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The map label for this gene is puuB [H]

Identifier: 189352752

GI number: 189352752

Start: 861015

End: 862424

Strand: Direct

Name: puuB [H]

Synonym: BMULJ_03984

Alternate gene names: 189352752

Gene position: 861015-862424 (Clockwise)

Preceding gene: 189352751

Following gene: 189352753

Centisome position: 34.81

GC content: 68.51

Gene sequence:

>1410_bases
ATGTCGACCATCGAACGCACCGCGAGCGGCTTTGCGGCCGGACGTCAGCACGACACGCGCTACGATCCGACCTACGATCC
GCTCGTGTCGCCCGGCCCCGGCTGCGGCAAGCAGTACGCGCCGACCTACTGGGTCGCGACGGCCGGCAGCCCGCCGCCCG
ACGACGGCTCCGTCACGCGCGACATGGACGTCGACGTCGCGATCATCGGCGCCGGCTACACGGGCCTCGCGACCGCGCTG
TGCCTCGCCCGCGACCACGGCATCAAGGCGGTCGTGCTCGAAGCGAACCGCACGAGCTGGGGCTGCAGCAGCCGCAACGG
CGGGCAGGGGCAGAACGCGTCGGGCCGCCTGTCGCGCTCGCAGTGGATCGAGCGCTGGGGCAAGGACGTCGCGCTGAAGA
TGCACGACGAGATCCTCGAAGGCTTCGAGCACTTCAAGTCGCTCGTCGCCGAAATCGACTGCGATCCGCAGCCCGGCGGC
CACTTCCTGATCGCGCATCGTCCGCGCATCATGGCGAAGCTCGCGGCCGAGGCGAAGGTATGGAAGGACGTGTTCGGCTA
TCCGTCCGAGCTGCTGAGCGCGGAGACGTTCCGCCGCGAATTCCTCAACGATCATGAAGCGGCCGGCGCGCTGCACGAGC
CCGAAGGGATCGGCATTCATGCGCTGAAGCTCGCGTTCGGCTATCTGCGCCTCGCGCGCGAAGCGGGCGCGAAGGTCTAC
ACGAGCAGCCCGGTGCTCGGCTTCGAGACGATCGGCGGCGTGCATCATCTGCGTACGCCGGGCGGTGTCGTGCGTGCGCG
CGCGGTCGGCATCGCGACCGGCGCGTATACGGCGCAGACGCTCCATCCGTCGCTGCGCAGCAAGGTGATGCCGATCCTGT
CCAATTCGATGGTCACGCGTCCGCTGACGGACGCGGAGATCGAGGCGTGCAATTTCCGCACGACGCAGGTGCTGACCGAC
ACGCGCACGCTGCGCTTCTACTACCGGTTTCTGCCCGATCGCCGGCTGCAGATCGGCAGCCGCAGCGCGATCACCGGCAG
CGATGCGCCGAACCCGCGTCACCTCGATCTGCTCAAGGAAGGCATGGCGCGCAAGTTTCCCGCGCTGCGCGGCGTGCAGA
TCGACTACTCGTGGTGGGGCTGGGTCGACGTCAGTCACGACATGATGCCGCGCGTCTGCCAGCCCGATCCGCATCAGTCG
GTGTTCTACGCGCTCGGCTACGGCGGCAACGGCGTGTCGTACTCGCAGCAGGCCGGCCGGCGGCTCGCCGAGCGGATCGC
CGGCAAGGGCGAGCGTCAGACGCTGCCGATCTTCACGTCGCCACTGCCCGGTCATCCGTTCGCGCCGTTCCGGCGCATCG
GGCAACGCATGCTCTACGTGTCGTACTTCAGGCGCGACGAGAAGCCCTGA

Upstream 100 bases:

>100_bases
CATGCGCCCGTGCGGCGGATGCGCGCCCCGCAACCGTCAGTCGAATCGCCGTCGGCGCGCCGTGCGGCGTCGCGGCCGCC
ACCGTGTCGGAGGACTGACC

Downstream 100 bases:

>100_bases
GCGTGACCGTGCGCGGTGCACCGCGGACACCGCGGGCCCGCTCGACCGAATAACCCTCGACCGAATAACAACAGCAAGAC
CGATCCGTGCGGCCGGGGTC

Product: glycine/D-amino acid oxidase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSTIERTASGFAAGRQHDTRYDPTYDPLVSPGPGCGKQYAPTYWVATAGSPPPDDGSVTRDMDVDVAIIGAGYTGLATAL
CLARDHGIKAVVLEANRTSWGCSSRNGGQGQNASGRLSRSQWIERWGKDVALKMHDEILEGFEHFKSLVAEIDCDPQPGG
HFLIAHRPRIMAKLAAEAKVWKDVFGYPSELLSAETFRREFLNDHEAAGALHEPEGIGIHALKLAFGYLRLAREAGAKVY
TSSPVLGFETIGGVHHLRTPGGVVRARAVGIATGAYTAQTLHPSLRSKVMPILSNSMVTRPLTDAEIEACNFRTTQVLTD
TRTLRFYYRFLPDRRLQIGSRSAITGSDAPNPRHLDLLKEGMARKFPALRGVQIDYSWWGWVDVSHDMMPRVCQPDPHQS
VFYALGYGGNGVSYSQQAGRRLAERIAGKGERQTLPIFTSPLPGHPFAPFRRIGQRMLYVSYFRRDEKP

