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The map label for this gene is purU [H]

Identifier: 189353707

GI number: 189353707

Start: 1971213

End: 1972097

Strand: Direct

Name: purU [H]

Synonym: BMULJ_04952

Alternate gene names: 189353707

Gene position: 1971213-1972097 (Clockwise)

Preceding gene: 189353703

Following gene: 189353714

Centisome position: 79.7

GC content: 64.41

Gene sequence:

>885_bases
ATGACCGCCGACTCACGCCCCGATCAATTCGTCTTGACGCTGTCGTGCCCGAGCGCGGCCGGCCAGGTAGCCGCCGTCGT
CGGCTTTCTCGAGCGCCATCGCTGCTACGTCGATGCGCTGAACGTGTTCGACGACGATCTCAGCAACCATTTCTTCGTGC
GCTGCGTGTTTCATCCGACGGATGAAACCCTTCAGATCGACGCCCTGCGACAGGAGTTCGGGCCGATCGCGGCTTCGCTC
GCGGGCGGCGCAGGCGACACGCAATGGGCCATCCACGACGTCAACGCGCGCCCGAAGGTGCTGATCATGGTGTCGAAGCT
CGAGCACTGCCTCGCGGATCTGCTGTTCCGCTGGCGGATGGGCGAGCTGAAGATGGACATCGTCGGCATCGTGTCCAACC
ATCCGGACTTCGAACCGCTCGCCGCGCAGCACGGGCTACCGTTCCGCCACTTCCCGATCACGCCCGACACGAAGGCGCAG
CAGGAAGCGCAGTGGCTCGACTTCTTCGAGACGAGCGGCGCCGAGCTCGTGATCCTCGCGCGCTACATGCAGGTGCTGTC
GCAGGAGACGAGCGCGAAGCTCGCGAACCGCGCGATCAACATCCATCATTCGTTCCTGCCGGGTTTCAAGGGCGCGAAGC
CGTATCACCAGGCGCATGCGCGCGGCGTGAAGCTGATCGGCGCGACCGCGCACTTCGTGACCGACGATCTCGACGAAGGC
CCGATCATCGAGCAGGTCGTCGAGCGCGTCGATCATGCGTTGCGGCCGGAGCAGCTGCTCGCGGTCGGACGCGATGTCGA
ATGCATCACGCTCGCGCGGGCCGTGAAGGCGTTTATCGAGCGAAGGGTGTTTTTGAACGGGGATCGGACGGTGGTGTTTT
CCTGA

Upstream 100 bases:

>100_bases
AAATCGTCCTGATTAACTTGAGGCTCCCGCGGCCCGTGTCGCGCGGCGCCGTTGTGCGTCGCCGCACGGATTTCGCGCTC
CATGACATTCGAGGAGTTTC

Downstream 100 bases:

>100_bases
ACGGATCGCGCGCGGCAGGTTCGCGAACCGCCGCGCGCGCCCGACGCACGGCTCGCGTCAGCCGTTACGAACGCGAAAGT
CGGCCGGCGCGCTGTGCGAC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTADSRPDQFVLTLSCPSAAGQVAAVVGFLERHRCYVDALNVFDDDLSNHFFVRCVFHPTDETLQIDALRQEFGPIAASL
AGGAGDTQWAIHDVNARPKVLIMVSKLEHCLADLLFRWRMGELKMDIVGIVSNHPDFEPLAAQHGLPFRHFPITPDTKAQ
QEAQWLDFFETSGAELVILARYMQVLSQETSAKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEG
PIIEQVVERVDHALRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFS

Sequences:

>Translated_294_residues
MTADSRPDQFVLTLSCPSAAGQVAAVVGFLERHRCYVDALNVFDDDLSNHFFVRCVFHPTDETLQIDALRQEFGPIAASL
AGGAGDTQWAIHDVNARPKVLIMVSKLEHCLADLLFRWRMGELKMDIVGIVSNHPDFEPLAAQHGLPFRHFPITPDTKAQ
QEAQWLDFFETSGAELVILARYMQVLSQETSAKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEG
PIIEQVVERVDHALRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFS
>Mature_293_residues
TADSRPDQFVLTLSCPSAAGQVAAVVGFLERHRCYVDALNVFDDDLSNHFFVRCVFHPTDETLQIDALRQEFGPIAASLA
GGAGDTQWAIHDVNARPKVLIMVSKLEHCLADLLFRWRMGELKMDIVGIVSNHPDFEPLAAQHGLPFRHFPITPDTKAQQ
EAQWLDFFETSGAELVILARYMQVLSQETSAKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGP
IIEQVVERVDHALRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFS

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1787483, Length=287, Percent_Identity=36.9337979094077, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1788846, Length=150, Percent_Identity=27.3333333333333, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17567511, Length=190, Percent_Identity=35.2631578947368, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32742; Mature: 32611

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADSRPDQFVLTLSCPSAAGQVAAVVGFLERHRCYVDALNVFDDDLSNHFFVRCVFHPT
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DETLQIDALRQEFGPIAASLAGGAGDTQWAIHDVNARPKVLIMVSKLEHCLADLLFRWRM
CCCEEHHHHHHHHCHHHHHHCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHC
GELKMDIVGIVSNHPDFEPLAAQHGLPFRHFPITPDTKAQQEAQWLDFFETSGAELVILA
CCEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
RYMQVLSQETSAKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEG
HHHHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHCCCCCCC
PIIEQVVERVDHALRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFS
CHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCEEEEEC
>Mature Secondary Structure 
TADSRPDQFVLTLSCPSAAGQVAAVVGFLERHRCYVDALNVFDDDLSNHFFVRCVFHPT
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DETLQIDALRQEFGPIAASLAGGAGDTQWAIHDVNARPKVLIMVSKLEHCLADLLFRWRM
CCCEEHHHHHHHHCHHHHHHCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHC
GELKMDIVGIVSNHPDFEPLAAQHGLPFRHFPITPDTKAQQEAQWLDFFETSGAELVILA
CCEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
RYMQVLSQETSAKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEG
HHHHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHCCCCCCC
PIIEQVVERVDHALRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVFS
CHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]