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The map label for this gene is yitF [H]

Identifier: 189353675

GI number: 189353675

Start: 1938156

End: 1939433

Strand: Reverse

Name: yitF [H]

Synonym: BMULJ_04920

Alternate gene names: 189353675

Gene position: 1939433-1938156 (Counterclockwise)

Preceding gene: 189353676

Following gene: 189353674

Centisome position: 78.42

GC content: 67.14

Gene sequence:

>1278_bases
ATGACGCTCATTCGATCGATACGCGTGCTCGACGTGCGCTTTCCGACCTCGCGCCAGCTCGACGGCTCCGACGCGATGAA
TCCCGATCCCGACTACTCGGCTGCCTACGTCGTGCTCGAAACCGACCGCGACGGGCTCGAAGGGCACGGGCTCACGTTCA
CGATCGGGCGCGGCAACGAGATCTGCTGCGCGGCGATCGCCGCGATGCGGCATCTCGTCGTCGGCCTCGATCTCGACTGG
ATCCGCGAGGACATGGGCCGCTTCTGGCGGCATGTGACGTCGGACAGCCAGCTGCGCTGGATCGGCCCCGACAAGGGCGC
GATTCATCTCGCGACCGGCGCGGTCGTCAACGCGGTGTGGGACTTGTGGGCGAAGGTCGAAGGCAAGCCGCTCTGGCGGC
TCGTCGCCGACATGAGCCCCGAGCAGCTCGTGCGTGCGATCGACTTCCGCTATCTGACCGACTGCCTGACGCCGGACGAG
GCGCTCGACCTGCTGCGGCGCCAGGCGCCCGGCAAGGCCGACCGGATCGCAGTGCTCGAGCGAGAAGGCTATCCGTGCTA
CACGACCTCCGCGGGCTGGCTCGGCTACAGCGACGACAAGCTGCAGCGGCTCTGCCGCGAAGCGGTCGACGCGGGCTTCG
ATCACGTGAAGCTGAAGGTCGGCGCGAATCTCGACGACGACATTCGCCGCGTGTCGATCGCGCGCGACGTGATCGGCCCG
GACCGCAAGCTGATGATCGACGCGAACCAGGTGTGGGAAGTCGGCGAAGCGATCGACTGGGTGCGTGCGCTCGCGTTCGC
GCGGCCGTGGTTCATCGAGGAGCCGACGAGCCCCGACGACGTCGAAGGGCATCGCAAGATCCGCGAGGCGATCGCGCCGG
TGCAGGTCGCGACCGGCGAAATGTGCCAGAACCGCGTGCTGTTCAAGCAGTTCATCGAGCGTCGCGCGATCGACGTCGTG
CAGATCGACGCGTGCCGGCTCGGCGGCGTGAACGAGATTCTCGCGGTGATGCTGATGGCCGCGAAGTACGGGCTGCCCGT
GTGCCCGCATGCGGGCGGCGTCGGGCTCTGCGAGTACGTGCAGCACCTGTCGATGATCGACTACGTATGCATCGCGGGCA
CGAAGGAAGGGCGCGTGACCGAGTACGTCGATCATCTGCACGAGCATTTCGTCGAACCGTGCATCGTGCGGGACGCGGCG
TATCTGCCGCCGAGCGCGCCGGGCTTCTCGATCGAGATGAAGCCCGCCTCGCTCGAGCAATACCGGTTCCGCGGCTGA

Upstream 100 bases:

>100_bases
TATCTGCGTGCCGGGCAGGTGATGACGCTCGGCATCGACGGGCTGGGCGAGCAGCGCCAGCGCACGGTACAGGCCTGATC
TTTTTCGCGGACGGTTCCTC

Downstream 100 bases:

>100_bases
CGCAACTGCGGCCGACAGACGGCCTGCGCGGCCGCGACATGAACGACGGAGACGCGAAGTGGATCTGAATCTGCAGGACA
AGGTCGTGATCGTGACGGGC

Product: mandelate racemase

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTLIRSIRVLDVRFPTSRQLDGSDAMNPDPDYSAAYVVLETDRDGLEGHGLTFTIGRGNEICCAAIAAMRHLVVGLDLDW
IREDMGRFWRHVTSDSQLRWIGPDKGAIHLATGAVVNAVWDLWAKVEGKPLWRLVADMSPEQLVRAIDFRYLTDCLTPDE
ALDLLRRQAPGKADRIAVLEREGYPCYTTSAGWLGYSDDKLQRLCREAVDAGFDHVKLKVGANLDDDIRRVSIARDVIGP
DRKLMIDANQVWEVGEAIDWVRALAFARPWFIEEPTSPDDVEGHRKIREAIAPVQVATGEMCQNRVLFKQFIERRAIDVV
QIDACRLGGVNEILAVMLMAAKYGLPVCPHAGGVGLCEYVQHLSMIDYVCIAGTKEGRVTEYVDHLHEHFVEPCIVRDAA
YLPPSAPGFSIEMKPASLEQYRFRG

