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The map label for this gene is asnB [H]

Identifier: 189353521

GI number: 189353521

Start: 1756907

End: 1758874

Strand: Reverse

Name: asnB [H]

Synonym: BMULJ_04765

Alternate gene names: 189353521

Gene position: 1758874-1756907 (Counterclockwise)

Preceding gene: 189353522

Following gene: 189353513

Centisome position: 71.12

GC content: 70.12

Gene sequence:

>1968_bases
ATGTGCGGAATCGACGGCTTTCTGAATAGCGTCGCCTTCGATGAGGAGACAGCGCGCGGCACGCTCGCGCGGATGACGGC
GAGCCTCGCGCATCGCGGACCGGACGCACAGGGCTTGTGGGTCGATCCGGCGGCCGGTATCGCGCTCGGTCACCGGCGGC
TCGCGATCGTCGACCTGTCGGTGCACGGCCGGCAGCCGATGGCGTCGGCGTGCGGCCGCTACGTGATGGTCTTCAACGGC
GAGATCTACAACCACCGCGAACTGCGCGCGCAGCTCGAGCGCGTCGGACGCGCGCCCGCGTGGCGCGGTCATTCCGACAC
CGAAGTGCTGCTCGCCGCGTTCGCCGCGTGGGGCGTCGACGCGACGTTGCGGCGCGCGACCGGGATGTTCGCGCTCGCGC
TGTGGAACCGCGAGTCGCGCGTGCTGACGCTCGCGCGCGACCGCATCGGCGAGAAACCGCTGTATTACGGGCGGATCGGC
GATGCGCTCGTATTCGCGTCGGAGCTGAAGGCGCTGCGCGCCTATCCGGGGTTCGACGGCGAGATCGATCGCGACGCGCT
GTGCCTGTATCTGCGGCATTCCGCGGTGCCCGCGCCGCATACGATCTATCGCGGCATCCGCAAGTTGCCGCCCGGCAGCT
GGATCCAGTTCGAGCATGCGCAGGACACGCCGCGAATCCGCACGTACTGGGCGCTCGAGCACGCCATCGATGCCGGCCAT
CACGAGCCGTTCGCAGGCACCGCGGCCGAGGCCGTCGACCGGCTCGACACGGTGCTGCGGCAGGCCGTCGCGCGGCAGAT
GGAAGCCGACGTGCCGCTCGGCGCGTTCCTGTCGGGCGGCGTCGATTCGTCGACGATCGTCGCGCTGATGCAGGCGCAGT
CCGCCGCGCCCGTCGACACGTTCACGATCGGTTTTCACGAAGCGGGCTACGACGAGGCCGGCTATGCGAAGGCCGTCGCG
CGCCATCTCGGCACGCGCCATACCGAGCTGTACGTGACGGCCGATCATGCGCTCGACGTCGTGCCGAAGCTGCCGGCCAT
CTACGACGAGCCGTTCTCCGACGCGTCGCAGATTCCGACCTTCCTCGTCGCGGAACTGACGCGCCGGCACGTGAAGGTCA
GCCTGTCCGGCGACGGCGGCGACGAGCTGTTCGGCGGCTATACGCGCTACTTCCTGACGCCGCGCCTCTGGCGCAAGCTG
CATCGCGTGCCGGCGGCCGTGCGTACGCGGATCGCCGGCGCGCTGCACGCGCTGCGACCCGATCATGCGGACCAGCTCGC
GGCTGTCGCGCAAGGCGCGTGGGGCGGCGGCGAACTGCGCGAATCGGCGACGCGCATCGGCGACCGGCTGCACAAGCTCG
GCCACGTGATGACGGCCGACAGCCGGATCGGGCTGTACCGGCTGCTGATGTCGTCGGTCCATCATCCGGAGCGCATCGCG
CTCGCCGGGCACGAGCCGCCGACGCCGCTCGATACGGTATCCGCATGGCCCGCGCATCTGAGCTTTGCCGAGCAGGCGAT
GGCGATCGACACGCTCACCTATCTGCCGACCGACATCCTGACGAAGGTCGACCGCGCGGCGATGGCGGTCAGCCTCGAAA
CGCGGATGCCGTTCCTCGATCATCACGTCGTCGAATTCGCGTGGCGGCTGCCCGCATCGCTGCGCTTGCCCGAAGGGCAG
TCGAAGGCGCTGCTGCGCCGGCTGCTCGACCGGTACGTGCCGACCGAGCTGATCGACCGGCCGAAGCAGGGCTTCTGCGC
GCCGATCGATCACTGGCTGCGCGGCGCGCTGCGCGACTGGGCCGACGCGTTGCTGCAGCCGTCGCGGCTGCGCGACGAGG
GCTTCTTCGACGCGGCCGCGGTCGAGCGGCTGTGGCGCCAGCATCAGACGGGGCGCATGAACTGGCAGCATCAGCTGTGG
ACCGTGCTGATGTTCCAGGCGTGGCTCGACGCGCAGCGCGCCGCCTGA

