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The map label for this gene is pyrB [H]

Identifier: 189353476

GI number: 189353476

Start: 1694898

End: 1696193

Strand: Direct

Name: pyrB [H]

Synonym: BMULJ_04720

Alternate gene names: 189353476

Gene position: 1694898-1696193 (Clockwise)

Preceding gene: 189353474

Following gene: 189353479

Centisome position: 68.53

GC content: 65.51

Gene sequence:

>1296_bases
ATGCCCGTTCCTCAGCAAGCCTTCCTCCGCGACGCGATGCGCCGCCTCAACATGACGCGCGAAGCGTTCGCGAACCGCAT
CGGCGTGAGCCGCCGTGCGCTAGATACCTGGCTGCTACCCGACGATTCGCAGGAATCGCGCGGCATGCCGGAGATCGTCG
AGCGGTTCGTGTCGGAGATCGTCGAGCGCGCGGCGCCGGAAGGCGAAATCTATACGCAACGCGTAGATAACCACGGCCTC
GCGAAGCAGTTCCTGTTCGAAGGCAAGCCGCAACTGCTGTCGGTCGACCAGTTCTCGCGCGAGTCGGTCGAGGCGCTGTT
CCGCGTCGCCGACGTGATGCAGCCGATCGCGCGCCGCCACAAGATTTCGCGCGTGCTCGAAGGCGCGGTGCTCGGCAACC
TGTTCTTCGAAGCCAGCACGCGCACGCGCGTGTCGTTCGGCGCGGCGTTCTGCCGGCTCGGCGGCTCCGTCTGCGACACG
ACCGGGTTCACGTTCTCGTCGATGGCGAAAGGCGAGTCGATCTACGATACGAGCCGCGTGATGGCCGGCTACGTCGACGC
GCTCGTGATCCGCCATCCGGAGAAAGGCTCGGTCGCCGAATTCGCGCGCGCGACCAATCTGCCGGTGATCAACGGCGGCG
ACGGTCCGGGCGAGCATCCGAGCCAGGCGCTGCTCGATCTCTATACGATCCAGCGCGAATTCTCGCGGCTCGGCAAGATC
GTCGACGGCGCGCACATCGCGCTCGTCGGCGACCTCAAATACGGCCGGACCGTGCATTCGCTCGTCAAGCTGCTCGCGCT
CTACCGCGGGCTCAAATTCACGCTGGTGTCGCCGCCGACGCTCGAAATGCCCGCCTACATCATCGACCAGATCGCGACGA
ACGGGCACGTGATCGAGCAGACGAACGACCTCGCGGCGGGGCTGCGCGGCGCCGATGTCGTCTATGCGACGCGAATCCAG
AAGGAGCGCTTCACCGACGAGTCGTTCGAAGGCTATACGCCCGATTTCCAGATCAATCAGGCGCTCGTCGACACGGTCTG
CAAACCCGACACGCTGATCATGCATCCGCTGCCGCGCGACAGCCGGCCCGGCGCCAACGATCTGTCGACCGACCTGAACC
GCGATCCGCGTCTCGCGATCTTCCGGCAGACCGACAACGGCATTCCGGTGCGCATGGCGATCTTTGCCGTGCTGCTCGGC
GTCGAAAACCTCGTGCAGCATTCGATGCGCGACGCGACGTGGCGACCGCCGGCATACCTGGGGCCGGAGGATGCCGTGTT
TCACGGGATCGACTGA

Upstream 100 bases:

>100_bases
GTATCGCGCGCGCGACACGGTCCGGCGCGAGATTTTCCTTGACTTCGGCCCATGCGCGACTAATATACGCACCGCGTATA
TTTTTCATCAGGTGCCGCCG

Downstream 100 bases:

>100_bases
TATTCGTCGAATCGCCCGGCCGGCACGCGATTGCGTCGGGCCGGCGTGCCGCGCACCGCATCATGAACAGCGCGCCGGAC
CGCCCGGCGCGCTGTTTTCA

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEIVERAAPEGEIYTQRVDNHGL
AKQFLFEGKPQLLSVDQFSRESVEALFRVADVMQPIARRHKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDT
TGFTFSSMAKGESIYDTSRVMAGYVDALVIRHPEKGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKI
VDGAHIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIIDQIATNGHVIEQTNDLAAGLRGADVVYATRIQ
KERFTDESFEGYTPDFQINQALVDTVCKPDTLIMHPLPRDSRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLG
VENLVQHSMRDATWRPPAYLGPEDAVFHGID

Sequences:

>Translated_431_residues
MPVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEIVERAAPEGEIYTQRVDNHGL
AKQFLFEGKPQLLSVDQFSRESVEALFRVADVMQPIARRHKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDT
TGFTFSSMAKGESIYDTSRVMAGYVDALVIRHPEKGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKI
VDGAHIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIIDQIATNGHVIEQTNDLAAGLRGADVVYATRIQ
KERFTDESFEGYTPDFQINQALVDTVCKPDTLIMHPLPRDSRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLG
VENLVQHSMRDATWRPPAYLGPEDAVFHGID
>Mature_430_residues
PVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEIVERAAPEGEIYTQRVDNHGLA
KQFLFEGKPQLLSVDQFSRESVEALFRVADVMQPIARRHKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTT
GFTFSSMAKGESIYDTSRVMAGYVDALVIRHPEKGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKIV
DGAHIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIIDQIATNGHVIEQTNDLAAGLRGADVVYATRIQK
ERFTDESFEGYTPDFQINQALVDTVCKPDTLIMHPLPRDSRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLGV
ENLVQHSMRDATWRPPAYLGPEDAVFHGID

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=41.8006430868167, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI38788445, Length=319, Percent_Identity=26.9592476489028, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI2367364, Length=320, Percent_Identity=36.5625, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1790703, Length=277, Percent_Identity=27.0758122743682, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786469, Length=281, Percent_Identity=27.0462633451957, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=327, Percent_Identity=42.5076452599388, Blast_Score=231, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=36.2244897959184, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322373, Length=334, Percent_Identity=25.748502994012, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24642586, Length=341, Percent_Identity=38.7096774193548, Blast_Score=222, Evalue=5e-58,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 47858; Mature: 47727

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHH
VERAAPEGEIYTQRVDNHGLAKQFLFEGKPQLLSVDQFSRESVEALFRVADVMQPIARRH
HHHCCCCCCEEHHHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTTGFTFSSMAKGESIYDTSRV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHHCCCHHHHHHHH
MAGYVDALVIRHPEKGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKI
HHHHHHHHHEECCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VDGAHIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIIDQIATNGHVIEQ
HCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEC
TNDLAAGLRGADVVYATRIQKERFTDESFEGYTPDFQINQALVDTVCKPDTLIMHPLPRD
CCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCC
SRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLGVENLVQHSMRDATWRPPAYL
CCCCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPEDAVFHGID
CCCCCCCCCCC
>Mature Secondary Structure 
PVPQQAFLRDAMRRLNMTREAFANRIGVSRRALDTWLLPDDSQESRGMPEIVERFVSEI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHH
VERAAPEGEIYTQRVDNHGLAKQFLFEGKPQLLSVDQFSRESVEALFRVADVMQPIARRH
HHHCCCCCCEEHHHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTTGFTFSSMAKGESIYDTSRV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHHCCCHHHHHHHH
MAGYVDALVIRHPEKGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKI
HHHHHHHHHEECCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VDGAHIALVGDLKYGRTVHSLVKLLALYRGLKFTLVSPPTLEMPAYIIDQIATNGHVIEQ
HCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEC
TNDLAAGLRGADVVYATRIQKERFTDESFEGYTPDFQINQALVDTVCKPDTLIMHPLPRD
CCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCC
SRPGANDLSTDLNRDPRLAIFRQTDNGIPVRMAIFAVLLGVENLVQHSMRDATWRPPAYL
CCCCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPEDAVFHGID
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA