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The map label for this gene is hsrA [H]

Identifier: 189353839

GI number: 189353839

Start: 2144919

End: 2146343

Strand: Reverse

Name: hsrA [H]

Synonym: BMULJ_05086

Alternate gene names: 189353839

Gene position: 2146343-2144919 (Counterclockwise)

Preceding gene: 189353842

Following gene: 189353835

Centisome position: 86.79

GC content: 66.11

Gene sequence:

>1425_bases
ATGACTGCTCGTTCCGCCTCAGGTGATCGGACGACCGACTTCCTGCCGTATCTCGTCGCCGCGACGTTCTTCATGGAGTA
TCTCGACACCACCGTGATCGCGACCGCGCTGCCGCAAATGGCGCACACGTTCGGCGTCGGGCCGAATGCGCTCAGTCTCG
GCATGACGGCCTACATGCTCGCGCTTGCGGTCTTCATCCCGATCAGCGGCTGGATCGCCGACCGCTATGGCTCGAGAACG
GTGTTCGGCAGCGCCATCGTGGTCTTTACCGGCGCCTCCATACTGTGCGGTCTGTCGAACGGCGTCGGGACATTCACGGC
CGCGCGGCTGTTGCAGGGCGTCGGCGGAGCGATGATGGTGCCGGTCGGCCGGATGATCGTCGTGCGCAGCACCGAGAAAT
CGCGGCTCATGCGCGCGATCGCGACGATCACCTGGCCCGGCATCGTCGCGCCAGTCGTCGGACCACCGATCGGCGGCTTT
ATCACGACTTATGCGTCGTGGCGCTGGATCTTCCTGCTGAACGTGCCGTTCGGTATCGCCGCACTCGTCAGCACCTGGCT
CATCGTCCGGAACACGCGCGCGGAACAGCAGCGGCCGCTCGACTGGATCGGGTTCGTCCTGGCGGGCGGCGCCTTGACCT
GTCTGCTGCTCGGTACCGAAACGGCCGGACAGCAAAATGCGCGGTTCGCGCAGGCCGGCATGCTCGTCGGCGCCAGCGTG
CTGTTCGGCATTGCCGCGTGGGTTCATGCGCGGCGATGCGCACACCCGCTGCTGGACTTCACGACGCTGAAAGTGCCGAC
GTTCTCGGTCACCGTGATCACCGGATCGATCACGCGGATCGCGATCAACGCGGTGCCGTACCTGCTCCCGCTGCTGTTCC
AGATCGGCTTCGGACTATCGCCGTTTCAGTCGGGCCTGCTGCTGCTCGCGAGCGCACTCGGCAACCTCGGCATGAAGGCC
GGCACGTCGTGGATCCTCGATCGGTTCGGATTCCGCCGTGTCGCGCTCGTCGACGTGACCATTGTCGGCATCTGCACGAT
CGCTTGCGGCTGGCTCACCGCGTCGACGCCGCTCGCGATCACGCTGCTCGTCGTGTTCGTGTACGGGCTGACGCGATCGA
TGCAGTTCACGACGCTGGCGACGCTCGCGTATGCCGACATCCCCGCTCAGCAGACGAGCGCGGCAAGCACACTATGGAGC
GCCGCGCAGCAGATGACGATCGGCATGGGCATCGCATTCGGCGCGCTGTCGCTGCGCGTTGCGGCTTCGCTGCGTGGCGA
TACGGGTGGCGTTCACTATCTGCTCGCGGATTTTCGATGGGCCTTTGTCGCGGCCGGCGTGCTCGCGTTGCTGACGTTGC
CCGGTTACGCGCGGCTCGCCCGCGATGCGGGCGATCGGCTGCGAGCAAGCGCGGCACGCGGGTAA

Upstream 100 bases:

>100_bases
AATCACCCCTTCAGGGTAATATCGCGATTCGTGCCGGCTCCAGCTGCAACCGTCAGTGACGGCGGCCGGCGTTTCTGCAT
CCCCGTCTATTACACCGTTC

Downstream 100 bases:

>100_bases
GCGAGCGGCGCGCGGATATGGTCTGCATCCGCGCGGCGAAGTGTATGCAGTCTGCGCTCGTCGGCGAATATCGCAGTCGC
AGTGGCGCGGCCGTTACTGC

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTARSASGDRTTDFLPYLVAATFFMEYLDTTVIATALPQMAHTFGVGPNALSLGMTAYMLALAVFIPISGWIADRYGSRT
VFGSAIVVFTGASILCGLSNGVGTFTAARLLQGVGGAMMVPVGRMIVVRSTEKSRLMRAIATITWPGIVAPVVGPPIGGF
ITTYASWRWIFLLNVPFGIAALVSTWLIVRNTRAEQQRPLDWIGFVLAGGALTCLLLGTETAGQQNARFAQAGMLVGASV
LFGIAAWVHARRCAHPLLDFTTLKVPTFSVTVITGSITRIAINAVPYLLPLLFQIGFGLSPFQSGLLLLASALGNLGMKA
GTSWILDRFGFRRVALVDVTIVGICTIACGWLTASTPLAITLLVVFVYGLTRSMQFTTLATLAYADIPAQQTSAASTLWS
AAQQMTIGMGIAFGALSLRVAASLRGDTGGVHYLLADFRWAFVAAGVLALLTLPGYARLARDAGDRLRASAARG

Sequences:

