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The map label for this gene is cusS [H]

Identifier: 189352956

GI number: 189352956

Start: 1111224

End: 1112681

Strand: Reverse

Name: cusS [H]

Synonym: BMULJ_04190

Alternate gene names: 189352956

Gene position: 1112681-1111224 (Counterclockwise)

Preceding gene: 189352957

Following gene: 189352955

Centisome position: 44.99

GC content: 69.68

Gene sequence:

>1458_bases
ATGAAACGCTCGATCGCGCTGCGGCTGTCGACGATGTTCGGCATCGTGTCGCTGCTCGTCTTTACGCTCGTCGGCTGCGG
GCTGTTCGTGATGATGGAGCGCCAGCTGTTCGCCGAGCTGCGCGCGACGCTCGACACGCGCGCGAAGGTCGCCGAAATGA
TCGTATCGCATGCGCTCACGCCCGAACGCGGCCGGCTGATGAAGGAGAAGCTGGCCGACCTCGAGCCGCCCGACGGCTCG
ACGCGCTATCAGGTCGACACGCCGAATCCGGACTTCCGCTTCGGCCGTCCCGTCGACGGCGTGCCGGTCGGCGCACCGCT
CGGCGCGTTCCAGCGCTACGCGCTCAACGACAGCAGCTACGACGTGATGACGAAAACCGTCACGGTGGCCGGCAGCGGCG
CGCGGCCGACGCTGAAGCTGATCGTCGCGAGCTCGTGCGAGCGCACGCAGCGGATGCTGCGCCGCTTCGGCTGGACGCTC
GCCGCGCTCATCGCAACCGCGACCGTCCTCACGCTGATGCTGAGCCGCGCGGTCGCGCGCTTCGGGCTCGCGCCGCTCGA
CCGGCTGTCGCAGGACGCGGCGGCTGTGAGTGTCGCGAACCGCCGCCAGCGGCTGCAGACCGACGCGCTGCCGGCCGAGC
TGCGCGAACTGGCCGCCTCGTTCAACGGCGCGCTCGAGCGCATCCAGCAGACCTACGAGCGGCTCGAAGCATTCAACGCG
GATGTCGCGCACGAGTTGCGCACGCCGGTCAGCATCCTGATCGGCCAGACGCAGGTCGCGCTGACGAGCCGCGACCGTTC
CGCCGACCGGCTGCGCCAGACGCTGCAGTCGAATCTCGAGGAATTCGAGCGCTTGCGCGTGATCATCAACGACATGCTGT
TCCTGTCGCGCAGCGACCGCGGCGAACGCGCGACCGATCTGAAGTACGTGTCGCTCGCGCACGAGGTGCAGCGCATGCTC
GACTTCCTCGAAATCCCGCTCGACGAAGCGCAACTGCGCGCGGAGCTGCACGGCGACGCGCGCGCGGCCGTCGATCCGTC
GCTGTTCCGGCGCGCGATGACGAACCTGCTGATCAACGCGATCCAGCATTCGGCGCCCGGCGCGACGCTGCACGTGACGA
TCGCGCCGCGCGACGCGCTCGTCGACGTGTCGGTCGCGAACCCTGGCGAGCCGATCGATCCCGCGCGGCGCGCGCATGTG
TTCGAGCGTTTCTACCGGCTCGAGGAAGCGCGCGCGAACAGCATGGAGAATCACGGGCTCGGGCTGTCGATCGTGAAGGC
CGTCGCCGAAATGCACGGCGGCAGCGTGTTCGTCACGTGCGCCGACGGCGTCAACACGTTCGGCTTCTCGGTGTGCGCGC
AACCTTCCGGCGACACACCGGCGCGCGCGCAGGACGCATCCGGCCACCCGGCGTCGGCGACGCTGCCGCACGCGCGTCCG
TCGCGCGCGGCGCACTGA

Upstream 100 bases:

>100_bases
AGCTCGACGGCCCGTTCCCGGAGAAGCTGCTGCATACGATTCGCGGCATGGGCTACGTGCTCGAAGCGCGCGACGACGGC
GGCGACACGGAGAAGCGCAC

Downstream 100 bases:

>100_bases
GTTCGCGCGACGCGCCGACGGCAGTTTTCTTCAATACGCGCACGCGCCGCGCGCTTACTCTCGATGCGACTTCCGACATT
CGCATGGAGAGATTCGATGA

Product: two-component system heavy metal sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKRSIALRLSTMFGIVSLLVFTLVGCGLFVMMERQLFAELRATLDTRAKVAEMIVSHALTPERGRLMKEKLADLEPPDGS
TRYQVDTPNPDFRFGRPVDGVPVGAPLGAFQRYALNDSSYDVMTKTVTVAGSGARPTLKLIVASSCERTQRMLRRFGWTL
AALIATATVLTLMLSRAVARFGLAPLDRLSQDAAAVSVANRRQRLQTDALPAELRELAASFNGALERIQQTYERLEAFNA
DVAHELRTPVSILIGQTQVALTSRDRSADRLRQTLQSNLEEFERLRVIINDMLFLSRSDRGERATDLKYVSLAHEVQRML
DFLEIPLDEAQLRAELHGDARAAVDPSLFRRAMTNLLINAIQHSAPGATLHVTIAPRDALVDVSVANPGEPIDPARRAHV
FERFYRLEEARANSMENHGLGLSIVKAVAEMHGGSVFVTCADGVNTFGFSVCAQPSGDTPARAQDASGHPASATLPHARP
SRAAH

