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The map label for this gene is puuB [H]

Identifier: 189353820

GI number: 189353820

Start: 2121015

End: 2122283

Strand: Direct

Name: puuB [H]

Synonym: BMULJ_05067

Alternate gene names: 189353820

Gene position: 2121015-2122283 (Clockwise)

Preceding gene: 189353819

Following gene: 189353821

Centisome position: 85.76

GC content: 71.39

Gene sequence:

>1269_bases
ATGCAGAACTTCTACGAAGCCACCGTTACCCGCCCGCCCTATCCGCAACTGAGCGGCACGCTCGACACGCAGGTCTGCAT
CGTCGGCGGCGGCCTTGCCGGCCTGTGCACGGCGCTCGGCCTCGTCGAGCGCGGTGTGCGCGACGTGATCGTGCTCGACG
GCGAGCGCGTCGGCTTCGGCGCCTCGGGCCGCAACGGCGGCTTCGTGTTCGGCGGCTACAGCCTCGACAACGCGGATCTG
CTGCGCACGCTCGGCCGCGACGAAGCGCGCCGCCTGTACCGGCTGACGGTCGACGCGGTCGACCTGATCCGCGCGCGCAT
CGCGCGCTACGGGATCGACTGCGACATCGTCGATCGCGGCGTGATGCTCGCGAACTGGTTCGACGATCCGTCGCGGCTCG
ACAGTGTGCGTTCGCTGATGAAGCGCGAATTCGACGTTGACTGGGAACCGGTGACGCGCGACGCGTTGCGCGCGCAACTG
CATACCGCGCGCTACCACGGCGGTCTGTTCGAGCCGAACGCCTTCCACTTCCATCCGCTGAAGTACGTGCTCGGCGTCGC
GGCGGCCGCCGCACGCGGCGGCGCGCGCATCTACGAACGCTCGGCCGCGCTCGGCATCGCGCGCGAAGGCGCGGGCTACG
TCGTGCGCACGGCGCACGGCAGCGTGCGCGCGAAGGACGTCGTGTTCGCGGGCGGCGGCTACGCGCGCGGCGTGTCGCCG
CGCATCGAGCGCGCGGTGCTGCCGATCGCGACCTACGTGATCGCCACCGAGCCGCTCGGCGAACGGCTGAAGGACGCGAT
CGACGCGCCGTATGCGATCTACGACACGCGTTTCGCATTCGACTACTACCGGCCGCTGAAGGACACGCGGATCCTGTGGG
GCGGCCGCATCTCGGTGCTCGACCGCGGCCCCGACGCGATCGCGCGGCTGCTGCGCCGCGACCTGCTGCGCGTGTATCCG
CAGCTCGCGGACGCCAAGGTCGATTACGCCTGGGGCGGACTGATGAGCTATGCGCGGCACAAGATGCCGCAGATCGGCCG
CGACGCCGACGGCGTCTGGCATGCGATCGCGTTCGGCGGCCACGGGATGGCGCCGACCACGGTGGCCGGCGAAACGCTCG
CCGCTGCGCTGGCCGAAGACCGGCCGGTGCCGGACGGCTTTCGCGCCTTCGGCCTCACGCGCACGTTCGGGCTCGCAGGG
CTCGCGGCCGCGCAGCTCACCTACACCGCGTACCAGGCGCGCGACGCGCTCGCCGCGTACCGCCGATGA

Upstream 100 bases:

>100_bases
CGACCGGCGCCACCCGAACACCATTCGACGCGGCGCGCGCGTTCAGGCAAGATCGACGCGCGCCGCCCGTTTTTTTCGTC
GTCACGACTTGCCGAATCGC

Downstream 100 bases:

>100_bases
GCGTCGCGGGCCGCGCTGACGCGCTCGGAACGGCGCCGGAAAGCGAGCCGGCGCTCGACGAATCGTCGATTTTCCGCATG
CTAGAATGCCCGTTCCACGC

Product: glycine/D-amino acid oxidase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MQNFYEATVTRPPYPQLSGTLDTQVCIVGGGLAGLCTALGLVERGVRDVIVLDGERVGFGASGRNGGFVFGGYSLDNADL
LRTLGRDEARRLYRLTVDAVDLIRARIARYGIDCDIVDRGVMLANWFDDPSRLDSVRSLMKREFDVDWEPVTRDALRAQL
HTARYHGGLFEPNAFHFHPLKYVLGVAAAAARGGARIYERSAALGIAREGAGYVVRTAHGSVRAKDVVFAGGGYARGVSP
RIERAVLPIATYVIATEPLGERLKDAIDAPYAIYDTRFAFDYYRPLKDTRILWGGRISVLDRGPDAIARLLRRDLLRVYP
QLADAKVDYAWGGLMSYARHKMPQIGRDADGVWHAIAFGGHGMAPTTVAGETLAAALAEDRPVPDGFRAFGLTRTFGLAG
LAAAQLTYTAYQARDALAAYRR

