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The map label for this gene is pdhD [H]

Identifier: 189353312

GI number: 189353312

Start: 1549974

End: 1551368

Strand: Direct

Name: pdhD [H]

Synonym: BMULJ_04552

Alternate gene names: 189353312

Gene position: 1549974-1551368 (Clockwise)

Preceding gene: 189353302

Following gene: 189353314

Centisome position: 62.67

GC content: 66.81

Gene sequence:

>1395_bases
ATGCGCACGATTTACACGAATGTCGCCGTCATCGGCGCAGGCACCGCGGGGCTCGCCGCTTATCGTGCGGCGCGCGCCGA
AGGCGCCACCGCCGTCATTATCGAAGGCGGTCCGTACGGCACGACCTGTGCCCGCGTCGGCTGCATGCCCTCAAAGTTGC
TGATCGCGGCCGCTGAGGCCGCGCATGCAGCGAGCCACGCCGCACCGTTCGGCATCGCGGTCGGCAACGTGCGCGTCGAC
GGAGAAGCGGTGATGGCGCGCGTACGACGCGAACGCGATCGTTTCGTCGGATTCGTCGTCGACGGCGTCGAAGCATTGCC
CGTTGAAGATCGCCTGGTCGGGTACGCACGCTTCGTCGATGACGGGCTGCTGCAGGTGGGCGATCACACGCAGGTGCGCG
CGGCGAGCGTCGTGATCGCTACGGGCTCCACGCCGACCATTCCGGCGGTCCTGAACGGCGTTCGCGATCGCGTGATCGTC
AACGATGACGTGTTCGCATGGACGACGCTGCCGCGCAGTGTCGCCGTGATCGGCCCCGGCGTGATCGGCCTCGAACTCGG
GCAGGCGCTTGCGCGACTCGGCGTACGTGTGAGTGTGTACGGCGCGCGCGGCAGCGTCGGTCAACTGACCGACCCGAAGC
TCGTCGAGGCCGCGCGCGCGATCTTCGGCGACGCATTTCATTTCGAGCCGAGCGGCACGATGCTGAGTGCGACGCGCATC
GACGACGGCGTCACGCTACGCTATCGCCGCGCCGACGGATCGACGATCGACGCGACCTACGAGTACGTGCTCGCGGCCAC
CGGCAGGCATCCCGACCTCGGCAACCTGGGGCTCGCGAACACGTCGATCCAACTGGGCGAACACGGCATACCGCGGTTCG
ACGCGCAGACGTTGCAGGTCCAGGGACACCCGGTCTTCATCGCGGGCGACGCGAACGGCATTCTGCCGCTGCTGCATGAA
GCGGTCGACGAAGGTCGCGCGGCCGGCCGCAACGCCGCGCGCTATCCGGACGTCGCAAAAATCGAGCGCCGTGCGCCGCT
CGCGATCGTGTTTTCCGATCCGCAGATCGCGCTCGTCGGCGCACGCCATCGCGAGTTGCGCGCAGACACGTTCGTGACGG
GCGAAGTGAGCTTCGAAGACCAGGGACGCAGTCGCGTGATGTTGAAAAATCTCGGGCGCGCGCATGTCTACGTCGACCGC
GCGACACGACGATTCGTCGGTGCAGAGATGCTGGGCCCATCCGCCGAACATATCGGGCATTTGCTCGCGTGGAGCTTGCA
GATGGAACTCACCGTCGATGCGATGCTCGCGATGCCGTTCTATCATCCGGTCGTCGAAGAGGGCGTGCGTACCGCGCTCA
TGGATGCCGCCGCCAAACTCGACGCGCCGCAGTAG

Upstream 100 bases:

>100_bases
GCTGCGCGCTGCCTACACTGAGCGCAGGCCGTAACCGACGCGTCGCGACAGCAAACGTGACGGAATCGTCGCATCCGGCC
GCTCAATCGGGAGGCAGGCA

Downstream 100 bases:

