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The map label for this gene is dadA [C]

Identifier: 189352829

GI number: 189352829

Start: 952422

End: 953549

Strand: Direct

Name: dadA [C]

Synonym: BMULJ_04063

Alternate gene names: 189352829

Gene position: 952422-953549 (Clockwise)

Preceding gene: 189352828

Following gene: 189352830

Centisome position: 38.51

GC content: 72.87

Gene sequence:

>1128_bases
GTGAGCGAATCGGCAACCGACGTCGTCGTGATCGGCGCGGGCATCGTCGGCGCCGCGTGCGCGCACGAGCTCGCGCAGCG
CGGGCTGCGCGTGATCGTCGTCGACGATGCGAGCGGTGGCGCGACGGGGGCCGGCATGGGGCACCTGGTCGCGATGGACG
ACAACGCAGCCGAACTCGCGCTCAGCCATTACTCGATCGAACTGTGGCGCGCACTGAGCGGCGACATGCCGGACGGCTGC
GCGTACCGCAACTGCGGCACGCTGTGGCTCGCGGCGGACGCGCACGAGATGGATCTCGCGCGCGCGAAGCAGGCGACGCT
GGCCGCGCACGGCGTGGCGGGCGAACTGCTCGACGCGGCCGCGCTCGCGCGGCTCGAGCCGATGCTGCGGCCCGGCCTCG
GCGGCGCGCTGAAGATTCCCGGCGACGGCATTCTCTATGCGCCGGTCGCCGCGAACTGGCTGCTGCAGCGCGGGCCCGGC
ATCGTGCTGCGGCGCGACCGCGCGGTCGACGTCGACGGCGCGCGCGTCACGCTCGCGAGCGGCGACGTGCTGCGTGCGCA
ACGCGTCGTCGTCGCGAACGGTGTTGCCGCGCGTACGCTGCTGCCCGAACTGCCGCTGCGCCCGAAGAAGGGCCATCTGC
TGATTACCGACCGTTATCCGGGGCGCGTCGCGCACCAGCTCGTCGAGCTCGGCTATGCGGCGAGCGCGCATGCGAGCGAC
GGCACGTCGGTCGCGTTCAACGTGCAGCCGCGGCCGACGGGCCAGCTGCTGATCGGATCGTCGCGCCAGTTCGACACCGA
GGATCCGCGCATCGAGCCGCCGGTGCTCGCGCGGATGCTGCGCCGCGCGGCCGGCTACCTGCCCGATCTCGCCGAGCTGA
ACGCGATCCGCGCATGGACGGGCTTCCGTGCCGCGAGCCCGGATGGCCTGCCGCTGCTCGGCGAACATCCGGCGCGGCCC
GGCGTATGGCTCGCCGTGGGCCACGAAGGGCTCGGCGTGACGACCGCGCCGGGCAGTGCGCGGCTGCTGGCCGCGCAGAT
GGCGGGCGAGCGGCCCGCGATCGACATCGAACCGTATTTGCCGGGACGTTTTCTGACGACGTCCCCTGTAGCCGGAGCGC
TTTCATGA

Upstream 100 bases:

>100_bases
AAGGCGGCATCGTGCCGACGATTCGCGGCAGCGCGCATCTGAGCGCCGAAGCGACGCTGCTGATCGAGGACGACGATCCG
TTCCGCTGGGGCATCGTGTC

Downstream 100 bases:

>100_bases
TCATTCATCTCGACGGCCGCGCGCTGACGGTGGCGGACGGCGTGACCGTCGCGGCCGCCGTGGCGGCGAGCGGCGACGAC
ACGACGCGCGTGTCGTGCAC

Product: glycine/D-amino acid oxidase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSESATDVVVIGAGIVGAACAHELAQRGLRVIVVDDASGGATGAGMGHLVAMDDNAAELALSHYSIELWRALSGDMPDGC
AYRNCGTLWLAADAHEMDLARAKQATLAAHGVAGELLDAAALARLEPMLRPGLGGALKIPGDGILYAPVAANWLLQRGPG
IVLRRDRAVDVDGARVTLASGDVLRAQRVVVANGVAARTLLPELPLRPKKGHLLITDRYPGRVAHQLVELGYAASAHASD
GTSVAFNVQPRPTGQLLIGSSRQFDTEDPRIEPPVLARMLRRAAGYLPDLAELNAIRAWTGFRAASPDGLPLLGEHPARP
GVWLAVGHEGLGVTTAPGSARLLAAQMAGERPAIDIEPYLPGRFLTTSPVAGALS