Sequences:

>Translated_422_residues
MQNFYEATVTRPPYPQLSGTLDTQVCIVGGGLAGLCTALGLVERGVRDVIVLDGERVGFGASGRNGGFVFGGYSLDNADL
LRTLGRDEARRLYRLTVDAVDLIRARIARYGIDCDIVDRGVMLANWFDDPSRLDSVRSLMKREFDVDWEPVTRDALRAQL
HTARYHGGLFEPNAFHFHPLKYVLGVAAAAARGGARIYERSAALGIAREGAGYVVRTAHGSVRAKDVVFAGGGYARGVSP
RIERAVLPIATYVIATEPLGERLKDAIDAPYAIYDTRFAFDYYRPLKDTRILWGGRISVLDRGPDAIARLLRRDLLRVYP
QLADAKVDYAWGGLMSYARHKMPQIGRDADGVWHAIAFGGHGMAPTTVAGETLAAALAEDRPVPDGFRAFGLTRTFGLAG
LAAAQLTYTAYQARDALAAYRR
>Mature_422_residues
MQNFYEATVTRPPYPQLSGTLDTQVCIVGGGLAGLCTALGLVERGVRDVIVLDGERVGFGASGRNGGFVFGGYSLDNADL
LRTLGRDEARRLYRLTVDAVDLIRARIARYGIDCDIVDRGVMLANWFDDPSRLDSVRSLMKREFDVDWEPVTRDALRAQL
HTARYHGGLFEPNAFHFHPLKYVLGVAAAAARGGARIYERSAALGIAREGAGYVVRTAHGSVRAKDVVFAGGGYARGVSP
RIERAVLPIATYVIATEPLGERLKDAIDAPYAIYDTRFAFDYYRPLKDTRILWGGRISVLDRGPDAIARLLRRDLLRVYP
QLADAKVDYAWGGLMSYARHKMPQIGRDADGVWHAIAFGGHGMAPTTVAGETLAAALAEDRPVPDGFRAFGLTRTFGLAG
LAAAQLTYTAYQARDALAAYRR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=389, Percent_Identity=36.5038560411311, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46101; Mature: 46101

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFYEATVTRPPYPQLSGTLDTQVCIVGGGLAGLCTALGLVERGVRDVIVLDGERVGFG
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCEECCC
ASGRNGGFVFGGYSLDNADLLRTLGRDEARRLYRLTVDAVDLIRARIARYGIDCDIVDRG
CCCCCCCEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
VMLANWFDDPSRLDSVRSLMKREFDVDWEPVTRDALRAQLHTARYHGGLFEPNAFHFHPL
EEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECHH
KYVLGVAAAAARGGARIYERSAALGIAREGAGYVVRTAHGSVRAKDVVFAGGGYARGVSP
HHHHHHHHHHHCCCHHHHHHHHHHCEEECCCCEEEEECCCCCEECEEEEECCCCCCCCCH
RIERAVLPIATYVIATEPLGERLKDAIDAPYAIYDTRFAFDYYRPLKDTRILWGGRISVL
HHHHHHHHHHHHHEECCHHHHHHHHHHCCCEEEEECEEHHHHHCCCCCCEEEECCEEEEE
DRGPDAIARLLRRDLLRVYPQLADAKVDYAWGGLMSYARHKMPQIGRDADGVWHAIAFGG
ECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
HGMAPTTVAGETLAAALAEDRPVPDGFRAFGLTRTFGLAGLAAAQLTYTAYQARDALAAY
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RR
CC
>Mature Secondary Structure
MQNFYEATVTRPPYPQLSGTLDTQVCIVGGGLAGLCTALGLVERGVRDVIVLDGERVGFG
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCEECCC
ASGRNGGFVFGGYSLDNADLLRTLGRDEARRLYRLTVDAVDLIRARIARYGIDCDIVDRG
CCCCCCCEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
VMLANWFDDPSRLDSVRSLMKREFDVDWEPVTRDALRAQLHTARYHGGLFEPNAFHFHPL
EEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECHH
KYVLGVAAAAARGGARIYERSAALGIAREGAGYVVRTAHGSVRAKDVVFAGGGYARGVSP
HHHHHHHHHHHCCCHHHHHHHHHHCEEECCCCEEEEECCCCCEECEEEEECCCCCCCCCH
RIERAVLPIATYVIATEPLGERLKDAIDAPYAIYDTRFAFDYYRPLKDTRILWGGRISVL
HHHHHHHHHHHHHEECCHHHHHHHHHHCCCEEEEECEEHHHHHCCCCCCEEEECCEEEEE
DRGPDAIARLLRRDLLRVYPQLADAKVDYAWGGLMSYARHKMPQIGRDADGVWHAIAFGG
ECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
HGMAPTTVAGETLAAALAEDRPVPDGFRAFGLTRTFGLAGLAAAQLTYTAYQARDALAAY
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]