Upstream 100 bases:

>100_bases
TGAATTATTTCTTCGTAAGATGGCGCGACCGTCATGCCGAACGGCGGGCCGCAGGGTCGCGTCGCGCAGGGCGGGTAGTC
AACAGGGGCGGACAGGATAC

Downstream 100 bases:

>100_bases
CGAGGCGGTGCGCTGCGGGCGGCGCTGAACCGGCGCTGAACCGACGCTCAGCCGACGTTGATCGGCGTGCCGATCGACAG
GTGGCTCGCGTTGGCCGCGG

Product: glutamine-hydrolysing asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MCGIDGFLNSVAFDEETARGTLARMTASLAHRGPDAQGLWVDPAAGIALGHRRLAIVDLSVHGRQPMASACGRYVMVFNG
EIYNHRELRAQLERVGRAPAWRGHSDTEVLLAAFAAWGVDATLRRATGMFALALWNRESRVLTLARDRIGEKPLYYGRIG
DALVFASELKALRAYPGFDGEIDRDALCLYLRHSAVPAPHTIYRGIRKLPPGSWIQFEHAQDTPRIRTYWALEHAIDAGH
HEPFAGTAAEAVDRLDTVLRQAVARQMEADVPLGAFLSGGVDSSTIVALMQAQSAAPVDTFTIGFHEAGYDEAGYAKAVA
RHLGTRHTELYVTADHALDVVPKLPAIYDEPFSDASQIPTFLVAELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWRKL
HRVPAAVRTRIAGALHALRPDHADQLAAVAQGAWGGGELRESATRIGDRLHKLGHVMTADSRIGLYRLLMSSVHHPERIA
LAGHEPPTPLDTVSAWPAHLSFAEQAMAIDTLTYLPTDILTKVDRAAMAVSLETRMPFLDHHVVEFAWRLPASLRLPEGQ
SKALLRRLLDRYVPTELIDRPKQGFCAPIDHWLRGALRDWADALLQPSRLRDEGFFDAAAVERLWRQHQTGRMNWQHQLW
TVLMFQAWLDAQRAA

Sequences:

>Translated_655_residues
MCGIDGFLNSVAFDEETARGTLARMTASLAHRGPDAQGLWVDPAAGIALGHRRLAIVDLSVHGRQPMASACGRYVMVFNG
EIYNHRELRAQLERVGRAPAWRGHSDTEVLLAAFAAWGVDATLRRATGMFALALWNRESRVLTLARDRIGEKPLYYGRIG
DALVFASELKALRAYPGFDGEIDRDALCLYLRHSAVPAPHTIYRGIRKLPPGSWIQFEHAQDTPRIRTYWALEHAIDAGH
HEPFAGTAAEAVDRLDTVLRQAVARQMEADVPLGAFLSGGVDSSTIVALMQAQSAAPVDTFTIGFHEAGYDEAGYAKAVA
RHLGTRHTELYVTADHALDVVPKLPAIYDEPFSDASQIPTFLVAELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWRKL
HRVPAAVRTRIAGALHALRPDHADQLAAVAQGAWGGGELRESATRIGDRLHKLGHVMTADSRIGLYRLLMSSVHHPERIA
LAGHEPPTPLDTVSAWPAHLSFAEQAMAIDTLTYLPTDILTKVDRAAMAVSLETRMPFLDHHVVEFAWRLPASLRLPEGQ
SKALLRRLLDRYVPTELIDRPKQGFCAPIDHWLRGALRDWADALLQPSRLRDEGFFDAAAVERLWRQHQTGRMNWQHQLW
TVLMFQAWLDAQRAA
>Mature_655_residues
MCGIDGFLNSVAFDEETARGTLARMTASLAHRGPDAQGLWVDPAAGIALGHRRLAIVDLSVHGRQPMASACGRYVMVFNG
EIYNHRELRAQLERVGRAPAWRGHSDTEVLLAAFAAWGVDATLRRATGMFALALWNRESRVLTLARDRIGEKPLYYGRIG
DALVFASELKALRAYPGFDGEIDRDALCLYLRHSAVPAPHTIYRGIRKLPPGSWIQFEHAQDTPRIRTYWALEHAIDAGH
HEPFAGTAAEAVDRLDTVLRQAVARQMEADVPLGAFLSGGVDSSTIVALMQAQSAAPVDTFTIGFHEAGYDEAGYAKAVA
RHLGTRHTELYVTADHALDVVPKLPAIYDEPFSDASQIPTFLVAELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWRKL
HRVPAAVRTRIAGALHALRPDHADQLAAVAQGAWGGGELRESATRIGDRLHKLGHVMTADSRIGLYRLLMSSVHHPERIA
LAGHEPPTPLDTVSAWPAHLSFAEQAMAIDTLTYLPTDILTKVDRAAMAVSLETRMPFLDHHVVEFAWRLPASLRLPEGQ
SKALLRRLLDRYVPTELIDRPKQGFCAPIDHWLRGALRDWADALLQPSRLRDEGFFDAAAVERLWRQHQTGRMNWQHQLW
TVLMFQAWLDAQRAA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=424, Percent_Identity=28.0660377358491, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI168229252, Length=424, Percent_Identity=28.0660377358491, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI168229250, Length=424, Percent_Identity=28.0660377358491, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI296010848, Length=397, Percent_Identity=27.9596977329975, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI296010852, Length=322, Percent_Identity=26.3975155279503, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI296010850, Length=322, Percent_Identity=26.3975155279503, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786889, Length=458, Percent_Identity=31.4410480349345, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25147557, Length=413, Percent_Identity=29.0556900726392, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71993933, Length=410, Percent_Identity=29.0243902439024, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25147560, Length=351, Percent_Identity=27.9202279202279, Blast_Score=106, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321563, Length=431, Percent_Identity=29.0023201856148, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6325403, Length=435, Percent_Identity=28.0459770114943, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI45553209, Length=382, Percent_Identity=31.151832460733, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72751; Mature: 72751

