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The map label for this gene is argT [H]

Identifier: 189353926

GI number: 189353926

Start: 2242496

End: 2243278

Strand: Direct

Name: argT [H]

Synonym: BMULJ_05179

Alternate gene names: 189353926

Gene position: 2242496-2243278 (Clockwise)

Preceding gene: 189353925

Following gene: 189353929

Centisome position: 90.67

GC content: 64.37

Gene sequence:

>783_bases
GTGAAAAAGATTCTTGCGGCTGTGACCGTTGCCCTGCTCGCCGTATCGGCCGGCAGCGCCTACGCGAAAGACTGGACGAC
CGTGCGGTTCGGCGTCGACGCGAGCTACCCGCCTTTCGAGTCGAAAGACGCGAGCGGCAAGGTGGTCGGCTTCGACGTGG
ATCTCGGCAACGAAATCTGCCGCCGCCTGAACGCGAAGTGCGTGTGGATCGAGAACGACTTCGACGGGATGATCCCGGCG
CTGAAGGCCCGCAAGTTCGACGGCGTGCTGTCGTCGATGTCGATGACGCCGGCGCGTCAGGAACAGATCGCGTTCTCGTC
GAAGCTGTTCAACACGCCGACGCGCCTCGTCGCGAAGAAGGGCTCGGGCCTGATGCCGACGGCCGAATCGCTGAAGGGCA
AGTCGATCGGCGTCGAGCAGGGCACGATCCAGGAAACCTACGCGAAGACGTACTGGGCGCCGAAGGGCGTGAAGGTCGTC
CCGTACCAGAACCAGGACCAGGTGTACGCCGACCTGATCGCGGGCCGCCTCGACGGCGCGTTGCAGGACGCGGTGCAGGC
CGAGATCGGCTTCCTGAAGACGCCGCGCGGCGCGAACTTCGACTTCGCGGGCAAGGATCTCGACGATCCGAAGACGCTCG
GCGAAGGCGCGGGCATCGGCCTGCGCAAGGAAGACACCGACCTGAAGGCGAAGATCGACGCGGCCATCGCGGCGATGCGC
AAGGACGGCACGTACGACAAGATCGCGAAGAAGTACTTCGACTTCGACGTGTACGGCAAGTAA

Upstream 100 bases:

>100_bases
GGGCAGGCGAAACGGACAGTCCGACGCGAAGCCGCGCCGGGCCGAAGGCTTACGGCGCGGCACAACCGCGCACCCATCGA
ACAAAACGAGGAGTCACCGA

Downstream 100 bases:

>100_bases
GCGCGCGCGCCGCATCCGCGCAGACGGGCTCCGCAAGGAGCCCGTTTTTTTTGCGCGCGCGGCTGCGCACGGCGCCGCGT
GAACTGACCCCACGAAGTTG

Product: polar amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKKILAAVTVALLAVSAGSAYAKDWTTVRFGVDASYPPFESKDASGKVVGFDVDLGNEICRRLNAKCVWIENDFDGMIPA
LKARKFDGVLSSMSMTPARQEQIAFSSKLFNTPTRLVAKKGSGLMPTAESLKGKSIGVEQGTIQETYAKTYWAPKGVKVV
PYQNQDQVYADLIAGRLDGALQDAVQAEIGFLKTPRGANFDFAGKDLDDPKTLGEGAGIGLRKEDTDLKAKIDAAIAAMR
KDGTYDKIAKKYFDFDVYGK

Sequences:

>Translated_260_residues
MKKILAAVTVALLAVSAGSAYAKDWTTVRFGVDASYPPFESKDASGKVVGFDVDLGNEICRRLNAKCVWIENDFDGMIPA
LKARKFDGVLSSMSMTPARQEQIAFSSKLFNTPTRLVAKKGSGLMPTAESLKGKSIGVEQGTIQETYAKTYWAPKGVKVV
PYQNQDQVYADLIAGRLDGALQDAVQAEIGFLKTPRGANFDFAGKDLDDPKTLGEGAGIGLRKEDTDLKAKIDAAIAAMR
KDGTYDKIAKKYFDFDVYGK
>Mature_260_residues
MKKILAAVTVALLAVSAGSAYAKDWTTVRFGVDASYPPFESKDASGKVVGFDVDLGNEICRRLNAKCVWIENDFDGMIPA
LKARKFDGVLSSMSMTPARQEQIAFSSKLFNTPTRLVAKKGSGLMPTAESLKGKSIGVEQGTIQETYAKTYWAPKGVKVV
PYQNQDQVYADLIAGRLDGALQDAVQAEIGFLKTPRGANFDFAGKDLDDPKTLGEGAGIGLRKEDTDLKAKIDAAIAAMR
KDGTYDKIAKKYFDFDVYGK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=55.2123552123552, Blast_Score=301, Evalue=2e-83,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=53.6480686695279, Blast_Score=284, Evalue=4e-78,
Organism=Escherichia coli, GI1787085, Length=254, Percent_Identity=37.7952755905512, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=34.3873517786561, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788228, Length=268, Percent_Identity=29.4776119402985, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=31.2252964426877, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786876, Length=234, Percent_Identity=28.2051282051282, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28171; Mature: 28171

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILAAVTVALLAVSAGSAYAKDWTTVRFGVDASYPPFESKDASGKVVGFDVDLGNEIC
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCHHHHH
RRLNAKCVWIENDFDGMIPALKARKFDGVLSSMSMTPARQEQIAFSSKLFNTPTRLVAKK
HHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHC
GSGLMPTAESLKGKSIGVEQGTIQETYAKTYWAPKGVKVVPYQNQDQVYADLIAGRLDGA
CCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHH
LQDAVQAEIGFLKTPRGANFDFAGKDLDDPKTLGEGAGIGLRKEDTDLKAKIDAAIAAMR
HHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
KDGTYDKIAKKYFDFDVYGK
CCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKILAAVTVALLAVSAGSAYAKDWTTVRFGVDASYPPFESKDASGKVVGFDVDLGNEIC
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCHHHHH
RRLNAKCVWIENDFDGMIPALKARKFDGVLSSMSMTPARQEQIAFSSKLFNTPTRLVAKK
HHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHC
GSGLMPTAESLKGKSIGVEQGTIQETYAKTYWAPKGVKVVPYQNQDQVYADLIAGRLDGA
CCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHH
LQDAVQAEIGFLKTPRGANFDFAGKDLDDPKTLGEGAGIGLRKEDTDLKAKIDAAIAAMR
HHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
KDGTYDKIAKKYFDFDVYGK
CCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]