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The map label for this gene is nemA [H]

Identifier: 189353369

GI number: 189353369

Start: 1618443

End: 1619504

Strand: Direct

Name: nemA [H]

Synonym: BMULJ_04609

Alternate gene names: 189353369

Gene position: 1618443-1619504 (Clockwise)

Preceding gene: 189353368

Following gene: 189353371

Centisome position: 65.44

GC content: 70.24

Gene sequence:

>1062_bases
ATGCCCACGCTGTTCGACCCCGTTACCCTCGGCGATCTGAAGCTGCCGAACCGCATCGTGATGGCACCGCTGACGCGCAG
CCGCGCCGGCGACACGCGCATCCCGAACGACCTGATGGCGCGCTACTACGCCGAACGCGCGTCGGCGGGCCTGATCATCA
GCGAGGCGACGTCGGTCACGCCGCAAGGCGTCGGCTATGCAGGCACGCCCGGCATCTGGTCGGACGAGCAGGTCGAAGGC
TGGAAGCGCGTGACGCAGGCCGTCCATCGCGCGGGTGGCAGCATCGTGCTGCAGCTCTGGCACGTCGGCCGGATCTCGGA
TCCGATGTTCCTGAACGGCGATCTGCCGGTCGCGCCGAGCGCGATCGCGGCGGGCGGCCACGTGAGCCTCGTGCGTCCGC
AGCGGCCGTTCGTGACGCCGCGCGCGCTCGAACTCGACGAGATCCCCGGCATCATTGCGGCCTATCGCAAGGGCGCCGAA
AACGCGAAGGCGGCCGGCTTCGACGGCGTCGAGATCCACGGCGCGAACGGCTATCTGCTCGACCAGTTCCTGCAGGACAG
CACGAACCGCCGCACCGACGCGTACGGCGGCCCGATCGAGAACCGTGCGCGGTTGCTGCTCGAAGTCGTCGACGCGGCGA
TCGACGTGTGGGGCGCCGGGCGTGTCGGCGTGCACCTCGCGCCGCGCGGCGATGCGCACACGATGGGCGATTCCGACCCG
GCCGCGACGTTCGGCTATGTCGCGCGCGAACTCGGCCGCCGCCGCATCGCGTTCATCTTCACGCGCGAGTCGTACAGCGG
CGACCACCTGAGCCCGCAGCTGAAAGAGGCGTTCGGCGGCCCGCTGATCGCGAACGAGCAGTTCACGTTCGACAGCGCGC
AGGCCGCACTCGCGCGCGGCGACGCCGACGCGATCGCATGGGGCAAGCTGTTCATCGCGAATCCGGACCTGCCGCGCCGC
CTCGAACTCGGCGCGCCGCTGAACCAGCCGGTGCCCGAAACCTTCTACGCGGAAGGCGAGACCGGCTACACCGACTATCC
GGCGCTCAGCGACGCCGCCTGA

Upstream 100 bases:

>100_bases
CACTGCGCCGAGCACTCTGACCATCCGGCCGAACCGGCCATGCGGGCCCGCGCCGCGCGATGCGCACGCCGGTCCCGCCC
TTCGTGAAGAGGTAATTCGT

Downstream 100 bases:

>100_bases
GCGTCGTCCTTCGCGTGTTCACGCGCGCACGTCCGCGCGCATGAACACGCGCTGCGTCTCGTCGAGGCCGCGCGCGACGT
GATGCACGCGGATCGCGCGC

Product: NADPH2 dehydrogenase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MPTLFDPVTLGDLKLPNRIVMAPLTRSRAGDTRIPNDLMARYYAERASAGLIISEATSVTPQGVGYAGTPGIWSDEQVEG
WKRVTQAVHRAGGSIVLQLWHVGRISDPMFLNGDLPVAPSAIAAGGHVSLVRPQRPFVTPRALELDEIPGIIAAYRKGAE
NAKAAGFDGVEIHGANGYLLDQFLQDSTNRRTDAYGGPIENRARLLLEVVDAAIDVWGAGRVGVHLAPRGDAHTMGDSDP
AATFGYVARELGRRRIAFIFTRESYSGDHLSPQLKEAFGGPLIANEQFTFDSAQAALARGDADAIAWGKLFIANPDLPRR
LELGAPLNQPVPETFYAEGETGYTDYPALSDAA

Sequences:

