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The map label for this gene is yafC [H]

Identifier: 189353611

GI number: 189353611

Start: 1868416

End: 1869336

Strand: Direct

Name: yafC [H]

Synonym: BMULJ_04855

Alternate gene names: 189353611

Gene position: 1868416-1869336 (Clockwise)

Preceding gene: 189353609

Following gene: 189353618

Centisome position: 75.55

GC content: 68.08

Gene sequence:

>921_bases
ATGACGGACAAACTCGACGGCGTGACGACCTTCGTGCAGGTCGTGGAATCGGGCAGCTTCGCGCTGGCCGCGGAGCGGCT
CGGCATGACGCGCTCGGCGGTCGGCAAGGCGATCGCGCGGCTCGAGAAGCGGCTCGGCGCGCGGCTACTGCAGCGCACGA
CGCGCAGCCAGAGCCTGACCGACGACGGCCAGGCGTACTACGACCGCTGCGTGCGCGCGCTGGCCGAACTGGAGGCGGCG
GAAGCCGATCTCGACTGCGGCCGCAACGAGCCGCGCGGCAAACTGCGGCTCAGCGTGCCGCTCGCGTTCGGCCATCATTG
CGTGACGCCGATCGTGCTGGACCTCGCGCGCACCTATCCGCATCTGCGGATCGACGTGTCGATCACCGATCGATTCGTCG
ACCTGATCGAGGAAGGGATCGATCTCGCGGTGCGGATCGGCCCGCTCGAGGACAGCACGAGTCTCGCGGTGCGGCGGCTC
GGCACGCAATACGGTAGCCTCGGCGCGGCGCCGTCGTATCTCGCGCGCTACGGGATGCCGCAGTCGCTCGACGATCTGAA
AAACCATCGAACGGTCGCGTATTCGCGCTCGGGTGTCGTTCAGCCCTGGTACCTGCGCGCGCCGGACGGCTCCACGGTAC
ACATCGACATGCCGCACCAGCTCAGCTTCGACGACGTGCAGGCGATCGCCGCGGCCGGCGCATCGGGCTTCGGGATCGCA
TGGCTGCCGAGCTGGCTGCTCGATCAATACGTGAAGCGCGGCGAGATGGTGGTGGTGCTCGACCGCTGCTTCGTCTGCGA
AGGCGACATTCACGCGATCTGGCCGAAAACGCGCTACCTGCCGCGCAAGACGCGCTGCGTGATCGATGCGCTGGTGGAGG
CCGTGCCGCCGATGATCGAGCGCGCTCCGGCAAACGCATGA

Upstream 100 bases:

>100_bases
GCTGGACGGGAATCGGTACTATAGTGAGAATTTCGATCCCCATTGACGCGTCCACTTCCCCGAACTGCGGAACAGCAGGA
CGCAATCCGGCACACATCCC

Downstream 100 bases:

>100_bases
CGCAAACGCAGACGACACCCGACGCGCGACGCGGCACGTCGGGCGCGCGCGATCACGCGGCGCCGAGATCGGCGAGCGGA
TGCGCGAACAGCTTCCACGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTDKLDGVTTFVQVVESGSFALAAERLGMTRSAVGKAIARLEKRLGARLLQRTTRSQSLTDDGQAYYDRCVRALAELEAA
EADLDCGRNEPRGKLRLSVPLAFGHHCVTPIVLDLARTYPHLRIDVSITDRFVDLIEEGIDLAVRIGPLEDSTSLAVRRL
GTQYGSLGAAPSYLARYGMPQSLDDLKNHRTVAYSRSGVVQPWYLRAPDGSTVHIDMPHQLSFDDVQAIAAAGASGFGIA
WLPSWLLDQYVKRGEMVVVLDRCFVCEGDIHAIWPKTRYLPRKTRCVIDALVEAVPPMIERAPANA

Sequences:

>Translated_306_residues
MTDKLDGVTTFVQVVESGSFALAAERLGMTRSAVGKAIARLEKRLGARLLQRTTRSQSLTDDGQAYYDRCVRALAELEAA
EADLDCGRNEPRGKLRLSVPLAFGHHCVTPIVLDLARTYPHLRIDVSITDRFVDLIEEGIDLAVRIGPLEDSTSLAVRRL
GTQYGSLGAAPSYLARYGMPQSLDDLKNHRTVAYSRSGVVQPWYLRAPDGSTVHIDMPHQLSFDDVQAIAAAGASGFGIA
WLPSWLLDQYVKRGEMVVVLDRCFVCEGDIHAIWPKTRYLPRKTRCVIDALVEAVPPMIERAPANA
>Mature_305_residues
TDKLDGVTTFVQVVESGSFALAAERLGMTRSAVGKAIARLEKRLGARLLQRTTRSQSLTDDGQAYYDRCVRALAELEAAE
ADLDCGRNEPRGKLRLSVPLAFGHHCVTPIVLDLARTYPHLRIDVSITDRFVDLIEEGIDLAVRIGPLEDSTSLAVRRLG
TQYGSLGAAPSYLARYGMPQSLDDLKNHRTVAYSRSGVVQPWYLRAPDGSTVHIDMPHQLSFDDVQAIAAAGASGFGIAW
LPSWLLDQYVKRGEMVVVLDRCFVCEGDIHAIWPKTRYLPRKTRCVIDALVEAVPPMIERAPANA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=36.3636363636364, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=31.864406779661, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=28.9655172413793, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=27.2727272727273, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI87081978, Length=261, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789440, Length=284, Percent_Identity=27.8169014084507, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=28.1786941580756, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788706, Length=258, Percent_Identity=26.7441860465116, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789173, Length=269, Percent_Identity=23.7918215613383, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786448, Length=263, Percent_Identity=25.4752851711027, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI157672245, Length=134, Percent_Identity=32.089552238806, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=153, Percent_Identity=32.6797385620915, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=135, Percent_Identity=25.1851851851852, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=306, Percent_Identity=25.1633986928105, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33688; Mature: 33557

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKLDGVTTFVQVVESGSFALAAERLGMTRSAVGKAIARLEKRLGARLLQRTTRSQSLT
CCCCHHHHHHHHHHHHCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DDGQAYYDRCVRALAELEAAEADLDCGRNEPRGKLRLSVPLAFGHHCVTPIVLDLARTYP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHCHHHHHHHHHHHHHHCC
HLRIDVSITDRFVDLIEEGIDLAVRIGPLEDSTSLAVRRLGTQYGSLGAAPSYLARYGMP
CEEEEEEHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCC
QSLDDLKNHRTVAYSRSGVVQPWYLRAPDGSTVHIDMPHQLSFDDVQAIAAAGASGFGIA
CCHHHHHCCCEEEECCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHH
WLPSWLLDQYVKRGEMVVVLDRCFVCEGDIHAIWPKTRYLPRKTRCVIDALVEAVPPMIE
HHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHH
RAPANA
CCCCCC
>Mature Secondary Structure 
TDKLDGVTTFVQVVESGSFALAAERLGMTRSAVGKAIARLEKRLGARLLQRTTRSQSLT
CCCHHHHHHHHHHHHCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DDGQAYYDRCVRALAELEAAEADLDCGRNEPRGKLRLSVPLAFGHHCVTPIVLDLARTYP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHCHHHHHHHHHHHHHHCC
HLRIDVSITDRFVDLIEEGIDLAVRIGPLEDSTSLAVRRLGTQYGSLGAAPSYLARYGMP
CEEEEEEHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCC
QSLDDLKNHRTVAYSRSGVVQPWYLRAPDGSTVHIDMPHQLSFDDVQAIAAAGASGFGIA
CCHHHHHCCCEEEECCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHH
WLPSWLLDQYVKRGEMVVVLDRCFVCEGDIHAIWPKTRYLPRKTRCVIDALVEAVPPMIE
HHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHH
RAPANA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]