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The map label for this gene is rutD [H]

Identifier: 189353275

GI number: 189353275

Start: 1513854

End: 1514750

Strand: Reverse

Name: rutD [H]

Synonym: BMULJ_04515

Alternate gene names: 189353275

Gene position: 1514750-1513854 (Counterclockwise)

Preceding gene: 189353276

Following gene: 189353274

Centisome position: 61.25

GC content: 74.36

Gene sequence:

>897_bases
ATGAGTGTCGTCGACAAACGAGAACGCGACCGCACCGCGGCGTTCGCCACGCTGCTCGAGCGGTTTCCCGAGCAGCGCTG
CGCCGCCGGCGCGGCGGGCACGATCGGTTATCGCGAGGCCGGTGCGCAGCATGCCGGCGCCGCGCTGCCCGTCGTGCTGC
TGCACGGGATCGGCTCGGGCGCCGCGTCGTGGGTGCGCCAGCTCGACGCGCTCGGCGCTTCGCGCCGCGTGCTCGCGTGG
GACGCGCCCGGCTACGGCGCATCGACGCCGGTGCACGGCGCATCGCCGTCGGCCGTCGATTACGCGGCGTCGCTCGACGC
GTGGCTCGATGCGCTCGGCATCGCGCGCTGCGTGCTCGTCGGGCATTCGCTCGGCGCGATCGTCGCGGGCGGCATGGTGC
GCGTGAAGCCCGAGCGAATCGCGGGGCTGCTGCTGATTTCGCCGGCCGGCGGTTACGGCAGCGCGCCGGCGGAGACGCGC
GCGTCGCGCCGCGACGCGCGGCTCGCGATGCTCGCGGAACTCGGGCCTGCCGGGCTCGCCGCGCAGCGCAGCGGCAACAT
GCTGTCGGGCTTCGCGACCGAAGACGCGCGCGCATGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCATCTGCTCGCCAATGCCGATCTCGCCGCCGATCTCGCCGGGTTTCGCGGCCGCACGGCGGTGGCCGTCGGC
GCGAACGACGCGATTACGCCGCCCGCGGCGTGCGAGCGCATCGCGGCGGTCGCCGGCGTCGGACTGCAGGTGATTCCGCA
GGCCGGCCACGCGGGCTACGTCGAGGCGCCGGACGTCTATTCGGCCTTGATCGACACGTTCTGCCGGCAGTGCGACCTCG
AGCGGAGGGCGGCATGA

Upstream 100 bases:

>100_bases
GATGTGCGTGATGCTCGGTTCGCCGAAGCCGATCACGCCGACGTATCCGCCCGATTCGCCGCTCGCGAAGATCAAGCGCT
GACACGGAGCGGATGAAGCG

Downstream 100 bases:

>100_bases
GCGAACCGTTGCAGAGCGCGGCCGAGCCCGCGAAGACCGAAGCGAGCTACGTCGTGCCGGGCCTCGAGCGCGGGCTGCGG
ATTCTCGCCGAATTCTCGCC

Product: predicted hydrolase or acyltransferase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQHAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAVAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDLERRAA

Sequences:

>Translated_298_residues
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQHAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAVAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDLERRAA
>Mature_297_residues
SVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQHAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAWD
APGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETRA
SRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVGA
NDAITPPAACERIAAVAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDLERRAA

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

Organism=Homo sapiens, GI50658087, Length=281, Percent_Identity=23.1316725978648, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558492, Length=135, Percent_Identity=30.3703703703704, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32566936, Length=135, Percent_Identity=30.3703703703704, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24586387, Length=114, Percent_Identity=35.0877192982456, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586391, Length=114, Percent_Identity=35.0877192982456, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586389, Length=114, Percent_Identity=35.0877192982456, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586385, Length=279, Percent_Identity=24.3727598566308, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30612; Mature: 30481

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQHAGAALPVVLLHGIGSG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHCCCHHHHHHHHCCCCH
AASWVRQLDALGASRRVLAWDAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLV
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETRASRRDARLAMLAELGPAGLA
HCHHHHHHHCCEEEECHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
AQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
HHCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCCEEEEEC
ANDAITPPAACERIAAVAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDLERRAA
CCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
SVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQHAGAALPVVLLHGIGSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHCCCHHHHHHHHCCCCH
AASWVRQLDALGASRRVLAWDAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLV
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETRASRRDARLAMLAELGPAGLA
HCHHHHHHHCCEEEECHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
AQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
HHCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCCEEEEEC
ANDAITPPAACERIAAVAGVGLQVIPQAGHAGYVEAPDVYSALIDTFCRQCDLERRAA
CCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA