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The map label for this gene is ilvB [H]

Identifier: 189353272

GI number: 189353272

Start: 1510549

End: 1512216

Strand: Reverse

Name: ilvB [H]

Synonym: BMULJ_04512

Alternate gene names: 189353272

Gene position: 1512216-1510549 (Counterclockwise)

Preceding gene: 189353273

Following gene: 189353271

Centisome position: 61.15

GC content: 68.53

Gene sequence:

>1668_bases
ATGTCTAAAAAAACCACGGTTGGCGAGCTGATTGCCGCCTTTCTCGAGCAGTGCGGCGTGAAGACCGCATTCGGTGTCAT
CTCGATCCACAACATGCCGATCCTCGACGCGATCAACACGCGCGGCAACATCCGCTACGTCGGCGCGCGCGGCGAGGCGG
GCGCGGTCAACATGGCCGACGGCCTCGCCCGCGTGTCGGGCGGCCTCGGCGTCGCGTTCACGAGCACCGGCACCGCGGCC
GGCAATGCGGCCGGCGCGATGGTCGAGGCGCTGACGGCCGGCACCGCGCTGCTGCACATCACCGGGCAGATCGAAACGCC
GTACCTCGATCAGGATCTCGCGTATATCCACGAAGCGCCCGATCAGCTGACGATGCTCGACGCGATCTCGAAGGCCGCAT
TCCGCGTGCGGACCGTCGAAACCGTGCTGCCGACGATCCGCGAAGCGGTGCGCATCGCGCAGACCGCGCCGACGGGCCCC
GTGTCGGTCGAGATTCCGATCGACATCCAGGCGGCCGAGATCGAGTGGCCGGAAGACCTCGCGCCCGCGCACGTCGCGGT
GCGCGAGCACGACAGCGAGCGCGTCGCGAAGCTCGCCGACGCACTCGCGTCGAAGCGCCGTCCGCTGCTGTGGCTCGGCG
GCGGCGCGCGGCATGCGCGTGCCGAAGTCGAGCGCTTGGTGAAGCTCGGCTTCGGTGTCGTCACGAGCGTGCAGGGGCGC
GGCGTGCTGCCCGAGGATCATCCGGCGACGCTCGGCGCGTTCAACGTGCATGCGGCCGTCGAAGCGTTCTACAAGACCTG
CGATGCGCTCGTCGTCGTCGGTTCGCGGCTGCGCGGCAACGAAACGCTGAAGTACAAGCTCGCGCTGCCGCAGCCGCTGT
TCCGCATCGACGCGGACGCGCTCGCCGACAACCGCGGCTATCGCAACGCGCTGTTCGTGCACGGCGATTCGAAGCGCGTG
CTCGCCGAGCTCGCCGACCGCCTCGAAGGCCGGCTGTCGGTCGATCCGCAGTTCGCGGTCGATCTGGCCGGCGCGCGTGA
ACAGGCGGTGGCCGACGTGTCGAAGGGGCTCGGGCCGTACAAGCAGCTCGTCGACACGCTGCAGAACGCGGTCGGACGCG
ACTACAACTGGGTGCGCGACGTGACGATCTCGAACAGCACGTGGGGCAACCGGCTGCTGAAGATCTTCGAACCGCGCGCG
GGCGTGCATGCGCTCGGCGGCGGCATCGGCCAGGGCATTCAGATGGGGATCGGCGCGGCGCTCGCGGGCAGCGCGGCGAA
GACGGTGTGCCTCGTCGGCGACGGCGGCCTGATGGTGAACGTCGGCGAGCTCGTCACGGCCGTGCAGGAAAACGCGAACG
TCATGATCGTGCTGATGAACGATCAGTGCTACGGCGTGATCCGCAACATCCAGGACGCGCACTACGGCGGCCGCCGCTGC
TTCGTGCAACTGCACCAGCCCGATTTCGCGCAGTTCTGCGCGAGCTTCGGCCTGACGCACTACCGCATCACGTCGCTCGA
CCAGGCCGAAGCGGTCGTGCGCGAAGGCCTCGCGAAGCAGGGCCCGGTGATGGTCGAAGTCGACATGCTGTCGGTCGGCT
CGTTCGCTTCCGCGTTCGCGGGCCCGCCGGTGAAGAAGGAAGCGCCGGAGGAGCGCCAGTATGCGTAA

Upstream 100 bases:

>100_bases
AAGCCCGACGAGGCCGCCTGGGCGCTTTTTTATCTCGCAACGCATCTGTCGTCCTACACGACGGGCAGCCATATCGACGT
TTCTGGAGGCTTTGCACGCC

Downstream 100 bases:

>100_bases
CGGCCACGCGCACCACGGGCATGGGCCCGTCGACGTCGCGATGATTGGCTTCGGTGCGATCGGCGCGGCCGTCTATCGCG
CGGTCGAGCACGATGCGTCG

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSKKTTVGELIAAFLEQCGVKTAFGVISIHNMPILDAINTRGNIRYVGARGEAGAVNMADGLARVSGGLGVAFTSTGTAA
GNAAGAMVEALTAGTALLHITGQIETPYLDQDLAYIHEAPDQLTMLDAISKAAFRVRTVETVLPTIREAVRIAQTAPTGP
VSVEIPIDIQAAEIEWPEDLAPAHVAVREHDSERVAKLADALASKRRPLLWLGGGARHARAEVERLVKLGFGVVTSVQGR
GVLPEDHPATLGAFNVHAAVEAFYKTCDALVVVGSRLRGNETLKYKLALPQPLFRIDADALADNRGYRNALFVHGDSKRV
LAELADRLEGRLSVDPQFAVDLAGAREQAVADVSKGLGPYKQLVDTLQNAVGRDYNWVRDVTISNSTWGNRLLKIFEPRA
GVHALGGGIGQGIQMGIGAALAGSAAKTVCLVGDGGLMVNVGELVTAVQENANVMIVLMNDQCYGVIRNIQDAHYGGRRC
FVQLHQPDFAQFCASFGLTHYRITSLDQAEAVVREGLAKQGPVMVEVDMLSVGSFASAFAGPPVKKEAPEERQYA

Sequences:

>Translated_555_residues
MSKKTTVGELIAAFLEQCGVKTAFGVISIHNMPILDAINTRGNIRYVGARGEAGAVNMADGLARVSGGLGVAFTSTGTAA
GNAAGAMVEALTAGTALLHITGQIETPYLDQDLAYIHEAPDQLTMLDAISKAAFRVRTVETVLPTIREAVRIAQTAPTGP
VSVEIPIDIQAAEIEWPEDLAPAHVAVREHDSERVAKLADALASKRRPLLWLGGGARHARAEVERLVKLGFGVVTSVQGR
GVLPEDHPATLGAFNVHAAVEAFYKTCDALVVVGSRLRGNETLKYKLALPQPLFRIDADALADNRGYRNALFVHGDSKRV
LAELADRLEGRLSVDPQFAVDLAGAREQAVADVSKGLGPYKQLVDTLQNAVGRDYNWVRDVTISNSTWGNRLLKIFEPRA
GVHALGGGIGQGIQMGIGAALAGSAAKTVCLVGDGGLMVNVGELVTAVQENANVMIVLMNDQCYGVIRNIQDAHYGGRRC
FVQLHQPDFAQFCASFGLTHYRITSLDQAEAVVREGLAKQGPVMVEVDMLSVGSFASAFAGPPVKKEAPEERQYA
>Mature_554_residues
SKKTTVGELIAAFLEQCGVKTAFGVISIHNMPILDAINTRGNIRYVGARGEAGAVNMADGLARVSGGLGVAFTSTGTAAG
NAAGAMVEALTAGTALLHITGQIETPYLDQDLAYIHEAPDQLTMLDAISKAAFRVRTVETVLPTIREAVRIAQTAPTGPV
SVEIPIDIQAAEIEWPEDLAPAHVAVREHDSERVAKLADALASKRRPLLWLGGGARHARAEVERLVKLGFGVVTSVQGRG
VLPEDHPATLGAFNVHAAVEAFYKTCDALVVVGSRLRGNETLKYKLALPQPLFRIDADALADNRGYRNALFVHGDSKRVL
AELADRLEGRLSVDPQFAVDLAGAREQAVADVSKGLGPYKQLVDTLQNAVGRDYNWVRDVTISNSTWGNRLLKIFEPRAG
VHALGGGIGQGIQMGIGAALAGSAAKTVCLVGDGGLMVNVGELVTAVQENANVMIVLMNDQCYGVIRNIQDAHYGGRRCF
VQLHQPDFAQFCASFGLTHYRITSLDQAEAVVREGLAKQGPVMVEVDMLSVGSFASAFAGPPVKKEAPEERQYA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=531, Percent_Identity=25.8003766478343, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI93004078, Length=557, Percent_Identity=24.4165170556553, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1790104, Length=536, Percent_Identity=28.3582089552239, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI87081685, Length=533, Percent_Identity=25.891181988743, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1786717, Length=476, Percent_Identity=26.2605042016807, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=24.0808823529412, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788716, Length=546, Percent_Identity=22.7106227106227, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17542570, Length=569, Percent_Identity=24.6045694200351, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=559, Percent_Identity=24.5080500894454, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320123, Length=539, Percent_Identity=21.1502782931354, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=554, Percent_Identity=22.7436823104693, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323073, Length=543, Percent_Identity=22.6519337016575, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59025; Mature: 58894

