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The map label for this gene is flbD [H]

Identifier: 189353026

GI number: 189353026

Start: 1193513

End: 1194535

Strand: Reverse

Name: flbD [H]

Synonym: BMULJ_04260

Alternate gene names: 189353026

Gene position: 1194535-1193513 (Counterclockwise)

Preceding gene: 189353032

Following gene: 189353017

Centisome position: 48.3

GC content: 70.97

Gene sequence:

>1023_bases
ATGCTGAAGCCGGCCCGGGAAACGTCGTCCCGCGCGAAACGCGCGGACGAACGCCGGCTGTCCGGCCGCTCGGCGGCGAT
GCGCGAGCTGCTGGCGCGTATCGAGAAAGTCGCGCCGACCGGCGCGAGCGTGATGATTGCGGGGGAAAGCGGCGTCGGCA
AGGACATCGTCGCGCGCCGGCTGCACGAGCTCAGCGCGCGGCGCAGCGGGCCGTTCGTCGCGGTCAACTGCGGCGCGATT
CCGCCCGACATCGCGGAGTCGCAGCTGTTCGGCCACGAAAAAGGCAGCTTCACGGGCGCGACCGCGCAGCACACCGGCTT
CTTCGAAGCCGCGCGCGGCGGCACGCTGCTGCTCGACGAAATCGCGGAAATGCCGGCCGCGCTGCAGGTGAAGCTGCTGC
GCGCGCTGGAGTCGAATACGATCCTGCGCGTCGGCGGCACGGAAACGATCGCGCTCGACGTGCGGATCGTGTCCGCGACG
CGCCACAATCCGGCCGACGCCGTGCGCGACGGCCGCCTGCGCGAAGACCTGTTCTACCGGCTCGCCGCGTTTGCGCTCGT
CGTGCCGCCGCTGCGTCAGCGCGAGGGCGACGTCGAGGTGCTCGCGCAGGAATTCGTCGCGGCGCTCAATGCGCGGCACC
GCTCCGACAAGCGGCTGACCGATGCGGCGATCGCCGCGCTGCGTGCGTACTCGTGGCCCGGCAACGTGCGCGAGCTGCGC
AATACGATCGAGCGCGCGTACATCCTCGCGGACGACGGGATCGACGTCACGCTGCCGAAGCAGCCTATGCCGGCCGACCT
GATGCACGACGACGCGATCTCGCTGCCGCTCGGCGCGACGCTGCATCATGCGCAGCAGCGCTTCATCGCGGCGACGCTGA
GCCATTTCGAGGGCAACAAGCCGCTCGCCGCGAAGGCGCTCGGCATCAGCCTGAAGACGCTCTACAACCGGCTCGCGCTG
ATGCGCGAGGCCGGCGACGACGCCGCGCGCCCGGCGTCCGGCGAGCGCGTTACGCGACCCTGA

Upstream 100 bases:

>100_bases
CATTGGAAATTCCTATGCGGCGGCACCTTCCCTTCGCCTGCGTTGTCGATACCCGGTATTGGAAATGGACGAATTGCGTG
ACATCGAAGTGCAAACCGAC

Downstream 100 bases:

>100_bases
ACTGGCCGACCGTCGACGCGAGCGCGTTCGCCTGCGTCTGCAGCGCCGACGCCGCCGCGGTCGACTGTTCGACCAGCGCC
GCGTTCTGCTGAACCATCTC

Product: sigma54-dependent DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MLKPARETSSRAKRADERRLSGRSAAMRELLARIEKVAPTGASVMIAGESGVGKDIVARRLHELSARRSGPFVAVNCGAI
PPDIAESQLFGHEKGSFTGATAQHTGFFEAARGGTLLLDEIAEMPAALQVKLLRALESNTILRVGGTETIALDVRIVSAT
RHNPADAVRDGRLREDLFYRLAAFALVVPPLRQREGDVEVLAQEFVAALNARHRSDKRLTDAAIAALRAYSWPGNVRELR
NTIERAYILADDGIDVTLPKQPMPADLMHDDAISLPLGATLHHAQQRFIAATLSHFEGNKPLAAKALGISLKTLYNRLAL
MREAGDDAARPASGERVTRP

Sequences:

>Translated_340_residues
MLKPARETSSRAKRADERRLSGRSAAMRELLARIEKVAPTGASVMIAGESGVGKDIVARRLHELSARRSGPFVAVNCGAI
PPDIAESQLFGHEKGSFTGATAQHTGFFEAARGGTLLLDEIAEMPAALQVKLLRALESNTILRVGGTETIALDVRIVSAT
RHNPADAVRDGRLREDLFYRLAAFALVVPPLRQREGDVEVLAQEFVAALNARHRSDKRLTDAAIAALRAYSWPGNVRELR
NTIERAYILADDGIDVTLPKQPMPADLMHDDAISLPLGATLHHAQQRFIAATLSHFEGNKPLAAKALGISLKTLYNRLAL
MREAGDDAARPASGERVTRP
>Mature_340_residues
MLKPARETSSRAKRADERRLSGRSAAMRELLARIEKVAPTGASVMIAGESGVGKDIVARRLHELSARRSGPFVAVNCGAI
PPDIAESQLFGHEKGSFTGATAQHTGFFEAARGGTLLLDEIAEMPAALQVKLLRALESNTILRVGGTETIALDVRIVSAT
RHNPADAVRDGRLREDLFYRLAAFALVVPPLRQREGDVEVLAQEFVAALNARHRSDKRLTDAAIAALRAYSWPGNVRELR
NTIERAYILADDGIDVTLPKQPMPADLMHDDAISLPLGATLHHAQQRFIAATLSHFEGNKPLAAKALGISLKTLYNRLAL
MREAGDDAARPASGERVTRP

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=314, Percent_Identity=42.6751592356688, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=40.6153846153846, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1790437, Length=306, Percent_Identity=42.156862745098, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI87082117, Length=230, Percent_Identity=48.2608695652174, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789087, Length=250, Percent_Identity=42, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1788905, Length=307, Percent_Identity=39.413680781759, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1786524, Length=314, Percent_Identity=41.7197452229299, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=46.8085106382979, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1790299, Length=325, Percent_Identity=38.7692307692308, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI87081872, Length=312, Percent_Identity=37.5, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=315, Percent_Identity=37.1428571428571, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=34.8360655737705, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081858, Length=291, Percent_Identity=29.8969072164948, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 36835; Mature: 36835

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPARETSSRAKRADERRLSGRSAAMRELLARIEKVAPTGASVMIAGESGVGKDIVARR
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
LHELSARRSGPFVAVNCGAIPPDIAESQLFGHEKGSFTGATAQHTGFFEAARGGTLLLDE
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHCCCHHHCCCCEEEHHH
IAEMPAALQVKLLRALESNTILRVGGTETIALDVRIVSATRHNPADAVRDGRLREDLFYR
HHHCCHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEEECCCCCHHHHHHCCCHHHHHHHH
LAAFALVVPPLRQREGDVEVLAQEFVAALNARHRSDKRLTDAAIAALRAYSWPGNVRELR
HHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHH
NTIERAYILADDGIDVTLPKQPMPADLMHDDAISLPLGATLHHAQQRFIAATLSHFEGNK
HHHHHEEEEECCCEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PLAAKALGISLKTLYNRLALMREAGDDAARPASGERVTRP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKPARETSSRAKRADERRLSGRSAAMRELLARIEKVAPTGASVMIAGESGVGKDIVARR
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
LHELSARRSGPFVAVNCGAIPPDIAESQLFGHEKGSFTGATAQHTGFFEAARGGTLLLDE
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHCCCHHHCCCCEEEHHH
IAEMPAALQVKLLRALESNTILRVGGTETIALDVRIVSATRHNPADAVRDGRLREDLFYR
HHHCCHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEEECCCCCHHHHHHCCCHHHHHHHH
LAAFALVVPPLRQREGDVEVLAQEFVAALNARHRSDKRLTDAAIAALRAYSWPGNVRELR
HHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHH
NTIERAYILADDGIDVTLPKQPMPADLMHDDAISLPLGATLHHAQQRFIAATLSHFEGNK
HHHHHEEEEECCCEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PLAAKALGISLKTLYNRLALMREAGDDAARPASGERVTRP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]