>Translated_353_residues
MPTLFDPVTLGDLKLPNRIVMAPLTRSRAGDTRIPNDLMARYYAERASAGLIISEATSVTPQGVGYAGTPGIWSDEQVEG
WKRVTQAVHRAGGSIVLQLWHVGRISDPMFLNGDLPVAPSAIAAGGHVSLVRPQRPFVTPRALELDEIPGIIAAYRKGAE
NAKAAGFDGVEIHGANGYLLDQFLQDSTNRRTDAYGGPIENRARLLLEVVDAAIDVWGAGRVGVHLAPRGDAHTMGDSDP
AATFGYVARELGRRRIAFIFTRESYSGDHLSPQLKEAFGGPLIANEQFTFDSAQAALARGDADAIAWGKLFIANPDLPRR
LELGAPLNQPVPETFYAEGETGYTDYPALSDAA
>Mature_352_residues
PTLFDPVTLGDLKLPNRIVMAPLTRSRAGDTRIPNDLMARYYAERASAGLIISEATSVTPQGVGYAGTPGIWSDEQVEGW
KRVTQAVHRAGGSIVLQLWHVGRISDPMFLNGDLPVAPSAIAAGGHVSLVRPQRPFVTPRALELDEIPGIIAAYRKGAEN
AKAAGFDGVEIHGANGYLLDQFLQDSTNRRTDAYGGPIENRARLLLEVVDAAIDVWGAGRVGVHLAPRGDAHTMGDSDPA
ATFGYVARELGRRRIAFIFTRESYSGDHLSPQLKEAFGGPLIANEQFTFDSAQAALARGDADAIAWGKLFIANPDLPRRL
ELGAPLNQPVPETFYAEGETGYTDYPALSDAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=364, Percent_Identity=47.2527472527472, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789463, Length=343, Percent_Identity=31.7784256559767, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=327, Percent_Identity=27.217125382263, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=357, Percent_Identity=26.890756302521, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=317, Percent_Identity=27.4447949526814, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=321, Percent_Identity=26.791277258567, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=315, Percent_Identity=27.3015873015873, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=377, Percent_Identity=26.790450928382, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=357, Percent_Identity=25.7703081232493, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=39.3700787401575, Blast_Score=222, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6321973, Length=380, Percent_Identity=38.4210526315789, Blast_Score=213, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37913; Mature: 37782

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLFDPVTLGDLKLPNRIVMAPLTRSRAGDTRIPNDLMARYYAERASAGLIISEATSVT
CCCCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
PQGVGYAGTPGIWSDEQVEGWKRVTQAVHRAGGSIVLQLWHVGRISDPMFLNGDLPVAPS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCCH
AIAAGGHVSLVRPQRPFVTPRALELDEIPGIIAAYRKGAENAKAAGFDGVEIHGANGYLL
HHCCCCEEEEECCCCCCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCCCEEEECCCCHHH
DQFLQDSTNRRTDAYGGPIENRARLLLEVVDAAIDVWGAGRVGVHLAPRGDAHTMGDSDP
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCH
AATFGYVARELGRRRIAFIFTRESYSGDHLSPQLKEAFGGPLIANEQFTFDSAQAALARG
HHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEECCCCCCHHHHHHHHCC
DADAIAWGKLFIANPDLPRRLELGAPLNQPVPETFYAEGETGYTDYPALSDAA
CCCEEEECEEEEECCCCCCEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTLFDPVTLGDLKLPNRIVMAPLTRSRAGDTRIPNDLMARYYAERASAGLIISEATSVT
CCCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
PQGVGYAGTPGIWSDEQVEGWKRVTQAVHRAGGSIVLQLWHVGRISDPMFLNGDLPVAPS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCCH
AIAAGGHVSLVRPQRPFVTPRALELDEIPGIIAAYRKGAENAKAAGFDGVEIHGANGYLL
HHCCCCEEEEECCCCCCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCCCEEEECCCCHHH
DQFLQDSTNRRTDAYGGPIENRARLLLEVVDAAIDVWGAGRVGVHLAPRGDAHTMGDSDP
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCH
AATFGYVARELGRRRIAFIFTRESYSGDHLSPQLKEAFGGPLIANEQFTFDSAQAALARG
HHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEECCCCCCHHHHHHHHCC
DADAIAWGKLFIANPDLPRRLELGAPLNQPVPETFYAEGETGYTDYPALSDAA
CCCEEEECEEEEECCCCCCEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]