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The map label for this gene is galE [C]

Identifier: 189353775

GI number: 189353775

Start: 2056715

End: 2057686

Strand: Direct

Name: galE [C]

Synonym: BMULJ_05022

Alternate gene names: 189353775

Gene position: 2056715-2057686 (Clockwise)

Preceding gene: 189353774

Following gene: 189353778

Centisome position: 83.16

GC content: 69.34

Gene sequence:

>972_bases
ATGAAAGTACTGATCACCGGCGGCGCCGGCTTTCTCGGCCAGCGGCTCGCGCGCAAGCTGCTCGAGCGCGGCGAACTGAC
CGGCCCCGACGGCCGGCCGGAAAAGCTCGACGAGCTGGTGCTGCTCGACGTCGTCGCCGGCAGCGACTTCGGCGACGCGC
GCGTGAGCTCGATCGTCGGCGACATCGCCGATCGCGCCGTGCTCGAGCGCGCGATCGACGCACAAACGGGCGCGATCTTC
CATCTCGCGGCGATCGTCAGCGGGCAAGCCGAAGCCGACTTCGATCTCGGCATGCGCATCAACCTCGATGCGTCGCGCAC
GTTGCTCGAAGTGTGCCGCGCGCGCGGGCATCGGCCGCGTGTCGTGTTCACGAGCTCGGTCGCCGTATACGGCGGCACGC
TGCCGGACGTCGTGCAGGACGATACCGCGCTGAACCCGCAATCGTCGTACGGCGCGCAGAAGGCGATCGCCGAGCTGCTG
CTGTGCGACTACGCGCGACGCGGGTTCGTCGACGGCCGCGTGCTGCGTCTGCCGACCATCAGCGTGCGGCCCGGCCGGCC
GAACGCGGCGGCTTCGTCGTTCGCGAGCGGGATCATTCGCGAGCCGCTGAACGGCGAGGAAAGCGTGTGCCCGGTGCCGG
GCTCGACGCGGCTGTGGCTGCTGTCGCCGCGCGCGGCGATCGACGCGCTGGTCGCCGGTTGCGAGCTCGATGCCGCACAG
CTCGGCAACAAGCGCGTGATCAACCTGCCCGGCATTTCGGTGTCGGTCGACGAGATGATCGCGGCGCTGCGCGAAGTGGC
CGGCGACGAAGTCGTGAAGCGGATCCGTCATGTGCCGGACGAGCGCGTCGAGAAGATCGTCGGCAGCTGGCCCGGCCGCT
GGGACACGACGCGCGCGCATGCGCTCGGTCTGAAAGGCGATGCATCGTTCGCGGACGTGATTCGCAGCTATATCGACGAC
GAACGGCGCTGA

Upstream 100 bases:

>100_bases
TGCGAATATCGGCCGAAGGCCGGCACGTCGGCCGGCCTCGGCTGGCTCAAACCGTACCTGTAATTCGCGTATCTCACGTA
TTCCTGCGAGGGCAACCAAC

Downstream 100 bases:

>100_bases
GGGCGTGGCGCTTCGGCGCCGTGGTGCGCGGTGCGCGGTGCGAGGCACGAGGCGCATGCGTGGCCCACGCACATCGCACG
CGCGGCCGCGACATGCGGGC

Product: UDP-glucose 4-epimerase

Products: ADP-L-glycero-D-manno-heptose [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKVLITGGAGFLGQRLARKLLERGELTGPDGRPEKLDELVLLDVVAGSDFGDARVSSIVGDIADRAVLERAIDAQTGAIF
HLAAIVSGQAEADFDLGMRINLDASRTLLEVCRARGHRPRVVFTSSVAVYGGTLPDVVQDDTALNPQSSYGAQKAIAELL
LCDYARRGFVDGRVLRLPTISVRPGRPNAAASSFASGIIREPLNGEESVCPVPGSTRLWLLSPRAAIDALVAGCELDAAQ
LGNKRVINLPGISVSVDEMIAALREVAGDEVVKRIRHVPDERVEKIVGSWPGRWDTTRAHALGLKGDASFADVIRSYIDD
ERR

