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The map label for this gene is shiA [H]

Identifier: 189352525

GI number: 189352525

Start: 588112

End: 589440

Strand: Reverse

Name: shiA [H]

Synonym: BMULJ_03753

Alternate gene names: 189352525

Gene position: 589440-588112 (Counterclockwise)

Preceding gene: 189352526

Following gene: 189352524

Centisome position: 23.83

GC content: 58.62

Gene sequence:

>1329_bases
ATGGACACACCGAATTCAAACGTCGCCGAGCGCGACACTCGGGTAACGGCCGATCTGCACGCTGATCCGGTGTCTACGTC
ACACCCCATGCGCCGTCTGGCGCTGGCGAGCATGATCGGCACGACACTCGAATGGTATGACTTTGCCCTCTACAACGCTA
TGGCGGCACTGATCTTCAATCGCGTTTTCTTTCCCTCCTTCAGCCCCTTGGCTGGGACGTTGATGGCTTTCTCAACTTAT
GCCGTCGGCTATTTTGCGCGCCCGATCGGGGGTACGATATTCGGCAGGCTTGGTGATCGCCTCGGGCGTCGTGACGTATT
GACGCTGACCCTCACGATTATGGGTGCCACCACTTTCCTTATGAGTCTGTTACCCGGATATGCAACGATCGGCGTGGCAA
GTCCCCTGCTGCTGGTAACACTGCGCTTCATACAGGGGGTCGCATTGGGTGGGGAATGGGCCGGCGCAGTGCTGTTGTCC
GCGGAACACGGTGCACATGCCAATCGCGGGCTGAACGGATCCTGGGCGCAGGTTGGTCCATCTGCCGGAACGCTGCTGGC
CACTGCGGGCATCGGATTGACGACCTGGTGTTTCACGGACAGCGATTTCGTTTCCTGGGGCTGGCGCGTTCCCTTTGTCG
TGAGCGCCGTCCTTGTCGTGTTTGGCCTTTGGCTTCGTCGAAGCGTTCCCGAGACGCCGGACTTTGCGAAACTGAAAGCA
GGCAATGAAACGGCACAGTTTCCCATTACAGAGGTATTCACCTCTCACAAAAGAGCACTTCTGATCGCATGCATGTCGAA
GTTCGGTCCCGACGTCATTTACAATCTGAATGTCACCTTCAGCCTGACCTACGCTACACAGATTCTTCACCTCGGTAGAA
CTGCGGCGCTTATTGCCGTCTGGATCGGCGCAGCATTAAACGCTGTCGCGGTACCATACTTCGGTGCCCTGTCGGACCGC
CTCGGCCGTCGCGCCGTATACGGTGCCGGAATCGCTGCAGCCGCGCTATGGACTTTCTCGTTCTTCCCGCTGCTCAATTC
AGGGTGGCAGTCCGCGCTCGTCATCGGTGTCGCCGTGGGCCTGATTTGCCACGCAGCAATGTTCGGCCCACAGGCTGCCT
TCATAACCGAGCAGTTTCCCACGCGTGTCCGCTACACCGGCGCATCGCTTGCCGTGACGCTCGCCGGCCTGCTTAGCGGA
TCCACACCAGTGATATTGCTCACACTGTTGACGACCTTTCATAGCCCAATCCCGCTCTCACTCTACGCGACAGCGCTACT
CGTGCTTAGCGGAGCAAGTCTGATTCTCGGTCGTCGGAAACCGGGCTAG

Upstream 100 bases:

>100_bases
ACGGCATTGGAGGCGACGCGAACGGCAATCTCTTCGTGGCAGCGCAAGGACCTTCGCGCGTCGTACGCCTGGAACGGTTG
GACAACGAGGAGTGACCACC

Downstream 100 bases:

>100_bases
CCGGAAAAATTTTGTTCGTCACAATCCAATGCATTATTATTTATAAGCACTACGATATAATATGAAGTCATCAAAATTTC
TGACTATTGCCACAATCTTT

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MDTPNSNVAERDTRVTADLHADPVSTSHPMRRLALASMIGTTLEWYDFALYNAMAALIFNRVFFPSFSPLAGTLMAFSTY
AVGYFARPIGGTIFGRLGDRLGRRDVLTLTLTIMGATTFLMSLLPGYATIGVASPLLLVTLRFIQGVALGGEWAGAVLLS
AEHGAHANRGLNGSWAQVGPSAGTLLATAGIGLTTWCFTDSDFVSWGWRVPFVVSAVLVVFGLWLRRSVPETPDFAKLKA
GNETAQFPITEVFTSHKRALLIACMSKFGPDVIYNLNVTFSLTYATQILHLGRTAALIAVWIGAALNAVAVPYFGALSDR
LGRRAVYGAGIAAAALWTFSFFPLLNSGWQSALVIGVAVGLICHAAMFGPQAAFITEQFPTRVRYTGASLAVTLAGLLSG
STPVILLTLLTTFHSPIPLSLYATALLVLSGASLILGRRKPG

Sequences:

>Translated_442_residues
MDTPNSNVAERDTRVTADLHADPVSTSHPMRRLALASMIGTTLEWYDFALYNAMAALIFNRVFFPSFSPLAGTLMAFSTY
AVGYFARPIGGTIFGRLGDRLGRRDVLTLTLTIMGATTFLMSLLPGYATIGVASPLLLVTLRFIQGVALGGEWAGAVLLS
AEHGAHANRGLNGSWAQVGPSAGTLLATAGIGLTTWCFTDSDFVSWGWRVPFVVSAVLVVFGLWLRRSVPETPDFAKLKA
GNETAQFPITEVFTSHKRALLIACMSKFGPDVIYNLNVTFSLTYATQILHLGRTAALIAVWIGAALNAVAVPYFGALSDR
LGRRAVYGAGIAAAALWTFSFFPLLNSGWQSALVIGVAVGLICHAAMFGPQAAFITEQFPTRVRYTGASLAVTLAGLLSG
STPVILLTLLTTFHSPIPLSLYATALLVLSGASLILGRRKPG
>Mature_442_residues
MDTPNSNVAERDTRVTADLHADPVSTSHPMRRLALASMIGTTLEWYDFALYNAMAALIFNRVFFPSFSPLAGTLMAFSTY
AVGYFARPIGGTIFGRLGDRLGRRDVLTLTLTIMGATTFLMSLLPGYATIGVASPLLLVTLRFIQGVALGGEWAGAVLLS
AEHGAHANRGLNGSWAQVGPSAGTLLATAGIGLTTWCFTDSDFVSWGWRVPFVVSAVLVVFGLWLRRSVPETPDFAKLKA
GNETAQFPITEVFTSHKRALLIACMSKFGPDVIYNLNVTFSLTYATQILHLGRTAALIAVWIGAALNAVAVPYFGALSDR
LGRRAVYGAGIAAAALWTFSFFPLLNSGWQSALVIGVAVGLICHAAMFGPQAAFITEQFPTRVRYTGASLAVTLAGLLSG
STPVILLTLLTTFHSPIPLSLYATALLVLSGASLILGRRKPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=389, Percent_Identity=40.3598971722365, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1789941, Length=396, Percent_Identity=37.8787878787879, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790550, Length=420, Percent_Identity=31.4285714285714, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788942, Length=443, Percent_Identity=28.8939051918736, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI87082404, Length=420, Percent_Identity=22.3809523809524, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082231, Length=186, Percent_Identity=25.2688172043011, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47024; Mature: 47024

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPNSNVAERDTRVTADLHADPVSTSHPMRRLALASMIGTTLEWYDFALYNAMAALIFN
CCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVFFPSFSPLAGTLMAFSTYAVGYFARPIGGTIFGRLGDRLGRRDVLTLTLTIMGATTFL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
MSLLPGYATIGVASPLLLVTLRFIQGVALGGEWAGAVLLSAEHGAHANRGLNGSWAQVGP
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHCCC
SAGTLLATAGIGLTTWCFTDSDFVSWGWRVPFVVSAVLVVFGLWLRRSVPETPDFAKLKA
CCCCEEEECCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
GNETAQFPITEVFTSHKRALLIACMSKFGPDVIYNLNVTFSLTYATQILHLGRTAALIAV
CCCCCCCCHHHHHHHCHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHCHHHHHHHH
WIGAALNAVAVPYFGALSDRLGRRAVYGAGIAAAALWTFSFFPLLNSGWQSALVIGVAVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LICHAAMFGPQAAFITEQFPTRVRYTGASLAVTLAGLLSGSTPVILLTLLTTFHSPIPLS
HHHHHHHCCCCHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
LYATALLVLSGASLILGRRKPG
HHHHHHHHHCCCHHEECCCCCC
>Mature Secondary Structure
MDTPNSNVAERDTRVTADLHADPVSTSHPMRRLALASMIGTTLEWYDFALYNAMAALIFN
CCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVFFPSFSPLAGTLMAFSTYAVGYFARPIGGTIFGRLGDRLGRRDVLTLTLTIMGATTFL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
MSLLPGYATIGVASPLLLVTLRFIQGVALGGEWAGAVLLSAEHGAHANRGLNGSWAQVGP
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHCCC
SAGTLLATAGIGLTTWCFTDSDFVSWGWRVPFVVSAVLVVFGLWLRRSVPETPDFAKLKA
CCCCEEEECCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
GNETAQFPITEVFTSHKRALLIACMSKFGPDVIYNLNVTFSLTYATQILHLGRTAALIAV
CCCCCCCCHHHHHHHCHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHCHHHHHHHH
WIGAALNAVAVPYFGALSDRLGRRAVYGAGIAAAALWTFSFFPLLNSGWQSALVIGVAVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LICHAAMFGPQAAFITEQFPTRVRYTGASLAVTLAGLLSGSTPVILLTLLTTFHSPIPLS
HHHHHHHCCCCHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
LYATALLVLSGASLILGRRKPG
HHHHHHHHHCCCHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]