>100_bases
CGCATCGCGCGGTTCGGCCGGTCGTCTCGTCGGCATTCGACAGATCGCGCATCTCAATGACCCGCGCTCTGCCCGCCGTC
CACATGGAGGATCTCGCCGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRTIYTNVAVIGAGTAGLAAYRAARAEGATAVIIEGGPYGTTCARVGCMPSKLLIAAAEAAHAASHAAPFGIAVGNVRVD
GEAVMARVRRERDRFVGFVVDGVEALPVEDRLVGYARFVDDGLLQVGDHTQVRAASVVIATGSTPTIPAVLNGVRDRVIV
NDDVFAWTTLPRSVAVIGPGVIGLELGQALARLGVRVSVYGARGSVGQLTDPKLVEAARAIFGDAFHFEPSGTMLSATRI
DDGVTLRYRRADGSTIDATYEYVLAATGRHPDLGNLGLANTSIQLGEHGIPRFDAQTLQVQGHPVFIAGDANGILPLLHE
AVDEGRAAGRNAARYPDVAKIERRAPLAIVFSDPQIALVGARHRELRADTFVTGEVSFEDQGRSRVMLKNLGRAHVYVDR
ATRRFVGAEMLGPSAEHIGHLLAWSLQMELTVDAMLAMPFYHPVVEEGVRTALMDAAAKLDAPQ

Sequences:

>Translated_464_residues
MRTIYTNVAVIGAGTAGLAAYRAARAEGATAVIIEGGPYGTTCARVGCMPSKLLIAAAEAAHAASHAAPFGIAVGNVRVD
GEAVMARVRRERDRFVGFVVDGVEALPVEDRLVGYARFVDDGLLQVGDHTQVRAASVVIATGSTPTIPAVLNGVRDRVIV
NDDVFAWTTLPRSVAVIGPGVIGLELGQALARLGVRVSVYGARGSVGQLTDPKLVEAARAIFGDAFHFEPSGTMLSATRI
DDGVTLRYRRADGSTIDATYEYVLAATGRHPDLGNLGLANTSIQLGEHGIPRFDAQTLQVQGHPVFIAGDANGILPLLHE
AVDEGRAAGRNAARYPDVAKIERRAPLAIVFSDPQIALVGARHRELRADTFVTGEVSFEDQGRSRVMLKNLGRAHVYVDR
ATRRFVGAEMLGPSAEHIGHLLAWSLQMELTVDAMLAMPFYHPVVEEGVRTALMDAAAKLDAPQ
>Mature_464_residues
MRTIYTNVAVIGAGTAGLAAYRAARAEGATAVIIEGGPYGTTCARVGCMPSKLLIAAAEAAHAASHAAPFGIAVGNVRVD
GEAVMARVRRERDRFVGFVVDGVEALPVEDRLVGYARFVDDGLLQVGDHTQVRAASVVIATGSTPTIPAVLNGVRDRVIV
NDDVFAWTTLPRSVAVIGPGVIGLELGQALARLGVRVSVYGARGSVGQLTDPKLVEAARAIFGDAFHFEPSGTMLSATRI
DDGVTLRYRRADGSTIDATYEYVLAATGRHPDLGNLGLANTSIQLGEHGIPRFDAQTLQVQGHPVFIAGDANGILPLLHE
AVDEGRAAGRNAARYPDVAKIERRAPLAIVFSDPQIALVGARHRELRADTFVTGEVSFEDQGRSRVMLKNLGRAHVYVDR
ATRRFVGAEMLGPSAEHIGHLLAWSLQMELTVDAMLAMPFYHPVVEEGVRTALMDAAAKLDAPQ