Sequences:

>Translated_485_residues
MKRSIALRLSTMFGIVSLLVFTLVGCGLFVMMERQLFAELRATLDTRAKVAEMIVSHALTPERGRLMKEKLADLEPPDGS
TRYQVDTPNPDFRFGRPVDGVPVGAPLGAFQRYALNDSSYDVMTKTVTVAGSGARPTLKLIVASSCERTQRMLRRFGWTL
AALIATATVLTLMLSRAVARFGLAPLDRLSQDAAAVSVANRRQRLQTDALPAELRELAASFNGALERIQQTYERLEAFNA
DVAHELRTPVSILIGQTQVALTSRDRSADRLRQTLQSNLEEFERLRVIINDMLFLSRSDRGERATDLKYVSLAHEVQRML
DFLEIPLDEAQLRAELHGDARAAVDPSLFRRAMTNLLINAIQHSAPGATLHVTIAPRDALVDVSVANPGEPIDPARRAHV
FERFYRLEEARANSMENHGLGLSIVKAVAEMHGGSVFVTCADGVNTFGFSVCAQPSGDTPARAQDASGHPASATLPHARP
SRAAH
>Mature_485_residues
MKRSIALRLSTMFGIVSLLVFTLVGCGLFVMMERQLFAELRATLDTRAKVAEMIVSHALTPERGRLMKEKLADLEPPDGS
TRYQVDTPNPDFRFGRPVDGVPVGAPLGAFQRYALNDSSYDVMTKTVTVAGSGARPTLKLIVASSCERTQRMLRRFGWTL
AALIATATVLTLMLSRAVARFGLAPLDRLSQDAAAVSVANRRQRLQTDALPAELRELAASFNGALERIQQTYERLEAFNA
DVAHELRTPVSILIGQTQVALTSRDRSADRLRQTLQSNLEEFERLRVIINDMLFLSRSDRGERATDLKYVSLAHEVQRML
DFLEIPLDEAQLRAELHGDARAAVDPSLFRRAMTNLLINAIQHSAPGATLHVTIAPRDALVDVSVANPGEPIDPARRAHV
FERFYRLEEARANSMENHGLGLSIVKAVAEMHGGSVFVTCADGVNTFGFSVCAQPSGDTPARAQDASGHPASATLPHARP
SRAAH

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=311, Percent_Identity=36.3344051446945, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788279, Length=353, Percent_Identity=30.8781869688385, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1790346, Length=265, Percent_Identity=29.0566037735849, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789403, Length=279, Percent_Identity=29.3906810035842, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788393, Length=268, Percent_Identity=26.4925373134328, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082128, Length=326, Percent_Identity=22.3926380368098, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=27.2321428571429, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=215, Percent_Identity=25.5813953488372, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787894, Length=272, Percent_Identity=28.3088235294118, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790551, Length=279, Percent_Identity=25.8064516129032, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=25.8389261744966, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081816, Length=257, Percent_Identity=27.6264591439689, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53154; Mature: 53154

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSIALRLSTMFGIVSLLVFTLVGCGLFVMMERQLFAELRATLDTRAKVAEMIVSHALT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PERGRLMKEKLADLEPPDGSTRYQVDTPNPDFRFGRPVDGVPVGAPLGAFQRYALNDSSY
CHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DVMTKTVTVAGSGARPTLKLIVASSCERTQRMLRRFGWTLAALIATATVLTLMLSRAVAR
EEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLAPLDRLSQDAAAVSVANRRQRLQTDALPAELRELAASFNGALERIQQTYERLEAFNA
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVAHELRTPVSILIGQTQVALTSRDRSADRLRQTLQSNLEEFERLRVIINDMLFLSRSDR
HHHHHHCCCHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GERATDLKYVSLAHEVQRMLDFLEIPLDEAQLRAELHGDARAAVDPSLFRRAMTNLLINA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHH
IQHSAPGATLHVTIAPRDALVDVSVANPGEPIDPARRAHVFERFYRLEEARANSMENHGL
HHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLSIVKAVAEMHGGSVFVTCADGVNTFGFSVCAQPSGDTPARAQDASGHPASATLPHARP
HHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
SRAAH
CCCCC
>Mature Secondary Structure
MKRSIALRLSTMFGIVSLLVFTLVGCGLFVMMERQLFAELRATLDTRAKVAEMIVSHALT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PERGRLMKEKLADLEPPDGSTRYQVDTPNPDFRFGRPVDGVPVGAPLGAFQRYALNDSSY
CHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DVMTKTVTVAGSGARPTLKLIVASSCERTQRMLRRFGWTLAALIATATVLTLMLSRAVAR
EEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLAPLDRLSQDAAAVSVANRRQRLQTDALPAELRELAASFNGALERIQQTYERLEAFNA
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVAHELRTPVSILIGQTQVALTSRDRSADRLRQTLQSNLEEFERLRVIINDMLFLSRSDR
HHHHHHCCCHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GERATDLKYVSLAHEVQRMLDFLEIPLDEAQLRAELHGDARAAVDPSLFRRAMTNLLINA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHH
IQHSAPGATLHVTIAPRDALVDVSVANPGEPIDPARRAHVFERFYRLEEARANSMENHGL
HHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLSIVKAVAEMHGGSVFVTCADGVNTFGFSVCAQPSGDTPARAQDASGHPASATLPHARP
HHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
SRAAH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]