGRYGYRRITTVIRQAGHAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLR
RQFDAERPNQKWVTDVTEFNVGGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSGMLKKAVAKLDDEGRPLLHSDQGWQY
QMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFFGTLKSECYRLARFANVEQLRDALSRYIHYYNHERIKLKLNGLSPVQ
YRTQPSAA
>Mature_248_residues
MDAGDRYAELKVKIRSVYDHHKGRYGYRRITTVIRQAGHAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLR
RQFDAERPNQKWVTDVTEFNVGGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSGMLKKAVAKLDDEGRPLLHSDQGWQY
QMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFFGTLKSECYRLARFANVEQLRDALSRYIHYYNHERIKLKLNGLSPVQ
YRTQPSAA

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=192, Percent_Identity=31.25, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1789981, Length=244, Percent_Identity=56.9672131147541, Blast_Score=317, Evalue=7e-88,
Organism=Escherichia coli, GI1788405, Length=233, Percent_Identity=33.9055793991416, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787263, Length=233, Percent_Identity=33.9055793991416, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786753, Length=233, Percent_Identity=33.9055793991416, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786570, Length=233, Percent_Identity=33.9055793991416, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786490, Length=233, Percent_Identity=33.9055793991416, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 28937; Mature: 28937

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCC
>Mature Secondary Structure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CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]