>Translated_474_residues
MTARSASGDRTTDFLPYLVAATFFMEYLDTTVIATALPQMAHTFGVGPNALSLGMTAYMLALAVFIPISGWIADRYGSRT
VFGSAIVVFTGASILCGLSNGVGTFTAARLLQGVGGAMMVPVGRMIVVRSTEKSRLMRAIATITWPGIVAPVVGPPIGGF
ITTYASWRWIFLLNVPFGIAALVSTWLIVRNTRAEQQRPLDWIGFVLAGGALTCLLLGTETAGQQNARFAQAGMLVGASV
LFGIAAWVHARRCAHPLLDFTTLKVPTFSVTVITGSITRIAINAVPYLLPLLFQIGFGLSPFQSGLLLLASALGNLGMKA
GTSWILDRFGFRRVALVDVTIVGICTIACGWLTASTPLAITLLVVFVYGLTRSMQFTTLATLAYADIPAQQTSAASTLWS
AAQQMTIGMGIAFGALSLRVAASLRGDTGGVHYLLADFRWAFVAAGVLALLTLPGYARLARDAGDRLRASAARG
>Mature_473_residues
TARSASGDRTTDFLPYLVAATFFMEYLDTTVIATALPQMAHTFGVGPNALSLGMTAYMLALAVFIPISGWIADRYGSRTV
FGSAIVVFTGASILCGLSNGVGTFTAARLLQGVGGAMMVPVGRMIVVRSTEKSRLMRAIATITWPGIVAPVVGPPIGGFI
TTYASWRWIFLLNVPFGIAALVSTWLIVRNTRAEQQRPLDWIGFVLAGGALTCLLLGTETAGQQNARFAQAGMLVGASVL
FGIAAWVHARRCAHPLLDFTTLKVPTFSVTVITGSITRIAINAVPYLLPLLFQIGFGLSPFQSGLLLLASALGNLGMKAG
TSWILDRFGFRRVALVDVTIVGICTIACGWLTASTPLAITLLVVFVYGLTRSMQFTTLATLAYADIPAQQTSAASTLWSA
AQQMTIGMGIAFGALSLRVAASLRGDTGGVHYLLADFRWAFVAAGVLALLTLPGYARLARDAGDRLRASAARG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=463, Percent_Identity=32.1814254859611, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1788392, Length=408, Percent_Identity=35.7843137254902, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87081983, Length=310, Percent_Identity=27.4193548387097, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789042, Length=387, Percent_Identity=26.0981912144703, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788710, Length=389, Percent_Identity=24.6786632390745, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790146, Length=169, Percent_Identity=25.4437869822485, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=149, Percent_Identity=30.2013422818792, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=30.6666666666667, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=185, Percent_Identity=29.7297297297297, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50100; Mature: 49968

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARSASGDRTTDFLPYLVAATFFMEYLDTTVIATALPQMAHTFGVGPNALSLGMTAYML
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ALAVFIPISGWIADRYGSRTVFGSAIVVFTGASILCGLSNGVGTFTAARLLQGVGGAMMV
HHHHHHHHHHHHHHCCCCCCHHCCEEEEEECCHHEEECCCCCHHHHHHHHHHHCCCEEEE
PVGRMIVVRSTEKSRLMRAIATITWPGIVAPVVGPPIGGFITTYASWRWIFLLNVPFGIA
CCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHCCEEEEEEEECCHHHH
ALVSTWLIVRNTRAEQQRPLDWIGFVLAGGALTCLLLGTETAGQQNARFAQAGMLVGASV
HHHHHHHHHCCCCHHHHCCHHHHHHHHHCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHH
LFGIAAWVHARRCAHPLLDFTTLKVPTFSVTVITGSITRIAINAVPYLLPLLFQIGFGLS
HHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCC
PFQSGLLLLASALGNLGMKAGTSWILDRFGFRRVALVDVTIVGICTIACGWLTASTPLAI
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHH
TLLVVFVYGLTRSMQFTTLATLAYADIPAQQTSAASTLWSAAQQMTIGMGIAFGALSLRV
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASLRGDTGGVHYLLADFRWAFVAAGVLALLTLPGYARLARDAGDRLRASAARG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TARSASGDRTTDFLPYLVAATFFMEYLDTTVIATALPQMAHTFGVGPNALSLGMTAYML
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ALAVFIPISGWIADRYGSRTVFGSAIVVFTGASILCGLSNGVGTFTAARLLQGVGGAMMV
HHHHHHHHHHHHHHCCCCCCHHCCEEEEEECCHHEEECCCCCHHHHHHHHHHHCCCEEEE
PVGRMIVVRSTEKSRLMRAIATITWPGIVAPVVGPPIGGFITTYASWRWIFLLNVPFGIA
CCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHCCEEEEEEEECCHHHH
ALVSTWLIVRNTRAEQQRPLDWIGFVLAGGALTCLLLGTETAGQQNARFAQAGMLVGASV
HHHHHHHHHCCCCHHHHCCHHHHHHHHHCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHH
LFGIAAWVHARRCAHPLLDFTTLKVPTFSVTVITGSITRIAINAVPYLLPLLFQIGFGLS
HHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCC
PFQSGLLLLASALGNLGMKAGTSWILDRFGFRRVALVDVTIVGICTIACGWLTASTPLAI
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHH
TLLVVFVYGLTRSMQFTTLATLAYADIPAQQTSAASTLWSAAQQMTIGMGIAFGALSLRV
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASLRGDTGGVHYLLADFRWAFVAAGVLALLTLPGYARLARDAGDRLRASAARG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]