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=477, Percent_Identity=26.8343815513627, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1786307, Length=461, Percent_Identity=25.1626898047722, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=23.7894736842105, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789915, Length=351, Percent_Identity=28.2051282051282, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32565766, Length=467, Percent_Identity=25.9100642398287, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=352, Percent_Identity=25.5681818181818, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983419, Length=352, Percent_Identity=25.5681818181818, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321091, Length=492, Percent_Identity=25.2032520325203, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325166, Length=392, Percent_Identity=27.0408163265306, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325240, Length=347, Percent_Identity=23.0547550432277, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=26.1146496815287, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24640549, Length=489, Percent_Identity=26.3803680981595, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24640553, Length=489, Percent_Identity=26.3803680981595, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640551, Length=489, Percent_Identity=26.3803680981595, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17737741, Length=501, Percent_Identity=25.9481037924152, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49230; Mature: 49230

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIYTNVAVIGAGTAGLAAYRAARAEGATAVIIEGGPYGTTCARVGCMPSKLLIAAAEA
CCEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCHHHHEEEHHH
AHAASHAAPFGIAVGNVRVDGEAVMARVRRERDRFVGFVVDGVEALPVEDRLVGYARFVD
HHHHHCCCCCEEEECCEEECHHHHHHHHHHHHHHEEEEEECCCEECCCHHHHHHHHHHHC
DGLLQVGDHTQVRAASVVIATGSTPTIPAVLNGVRDRVIVNDDVFAWTTLPRSVAVIGPG
CCCEECCCCCEEEEEEEEEECCCCCCHHHHHHCCCCEEEECCCEEEEECCCCEEEEECCC
VIGLELGQALARLGVRVSVYGARGSVGQLTDPKLVEAARAIFGDAFHFEPSGTMLSATRI
CHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEEC
DDGVTLRYRRADGSTIDATYEYVLAATGRHPDLGNLGLANTSIQLGEHGIPRFDAQTLQV
CCCEEEEEEECCCCEEHHEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEE
QGHPVFIAGDANGILPLLHEAVDEGRAAGRNAARYPDVAKIERRAPLAIVFSDPQIALVG
CCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEECCCCEEEEE
ARHRELRADTFVTGEVSFEDQGRSRVMLKNLGRAHVYVDRATRRFVGAEMLGPSAEHIGH
CCCHHCCCCEEEEEEEEECCCCCCEEHHHHCCCEEEEEEHHHHHHHHHHHCCCCHHHHHH
LLAWSLQMELTVDAMLAMPFYHPVVEEGVRTALMDAAAKLDAPQ
HHHEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTIYTNVAVIGAGTAGLAAYRAARAEGATAVIIEGGPYGTTCARVGCMPSKLLIAAAEA
CCEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCHHHHEEEHHH
AHAASHAAPFGIAVGNVRVDGEAVMARVRRERDRFVGFVVDGVEALPVEDRLVGYARFVD
HHHHHCCCCCEEEECCEEECHHHHHHHHHHHHHHEEEEEECCCEECCCHHHHHHHHHHHC
DGLLQVGDHTQVRAASVVIATGSTPTIPAVLNGVRDRVIVNDDVFAWTTLPRSVAVIGPG
CCCEECCCCCEEEEEEEEEECCCCCCHHHHHHCCCCEEEECCCEEEEECCCCEEEEECCC
VIGLELGQALARLGVRVSVYGARGSVGQLTDPKLVEAARAIFGDAFHFEPSGTMLSATRI
CHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEEC
DDGVTLRYRRADGSTIDATYEYVLAATGRHPDLGNLGLANTSIQLGEHGIPRFDAQTLQV
CCCEEEEEEECCCCEEHHEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEE
QGHPVFIAGDANGILPLLHEAVDEGRAAGRNAARYPDVAKIERRAPLAIVFSDPQIALVG
CCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEECCCCEEEEE
ARHRELRADTFVTGEVSFEDQGRSRVMLKNLGRAHVYVDRATRRFVGAEMLGPSAEHIGH
CCCHHCCCCEEEEEEEEECCCCCCEEHHHHCCCEEEEEEHHHHHHHHHHHCCCCHHHHHH
LLAWSLQMELTVDAMLAMPFYHPVVEEGVRTALMDAAAKLDAPQ
HHHEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]