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The map label for this gene is fdhA2 [H]

Identifier: 189353733

GI number: 189353733

Start: 2002924

End: 2004120

Strand: Reverse

Name: fdhA2 [H]

Synonym: BMULJ_04978

Alternate gene names: 189353733

Gene position: 2004120-2002924 (Counterclockwise)

Preceding gene: 189353740

Following gene: 189353732

Centisome position: 81.03

GC content: 67.08

Gene sequence:

>1197_bases
ATGAGCAGCAATCGAGGTGTCGTCTATCTTGGGCCGGGCAAGGTCGAGGTGCAGAAGATCGATTATCCGAAGATGGTCGA
CCCGAGCGGCCGCGCAATCGGACACGGCGTGATCCTGAAGGTCGTCAGCACGAACATCTGCGGCTCGGACCAGCACATGG
TGCGCGGCCGCACGACGGCGCCGGTCGGTCTCGTGCTCGGTCACGAGATCACGGGCGAGGTCGTCGAGGTCGGTCGCGAC
GTCGAGACGCTGAAGATCGGCGATCTCGTGTCGGTGCCGTTCAACGTCGCGTGCGGCCGCTGCGCGATGTGCAAGGAAAC
GCATACGGGCGTGTGCCTGAACGTGAACCCGTCGCGCGCGGGCGGCGCGTACGGCTACGTCGACATGGGCGGCTGGATCG
GCGGCCAGGCCGAATACGTGCTCGTGCCGTATGCCGACTTCAACCTGCTGAAATTCCCGGACCGCGATCAGGCGATGGCG
AAGATCCGCGATCTGACCTGCCTGTCCGACATTCTGCCGACCGGTTATCACGGCGCGGTCAGCGCCGGCGTGAAGCCGGG
CTCGACCGTCTACATCGCGGGCGCGGGCCCGGTCGGGATGGCGGCGGCCGCCTCGGCGCGCCTGCTCGGCGCGGCCGTGA
CGATCGTCGGCGACATGAACGCGGAACGCCTCGCGCATGCGAAGGCGATGGGCTTCGAGACGGTCGACCTGTCGAAGGAC
GCGACGCTCGGCGAACAGATCGCGCAGATCCTCGGCAAGCCGGAGATCGACTGCGCGGTCGACTGCGTCGGCTTCGAGGC
GCACGGCCACGGCTCGTCGGGCCATGCGGAAGAAGCGCCCGCGACGGTGCTGAACTCGCTGATGGAAATCACGCGGCCGG
CCGGTGCGATCGGCATTCCGGGCCTGTACGTGACGGACGATCCGGGCGCGCAGGACAAGGCCGCGCAGCACGGCAGCCTG
AGCATCCGCTTCGGTCTCGGCTGGGCGAAGTCACATTCGTTCTTCACGGGCCAGACGCCGGTGCTGAAGTACAACCGCAA
CCTGATGCAGGCGATCCTGTACGACCGCCTGCCGATCGCGAAGATCGTCAACGTGACGGTGATCTCGCTCGACGACGCGC
CCGAAGGCTACAAGAAGTTCGACGGCGGCGCGCCGCGCAAATTCGTGATCGATCCGCACGGGCTGCTCGCGGCCTGA

Upstream 100 bases:

>100_bases
GCGCCCCGGCCTCCCGGACTACTCTCGGACAGCACGTCGCGCGTGTTCGGCAACCGCCGCAGCGCAGCGGCGCCATCCAA
CGAGACATGGAGACAATACG

Downstream 100 bases:

