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The map label for this gene is metE [H]

Identifier: 189353835

GI number: 189353835

Start: 2138183

End: 2140477

Strand: Reverse

Name: metE [H]

Synonym: BMULJ_05082

Alternate gene names: 189353835

Gene position: 2140477-2138183 (Counterclockwise)

Preceding gene: 189353839

Following gene: 189353832

Centisome position: 86.55

GC content: 66.36

Gene sequence:

>2295_bases
ATGGTCACGACACACAACCTCGGTTTTCCACGCATCGGCGCGAAGCGCGAACTCAAGTTCGGTCTCGAACGCTACTGGAA
CGGAGAATCGTCGCGCGACGAACTGACGGCGCTCGGCGCCGCGCTGCGTCGGCAGCACTGGAACGATCAGCGCGGGCTCG
ACCTCGTGCCGATCGGCGATTTCGCGTTCTACGATCAGGTGCTCGACATGAGCTTCACGCTCGGCAATCTGCCGAAGCGC
GTGCGGGACTTTCACGGCGATCCGCTCGACAATTACTTCCGCGTCGCGCGCGGCCGTTCCGCCCAGTCCGCGGACGCGCA
CGCCGCGTGCTGCGGCGGCGTCGCGGCCGGCGAGATGACGAAGTGGTTCGACACGAACTATCACTACATCGTCCCGGAGT
TCCACGGCGATACGAACTTCTCCCTCGATCCGTCGCGGCTGCTTCAGCATCTCGCGGAAGCGCGCGCGGCGGGCGTGACC
GCGAAGCCCGTGATTCTCGGCCCCGTCACCTATCTGTGGCTCGGCAAGTCGAAGGACGATTCCGACCGGCTCGCGCTGCT
GCCGAAACTGCTGCCCGTGTACGGCGCGCTGCTCGATACGCTGACCGCGCAAGGCGTCGAGTGGGTGCAGGTCGACGAGC
CGATTCTCGTCACCGAACTCGATGCCGAATGGCGGCACGCGCTGCGCACCGCCTATGCGGCGCTCGAGACGCGCCGCATC
AAGCTGCTGCTCGCGACCTACTTCGGCGAGTTGCAGGACAACCTGACGCTTGCGGCGTCGCTACCGGTCGACGGCCTGCA
TGTCGATGCGATCAACGCGCGCGACGAGATCGCCGCAGTCGTGCGCGAACTGCCGGCGGACCGCGTGCTGTCGGTCGGTG
CGATCAACGGCCGCAATATCTGGAAGACGGATCTGAACGCGACGCTCGACTGGCTCGAACCGCTCGCGAAGCAGCTCGGC
GACCGGCTGTGGCTCGCGCCGTCGTGCTCGCTGCTGCACGTGCCGGTCGACCTCGCGAGCGAGGACCGCCTCGATGCCGA
CATCCGTTCGTGGCTCGCCTTCGCTTTGCAGAAACTCGACGAGCTGAAAGTGCTCGCGACCGCGCTGAACGAAGGACGCG
ACAAGGTTGCCGACGCGCTTGCCGCGAATGCCGCCGCGATCGACTCGCGTCGCCGCTCGCCGCGCGTGCACAACCCGGCG
GTGAAAGACGCGATCGCGCGGATCGACGCACGTCTCGGCGATCGCGCGAGCCCGTATCCGCAGCGCGCGGCGAAGCAAGC
CGCGCGACTGAATCTGCCGGCATTCCCGACGACGACGATCGGCTCGTTCCCGCAGACCGCGGAGATCCGGCACGCGCGCA
GCCGCTTCAAGGCGGGCACGCTCGACGAAGCCGGCTATCGCGCGGCGATGCGCGCCGAGATCGAGCGCAGCGTGCGCGAG
CAGGAGTCGCTCGAGCTCGACGTGCTCGTGCACGGCGAGGCCGAGCGCAACGACATGGTCGAATATTTCGGCGAGCAGCT
CGACGGATTCGCGTTCAGCCGGTTCGGCTGGGTGCAGTCGTACGGTTCGCGCTGCGTGAAGCCGCCGATCCTGTTCGGCG
ACATCAGCCGTCCGAATGCGATGACGGTCGAATGGATCACGTATGCGCAGTCGCTGACGCACAAGCCGATGAAGGGCATG
CTGACGGGCCCCGTGACGATCCTGAACTGGTCGTTCGTGCGCGACGACCAGCCGCGCTCGGTGTCCTGCCGTCAGCTCGC
GCTCGCGATCCGCGACGAAGTGCTCGATCTCGAGAAGGCCGGCGTACGCGTGATCCAGATCGACGAGGCGGCGTTGCGCG
AAGGGCTGCCGCTGCGCCGTTCGCAATGGAGTGCGTATCTGCAATGGGCGGTCGAGTCGTTCCGGATCGCCGCGAACGGC
GTGCGCGACGACACGCAGATCCACACGCACATGTGCTATTCGGAGTTCAACGACATCATCGCGTCGATCGCCGACATGGA
TGCCGACGTGATCACGATCGAAACGTCGCGCTCCGACATGGAACTGCTCGACGCGTTCGACAGCTTCAGGTATCCGAACG
AAATCGGGCCCGGCGTGTACGACATTCATTCGCCGAACATTCCGACGCAGGACCACATCGTCGCGCTGATGAAGAAGGCG
GCCGAGCGGATCCCGGCCGATCGCCTGTGGGTGAACCCGGACTGCGGGTTGAAGACGCGCCGGTGGGCGGAGGTGATTCC
GGCGCTGGCCAACATGGTGGCGGCCGCGAAGACGCTGCGCGCGCAGCAGCAGTAA

