BacMap

Definition	Burkholderia multivorans ATCC 17616 chromosome 2, complete genome.
Accession	NC_010805
Length	2,473,162

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The map label for this gene is ydfG [C]

Identifier: 189352972

GI number: 189352972

Start: 1132355

End: 1133155

Strand: Reverse

Name: ydfG [C]

Synonym: BMULJ_04206

Alternate gene names: 189352972

Gene position: 1133155-1132355 (Counterclockwise)

Preceding gene: 189352974

Following gene: 189352968

Centisome position: 45.82

GC content: 69.04

Gene sequence:

>801_bases
ATGAGTCAAGTTCATCGCGGTACCGCCGTCGTCACCGGCGCATCGTCCGGCATCGGCGCGATCTATGCGGACCGGCTCGC
GCGCCGCGGCTACGATCTGATCCTCGTCGCGCGCAGCCGCACGCGGCTCGACGCGCTCGCGCAGCGGATCACGAGCGATA
CGCGACGCAGCGTCGAAGTGATCGAGGCCGACCTGAACGACCGCGCCGCGCTCGCCGCCGTCGAAGCGAAGCTGAAGCAG
GACGCGAGCATCACGCTGCTCGTCAACAACGCGGGCATCGGCACGCATACGCCGCTGCTCGAAAGCGACGTCGACGCGAT
GACCCGGATGATCGACCTGAACGTCACGGCGCTCACGCGCCTCACGTATGCGGCAGTGCCCGGCTTCGTCGCGCGCGGGC
ATGGCGCGGTGATCAATATCGCGTCGATCGTCGCGCTGTCGCCGGAAACGCTGAACGGCGTGTACGGTGGCAGCAAGGCG
TTCGTGCTCGCGTTCACGCAGTCGCTGCATCACGAACTCGCGGACAAGGGCGTGCAGGTGCAGGCCGTGCTGCCCGGCGC
GACCGCGACCGATTTCTGGCAGACCGGCGGCCTGCCGATCGAGCATCTGCCGAAGTCGATCGTGATGTCCGCGTCCGACA
TGGTCGATGCCGCGCTGGTCGGCTTCGAGCGGCGCGAACTCGTGACGATTCCGTCGCTGCACGCGGGCGAGGCATGGGAC
GCTTACGAGGCGGCGCGCCGTGCGATGGCGCCGCACCTGTCGTCCGACACACCGGCGCCGCGCTACGCGGCCGCGCGCTG
A

Upstream 100 bases:

>100_bases
CGGCGCGATGTCCTGAATCATACCCATCTACGTCATTTGTGTCGCACGCGTTCGGCGCCAGAATGCCTGCACCCCATCCT
TACTCGAACGGAGCGACATC

Downstream 100 bases:

>100_bases
CGCCGCCCGGCGCCGCGCATCGGCATCAAACGGCCGCCGAGCCGCGCGCGTCCGCGCTGCCGTGCGGCGCGGCGCGCACG
CCGATCAGCATCGCCACGGC

Product: putative short-chain dehydrogenase of various substrate specificities

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSQVHRGTAVVTGASSGIGAIYADRLARRGYDLILVARSRTRLDALAQRITSDTRRSVEVIEADLNDRAALAAVEAKLKQ
DASITLLVNNAGIGTHTPLLESDVDAMTRMIDLNVTALTRLTYAAVPGFVARGHGAVINIASIVALSPETLNGVYGGSKA
FVLAFTQSLHHELADKGVQVQAVLPGATATDFWQTGGLPIEHLPKSIVMSASDMVDAALVGFERRELVTIPSLHAGEAWD
AYEAARRAMAPHLSSDTPAPRYAAAR

Sequences:

>Translated_266_residues
MSQVHRGTAVVTGASSGIGAIYADRLARRGYDLILVARSRTRLDALAQRITSDTRRSVEVIEADLNDRAALAAVEAKLKQ
DASITLLVNNAGIGTHTPLLESDVDAMTRMIDLNVTALTRLTYAAVPGFVARGHGAVINIASIVALSPETLNGVYGGSKA
FVLAFTQSLHHELADKGVQVQAVLPGATATDFWQTGGLPIEHLPKSIVMSASDMVDAALVGFERRELVTIPSLHAGEAWD
AYEAARRAMAPHLSSDTPAPRYAAAR
>Mature_265_residues
SQVHRGTAVVTGASSGIGAIYADRLARRGYDLILVARSRTRLDALAQRITSDTRRSVEVIEADLNDRAALAAVEAKLKQD
ASITLLVNNAGIGTHTPLLESDVDAMTRMIDLNVTALTRLTYAAVPGFVARGHGAVINIASIVALSPETLNGVYGGSKAF
VLAFTQSLHHELADKGVQVQAVLPGATATDFWQTGGLPIEHLPKSIVMSASDMVDAALVGFERRELVTIPSLHAGEAWDA
YEAARRAMAPHLSSDTPAPRYAAAR