Sequences:

>Translated_469_residues
MSTIERTASGFAAGRQHDTRYDPTYDPLVSPGPGCGKQYAPTYWVATAGSPPPDDGSVTRDMDVDVAIIGAGYTGLATAL
CLARDHGIKAVVLEANRTSWGCSSRNGGQGQNASGRLSRSQWIERWGKDVALKMHDEILEGFEHFKSLVAEIDCDPQPGG
HFLIAHRPRIMAKLAAEAKVWKDVFGYPSELLSAETFRREFLNDHEAAGALHEPEGIGIHALKLAFGYLRLAREAGAKVY
TSSPVLGFETIGGVHHLRTPGGVVRARAVGIATGAYTAQTLHPSLRSKVMPILSNSMVTRPLTDAEIEACNFRTTQVLTD
TRTLRFYYRFLPDRRLQIGSRSAITGSDAPNPRHLDLLKEGMARKFPALRGVQIDYSWWGWVDVSHDMMPRVCQPDPHQS
VFYALGYGGNGVSYSQQAGRRLAERIAGKGERQTLPIFTSPLPGHPFAPFRRIGQRMLYVSYFRRDEKP
>Mature_468_residues
STIERTASGFAAGRQHDTRYDPTYDPLVSPGPGCGKQYAPTYWVATAGSPPPDDGSVTRDMDVDVAIIGAGYTGLATALC
LARDHGIKAVVLEANRTSWGCSSRNGGQGQNASGRLSRSQWIERWGKDVALKMHDEILEGFEHFKSLVAEIDCDPQPGGH
FLIAHRPRIMAKLAAEAKVWKDVFGYPSELLSAETFRREFLNDHEAAGALHEPEGIGIHALKLAFGYLRLAREAGAKVYT
SSPVLGFETIGGVHHLRTPGGVVRARAVGIATGAYTAQTLHPSLRSKVMPILSNSMVTRPLTDAEIEACNFRTTQVLTDT
RTLRFYYRFLPDRRLQIGSRSAITGSDAPNPRHLDLLKEGMARKFPALRGVQIDYSWWGWVDVSHDMMPRVCQPDPHQSV
FYALGYGGNGVSYSQQAGRRLAERIAGKGERQTLPIFTSPLPGHPFAPFRRIGQRMLYVSYFRRDEKP

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=414, Percent_Identity=33.3333333333333, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51720; Mature: 51589

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIERTASGFAAGRQHDTRYDPTYDPLVSPGPGCGKQYAPTYWVATAGSPPPDDGSVTR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEE
DMDVDVAIIGAGYTGLATALCLARDHGIKAVVLEANRTSWGCSSRNGGQGQNASGRLSRS
CCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
QWIERWGKDVALKMHDEILEGFEHFKSLVAEIDCDPQPGGHFLIAHRPRIMAKLAAEAKV
HHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHH
WKDVFGYPSELLSAETFRREFLNDHEAAGALHEPEGIGIHALKLAFGYLRLAREAGAKVY
HHHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
TSSPVLGFETIGGVHHLRTPGGVVRARAVGIATGAYTAQTLHPSLRSKVMPILSNSMVTR
ECCCCCCHHHHCCHHEECCCCCCEEHHHEEEECCCCHHHHHCHHHHHHHHHHHHCCHHCC
PLTDAEIEACNFRTTQVLTDTRTLRFYYRFLPDRRLQIGSRSAITGSDAPNPRHLDLLKE
CCCCCHHHHCCCEEHEEHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHH
GMARKFPALRGVQIDYSWWGWVDVSHDMMPRVCQPDPHQSVFYALGYGGNGVSYSQQAGR
HHHHHCCCCCCEEEECCEEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
RLAERIAGKGERQTLPIFTSPLPGHPFAPFRRIGQRMLYVSYFRRDEKP
HHHHHHHCCCCCEECCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STIERTASGFAAGRQHDTRYDPTYDPLVSPGPGCGKQYAPTYWVATAGSPPPDDGSVTR
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEE
DMDVDVAIIGAGYTGLATALCLARDHGIKAVVLEANRTSWGCSSRNGGQGQNASGRLSRS
CCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
QWIERWGKDVALKMHDEILEGFEHFKSLVAEIDCDPQPGGHFLIAHRPRIMAKLAAEAKV
HHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHH
WKDVFGYPSELLSAETFRREFLNDHEAAGALHEPEGIGIHALKLAFGYLRLAREAGAKVY
HHHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
TSSPVLGFETIGGVHHLRTPGGVVRARAVGIATGAYTAQTLHPSLRSKVMPILSNSMVTR
ECCCCCCHHHHCCHHEECCCCCCEEHHHEEEECCCCHHHHHCHHHHHHHHHHHHCCHHCC
PLTDAEIEACNFRTTQVLTDTRTLRFYYRFLPDRRLQIGSRSAITGSDAPNPRHLDLLKE
CCCCCHHHHCCCEEHEEHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHH
GMARKFPALRGVQIDYSWWGWVDVSHDMMPRVCQPDPHQSVFYALGYGGNGVSYSQQAGR
HHHHHCCCCCCEEEECCEEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
RLAERIAGKGERQTLPIFTSPLPGHPFAPFRRIGQRMLYVSYFRRDEKP
HHHHHHHCCCCCEECCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]