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKTTVGELIAAFLEQCGVKTAFGVISIHNMPILDAINTRGNIRYVGARGEAGAVNMAD
CCCCCHHHHHHHHHHHHCCCCHHEEEEEECCCCEEECCCCCCCEEEEECCCCCCCEEHHH
GLARVSGGLGVAFTSTGTAAGNAAGAMVEALTAGTALLHITGQIETPYLDQDLAYIHEAP
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHCCC
DQLTMLDAISKAAFRVRTVETVLPTIREAVRIAQTAPTGPVSVEIPIDIQAAEIEWPEDL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCC
APAHVAVREHDSERVAKLADALASKRRPLLWLGGGARHARAEVERLVKLGFGVVTSVQGR
CCCEEEEHHCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCCC
GVLPEDHPATLGAFNVHAAVEAFYKTCDALVVVGSRLRGNETLKYKLALPQPLFRIDADA
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHCCHHH
LADNRGYRNALFVHGDSKRVLAELADRLEGRLSVDPQFAVDLAGAREQAVADVSKGLGPY
HHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHH
KQLVDTLQNAVGRDYNWVRDVTISNSTWGNRLLKIFEPRAGVHALGGGIGQGIQMGIGAA
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHH
LAGSAAKTVCLVGDGGLMVNVGELVTAVQENANVMIVLMNDQCYGVIRNIQDAHYGGRRC
HCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEE
FVQLHQPDFAQFCASFGLTHYRITSLDQAEAVVREGLAKQGPVMVEVDMLSVGSFASAFA
EEEECCCCHHHHHHHHCCCEEEECCCHHHHHHHHHCHHCCCCEEEEEEEECCCCHHHHHC
GPPVKKEAPEERQYA
CCCCCCCCCHHCCCC
>Mature Secondary Structure 
SKKTTVGELIAAFLEQCGVKTAFGVISIHNMPILDAINTRGNIRYVGARGEAGAVNMAD
CCCCHHHHHHHHHHHHCCCCHHEEEEEECCCCEEECCCCCCCEEEEECCCCCCCEEHHH
GLARVSGGLGVAFTSTGTAAGNAAGAMVEALTAGTALLHITGQIETPYLDQDLAYIHEAP
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHCCC
DQLTMLDAISKAAFRVRTVETVLPTIREAVRIAQTAPTGPVSVEIPIDIQAAEIEWPEDL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCC
APAHVAVREHDSERVAKLADALASKRRPLLWLGGGARHARAEVERLVKLGFGVVTSVQGR
CCCEEEEHHCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCCC
GVLPEDHPATLGAFNVHAAVEAFYKTCDALVVVGSRLRGNETLKYKLALPQPLFRIDADA
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHCCHHH
LADNRGYRNALFVHGDSKRVLAELADRLEGRLSVDPQFAVDLAGAREQAVADVSKGLGPY
HHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHH
KQLVDTLQNAVGRDYNWVRDVTISNSTWGNRLLKIFEPRAGVHALGGGIGQGIQMGIGAA
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHH
LAGSAAKTVCLVGDGGLMVNVGELVTAVQENANVMIVLMNDQCYGVIRNIQDAHYGGRRC
HCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEE
FVQLHQPDFAQFCASFGLTHYRITSLDQAEAVVREGLAKQGPVMVEVDMLSVGSFASAFA
EEEECCCCHHHHHHHHCCCEEEECCCHHHHHHHHHCHHCCCCEEEEEEEECCCCHHHHHC
GPPVKKEAPEERQYA
CCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]