Upstream 100 bases:

>100_bases
GAACAGATTTGATGGATGAATGAAATAATGCCATTTTATTTCATGTGTTGAAATCCCTATCATGGCTGCCGATCCATCAT
TCCTTCAGAACGGCAGCTCC

Downstream 100 bases:

>100_bases
CGTTGCCGCGCGCGGCGCGCCGGGCAGGCGCTGAGGTCTGCCCGGCGAAGTGCGACGTTTTACGCCGTACGCGCGGCGGT
GTGCGCGCCGGCGCGCGGCA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MVTTHNLGFPRIGAKRELKFGLERYWNGESSRDELTALGAALRRQHWNDQRGLDLVPIGDFAFYDQVLDMSFTLGNLPKR
VRDFHGDPLDNYFRVARGRSAQSADAHAACCGGVAAGEMTKWFDTNYHYIVPEFHGDTNFSLDPSRLLQHLAEARAAGVT
AKPVILGPVTYLWLGKSKDDSDRLALLPKLLPVYGALLDTLTAQGVEWVQVDEPILVTELDAEWRHALRTAYAALETRRI
KLLLATYFGELQDNLTLAASLPVDGLHVDAINARDEIAAVVRELPADRVLSVGAINGRNIWKTDLNATLDWLEPLAKQLG
DRLWLAPSCSLLHVPVDLASEDRLDADIRSWLAFALQKLDELKVLATALNEGRDKVADALAANAAAIDSRRRSPRVHNPA
VKDAIARIDARLGDRASPYPQRAAKQAARLNLPAFPTTTIGSFPQTAEIRHARSRFKAGTLDEAGYRAAMRAEIERSVRE
QESLELDVLVHGEAERNDMVEYFGEQLDGFAFSRFGWVQSYGSRCVKPPILFGDISRPNAMTVEWITYAQSLTHKPMKGM
LTGPVTILNWSFVRDDQPRSVSCRQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLRRSQWSAYLQWAVESFRIAANG
VRDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAFDSFRYPNEIGPGVYDIHSPNIPTQDHIVALMKKA
AERIPADRLWVNPDCGLKTRRWAEVIPALANMVAAAKTLRAQQQ

