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The map label for this gene is ampR [H]

Identifier: 189353659

GI number: 189353659

Start: 1920140

End: 1921033

Strand: Direct

Name: ampR [H]

Synonym: BMULJ_04904

Alternate gene names: 189353659

Gene position: 1920140-1921033 (Clockwise)

Preceding gene: 189353651

Following gene: 189353661

Centisome position: 77.64

GC content: 71.03

Gene sequence:

>894_bases
ATGCTCAAGCGATACCCTTCGATCCAGTCGATGCAGGCATTTCTGCAGGCCGCGCGGGTCGGCAGCTTCTCCAGCGCCGC
ACGGCAGCTCGCGCTGACACACAGCGCGATCAGTCAGCAGATCCGCTCGCTCGAGGAATTCATCGGCCAGCCGCTGTTCG
TGCGCACGGGCGGCCGCGTGAGCCTTACCGATGCCGGCACGCTGCTCGCCAACCAGTTGTCGGACGGCCTCGAGCAGATC
GATCGCGCGCTGTCGTCCGTGAAGGGCCGCGCGAGCGCGCCGTCGCTGCGGCTCGACGTCGACCCCGAACTGATGCAGGG
CTGGCTGCCGGCGCGCATGCCGGCACTGATGCGCGCGCTCGGCGGCACGACGCTGACGGTGCTGTCGGCGCCGCGCCACG
ATCGCGATGCGTTCGATCGCGTCGATGTCGCGCTGCGCTACGGCTACGGCGAATGGGAAGGCGTCGACAGCGCGCTGGTC
TGCCCGGATCGGCTGACCGCGATGGCCTCGCCGGCGCTGCTCGAACGCTACGGGTTGCGCGCGCCGCTCGCACCGGCCAG
CGTGCTGACACTGCCGCTGCTCGGCTATACGAAACGCTCGTGGATTCCTTGGCTCGAGGCGGCCGGACTCGCGCCGGTCG
AACCCGACACGATCGCGGTGTTCGACAACGCGGCCGGGCTCGTCGCCGCGCTCGCCTCAGGCCTCGGCGCGGGCCTCGCG
CGCGGGCTGCTCGCCGCCGACGCTCGCCGCGACGGCCGGCTCGTCGAACTGTGCTCGATCGCGATTCCGACGCACTACAA
CCTCCATGCGATCTGGCCGCGCGAGCGGCACGCGCGCGTGAAGCCGCTGCTCGATGCGATCGGCGCGCTGGTCGCGCAAA
CGCTCGCGCGCTGA

Upstream 100 bases:

>100_bases
TGGGGTCGCGCATCGCCGTGCGCGGTATCGCATTATCGAAACGCGGCGCGCTCGCGCGCCAGCGATCATTTCGTCGATTC
CCTGTCAGTCGGACTAACAG

Downstream 100 bases:

>100_bases
GTCACGCACGGTATTCGGCCGCGACTTCGCGCACCGTATCGAGAAACGCGCCGAGCACGGCGGACGTGTCGTCCGCGCGG
TAGCGCGCCTGCAGCGACAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLKRYPSIQSMQAFLQAARVGSFSSAARQLALTHSAISQQIRSLEEFIGQPLFVRTGGRVSLTDAGTLLANQLSDGLEQI
DRALSSVKGRASAPSLRLDVDPELMQGWLPARMPALMRALGGTTLTVLSAPRHDRDAFDRVDVALRYGYGEWEGVDSALV
CPDRLTAMASPALLERYGLRAPLAPASVLTLPLLGYTKRSWIPWLEAAGLAPVEPDTIAVFDNAAGLVAALASGLGAGLA
RGLLAADARRDGRLVELCSIAIPTHYNLHAIWPRERHARVKPLLDAIGALVAQTLAR

Sequences:

>Translated_297_residues
MLKRYPSIQSMQAFLQAARVGSFSSAARQLALTHSAISQQIRSLEEFIGQPLFVRTGGRVSLTDAGTLLANQLSDGLEQI
DRALSSVKGRASAPSLRLDVDPELMQGWLPARMPALMRALGGTTLTVLSAPRHDRDAFDRVDVALRYGYGEWEGVDSALV
CPDRLTAMASPALLERYGLRAPLAPASVLTLPLLGYTKRSWIPWLEAAGLAPVEPDTIAVFDNAAGLVAALASGLGAGLA
RGLLAADARRDGRLVELCSIAIPTHYNLHAIWPRERHARVKPLLDAIGALVAQTLAR
>Mature_297_residues
MLKRYPSIQSMQAFLQAARVGSFSSAARQLALTHSAISQQIRSLEEFIGQPLFVRTGGRVSLTDAGTLLANQLSDGLEQI
DRALSSVKGRASAPSLRLDVDPELMQGWLPARMPALMRALGGTTLTVLSAPRHDRDAFDRVDVALRYGYGEWEGVDSALV
CPDRLTAMASPALLERYGLRAPLAPASVLTLPLLGYTKRSWIPWLEAAGLAPVEPDTIAVFDNAAGLVAALASGLGAGLA
RGLLAADARRDGRLVELCSIAIPTHYNLHAIWPRERHARVKPLLDAIGALVAQTLAR

Specific function: Regulates the expression of the beta-lactamase gene. Represses cephalosporinase (AmpC) in the presence of beta-lactams and induces it in the absence of them [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=256, Percent_Identity=29.296875, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786448, Length=272, Percent_Identity=30.5147058823529, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=26.7361111111111, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788706, Length=283, Percent_Identity=25.0883392226148, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31842; Mature: 31842

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRYPSIQSMQAFLQAARVGSFSSAARQLALTHSAISQQIRSLEEFIGQPLFVRTGGRV
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
SLTDAGTLLANQLSDGLEQIDRALSSVKGRASAPSLRLDVDPELMQGWLPARMPALMRAL
EECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHH
GGTTLTVLSAPRHDRDAFDRVDVALRYGYGEWEGVDSALVCPDRLTAMASPALLERYGLR
CCCEEEEEECCCCCHHHHHHHHHHHEECCCCCCCCCCEEECHHHHHHHCCHHHHHHHCCC
APLAPASVLTLPLLGYTKRSWIPWLEAAGLAPVEPDTIAVFDNAAGLVAALASGLGAGLA
CCCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
RGLLAADARRDGRLVELCSIAIPTHYNLHAIWPRERHARVKPLLDAIGALVAQTLAR
HHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKRYPSIQSMQAFLQAARVGSFSSAARQLALTHSAISQQIRSLEEFIGQPLFVRTGGRV
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
SLTDAGTLLANQLSDGLEQIDRALSSVKGRASAPSLRLDVDPELMQGWLPARMPALMRAL
EECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHH
GGTTLTVLSAPRHDRDAFDRVDVALRYGYGEWEGVDSALVCPDRLTAMASPALLERYGLR
CCCEEEEEECCCCCHHHHHHHHHHHEECCCCCCCCCCEEECHHHHHHHCCHHHHHHHCCC
APLAPASVLTLPLLGYTKRSWIPWLEAAGLAPVEPDTIAVFDNAAGLVAALASGLGAGLA
CCCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
RGLLAADARRDGRLVELCSIAIPTHYNLHAIWPRERHARVKPLLDAIGALVAQTLAR
HHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2786868; 1943705 [H]