Sequences:

>Translated_425_residues
MTLIRSIRVLDVRFPTSRQLDGSDAMNPDPDYSAAYVVLETDRDGLEGHGLTFTIGRGNEICCAAIAAMRHLVVGLDLDW
IREDMGRFWRHVTSDSQLRWIGPDKGAIHLATGAVVNAVWDLWAKVEGKPLWRLVADMSPEQLVRAIDFRYLTDCLTPDE
ALDLLRRQAPGKADRIAVLEREGYPCYTTSAGWLGYSDDKLQRLCREAVDAGFDHVKLKVGANLDDDIRRVSIARDVIGP
DRKLMIDANQVWEVGEAIDWVRALAFARPWFIEEPTSPDDVEGHRKIREAIAPVQVATGEMCQNRVLFKQFIERRAIDVV
QIDACRLGGVNEILAVMLMAAKYGLPVCPHAGGVGLCEYVQHLSMIDYVCIAGTKEGRVTEYVDHLHEHFVEPCIVRDAA
YLPPSAPGFSIEMKPASLEQYRFRG
>Mature_424_residues
TLIRSIRVLDVRFPTSRQLDGSDAMNPDPDYSAAYVVLETDRDGLEGHGLTFTIGRGNEICCAAIAAMRHLVVGLDLDWI
REDMGRFWRHVTSDSQLRWIGPDKGAIHLATGAVVNAVWDLWAKVEGKPLWRLVADMSPEQLVRAIDFRYLTDCLTPDEA
LDLLRRQAPGKADRIAVLEREGYPCYTTSAGWLGYSDDKLQRLCREAVDAGFDHVKLKVGANLDDDIRRVSIARDVIGPD
RKLMIDANQVWEVGEAIDWVRALAFARPWFIEEPTSPDDVEGHRKIREAIAPVQVATGEMCQNRVLFKQFIERRAIDVVQ
IDACRLGGVNEILAVMLMAAKYGLPVCPHAGGVGLCEYVQHLSMIDYVCIAGTKEGRVTEYVDHLHEHFVEPCIVRDAAY
LPPSAPGFSIEMKPASLEQYRFRG

Specific function: D-galactonate degradation; first step. D-galactonate degradation; third step. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=423, Percent_Identity=55.3191489361702, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI42544117, Length=403, Percent_Identity=54.3424317617866, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI186972148, Length=331, Percent_Identity=54.9848942598187, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 47614; Mature: 47483

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIRSIRVLDVRFPTSRQLDGSDAMNPDPDYSAAYVVLETDRDGLEGHGLTFTIGRGNE
CCHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCH
ICCAAIAAMRHLVVGLDLDWIREDMGRFWRHVTSDSQLRWIGPDKGAIHLATGAVVNAVW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHHHH
DLWAKVEGKPLWRLVADMSPEQLVRAIDFRYLTDCLTPDEALDLLRRQAPGKADRIAVLE
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEE
REGYPCYTTSAGWLGYSDDKLQRLCREAVDAGFDHVKLKVGANLDDDIRRVSIARDVIGP
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
DRKLMIDANQVWEVGEAIDWVRALAFARPWFIEEPTSPDDVEGHRKIREAIAPVQVATGE
CCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
MCQNRVLFKQFIERRAIDVVQIDACRLGGVNEILAVMLMAAKYGLPVCPHAGGVGLCEYV
HHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
QHLSMIDYVCIAGTKEGRVTEYVDHLHEHFVEPCIVRDAAYLPPSAPGFSIEMKPASLEQ
HHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEECCCCHHH
YRFRG
HCCCC
>Mature Secondary Structure 
TLIRSIRVLDVRFPTSRQLDGSDAMNPDPDYSAAYVVLETDRDGLEGHGLTFTIGRGNE
CHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCH
ICCAAIAAMRHLVVGLDLDWIREDMGRFWRHVTSDSQLRWIGPDKGAIHLATGAVVNAVW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHHHH
DLWAKVEGKPLWRLVADMSPEQLVRAIDFRYLTDCLTPDEALDLLRRQAPGKADRIAVLE
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEE
REGYPCYTTSAGWLGYSDDKLQRLCREAVDAGFDHVKLKVGANLDDDIRRVSIARDVIGP
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
DRKLMIDANQVWEVGEAIDWVRALAFARPWFIEEPTSPDDVEGHRKIREAIAPVQVATGE
CCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
MCQNRVLFKQFIERRAIDVVQIDACRLGGVNEILAVMLMAAKYGLPVCPHAGGVGLCEYV
HHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
QHLSMIDYVCIAGTKEGRVTEYVDHLHEHFVEPCIVRDAAYLPPSAPGFSIEMKPASLEQ
HHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEECCCCHHH
YRFRG
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]