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIDGFLNSVAFDEETARGTLARMTASLAHRGPDAQGLWVDPAAGIALGHRRLAIVDLS
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHCCCEEEEEEEE
VHGRQPMASACGRYVMVFNGEIYNHRELRAQLERVGRAPAWRGHSDTEVLLAAFAAWGVD
CCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
ATLRRATGMFALALWNRESRVLTLARDRIGEKPLYYGRIGDALVFASELKALRAYPGFDG
HHHHHHCCEEEEEEECCCCCEEEEEHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCC
EIDRDALCLYLRHSAVPAPHTIYRGIRKLPPGSWIQFEHAQDTPRIRTYWALEHAIDAGH
CCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCC
HEPFAGTAAEAVDRLDTVLRQAVARQMEADVPLGAFLSGGVDSSTIVALMQAQSAAPVDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCE
FTIGFHEAGYDEAGYAKAVARHLGTRHTELYVTADHALDVVPKLPAIYDEPFSDASQIPT
EEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCHHHHHH
FLVAELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWRKLHRVPAAVRTRIAGALHALRP
HHHHHHHHCEEEEEECCCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DHADQLAAVAQGAWGGGELRESATRIGDRLHKLGHVMTADSRIGLYRLLMSSVHHPERIA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEE
LAGHEPPTPLDTVSAWPAHLSFAEQAMAIDTLTYLPTDILTKVDRAAMAVSLETRMPFLD
ECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEHHHCCCHHH
HHVVEFAWRLPASLRLPEGQSKALLRRLLDRYVPTELIDRPKQGFCAPIDHWLRGALRDW
HHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHH
ADALLQPSRLRDEGFFDAAAVERLWRQHQTGRMNWQHQLWTVLMFQAWLDAQRAA
HHHHCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIDGFLNSVAFDEETARGTLARMTASLAHRGPDAQGLWVDPAAGIALGHRRLAIVDLS
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHCCCEEEEEEEE
VHGRQPMASACGRYVMVFNGEIYNHRELRAQLERVGRAPAWRGHSDTEVLLAAFAAWGVD
CCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
ATLRRATGMFALALWNRESRVLTLARDRIGEKPLYYGRIGDALVFASELKALRAYPGFDG
HHHHHHCCEEEEEEECCCCCEEEEEHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCC
EIDRDALCLYLRHSAVPAPHTIYRGIRKLPPGSWIQFEHAQDTPRIRTYWALEHAIDAGH
CCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCC
HEPFAGTAAEAVDRLDTVLRQAVARQMEADVPLGAFLSGGVDSSTIVALMQAQSAAPVDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCE
FTIGFHEAGYDEAGYAKAVARHLGTRHTELYVTADHALDVVPKLPAIYDEPFSDASQIPT
EEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCHHHHHH
FLVAELTRRHVKVSLSGDGGDELFGGYTRYFLTPRLWRKLHRVPAAVRTRIAGALHALRP
HHHHHHHHCEEEEEECCCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DHADQLAAVAQGAWGGGELRESATRIGDRLHKLGHVMTADSRIGLYRLLMSSVHHPERIA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEE
LAGHEPPTPLDTVSAWPAHLSFAEQAMAIDTLTYLPTDILTKVDRAAMAVSLETRMPFLD
ECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEHHHCCCHHH
HHVVEFAWRLPASLRLPEGQSKALLRRLLDRYVPTELIDRPKQGFCAPIDHWLRGALRDW
HHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHH
ADALLQPSRLRDEGFFDAAAVERLWRQHQTGRMNWQHQLWTVLMFQAWLDAQRAA
HHHHCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]