Specific function: Unknown

COG id: COG0300

COG function: function code R; Short-chain dehydrogenases of various substrate specificities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7705855, Length=188, Percent_Identity=34.5744680851064, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4557649, Length=186, Percent_Identity=36.0215053763441, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI226371731, Length=185, Percent_Identity=32.4324324324324, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI7706318, Length=187, Percent_Identity=29.9465240641711, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI33667109, Length=190, Percent_Identity=32.1052631578947, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI40254992, Length=199, Percent_Identity=31.1557788944724, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI5031737, Length=190, Percent_Identity=32.1052631578947, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI32483357, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI20149619, Length=189, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI7657478, Length=188, Percent_Identity=28.1914893617021, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI7705925, Length=186, Percent_Identity=29.5698924731183, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI15277342, Length=218, Percent_Identity=31.1926605504587, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI109715829, Length=192, Percent_Identity=29.6875, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI223718074, Length=195, Percent_Identity=29.7435897435897, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI150247226, Length=195, Percent_Identity=30.2564102564103, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787820, Length=186, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082100, Length=200, Percent_Identity=32.5, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787335, Length=185, Percent_Identity=30.2702702702703, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082160, Length=220, Percent_Identity=25.9090909090909, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789208, Length=191, Percent_Identity=31.9371727748691, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786701, Length=190, Percent_Identity=27.3684210526316, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788459, Length=199, Percent_Identity=29.6482412060301, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790717, Length=183, Percent_Identity=29.5081967213115, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367175, Length=211, Percent_Identity=27.9620853080569, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=192, Percent_Identity=38.0208333333333, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994600, Length=193, Percent_Identity=34.7150259067358, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25152911, Length=192, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17557668, Length=189, Percent_Identity=29.1005291005291, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI212646214, Length=190, Percent_Identity=28.9473684210526, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559552, Length=189, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560176, Length=197, Percent_Identity=27.9187817258883, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=209, Percent_Identity=30.1435406698565, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=189, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=215, Percent_Identity=26.5116279069767, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=208, Percent_Identity=29.3269230769231, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=215, Percent_Identity=28.3720930232558, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=244, Percent_Identity=27.4590163934426, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=206, Percent_Identity=27.6699029126214, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=209, Percent_Identity=29.6650717703349, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=198, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=180, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25147288, Length=225, Percent_Identity=27.5555555555556, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=232, Percent_Identity=27.1551724137931, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=200, Percent_Identity=28.5, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=248, Percent_Identity=27.4193548387097, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=186, Percent_Identity=30.6451612903226, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319635, Length=187, Percent_Identity=28.8770053475936, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323882, Length=198, Percent_Identity=32.8282828282828, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322067, Length=214, Percent_Identity=27.1028037383178, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI161076933, Length=186, Percent_Identity=36.0215053763441, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24584748, Length=186, Percent_Identity=36.0215053763441, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24640442, Length=188, Percent_Identity=32.4468085106383, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28574402, Length=188, Percent_Identity=34.0425531914894, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24584744, Length=188, Percent_Identity=34.0425531914894, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28571526, Length=208, Percent_Identity=36.0576923076923, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24584752, Length=186, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357041, Length=195, Percent_Identity=34.3589743589744, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI23397609, Length=193, Percent_Identity=34.7150259067358, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24644339, Length=199, Percent_Identity=31.6582914572864, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI116007236, Length=214, Percent_Identity=30.3738317757009, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI116007238, Length=191, Percent_Identity=30.8900523560209, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648944, Length=219, Percent_Identity=30.1369863013699, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580925, Length=201, Percent_Identity=31.8407960199005, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24665247, Length=197, Percent_Identity=29.4416243654822, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221331216, Length=197, Percent_Identity=29.4416243654822, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358495, Length=197, Percent_Identity=28.9340101522843, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24665243, Length=197, Percent_Identity=29.4416243654822, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221331218, Length=197, Percent_Identity=29.4416243654822, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24639444, Length=181, Percent_Identity=31.4917127071823, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 28188; Mature: 28057

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVHRGTAVVTGASSGIGAIYADRLARRGYDLILVARSRTRLDALAQRITSDTRRSVEV
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
IEADLNDRAALAAVEAKLKQDASITLLVNNAGIGTHTPLLESDVDAMTRMIDLNVTALTR
EECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
LTYAAVPGFVARGHGAVINIASIVALSPETLNGVYGGSKAFVLAFTQSLHHELADKGVQV
HHHHHCCCHHHCCCCCEEEEEHHEEECCHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCEE
QAVLPGATATDFWQTGGLPIEHLPKSIVMSASDMVDAALVGFERRELVTIPSLHAGEAWD
EEEECCCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHH
AYEAARRAMAPHLSSDTPAPRYAAAR
HHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQVHRGTAVVTGASSGIGAIYADRLARRGYDLILVARSRTRLDALAQRITSDTRRSVEV
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
IEADLNDRAALAAVEAKLKQDASITLLVNNAGIGTHTPLLESDVDAMTRMIDLNVTALTR
EECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
LTYAAVPGFVARGHGAVINIASIVALSPETLNGVYGGSKAFVLAFTQSLHHELADKGVQV
HHHHHCCCHHHCCCCCEEEEEHHEEECCHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCEE
QAVLPGATATDFWQTGGLPIEHLPKSIVMSASDMVDAALVGFERRELVTIPSLHAGEAWD
EEEECCCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHH
AYEAARRAMAPHLSSDTPAPRYAAAR
HHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]