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The map label for this gene is shiA [H]

Identifier: 189352751

GI number: 189352751

Start: 859303

End: 860607

Strand: Direct

Name: shiA [H]

Synonym: BMULJ_03983

Alternate gene names: 189352751

Gene position: 859303-860607 (Clockwise)

Preceding gene: 189352750

Following gene: 189352752

Centisome position: 34.75

GC content: 68.35

Gene sequence:

>1305_bases
ATGGCAATCGATTCCCGGGTCGTCCGCCGCGTGGCCGCGGCCAGCGTGATCGGCGCGACGGTCGAGTGGTACGACTTTTT
CCTGTACGGCGTCGTCGCCGGCATCGTGTTCGACAAGCAGTATTTCCCGGCCGACGATGCGTTCGTGTCGACGATGCTCG
CGTATGCGACGTTCGCGGTCGGCTTCATCACGCGGCCGCTCGGCGGCCTCTTGTTCGGTCATCTTGGCGACCGCGTCGGC
CGCAAGTCCGCGCTGATTCTGACGATGCTGATCATGGGCGTGTCGACGGCCGGCGTCGCGTTCCTGCCCACCTATGCGCA
GATCGGCATGTGGGCGCCCGCGCTGCTGCTGACGCTGCGCGTGCTGCAGGGAATCGGCCTCGGCGGCGAGTGGGGCGGCG
CGGTGCTGATGGCGTACGAATACGCGCCGCCGCATCGGCGCGGGCTCTATGCGAGCCTGCCGCAGATCGGGCTCGCGATC
GGGCTGTGTCTCGCGGCCGGCGTCGTCGCCGCGCTGTCGCGCGTGCTGCCGGACGCCGCATTCCTGTCGTGGGGCTGGCG
CATCGCGTTCGCGGCATCGCTGCTGCTCGTCGCGGTCGGCTTCTACATCCGCACGCGCGTGGCCGATACGCCCGAGTTCG
TCGCGCTGAAACGGCAGCGGCGCGACACGAAGCTGCCGGTCGTCGAGCTGCTCGGCAGCTACCGTCGCGCGATCCTGCTC
GGCATGGGCGCGCGCTACATCGATGGCGTGTTCTTCAACGTGTTCGCGGTGTTCTCGATCGGCTACATGACGCGCAATCT
GTCGATCTCGCGCGGCGACGCGCTGCTCGGCGTGATGAGCGCGGCGGTCGTGATGTGCGCGTTCATTCCCGTGTTCGGCA
GCCTGTCCGACCGGCTCGGCCGAGCGCGCGTGTACCGCTGGGGCGCGACGCTTTGCGGGCTGTCGGTGCTGCCGGCGTTC
TGGCTGCTGCACGCGCATTCGACGAACCTGCTCGCGGTGTGGCTCGCGCTCGTGATTCCGTTCGGGATTTTCTATGCGAT
GGCGTACGGGCCCGAGGCCGCGCTGTTTGCGGAGCTGTTCGACGCGAACGTCCGCTATACGGGCATTTCGTTCGTCTATC
AGGTGTCGGGCATCTTCGCGAGCGGCATCACGCCGATCGTCGCGACCGCGCTGCTGCGTGCGAACGGCGGCGAGCCGTGG
CTCGTCGGGGTTTACGTGATGCTCTCCGCACTGCTGTCGATCGTGTCCGCGCGCGCGATCGAGAAAAGCGGCGGTGTGGC
CGGCGTCGCGGTGCGCGCGACCTGA

Upstream 100 bases:

>100_bases
CCGTGCTCCTGCGCCCGGACGGCCGCCGCGCGCTCATCGGATGCCCCCGCCGGTACGGCGTCGGGGCAGGTCGCGGCGCA
TCCACCCTGAAGGAGCTCGC

Downstream 100 bases:

>100_bases
TCGCCGCGCGCCTGCGGCCGGCGGCGCGCGACATCGCTGCGGCGCATGCCGCAGGCCGTACGCGGTCGCATCCGCGCAAT
CGCCTTGCGCGGCCGCGCGT

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MAIDSRVVRRVAAASVIGATVEWYDFFLYGVVAGIVFDKQYFPADDAFVSTMLAYATFAVGFITRPLGGLLFGHLGDRVG
RKSALILTMLIMGVSTAGVAFLPTYAQIGMWAPALLLTLRVLQGIGLGGEWGGAVLMAYEYAPPHRRGLYASLPQIGLAI
GLCLAAGVVAALSRVLPDAAFLSWGWRIAFAASLLLVAVGFYIRTRVADTPEFVALKRQRRDTKLPVVELLGSYRRAILL
GMGARYIDGVFFNVFAVFSIGYMTRNLSISRGDALLGVMSAAVVMCAFIPVFGSLSDRLGRARVYRWGATLCGLSVLPAF
WLLHAHSTNLLAVWLALVIPFGIFYAMAYGPEAALFAELFDANVRYTGISFVYQVSGIFASGITPIVATALLRANGGEPW
LVGVYVMLSALLSIVSARAIEKSGGVAGVAVRAT

Sequences:

>Translated_434_residues
MAIDSRVVRRVAAASVIGATVEWYDFFLYGVVAGIVFDKQYFPADDAFVSTMLAYATFAVGFITRPLGGLLFGHLGDRVG
RKSALILTMLIMGVSTAGVAFLPTYAQIGMWAPALLLTLRVLQGIGLGGEWGGAVLMAYEYAPPHRRGLYASLPQIGLAI
GLCLAAGVVAALSRVLPDAAFLSWGWRIAFAASLLLVAVGFYIRTRVADTPEFVALKRQRRDTKLPVVELLGSYRRAILL
GMGARYIDGVFFNVFAVFSIGYMTRNLSISRGDALLGVMSAAVVMCAFIPVFGSLSDRLGRARVYRWGATLCGLSVLPAF
WLLHAHSTNLLAVWLALVIPFGIFYAMAYGPEAALFAELFDANVRYTGISFVYQVSGIFASGITPIVATALLRANGGEPW
LVGVYVMLSALLSIVSARAIEKSGGVAGVAVRAT
>Mature_433_residues
AIDSRVVRRVAAASVIGATVEWYDFFLYGVVAGIVFDKQYFPADDAFVSTMLAYATFAVGFITRPLGGLLFGHLGDRVGR
KSALILTMLIMGVSTAGVAFLPTYAQIGMWAPALLLTLRVLQGIGLGGEWGGAVLMAYEYAPPHRRGLYASLPQIGLAIG
LCLAAGVVAALSRVLPDAAFLSWGWRIAFAASLLLVAVGFYIRTRVADTPEFVALKRQRRDTKLPVVELLGSYRRAILLG
MGARYIDGVFFNVFAVFSIGYMTRNLSISRGDALLGVMSAAVVMCAFIPVFGSLSDRLGRARVYRWGATLCGLSVLPAFW
LLHAHSTNLLAVWLALVIPFGIFYAMAYGPEAALFAELFDANVRYTGISFVYQVSGIFASGITPIVATALLRANGGEPWL
VGVYVMLSALLSIVSARAIEKSGGVAGVAVRAT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=417, Percent_Identity=38.1294964028777, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=39.3483709273183, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1790550, Length=432, Percent_Identity=31.4814814814815, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1788942, Length=426, Percent_Identity=31.924882629108, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=25.1121076233184, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082404, Length=219, Percent_Identity=24.6575342465753, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46437; Mature: 46306

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDSRVVRRVAAASVIGATVEWYDFFLYGVVAGIVFDKQYFPADDAFVSTMLAYATFAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GFITRPLGGLLFGHLGDRVGRKSALILTMLIMGVSTAGVAFLPTYAQIGMWAPALLLTLR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VLQGIGLGGEWGGAVLMAYEYAPPHRRGLYASLPQIGLAIGLCLAAGVVAALSRVLPDAA
HHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FLSWGWRIAFAASLLLVAVGFYIRTRVADTPEFVALKRQRRDTKLPVVELLGSYRRAILL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GMGARYIDGVFFNVFAVFSIGYMTRNLSISRGDALLGVMSAAVVMCAFIPVFGSLSDRLG
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RARVYRWGATLCGLSVLPAFWLLHAHSTNLLAVWLALVIPFGIFYAMAYGPEAALFAELF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DANVRYTGISFVYQVSGIFASGITPIVATALLRANGGEPWLVGVYVMLSALLSIVSARAI
CCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EKSGGVAGVAVRAT
HHCCCEEEEEEEEC
>Mature Secondary Structure 
AIDSRVVRRVAAASVIGATVEWYDFFLYGVVAGIVFDKQYFPADDAFVSTMLAYATFAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH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HHCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]