Sequences:

>Translated_764_residues
MVTTHNLGFPRIGAKRELKFGLERYWNGESSRDELTALGAALRRQHWNDQRGLDLVPIGDFAFYDQVLDMSFTLGNLPKR
VRDFHGDPLDNYFRVARGRSAQSADAHAACCGGVAAGEMTKWFDTNYHYIVPEFHGDTNFSLDPSRLLQHLAEARAAGVT
AKPVILGPVTYLWLGKSKDDSDRLALLPKLLPVYGALLDTLTAQGVEWVQVDEPILVTELDAEWRHALRTAYAALETRRI
KLLLATYFGELQDNLTLAASLPVDGLHVDAINARDEIAAVVRELPADRVLSVGAINGRNIWKTDLNATLDWLEPLAKQLG
DRLWLAPSCSLLHVPVDLASEDRLDADIRSWLAFALQKLDELKVLATALNEGRDKVADALAANAAAIDSRRRSPRVHNPA
VKDAIARIDARLGDRASPYPQRAAKQAARLNLPAFPTTTIGSFPQTAEIRHARSRFKAGTLDEAGYRAAMRAEIERSVRE
QESLELDVLVHGEAERNDMVEYFGEQLDGFAFSRFGWVQSYGSRCVKPPILFGDISRPNAMTVEWITYAQSLTHKPMKGM
LTGPVTILNWSFVRDDQPRSVSCRQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLRRSQWSAYLQWAVESFRIAANG
VRDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAFDSFRYPNEIGPGVYDIHSPNIPTQDHIVALMKKA
AERIPADRLWVNPDCGLKTRRWAEVIPALANMVAAAKTLRAQQQ
>Mature_764_residues
MVTTHNLGFPRIGAKRELKFGLERYWNGESSRDELTALGAALRRQHWNDQRGLDLVPIGDFAFYDQVLDMSFTLGNLPKR
VRDFHGDPLDNYFRVARGRSAQSADAHAACCGGVAAGEMTKWFDTNYHYIVPEFHGDTNFSLDPSRLLQHLAEARAAGVT
AKPVILGPVTYLWLGKSKDDSDRLALLPKLLPVYGALLDTLTAQGVEWVQVDEPILVTELDAEWRHALRTAYAALETRRI
KLLLATYFGELQDNLTLAASLPVDGLHVDAINARDEIAAVVRELPADRVLSVGAINGRNIWKTDLNATLDWLEPLAKQLG
DRLWLAPSCSLLHVPVDLASEDRLDADIRSWLAFALQKLDELKVLATALNEGRDKVADALAANAAAIDSRRRSPRVHNPA
VKDAIARIDARLGDRASPYPQRAAKQAARLNLPAFPTTTIGSFPQTAEIRHARSRFKAGTLDEAGYRAAMRAEIERSVRE
QESLELDVLVHGEAERNDMVEYFGEQLDGFAFSRFGWVQSYGSRCVKPPILFGDISRPNAMTVEWITYAQSLTHKPMKGM
LTGPVTILNWSFVRDDQPRSVSCRQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLRRSQWSAYLQWAVESFRIAANG
VRDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAFDSFRYPNEIGPGVYDIHSPNIPTQDHIVALMKKA
AERIPADRLWVNPDCGLKTRRWAEVIPALANMVAAAKTLRAQQQ

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=764, Percent_Identity=58.9005235602094, Blast_Score=880, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=774, Percent_Identity=48.1912144702842, Blast_Score=715, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85236; Mature: 85236

