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The map label for this gene is gutB [H]

Identifier: 189353488

GI number: 189353488

Start: 1705744

End: 1706832

Strand: Reverse

Name: gutB [H]

Synonym: BMULJ_04732

Alternate gene names: 189353488

Gene position: 1706832-1705744 (Counterclockwise)

Preceding gene: 189353489

Following gene: 189353487

Centisome position: 69.01

GC content: 66.85

Gene sequence:

>1089_bases
ATGACGACACCCGAAGCCCAGCCGCGGATGACCGCGGTCGTCTGCCACGGCCCCGAGGACTATCGCGTCGAACAGGTCGC
GAAGCCGCGCGCCGGCGCGAACGAACTCGTGATCCGGATCGCCGCATGCGGCATCTGCGCGAGCGACTGCAAATGCTATA
CGGGCGCGAAGATGTTCTGGGGCGGCCCGAACCCATGGGTGAAGGCGCCCGTGATTCCCGGCCACGAATTCTTCGGCTAC
GTCGAAGCGCTCGGCGAGGGCGCGGCCGAGCACTTCGGCGTCGCGCTCGGCGACCGCGTGATCGCCGAGCAGATCGTGCC
GTGCGGCAAGTGCCGCTACTGCAAGTCGGGCCAGTACTGGATGTGCGAGGTGCACAACATCTTCGGCTTCCAGCGCGAGG
TGGCCGACGGCGGAATGGCCGAGTACATGCGCATTCCGCCGACCGCGATCGTGCACCGGATTCCGCTCGGCGTGTCGCTC
GAGGACGCGGCGATCATCGAGCCGCTGTCGTGCGCGATCCATACCGTGAATCGCGGCGACATCCAGCTCGACGACGTCGT
CGTGATCGCCGGCGCGGGCCCGCTCGGGCTGATGATGACGCAGGTCGCGCATCTGAAGACGCCGAAGAAGCTCGTCGTGA
TCGACCTGATCGACGAGCGGCTCGAGCTTGCGCGGCAGTACGGCGCCGACGTGACGATCAACCCGCAGCGCGACGATGCA
CGCGAGATCGTGCGAGCGCTGACCGACGGCTACGGCTGCGACGTCTATATCGAAACGACCGGCGCGCCGGTCGGCGTGAA
TCAGGGGCTCGAGCTGATCCGCAAGCTCGGGCGCTTCGTCGAATTCAGCGTGTTCGGCGAGGATGCGACGGTCGACTGGT
CGATCATCGGCGATCGCAAGGAGCTCGACGTGCGCGGCGCGCATCTCGGCCCGTACTGCTATCCGATCGCGATCGATCTG
CTCGCGCGCGGGCTCGTCACGTCGAAAGGCATCGTCACGCACGGCTTCGCGCTCGAGGACTGGGACGAGGCGATCCGCGT
CGCGAAATCGCCGGAATCGATCAAGGTGCTGTTGAAGCCCGCGCGCTGA

Upstream 100 bases:

>100_bases
ATCATCCTGCTCGCGATCTACATCAGCCGCGAACGTTCGCGGTGACGCGGCCGCCTCGCCGGCCCTCCAACCTTTCTCCT
TGACCGAAACGAGACAACTC

Downstream 100 bases:

>100_bases
GCAATCGCGTGCGCATGACGGGAGGCATCATGGAATACGTGATTGGCGTCGACATCGGCACGCAGAGCACCAAGGCGCTG
CTCGTCGACCGGCACGGCAC

Product: L-iditol 2-dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTTPEAQPRMTAVVCHGPEDYRVEQVAKPRAGANELVIRIAACGICASDCKCYTGAKMFWGGPNPWVKAPVIPGHEFFGY
VEALGEGAAEHFGVALGDRVIAEQIVPCGKCRYCKSGQYWMCEVHNIFGFQREVADGGMAEYMRIPPTAIVHRIPLGVSL
EDAAIIEPLSCAIHTVNRGDIQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLIDERLELARQYGADVTINPQRDDA
REIVRALTDGYGCDVYIETTGAPVGVNQGLELIRKLGRFVEFSVFGEDATVDWSIIGDRKELDVRGAHLGPYCYPIAIDL
LARGLVTSKGIVTHGFALEDWDEAIRVAKSPESIKVLLKPAR

Sequences:

>Translated_362_residues
MTTPEAQPRMTAVVCHGPEDYRVEQVAKPRAGANELVIRIAACGICASDCKCYTGAKMFWGGPNPWVKAPVIPGHEFFGY
VEALGEGAAEHFGVALGDRVIAEQIVPCGKCRYCKSGQYWMCEVHNIFGFQREVADGGMAEYMRIPPTAIVHRIPLGVSL
EDAAIIEPLSCAIHTVNRGDIQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLIDERLELARQYGADVTINPQRDDA
REIVRALTDGYGCDVYIETTGAPVGVNQGLELIRKLGRFVEFSVFGEDATVDWSIIGDRKELDVRGAHLGPYCYPIAIDL
LARGLVTSKGIVTHGFALEDWDEAIRVAKSPESIKVLLKPAR
>Mature_361_residues
TTPEAQPRMTAVVCHGPEDYRVEQVAKPRAGANELVIRIAACGICASDCKCYTGAKMFWGGPNPWVKAPVIPGHEFFGYV
EALGEGAAEHFGVALGDRVIAEQIVPCGKCRYCKSGQYWMCEVHNIFGFQREVADGGMAEYMRIPPTAIVHRIPLGVSLE
DAAIIEPLSCAIHTVNRGDIQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLIDERLELARQYGADVTINPQRDDAR
EIVRALTDGYGCDVYIETTGAPVGVNQGLELIRKLGRFVEFSVFGEDATVDWSIIGDRKELDVRGAHLGPYCYPIAIDLL
ARGLVTSKGIVTHGFALEDWDEAIRVAKSPESIKVLLKPAR

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=356, Percent_Identity=33.1460674157303, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI4501929, Length=382, Percent_Identity=24.3455497382199, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI71565154, Length=366, Percent_Identity=24.3169398907104, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4501933, Length=381, Percent_Identity=23.0971128608924, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI34577061, Length=266, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787863, Length=353, Percent_Identity=31.1614730878187, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790045, Length=344, Percent_Identity=31.1046511627907, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788073, Length=339, Percent_Identity=26.2536873156342, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1788407, Length=337, Percent_Identity=28.7833827893175, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1788075, Length=363, Percent_Identity=28.3746556473829, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI226510992, Length=337, Percent_Identity=28.7833827893175, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1790718, Length=318, Percent_Identity=29.2452830188679, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87082125, Length=363, Percent_Identity=29.2011019283747, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1786825, Length=276, Percent_Identity=30.0724637681159, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081918, Length=286, Percent_Identity=29.020979020979, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562876, Length=359, Percent_Identity=33.4261838440111, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17562878, Length=357, Percent_Identity=31.6526610644258, Blast_Score=160, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI25146526, Length=375, Percent_Identity=26.4, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=26.1333333333333, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565904, Length=252, Percent_Identity=27.3809523809524, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562584, Length=324, Percent_Identity=26.2345679012346, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323099, Length=343, Percent_Identity=30.9037900874636, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322619, Length=364, Percent_Identity=30.2197802197802, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319955, Length=364, Percent_Identity=30.2197802197802, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6319258, Length=275, Percent_Identity=26.9090909090909, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320033, Length=298, Percent_Identity=27.8523489932886, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319257, Length=368, Percent_Identity=27.1739130434783, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=338, Percent_Identity=28.1065088757396, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319621, Length=256, Percent_Identity=28.515625, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323961, Length=337, Percent_Identity=26.4094955489614, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323729, Length=335, Percent_Identity=25.0746268656716, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17137530, Length=350, Percent_Identity=30, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI17737897, Length=360, Percent_Identity=30.2777777777778, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17737895, Length=367, Percent_Identity=26.158038147139, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI221457811, Length=344, Percent_Identity=24.4186046511628, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI45551930, Length=344, Percent_Identity=24.4186046511628, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550770, Length=344, Percent_Identity=24.4186046511628, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 39426; Mature: 39295

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPEAQPRMTAVVCHGPEDYRVEQVAKPRAGANELVIRIAACGICASDCKCYTGAKMFW
CCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCHHHEEEEEECCCCCCCCCEEECCCEEEE
GGPNPWVKAPVIPGHEFFGYVEALGEGAAEHFGVALGDRVIAEQIVPCGKCRYCKSGQYW
CCCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
MCEVHNIFGFQREVADGGMAEYMRIPPTAIVHRIPLGVSLEDAAIIEPLSCAIHTVNRGD
EEEEHHHHCHHHHHCCCCHHHHHCCCHHHHHHHCCCCCCCCCHHHHCHHHHEEEECCCCC
IQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLIDERLELARQYGADVTINPQRDDA
EEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCEEECCCCCHH
REIVRALTDGYGCDVYIETTGAPVGVNQGLELIRKLGRFVEFSVFGEDATVDWSIIGDRK
HHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCC
ELDVRGAHLGPYCYPIAIDLLARGLVTSKGIVTHGFALEDWDEAIRVAKSPESIKVLLKP
CCEECCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHHCHHHHHHHCCCCCEEEEEEEC
AR
CC
>Mature Secondary Structure 
TTPEAQPRMTAVVCHGPEDYRVEQVAKPRAGANELVIRIAACGICASDCKCYTGAKMFW
CCCCCCCCEEEEEEECCCCCCHHHHHCCCCCHHHEEEEEECCCCCCCCCEEECCCEEEE
GGPNPWVKAPVIPGHEFFGYVEALGEGAAEHFGVALGDRVIAEQIVPCGKCRYCKSGQYW
CCCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
MCEVHNIFGFQREVADGGMAEYMRIPPTAIVHRIPLGVSLEDAAIIEPLSCAIHTVNRGD
EEEEHHHHCHHHHHCCCCHHHHHCCCHHHHHHHCCCCCCCCCHHHHCHHHHEEEECCCCC
IQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLIDERLELARQYGADVTINPQRDDA
EEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCEEECCCCCHH
REIVRALTDGYGCDVYIETTGAPVGVNQGLELIRKLGRFVEFSVFGEDATVDWSIIGDRK
HHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCC
ELDVRGAHLGPYCYPIAIDLLARGLVTSKGIVTHGFALEDWDEAIRVAKSPESIKVLLKP
CCEECCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHHCHHHHHHHCCCCCEEEEEEEC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]