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The map label for this gene is fixJ [H]

Identifier: 189353590

GI number: 189353590

Start: 1843876

End: 1844493

Strand: Reverse

Name: fixJ [H]

Synonym: BMULJ_04834

Alternate gene names: 189353590

Gene position: 1844493-1843876 (Counterclockwise)

Preceding gene: 189353591

Following gene: 189353588

Centisome position: 74.58

GC content: 68.77

Gene sequence:

>618_bases
ATGCGGAACCTCTACCTGATCGACGACGACGACGTCATTCGCGATGCGCTCGACGCGCTGTTCCGTTCGCGCGGGCTGGC
CGCGCGAGCGTTCGGCGATGCGCGCGCATTCCTCGATGCGTGGCGCGAAGACGCGTTCGCGCGTGCGCCCGCGTGCCTGC
TGATCGATGTCCGGATGCCGGGGATGAGCGGTTTCGAGCTGTTCGCGCAACTGCGCGACGCGGGGCTCGGCGCGCATCAT
GCGGTGGTGTTCCTGACCGGGCACGGCGACATTCCGATGGCGGTCGATGCGTTGCGGGCCGGCGCCTACGATTTCCTCGA
AAAGCCGTTCTCCGACAACCGTCTGGTCGACCGCGTGGTCGCCGGGCTCGATCATGCGTACGCGCATGCGGCGCCGCCCG
AAGCGACGCCCGACGAGCGGTTCGCGAATCTGACGCCGCGCGAGCGCGCGATCGCCGAACGAATCGTCGCCGGCTGCACG
AACCGCGAAATCGCCGACGTGCTGCGCATCAGCGTCCGGACCGTCGAGGTGCACCGCGCGCGGGTATTCGAGAAGCTCGA
CGTGAAGAATGCGATCGGGCTCGCACGGTTGTGCACGCGCGCGGCGCGCCCTGCATAG

Upstream 100 bases:

>100_bases
TCTGCCGCTCGGCGCTCGAGACGATGCATTCGCGTTTGCGATACGCGCCGTCCAGCGACGGCGCGTGTTTCTACTTCGAT
TTGCGGATTACCGAAGAGTC

Downstream 100 bases:

>100_bases
CGAGCGGCCGGCGAGAACGGGCGCCGGCCGGCTAGCCGTTCGCGCCGCACCGTCCGCACGTCAGTAGGTGCGGCCGAGCT
GCAGATAGATGTTGCGCCGG

Product: LuxR family two-component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRNLYLIDDDDVIRDALDALFRSRGLAARAFGDARAFLDAWREDAFARAPACLLIDVRMPGMSGFELFAQLRDAGLGAHH
AVVFLTGHGDIPMAVDALRAGAYDFLEKPFSDNRLVDRVVAGLDHAYAHAAPPEATPDERFANLTPRERAIAERIVAGCT
NREIADVLRISVRTVEVHRARVFEKLDVKNAIGLARLCTRAARPA

Sequences:

>Translated_205_residues
MRNLYLIDDDDVIRDALDALFRSRGLAARAFGDARAFLDAWREDAFARAPACLLIDVRMPGMSGFELFAQLRDAGLGAHH
AVVFLTGHGDIPMAVDALRAGAYDFLEKPFSDNRLVDRVVAGLDHAYAHAAPPEATPDERFANLTPRERAIAERIVAGCT
NREIADVLRISVRTVEVHRARVFEKLDVKNAIGLARLCTRAARPA
>Mature_205_residues
MRNLYLIDDDDVIRDALDALFRSRGLAARAFGDARAFLDAWREDAFARAPACLLIDVRMPGMSGFELFAQLRDAGLGAHH
AVVFLTGHGDIPMAVDALRAGAYDFLEKPFSDNRLVDRVVAGLDHAYAHAAPPEATPDERFANLTPRERAIAERIVAGCT
NREIADVLRISVRTVEVHRARVFEKLDVKNAIGLARLCTRAARPA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=208, Percent_Identity=33.6538461538462, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788222, Length=214, Percent_Identity=28.5046728971963, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22576; Mature: 22576

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLYLIDDDDVIRDALDALFRSRGLAARAFGDARAFLDAWREDAFARAPACLLIDVRMP
CCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
GMSGFELFAQLRDAGLGAHHAVVFLTGHGDIPMAVDALRAGAYDFLEKPFSDNRLVDRVV
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCHHHHHHCCCCCCHHHHHHH
AGLDHAYAHAAPPEATPDERFANLTPRERAIAERIVAGCTNREIADVLRISVRTVEVHRA
HHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RVFEKLDVKNAIGLARLCTRAARPA
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRNLYLIDDDDVIRDALDALFRSRGLAARAFGDARAFLDAWREDAFARAPACLLIDVRMP
CCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
GMSGFELFAQLRDAGLGAHHAVVFLTGHGDIPMAVDALRAGAYDFLEKPFSDNRLVDRVV
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCHHHHHHCCCCCCHHHHHHH
AGLDHAYAHAAPPEATPDERFANLTPRERAIAERIVAGCTNREIADVLRISVRTVEVHRA
HHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RVFEKLDVKNAIGLARLCTRAARPA
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]