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTTHNLGFPRIGAKRELKFGLERYWNGESSRDELTALGAALRRQHWNDQRGLDLVPIGD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCC
FAFYDQVLDMSFTLGNLPKRVRDFHGDPLDNYFRVARGRSAQSADAHAACCGGVAAGEMT
HHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCHHCCHH
KWFDTNYHYIVPEFHGDTNFSLDPSRLLQHLAEARAAGVTAKPVILGPVTYLWLGKSKDD
HHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCEEEEEECCCCCC
SDRLALLPKLLPVYGALLDTLTAQGVEWVQVDEPILVTELDAEWRHALRTAYAALETRRI
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHH
KLLLATYFGELQDNLTLAASLPVDGLHVDAINARDEIAAVVRELPADRVLSVGAINGRNI
HHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCHHHHHHHHHHCCHHHEEEEECCCCCCC
WKTDLNATLDWLEPLAKQLGDRLWLAPSCSLLHVPVDLASEDRLDADIRSWLAFALQKLD
EECCHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
ELKVLATALNEGRDKVADALAANAAAIDSRRRSPRVHNPAVKDAIARIDARLGDRASPYP
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCH
QRAAKQAARLNLPAFPTTTIGSFPQTAEIRHARSRFKAGTLDEAGYRAAMRAEIERSVRE
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
QESLELDVLVHGEAERNDMVEYFGEQLDGFAFSRFGWVQSYGSRCVKPPILFGDISRPNA
HHCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCC
MTVEWITYAQSLTHKPMKGMLTGPVTILNWSFVRDDQPRSVSCRQLALAIRDEVLDLEKA
EEEHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GVRVIQIDEAALREGLPLRRSQWSAYLQWAVESFRIAANGVRDDTQIHTHMCYSEFNDII
CEEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ASIADMDADVITIETSRSDMELLDAFDSFRYPNEIGPGVYDIHSPNIPTQDHIVALMKKA
HHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
AERIPADRLWVNPDCGLKTRRWAEVIPALANMVAAAKTLRAQQQ
HHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTTHNLGFPRIGAKRELKFGLERYWNGESSRDELTALGAALRRQHWNDQRGLDLVPIGD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCC
FAFYDQVLDMSFTLGNLPKRVRDFHGDPLDNYFRVARGRSAQSADAHAACCGGVAAGEMT
HHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCHHCCHH
KWFDTNYHYIVPEFHGDTNFSLDPSRLLQHLAEARAAGVTAKPVILGPVTYLWLGKSKDD
HHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCEEEEEECCCCCC
SDRLALLPKLLPVYGALLDTLTAQGVEWVQVDEPILVTELDAEWRHALRTAYAALETRRI
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHH
KLLLATYFGELQDNLTLAASLPVDGLHVDAINARDEIAAVVRELPADRVLSVGAINGRNI
HHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCHHHHHHHHHHCCHHHEEEEECCCCCCC
WKTDLNATLDWLEPLAKQLGDRLWLAPSCSLLHVPVDLASEDRLDADIRSWLAFALQKLD
EECCHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
ELKVLATALNEGRDKVADALAANAAAIDSRRRSPRVHNPAVKDAIARIDARLGDRASPYP
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCH
QRAAKQAARLNLPAFPTTTIGSFPQTAEIRHARSRFKAGTLDEAGYRAAMRAEIERSVRE
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
QESLELDVLVHGEAERNDMVEYFGEQLDGFAFSRFGWVQSYGSRCVKPPILFGDISRPNA
HHCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCC
MTVEWITYAQSLTHKPMKGMLTGPVTILNWSFVRDDQPRSVSCRQLALAIRDEVLDLEKA
EEEHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GVRVIQIDEAALREGLPLRRSQWSAYLQWAVESFRIAANGVRDDTQIHTHMCYSEFNDII
CEEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ASIADMDADVITIETSRSDMELLDAFDSFRYPNEIGPGVYDIHSPNIPTQDHIVALMKKA
HHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
AERIPADRLWVNPDCGLKTRRWAEVIPALANMVAAAKTLRAQQQ
HHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA