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The map label for this gene is gcvA [H]

Identifier: 189353703

GI number: 189353703

Start: 1967027

End: 1967959

Strand: Direct

Name: gcvA [H]

Synonym: BMULJ_04948

Alternate gene names: 189353703

Gene position: 1967027-1967959 (Clockwise)

Preceding gene: 189353695

Following gene: 189353707

Centisome position: 79.53

GC content: 72.13

Gene sequence:

>933_bases
ATGTCGAAACCGGAACCGTTACCGTCGATGCAGGCGTTGCGCGCGTTCGAGTCGGCCGCGCGGCTCGCGAGCTTCACGGC
CGCTGCGCGCGAACTCGGCTCCACGCAGCCGGCCGTCAGCCAGCAGGTGTTTCAGCTCGAGGCCGAGCTCGGCGTGCCGC
TGTTCGAGCGCAGCCCGCGCGGCGTCACGCTGACGGCCGACGGCGAATGCCTGTACGAGGCCGTCCGGCTCAGCCTCGAC
ACCTTGCGCGGCGCGACCGCGACACTGCGCGCGCGCCGCGAGCACGGCACGCTGACGATCGTCACCGACTTCGGTTTCGC
GACGTACTGGCTGATGCCGCGCCTTGCCGGGCTCAAGCGCGTGATGCCCGACGTGGACGTGCGCGTGGTCACGTCGCAGG
ACTACGACGCGCAGCGCGACCATGCCGACATCGCGATCCTGTTCGGCGACGGCCACTGGCCGTCGTGCACGGCCGCGCGG
CTGTTTGCGGAGTCGGTCACGCCGGTCTGCTCGCCGGCGTTCCGCGATGCGCATCCGCACGTCTCGCGGCCCGAGCATCT
GGCTGCGCTGCCGTTGCTGCACGTGCAGCCGACGCGCCCCGAGCGCTGGCTGTCGTGGGCCGGCTGGTTCGACGCGCACG
GTCTCGATGCGCAGGCCGCGGCGCGCGGCGTGACATTCAACAGCTACGCGCTCGTGATCCATGCGGCGCTGCTCGGCGAA
GGCGTGGCGCTCGGCTGGTCGCCGCTCGTCGACGAGCTTGTCGCGGCCGGCCAGCTCGTGAAGCTCGTCGACGCGCCGGT
CGTCACGTCGCGCGGTTATTTTCTGGTGCGGCCGCCGCAGCGGCCGGAGCCGGACGCGACGCATGTGTTCCGGCGGTGGC
TGCTCGATGCGTGCGCGTGTGCGGGGGCGGGCGGCGGACAGGCCAGCGGATAG

Upstream 100 bases:

>100_bases
AGGCCTTGCGCGGCGCGGTGCCGCGCCGTCCGATGCACTATCTCCCGTCCGAATTCGCGCGGGAAGGCGTCCGGCTCTTA
TGGGGCCATTAGAGGGACTT

Downstream 100 bases:

>100_bases
CCCGCCTGAGTGGGCTGGTCCGGGCGCGCCCGCCGTGCCGCGTCGGCGCTTGGCACGCGGATGAAACCACCGCTCGATCA
CAGGCGATCGCACCGTCATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSKPEPLPSMQALRAFESAARLASFTAAARELGSTQPAVSQQVFQLEAELGVPLFERSPRGVTLTADGECLYEAVRLSLD
TLRGATATLRARREHGTLTIVTDFGFATYWLMPRLAGLKRVMPDVDVRVVTSQDYDAQRDHADIAILFGDGHWPSCTAAR
LFAESVTPVCSPAFRDAHPHVSRPEHLAALPLLHVQPTRPERWLSWAGWFDAHGLDAQAAARGVTFNSYALVIHAALLGE
GVALGWSPLVDELVAAGQLVKLVDAPVVTSRGYFLVRPPQRPEPDATHVFRRWLLDACACAGAGGGQASG

Sequences:

>Translated_310_residues
MSKPEPLPSMQALRAFESAARLASFTAAARELGSTQPAVSQQVFQLEAELGVPLFERSPRGVTLTADGECLYEAVRLSLD
TLRGATATLRARREHGTLTIVTDFGFATYWLMPRLAGLKRVMPDVDVRVVTSQDYDAQRDHADIAILFGDGHWPSCTAAR
LFAESVTPVCSPAFRDAHPHVSRPEHLAALPLLHVQPTRPERWLSWAGWFDAHGLDAQAAARGVTFNSYALVIHAALLGE
GVALGWSPLVDELVAAGQLVKLVDAPVVTSRGYFLVRPPQRPEPDATHVFRRWLLDACACAGAGGGQASG
>Mature_309_residues
SKPEPLPSMQALRAFESAARLASFTAAARELGSTQPAVSQQVFQLEAELGVPLFERSPRGVTLTADGECLYEAVRLSLDT
LRGATATLRARREHGTLTIVTDFGFATYWLMPRLAGLKRVMPDVDVRVVTSQDYDAQRDHADIAILFGDGHWPSCTAARL
FAESVTPVCSPAFRDAHPHVSRPEHLAALPLLHVQPTRPERWLSWAGWFDAHGLDAQAAARGVTFNSYALVIHAALLGEG
VALGWSPLVDELVAAGQLVKLVDAPVVTSRGYFLVRPPQRPEPDATHVFRRWLLDACACAGAGGGQASG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=36, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=33.9041095890411, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87081978, Length=262, Percent_Identity=30.5343511450382, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=32.1212121212121, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786401, Length=262, Percent_Identity=27.0992366412214, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789440, Length=279, Percent_Identity=25.4480286738351, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788887, Length=178, Percent_Identity=33.1460674157303, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=129, Percent_Identity=34.8837209302326, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=125, Percent_Identity=35.2, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=25.4180602006689, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=301, Percent_Identity=26.9102990033223, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33506; Mature: 33375

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPEPLPSMQALRAFESAARLASFTAAARELGSTQPAVSQQVFQLEAELGVPLFERSPR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC
GVTLTADGECLYEAVRLSLDTLRGATATLRARREHGTLTIVTDFGFATYWLMPRLAGLKR
CEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
VMPDVDVRVVTSQDYDAQRDHADIAILFGDGHWPSCTAARLFAESVTPVCSPAFRDAHPH
HCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHCCCHHHCCCCC
VSRPEHLAALPLLHVQPTRPERWLSWAGWFDAHGLDAQAAARGVTFNSYALVIHAALLGE
CCCCCHHHHCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHC
GVALGWSPLVDELVAAGQLVKLVDAPVVTSRGYFLVRPPQRPEPDATHVFRRWLLDACAC
CCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
AGAGGGQASG
CCCCCCCCCC
>Mature Secondary Structure 
SKPEPLPSMQALRAFESAARLASFTAAARELGSTQPAVSQQVFQLEAELGVPLFERSPR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC
GVTLTADGECLYEAVRLSLDTLRGATATLRARREHGTLTIVTDFGFATYWLMPRLAGLKR
CEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
VMPDVDVRVVTSQDYDAQRDHADIAILFGDGHWPSCTAARLFAESVTPVCSPAFRDAHPH
HCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHCCCHHHCCCCC
VSRPEHLAALPLLHVQPTRPERWLSWAGWFDAHGLDAQAAARGVTFNSYALVIHAALLGE
CCCCCHHHHCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHC
GVALGWSPLVDELVAAGQLVKLVDAPVVTSRGYFLVRPPQRPEPDATHVFRRWLLDACAC
CCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
AGAGGGQASG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]