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The map label for this gene is leuB

Identifier: 189352653

GI number: 189352653

Start: 740743

End: 741810

Strand: Direct

Name: leuB

Synonym: BMULJ_03884

Alternate gene names: 189352653

Gene position: 740743-741810 (Clockwise)

Preceding gene: 189352652

Following gene: 189352654

Centisome position: 29.95

GC content: 66.48

Gene sequence:

>1068_bases
ATGAAGATTGCAGTGCTGCCGGGCGACGGCATCGGTCCGGAAATCGTCACCGAAGCGGTGAAGGTGCTGAACGCACTCGA
CGAGAAGTTCGAACTCGAACAGGCGCCGGTCGGCGGCGCGGGCTACGAGGCGAGGGGTCATCCGCTGCCGGACGCGACGC
TGAAGCTCGCGAAGGAAGCCGACGCGATCCTGTTCGGCGCGGTCGGCGACTGGAAGTACGACTCGCTCGAACGCGCGCTG
CGTCCCGAGCAGGCGATTCTCGGCCTGCGCAAGCACCTCGAGCTGTTCGCGAACTTCCGTCCGGCGATCTGCTATCCGCA
GCTCGTCGACGCGTCGCCGCTGAAGCCCGAACTTGTCGCGGGCCTCGACATCCTGATCGTGCGCGAACTGAACGGCGACA
TCTATTTCGGCCAGCCGCGCGGCGTGCGCGCGGCACCGGACGGCCCGTTCGCGGGCGAGCGCGAAGGCTTCGACACGATG
CGCTATTCGGAGCCGGAAGTGCGCCGCATCGCGCACGTCGCGTTCCAGGCCGCGCAAAAGCGCGCGAAGAAGCTGCTGTC
GGTCGACAAGTCGAACGTGCTCGAGACGTCGCAGTTCTGGCGCGACATCATGATCGACGTGTCGAAGGAATATGCGGACG
TCGAGCTGTCGCACATGTACGTCGACAACGCGGCGATGCAGCTCGCGAAGGCGCCGAAGCAGTTCGACGTGATCGTGACC
GGCAACATGTTCGGCGACATCCTGTCGGATGAAGCGTCGATGCTGACGGGCTCGATCGGCATGCTGCCGTCGGCGTCGCT
CGACAAGAACAACAAGGGTCTGTACGAGCCGTCGCACGGTTCGGCGCCGGACATCGCGGGCAAGGGCATCGCGAATCCGC
TCGCGACGATCCTGTCGGCCGCGATGCTGCTGCGCTATTCGCTGAACCGCGCCGAGCAGGCCGACCGCATCGAGCGCGCG
GTCAAAACGGTGCTCGAACAGGGCTACCGCACCGGCGACATCGCGACGCCGGGCTGCAAGCAGGTCGGCACGGCGGCGAT
GGGCGACGCGGTGGTCGCGGCCCTCTGA

Upstream 100 bases:

>100_bases
CTCAACAACAAGCTGGTCGGCTGAAGTCGTCCTTCGGGCGGGCGCGCACGCCGCGCGTTCGCCCGCCAGCCATCACCCCT
TATTCAGTCAGGAACAACGC

Downstream 100 bases:

>100_bases
GCGGCCGGCAAGTCACGATGTAGAAAGGGCGCGAACGGGCCGAAAAAAATCGGTTCGTTCGCGCACGGTTGGCCGTTGTG
CGGGCAACCGGCTTTGTGTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIAVLPGDGIGPEIVTEAVKVLNALDEKFELEQAPVGGAGYEARGHPLPDATLKLAKEADAILFGAVGDWKYDSLERAL
RPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVAGLDILIVRELNGDIYFGQPRGVRAAPDGPFAGEREGFDTM
RYSEPEVRRIAHVAFQAAQKRAKKLLSVDKSNVLETSQFWRDIMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
GNMFGDILSDEASMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERA
VKTVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL

Sequences:

>Translated_355_residues
MKIAVLPGDGIGPEIVTEAVKVLNALDEKFELEQAPVGGAGYEARGHPLPDATLKLAKEADAILFGAVGDWKYDSLERAL
RPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVAGLDILIVRELNGDIYFGQPRGVRAAPDGPFAGEREGFDTM
RYSEPEVRRIAHVAFQAAQKRAKKLLSVDKSNVLETSQFWRDIMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
GNMFGDILSDEASMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERA
VKTVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL
>Mature_355_residues
MKIAVLPGDGIGPEIVTEAVKVLNALDEKFELEQAPVGGAGYEARGHPLPDATLKLAKEADAILFGAVGDWKYDSLERAL
RPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVAGLDILIVRELNGDIYFGQPRGVRAAPDGPFAGEREGFDTM
RYSEPEVRRIAHVAFQAAQKRAKKLLSVDKSNVLETSQFWRDIMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
GNMFGDILSDEASMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGIANPLATILSAAMLLRYSLNRAEQADRIERA
VKTVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=329, Percent_Identity=30.6990881458967, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4758582, Length=340, Percent_Identity=26.7647058823529, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI28178838, Length=322, Percent_Identity=27.0186335403727, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI28178821, Length=307, Percent_Identity=26.0586319218241, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI28178816, Length=307, Percent_Identity=26.0586319218241, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI28178819, Length=141, Percent_Identity=31.9148936170213, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=53.3707865168539, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788101, Length=347, Percent_Identity=34.0057636887608, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=25.8793969849246, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=350, Percent_Identity=30, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25144293, Length=342, Percent_Identity=30.1169590643275, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=356, Percent_Identity=28.3707865168539, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17550882, Length=340, Percent_Identity=29.1176470588235, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=46.3013698630137, Blast_Score=285, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=37.7840909090909, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6324291, Length=338, Percent_Identity=28.9940828402367, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324709, Length=363, Percent_Identity=29.7520661157025, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24643268, Length=348, Percent_Identity=32.183908045977, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24643270, Length=348, Percent_Identity=32.183908045977, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24661184, Length=347, Percent_Identity=29.6829971181556, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078635, Length=342, Percent_Identity=26.6081871345029, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI161078637, Length=342, Percent_Identity=26.6081871345029, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078633, Length=342, Percent_Identity=26.6081871345029, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650122, Length=342, Percent_Identity=26.6081871345029, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078639, Length=341, Percent_Identity=26.6862170087977, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281362242, Length=363, Percent_Identity=27.8236914600551, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648872, Length=363, Percent_Identity=27.8236914600551, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20130355, Length=172, Percent_Identity=29.6511627906977, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_BURM1 (Q845W3)

Other databases:

- EMBL:   AB091435
- EMBL:   CP000869
- EMBL:   AP009386
- RefSeq:   YP_001584597.1
- RefSeq:   YP_001948280.1
- ProteinModelPortal:   Q845W3
- SMR:   Q845W3
- GeneID:   5769022
- GeneID:   6362226
- GenomeReviews:   AP009386_GR
- GenomeReviews:   CP000869_GR
- KEGG:   bmj:BMULJ_03884
- KEGG:   bmu:Bmul_4627
- HOGENOM:   HBG518924
- OMA:   NTMIYSE
- ProtClustDB:   PRK00772
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38332; Mature: 38332

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 90-90 BINDING 100-100 BINDING 128-128 BINDING 222-222

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLPGDGIGPEIVTEAVKVLNALDEKFELEQAPVGGAGYEARGHPLPDATLKLAKEA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHC
DAILFGAVGDWKYDSLERALRPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVA
CEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHC
GLDILIVRELNGDIYFGQPRGVRAAPDGPFAGEREGFDTMRYSEPEVRRIAHVAFQAAQK
CCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RAKKLLSVDKSNVLETSQFWRDIMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEHHHEEHHHHHHHHHHCCCCEEEEEE
GNMFGDILSDEASMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGIANPLATILSA
CCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMLLRYSLNRAEQADRIERAVKTVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHCCHHHHCC
>Mature Secondary Structure
MKIAVLPGDGIGPEIVTEAVKVLNALDEKFELEQAPVGGAGYEARGHPLPDATLKLAKEA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHC
DAILFGAVGDWKYDSLERALRPEQAILGLRKHLELFANFRPAICYPQLVDASPLKPELVA
CEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHC
GLDILIVRELNGDIYFGQPRGVRAAPDGPFAGEREGFDTMRYSEPEVRRIAHVAFQAAQK
CCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RAKKLLSVDKSNVLETSQFWRDIMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEHHHEEHHHHHHHHHHCCCCEEEEEE
GNMFGDILSDEASMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGIANPLATILSA
CCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMLLRYSLNRAEQADRIERAVKTVLEQGYRTGDIATPGCKQVGTAAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231