>100_bases
CGCGCAGCGCACGCACCCACGAAGCGGGCGGCACCGGAAACGGGCCGCCCGTTTGTTCTGTGGCGGCCGCGATGCTGTGC
GGCTGCGCGTTTTTCCGCGC

Product: glutathione-independent formaldehyde dehydrogenase

Products: NA

Alternate protein names: FALDH; FDH [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSSNRGVVYLGPGKVEVQKIDYPKMVDPSGRAIGHGVILKVVSTNICGSDQHMVRGRTTAPVGLVLGHEITGEVVEVGRD
VETLKIGDLVSVPFNVACGRCAMCKETHTGVCLNVNPSRAGGAYGYVDMGGWIGGQAEYVLVPYADFNLLKFPDRDQAMA
KIRDLTCLSDILPTGYHGAVSAGVKPGSTVYIAGAGPVGMAAAASARLLGAAVTIVGDMNAERLAHAKAMGFETVDLSKD
ATLGEQIAQILGKPEIDCAVDCVGFEAHGHGSSGHAEEAPATVLNSLMEITRPAGAIGIPGLYVTDDPGAQDKAAQHGSL
SIRFGLGWAKSHSFFTGQTPVLKYNRNLMQAILYDRLPIAKIVNVTVISLDDAPEGYKKFDGGAPRKFVIDPHGLLAA

Sequences:

>Translated_398_residues
MSSNRGVVYLGPGKVEVQKIDYPKMVDPSGRAIGHGVILKVVSTNICGSDQHMVRGRTTAPVGLVLGHEITGEVVEVGRD
VETLKIGDLVSVPFNVACGRCAMCKETHTGVCLNVNPSRAGGAYGYVDMGGWIGGQAEYVLVPYADFNLLKFPDRDQAMA
KIRDLTCLSDILPTGYHGAVSAGVKPGSTVYIAGAGPVGMAAAASARLLGAAVTIVGDMNAERLAHAKAMGFETVDLSKD
ATLGEQIAQILGKPEIDCAVDCVGFEAHGHGSSGHAEEAPATVLNSLMEITRPAGAIGIPGLYVTDDPGAQDKAAQHGSL
SIRFGLGWAKSHSFFTGQTPVLKYNRNLMQAILYDRLPIAKIVNVTVISLDDAPEGYKKFDGGAPRKFVIDPHGLLAA
>Mature_397_residues
SSNRGVVYLGPGKVEVQKIDYPKMVDPSGRAIGHGVILKVVSTNICGSDQHMVRGRTTAPVGLVLGHEITGEVVEVGRDV
ETLKIGDLVSVPFNVACGRCAMCKETHTGVCLNVNPSRAGGAYGYVDMGGWIGGQAEYVLVPYADFNLLKFPDRDQAMAK
IRDLTCLSDILPTGYHGAVSAGVKPGSTVYIAGAGPVGMAAAASARLLGAAVTIVGDMNAERLAHAKAMGFETVDLSKDA
TLGEQIAQILGKPEIDCAVDCVGFEAHGHGSSGHAEEAPATVLNSLMEITRPAGAIGIPGLYVTDDPGAQDKAAQHGSLS
IRFGLGWAKSHSFFTGQTPVLKYNRNLMQAILYDRLPIAKIVNVTVISLDDAPEGYKKFDGGAPRKFVIDPHGLLAA

Specific function: Catalyzes the oxidation of long-chain alcohols but is inactive against ethanol [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501933, Length=204, Percent_Identity=33.8235294117647, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4501929, Length=205, Percent_Identity=33.6585365853659, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI34577061, Length=204, Percent_Identity=32.843137254902, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI156627571, Length=279, Percent_Identity=30.1075268817204, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI71743840, Length=226, Percent_Identity=29.646017699115, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI262073058, Length=226, Percent_Identity=29.646017699115, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI71565154, Length=212, Percent_Identity=29.7169811320755, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI156523966, Length=204, Percent_Identity=28.4313725490196, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI4501939, Length=204, Percent_Identity=28.4313725490196, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786825, Length=407, Percent_Identity=33.4152334152334, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1790045, Length=235, Percent_Identity=33.6170212765957, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082125, Length=240, Percent_Identity=33.75, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790718, Length=269, Percent_Identity=26.0223048327138, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788073, Length=204, Percent_Identity=31.8627450980392, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI226510992, Length=224, Percent_Identity=32.5892857142857, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787863, Length=230, Percent_Identity=30.8695652173913, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081918, Length=205, Percent_Identity=34.6341463414634, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788075, Length=288, Percent_Identity=25.6944444444444, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=236, Percent_Identity=29.2372881355932, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562878, Length=245, Percent_Identity=27.3469387755102, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322619, Length=274, Percent_Identity=32.1167883211679, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319955, Length=274, Percent_Identity=32.1167883211679, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320033, Length=276, Percent_Identity=29.7101449275362, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=259, Percent_Identity=26.2548262548263, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137530, Length=245, Percent_Identity=31.4285714285714, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17737897, Length=279, Percent_Identity=29.0322580645161, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17737895, Length=221, Percent_Identity=30.316742081448, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR014184
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.2.1.46 [H]