Sequences:

>Translated_323_residues
MKVLITGGAGFLGQRLARKLLERGELTGPDGRPEKLDELVLLDVVAGSDFGDARVSSIVGDIADRAVLERAIDAQTGAIF
HLAAIVSGQAEADFDLGMRINLDASRTLLEVCRARGHRPRVVFTSSVAVYGGTLPDVVQDDTALNPQSSYGAQKAIAELL
LCDYARRGFVDGRVLRLPTISVRPGRPNAAASSFASGIIREPLNGEESVCPVPGSTRLWLLSPRAAIDALVAGCELDAAQ
LGNKRVINLPGISVSVDEMIAALREVAGDEVVKRIRHVPDERVEKIVGSWPGRWDTTRAHALGLKGDASFADVIRSYIDD
ERR
>Mature_323_residues
MKVLITGGAGFLGQRLARKLLERGELTGPDGRPEKLDELVLLDVVAGSDFGDARVSSIVGDIADRAVLERAIDAQTGAIF
HLAAIVSGQAEADFDLGMRINLDASRTLLEVCRARGHRPRVVFTSSVAVYGGTLPDVVQDDTALNPQSSYGAQKAIAELL
LCDYARRGFVDGRVLRLPTISVRPGRPNAAASSFASGIIREPLNGEESVCPVPGSTRLWLLSPRAAIDALVAGCELDAAQ
LGNKRVINLPGISVSVDEMIAALREVAGDEVVKRIRHVPDERVEKIVGSWPGRWDTTRAHALGLKGDASFADVIRSYIDD
ERR

Specific function: Biosynthesis Of The Lipopolysaccharide Precursor ADP-L- Glycerol-D-Mannoheptose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI32566934, Length=306, Percent_Identity=25.4901960784314, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667531, Length=331, Percent_Identity=26.2839879154079, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.20 [C]

Molecular weight: Translated: 34621; Mature: 34621

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLITGGAGFLGQRLARKLLERGELTGPDGRPEKLDELVLLDVVAGSDFGDARVSSIVG
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEEHHHCCCCCCHHHHHHHHH
DIADRAVLERAIDAQTGAIFHLAAIVSGQAEADFDLGMRINLDASRTLLEVCRARGHRPR
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCE
VVFTSSVAVYGGTLPDVVQDDTALNPQSSYGAQKAIAELLLCDYARRGFVDGRVLRLPTI
EEEECCEEEECCCCCHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCEE
SVRPGRPNAAASSFASGIIREPLNGEESVCPVPGSTRLWLLSPRAAIDALVAGCELDAAQ
EECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHH
LGNKRVINLPGISVSVDEMIAALREVAGDEVVKRIRHVPDERVEKIVGSWPGRWDTTRAH
CCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHH
ALGLKGDASFADVIRSYIDDERR
EECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVLITGGAGFLGQRLARKLLERGELTGPDGRPEKLDELVLLDVVAGSDFGDARVSSIVG
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEEHHHCCCCCCHHHHHHHHH
DIADRAVLERAIDAQTGAIFHLAAIVSGQAEADFDLGMRINLDASRTLLEVCRARGHRPR
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCE
VVFTSSVAVYGGTLPDVVQDDTALNPQSSYGAQKAIAELLLCDYARRGFVDGRVLRLPTI
EEEECCEEEECCCCCHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCEE
SVRPGRPNAAASSFASGIIREPLNGEESVCPVPGSTRLWLLSPRAAIDALVAGCELDAAQ
EECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHH
LGNKRVINLPGISVSVDEMIAALREVAGDEVVKRIRHVPDERVEKIVGSWPGRWDTTRAH
CCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHH
ALGLKGDASFADVIRSYIDDERR
EECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.1 {ADP-D-glycero-D-manno-heptose}} [C]

Substrates: ADP-D-glycero-D-manno-heptose [C]

Specific reaction: ADP-D-glycero-D-manno-heptose --> ADP-L-glycero-D-manno-heptose [C]

General reaction: Epimerization [C]

Inhibitor: ADP; ADP glucose; ATP; NADH [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]