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The map label for this gene is gcvA [H]

Identifier: 189353732

GI number: 189353732

Start: 2001812

End: 2002744

Strand: Reverse

Name: gcvA [H]

Synonym: BMULJ_04977

Alternate gene names: 189353732

Gene position: 2002744-2001812 (Counterclockwise)

Preceding gene: 189353733

Following gene: 189353728

Centisome position: 80.98

GC content: 69.67

Gene sequence:

>933_bases
ATGGCCAAGTCGATTCCCCTCGCAGCGCTGCACACGTTCGTCGAAGTCGCGCGCTGCGGCAGCATGAAGCAGGCGGCCGA
TCTGCTCTGCCTGTCGCCCGGCGCGGTGAGTCAGCACGTGCGCCATCTCGAGGATCGCCTCGCGGTGCGGCTGTTCGAAC
GCAACGGCCGTGAACTCGAGCTGACTGGCACCGGCCGCGCGCTGTTCGACGCACTCGCCGGCGGTTTCCGCGAAATCGAA
AGCGCGTGGGCAAACCTGCACGGCGCGAACCGGCGCGTGGTGCGCCTGACCGTGACGACGACCGCGTCGTTCGCGAACAC
CTGGCTCGTCCAGCGTCTCGGCGCGTTCAACGCGCACTATCCGGAAATCGAAATCACGATCGACACGCGGCCGCAACTGG
TCGACCTGCGCCGCGACTATGTCGACGTCGCGATCCGGCACGGGCTCGGCCAATATCCGGGACACGCGTCGTTCCGCATC
TGGACGCCCGAGCTGTGGCCGGTATGCAGCCCGCAACTGCTCGATCCGGCGCGGCCGATCGCGCAGCCGGCCGACTGTCT
CGCGTATCCGCTGCTGCAGGACGCGGACCGCGCGGACTGGGTGCTGTGGCTGCGCGCGCAGGGCATCGAGGATCCGCGCG
CGCAGCGCGGCGCGAGCTTCAGCGACGACGCGCTGCTGATCAAGGCCGCCGTCGCGTCGCAGGGCATCGCGCTCGTGCGC
GACATCTATGCGCGCGACGAAATCGCGGCCGGGCGCCTCGTACGCGTGCTCGCGCGGCCGTGGCCGACGGAGCTGTCGTA
TTACGTCGTCTGCAACGAAGCGCGTCGCGACGAGTGGAAGATCGTCGCGTTTCGCGACTGGCTCGCGCGCGAAATCGGCG
ACGGCGCCGGCGAGCCGGCAGGCCGCGATACGCGCGACACGCTAGCGCCGTGA

Upstream 100 bases:

>100_bases
CGGCTGCGCGTTTTTCCGCGCGCCATCGCGCGCGCGATTGCTGCGATAATCGACGCCCAGTTCCTGAACCTTCTCGCGGC
AGCCCGCAGCCGATACCCGG

Downstream 100 bases:

>100_bases
CGCGCGCGCGGCCGCGGTCGCGAACCCGCTGAGCTTGCCGGTCGCGAGCGCGGTGCCGAGCAGGATGATCGCGCAGCCGG
CGAGCGTCTGCGCGGTGATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MAKSIPLAALHTFVEVARCGSMKQAADLLCLSPGAVSQHVRHLEDRLAVRLFERNGRELELTGTGRALFDALAGGFREIE
SAWANLHGANRRVVRLTVTTTASFANTWLVQRLGAFNAHYPEIEITIDTRPQLVDLRRDYVDVAIRHGLGQYPGHASFRI
WTPELWPVCSPQLLDPARPIAQPADCLAYPLLQDADRADWVLWLRAQGIEDPRAQRGASFSDDALLIKAAVASQGIALVR
DIYARDEIAAGRLVRVLARPWPTELSYYVVCNEARRDEWKIVAFRDWLAREIGDGAGEPAGRDTRDTLAP

Sequences:

>Translated_310_residues
MAKSIPLAALHTFVEVARCGSMKQAADLLCLSPGAVSQHVRHLEDRLAVRLFERNGRELELTGTGRALFDALAGGFREIE
SAWANLHGANRRVVRLTVTTTASFANTWLVQRLGAFNAHYPEIEITIDTRPQLVDLRRDYVDVAIRHGLGQYPGHASFRI
WTPELWPVCSPQLLDPARPIAQPADCLAYPLLQDADRADWVLWLRAQGIEDPRAQRGASFSDDALLIKAAVASQGIALVR
DIYARDEIAAGRLVRVLARPWPTELSYYVVCNEARRDEWKIVAFRDWLAREIGDGAGEPAGRDTRDTLAP
>Mature_309_residues
AKSIPLAALHTFVEVARCGSMKQAADLLCLSPGAVSQHVRHLEDRLAVRLFERNGRELELTGTGRALFDALAGGFREIES
AWANLHGANRRVVRLTVTTTASFANTWLVQRLGAFNAHYPEIEITIDTRPQLVDLRRDYVDVAIRHGLGQYPGHASFRIW
TPELWPVCSPQLLDPARPIAQPADCLAYPLLQDADRADWVLWLRAQGIEDPRAQRGASFSDDALLIKAAVASQGIALVRD
IYARDEIAAGRLVRVLARPWPTELSYYVVCNEARRDEWKIVAFRDWLAREIGDGAGEPAGRDTRDTLAP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=37.3702422145329, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=32.6530612244898, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=28.2312925170068, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786401, Length=306, Percent_Identity=26.797385620915, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=24, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787128, Length=148, Percent_Identity=32.4324324324324, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789639, Length=303, Percent_Identity=26.0726072607261, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=26.7558528428094, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=273, Percent_Identity=27.1062271062271, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34507; Mature: 34375

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSIPLAALHTFVEVARCGSMKQAADLLCLSPGAVSQHVRHLEDRLAVRLFERNGRELE
CCCCCHHHHHHHHHHHHHCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LTGTGRALFDALAGGFREIESAWANLHGANRRVVRLTVTTTASFANTWLVQRLGAFNAHY
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHHHHCCCCCC
PEIEITIDTRPQLVDLRRDYVDVAIRHGLGQYPGHASFRIWTPELWPVCSPQLLDPARPI
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
AQPADCLAYPLLQDADRADWVLWLRAQGIEDPRAQRGASFSDDALLIKAAVASQGIALVR
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCCCHHEEEHHHHHCCHHHHH
DIYARDEIAAGRLVRVLARPWPTELSYYVVCNEARRDEWKIVAFRDWLAREIGDGAGEPA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCCCC
GRDTRDTLAP
CCCCHHCCCC
>Mature Secondary Structure 
AKSIPLAALHTFVEVARCGSMKQAADLLCLSPGAVSQHVRHLEDRLAVRLFERNGRELE
CCCCHHHHHHHHHHHHHCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LTGTGRALFDALAGGFREIESAWANLHGANRRVVRLTVTTTASFANTWLVQRLGAFNAHY
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHHHHCCCCCC
PEIEITIDTRPQLVDLRRDYVDVAIRHGLGQYPGHASFRIWTPELWPVCSPQLLDPARPI
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
AQPADCLAYPLLQDADRADWVLWLRAQGIEDPRAQRGASFSDDALLIKAAVASQGIALVR
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCCCHHEEEHHHHHCCHHHHH
DIYARDEIAAGRLVRVLARPWPTELSYYVVCNEARRDEWKIVAFRDWLAREIGDGAGEPA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCCCC
GRDTRDTLAP
CCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]