Molecular weight: Translated: 41692; Mature: 41561

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNRGVVYLGPGKVEVQKIDYPKMVDPSGRAIGHGVILKVVSTNICGSDQHMVRGRTTA
CCCCCCEEEECCCEEEEEECCCCCCCCCCCCEECCEEEEEEEECCCCCCCCCEECCCCCC
PVGLVLGHEITGEVVEVGRDVETLKIGDLVSVPFNVACGRCAMCKETHTGVCLNVNPSRA
CEEEEECCCCCHHHHHHCCCEEEEEECCEEECCCCCCCCCCHHCCCCCCCEEEEECCCCC
GGAYGYVDMGGWIGGQAEYVLVPYADFNLLKFPDRDQAMAKIRDLTCLSDILPTGYHGAV
CCCEEEEECCCCCCCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCE
SAGVKPGSTVYIAGAGPVGMAAAASARLLGAAVTIVGDMNAERLAHAKAMGFETVDLSKD
ECCCCCCCEEEEEECCCCCHHHHHHHHHHEEEEEEEECCCHHHHHHHHHCCCEEEECCCC
ATLGEQIAQILGKPEIDCAVDCVGFEAHGHGSSGHAEEAPATVLNSLMEITRPAGAIGIP
CHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GLYVTDDPGAQDKAAQHGSLSIRFGLGWAKSHSFFTGQTPVLKYNRNLMQAILYDRLPIA
EEEEECCCCCCCHHCCCCCEEEEEECCCCCCCCEECCCCCEEEHHHHHHHHHHHCCCCHH
KIVNVTVISLDDAPEGYKKFDGGAPRKFVIDPHGLLAA
HEEEEEEEEECCCCCHHHHCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SSNRGVVYLGPGKVEVQKIDYPKMVDPSGRAIGHGVILKVVSTNICGSDQHMVRGRTTA
CCCCCEEEECCCEEEEEECCCCCCCCCCCCEECCEEEEEEEECCCCCCCCCEECCCCCC
PVGLVLGHEITGEVVEVGRDVETLKIGDLVSVPFNVACGRCAMCKETHTGVCLNVNPSRA
CEEEEECCCCCHHHHHHCCCEEEEEECCEEECCCCCCCCCCHHCCCCCCCEEEEECCCCC
GGAYGYVDMGGWIGGQAEYVLVPYADFNLLKFPDRDQAMAKIRDLTCLSDILPTGYHGAV
CCCEEEEECCCCCCCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCE
SAGVKPGSTVYIAGAGPVGMAAAASARLLGAAVTIVGDMNAERLAHAKAMGFETVDLSKD
ECCCCCCCEEEEEECCCCCHHHHHHHHHHEEEEEEEECCCHHHHHHHHHCCCEEEECCCC
ATLGEQIAQILGKPEIDCAVDCVGFEAHGHGSSGHAEEAPATVLNSLMEITRPAGAIGIP
CHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GLYVTDDPGAQDKAAQHGSLSIRFGLGWAKSHSFFTGQTPVLKYNRNLMQAILYDRLPIA
EEEEECCCCCCCHHCCCCCEEEEEECCCCCCCCEECCCCCEEEHHHHHHHHHHHCCCCHH
KIVNVTVISLDDAPEGYKKFDGGAPRKFVIDPHGLLAA
HEEEEEEEEECCCCCHHHHCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]