Sequences:

>Translated_375_residues
MSESATDVVVIGAGIVGAACAHELAQRGLRVIVVDDASGGATGAGMGHLVAMDDNAAELALSHYSIELWRALSGDMPDGC
AYRNCGTLWLAADAHEMDLARAKQATLAAHGVAGELLDAAALARLEPMLRPGLGGALKIPGDGILYAPVAANWLLQRGPG
IVLRRDRAVDVDGARVTLASGDVLRAQRVVVANGVAARTLLPELPLRPKKGHLLITDRYPGRVAHQLVELGYAASAHASD
GTSVAFNVQPRPTGQLLIGSSRQFDTEDPRIEPPVLARMLRRAAGYLPDLAELNAIRAWTGFRAASPDGLPLLGEHPARP
GVWLAVGHEGLGVTTAPGSARLLAAQMAGERPAIDIEPYLPGRFLTTSPVAGALS
>Mature_374_residues
SESATDVVVIGAGIVGAACAHELAQRGLRVIVVDDASGGATGAGMGHLVAMDDNAAELALSHYSIELWRALSGDMPDGCA
YRNCGTLWLAADAHEMDLARAKQATLAAHGVAGELLDAAALARLEPMLRPGLGGALKIPGDGILYAPVAANWLLQRGPGI
VLRRDRAVDVDGARVTLASGDVLRAQRVVVANGVAARTLLPELPLRPKKGHLLITDRYPGRVAHQLVELGYAASAHASDG
TSVAFNVQPRPTGQLLIGSSRQFDTEDPRIEPPVLARMLRRAAGYLPDLAELNAIRAWTGFRAASPDGLPLLGEHPARPG
VWLAVGHEGLGVTTAPGSARLLAAQMAGERPAIDIEPYLPGRFLTTSPVAGALS

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=333, Percent_Identity=27.9279279279279, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20130091, Length=380, Percent_Identity=23.4210526315789, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39048; Mature: 38917

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESATDVVVIGAGIVGAACAHELAQRGLRVIVVDDASGGATGAGMGHLVAMDDNAAELA
CCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHEE
LSHYSIELWRALSGDMPDGCAYRNCGTLWLAADAHEMDLARAKQATLAAHGVAGELLDAA
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ALARLEPMLRPGLGGALKIPGDGILYAPVAANWLLQRGPGIVLRRDRAVDVDGARVTLAS
HHHHHHHHHCCCCCCEEECCCCCEEECCHHHHHHHHCCCCEEEECCCEECCCCCEEEEEC
GDVLRAQRVVVANGVAARTLLPELPLRPKKGHLLITDRYPGRVAHQLVELGYAASAHASD
CCEEHHCEEEEECCCHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCC
GTSVAFNVQPRPTGQLLIGSSRQFDTEDPRIEPPVLARMLRRAAGYLPDLAELNAIRAWT
CCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
GFRAASPDGLPLLGEHPARPGVWLAVGHEGLGVTTAPGSARLLAAQMAGERPAIDIEPYL
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCEEEEEHHCCCCCCEEECCCC
PGRFLTTSPVAGALS
CCCEEECCCCCCCCC
>Mature Secondary Structure 
SESATDVVVIGAGIVGAACAHELAQRGLRVIVVDDASGGATGAGMGHLVAMDDNAAELA
CCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHEE
LSHYSIELWRALSGDMPDGCAYRNCGTLWLAADAHEMDLARAKQATLAAHGVAGELLDAA
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ALARLEPMLRPGLGGALKIPGDGILYAPVAANWLLQRGPGIVLRRDRAVDVDGARVTLAS
HHHHHHHHHCCCCCCEEECCCCCEEECCHHHHHHHHCCCCEEEECCCEECCCCCEEEEEC
GDVLRAQRVVVANGVAARTLLPELPLRPKKGHLLITDRYPGRVAHQLVELGYAASAHASD
CCEEHHCEEEEECCCHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCC
GTSVAFNVQPRPTGQLLIGSSRQFDTEDPRIEPPVLARMLRRAAGYLPDLAELNAIRAWT
CCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
GFRAASPDGLPLLGEHPARPGVWLAVGHEGLGVTTAPGSARLLAAQMAGERPAIDIEPYL
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCEEEEEHHCCCCCCEEECCCC
PGRFLTTSPVAGALS
CCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]