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The map label for this gene is pilJ [H]

Identifier: 189352517

GI number: 189352517

Start: 575709

End: 577256

Strand: Direct

Name: pilJ [H]

Synonym: BMULJ_03740

Alternate gene names: 189352517

Gene position: 575709-577256 (Clockwise)

Preceding gene: 189352505

Following gene: 189352518

Centisome position: 23.28

GC content: 55.81

Gene sequence:

>1548_bases
ATGAGACGGCTGGGATTTCGGCAACAAATGTGGATTCCACTTGTTCTGTCACTAATTTGCCTGGTCGCGGTGGCCACCTT
CGGTGTGTTGCAGAATCGTGTTTCCAGCCTTCGTGAGCGCGAACTCACGCTTAGCCAAGCCGCGGACTTTGCAACGGGCA
TCACGCAGCACTATGCAGACCTTGCTGGCCGGGGAGTCATGAGTGTGGCCGAAGCCAGGGCGGCGGCTTTGACTCAGCTT
CGAGAGGTAAGGTTCGGCAAAGACGGATATATCGCAGTTATCGATCCAGGGATGCATTCGGTTGTGAACCCCTCCAAGCC
GGAGACAGAGGGACGGTACCTGGGTGACTACCGGGACGAAAACGGTCACTACATTTATCGCGAGATGGTCGCCGTCGCTG
AACATGGTCAGGAAGGGCTCGTTGACTACTATACTCATCGACCCGGGGCTACGGAGATCGTCAGGAAGCGGAGCTATGTA
AAGGCATACAGACCATGGAACCTGATATTTGTTACTGGTGCTTATCTGGACGATATTGACAGAGCGTTTGCCAGATCTGC
GCTAGTGACGGTGATATTCGTTGTGGTTACAGGGTTGGTTTTATCAGGTGTCGTTCTCCTGGTTAACCGCGTGCTTATGC
GCATGTTGGGCGGATCGCCCGAGTACGCAAAGGCGATCGTACATGCGACGGCGACGGGTGACCTCTCGATCGGTATCGCA
AGACACCCAAACGACAGCGACAGCTTGATTGCGGGTATCGCGGACATGCAAGCACGGTTGCGTGCCCTTATTGGCAGGAT
TCAGCAAGGGGCTGATGCGATAACCCTATCCATGGAGGAGGTCGCTGCCGGCAACGCCGATCTTGCTCAGCGTACGGAGG
AACAGGCTGCCGCGCTTCAGCAGACCTCCGCCACGATGGCTGGGCTTCGTGATGCCATACAGCGAACAGCATCGGATGCA
AAGTCGGCAGCCTTCTGTGCGGAAGACGCATTTTCGAGCGTTCGGCGCGGCAGTCAGGAAGTCGTGGCTGTTGCGACGAC
GATGGAACGCATCACCGAAAGCTCGAACGAAATCGCCGACATTCTCATTATGATAGAGAGCATTTCGTTTCAGACGAACA
TCCTGGCTCTCAATGCGGCAGTCGAGGCTGCGCGGGCTCAGGAGCACGGACGAGGCTTCGCCGTCGTCGCCTCTGAAGTT
CGTGCATTGGCACAGAGGTCGTCGACGGCTTCGAAAGATATCGAATCGCTAATCAGTCAATCGGATGTGCACGTGAACGA
AGGATCAAGGCTGGCCGACGGCGCGAACCAATGCATGGCACAGATCCTCGATACGGTCCACAAGGTAGATGAACTTGTAA
AAAACATTGCCGGAGAATGCGAGGTTCAGGGGGCTGGAGTGACTGAGATATCGAAGGCGCTTAGTCAAATAGAGGAGGTC
ACGCAGCGCAATGCTGCGCTTGTCGAGCAGTCCGCTGCAGGGGCGCAACTCGTGCATGAGAAGGCACGAGAGCTCGTATC
GAGTGTTTCTGCATTCCGGCTTGTTTAG

Upstream 100 bases:

>100_bases
CGTAGCGGAATCTCGGTTTAAGTAATTATTTGATTTCTAATATTAAAAACGAAGACGATTGGCCGTAAACCAGTCCACAC
ATACATAAACAGGGGATCAA

Downstream 100 bases:

>100_bases
GCATCGGTGTGCGGACGGTGGATCATGGCAGCAATGCAGAGAGTCGCATAGGCTGGCAGTCTATCGCACTGCCTTGGCAA
AGGCACCGGTTCGGTGGCTG

Product: twitching motility protein

Products: NA

Alternate protein names: MCP-III; Ribose and galactose chemoreceptor protein [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MRRLGFRQQMWIPLVLSLICLVAVATFGVLQNRVSSLRERELTLSQAADFATGITQHYADLAGRGVMSVAEARAAALTQL
REVRFGKDGYIAVIDPGMHSVVNPSKPETEGRYLGDYRDENGHYIYREMVAVAEHGQEGLVDYYTHRPGATEIVRKRSYV
KAYRPWNLIFVTGAYLDDIDRAFARSALVTVIFVVVTGLVLSGVVLLVNRVLMRMLGGSPEYAKAIVHATATGDLSIGIA
RHPNDSDSLIAGIADMQARLRALIGRIQQGADAITLSMEEVAAGNADLAQRTEEQAAALQQTSATMAGLRDAIQRTASDA
KSAAFCAEDAFSSVRRGSQEVVAVATTMERITESSNEIADILIMIESISFQTNILALNAAVEAARAQEHGRGFAVVASEV
RALAQRSSTASKDIESLISQSDVHVNEGSRLADGANQCMAQILDTVHKVDELVKNIAGECEVQGAGVTEISKALSQIEEV
TQRNAALVEQSAAGAQLVHEKARELVSSVSAFRLV

Sequences:

>Translated_515_residues
MRRLGFRQQMWIPLVLSLICLVAVATFGVLQNRVSSLRERELTLSQAADFATGITQHYADLAGRGVMSVAEARAAALTQL
REVRFGKDGYIAVIDPGMHSVVNPSKPETEGRYLGDYRDENGHYIYREMVAVAEHGQEGLVDYYTHRPGATEIVRKRSYV
KAYRPWNLIFVTGAYLDDIDRAFARSALVTVIFVVVTGLVLSGVVLLVNRVLMRMLGGSPEYAKAIVHATATGDLSIGIA
RHPNDSDSLIAGIADMQARLRALIGRIQQGADAITLSMEEVAAGNADLAQRTEEQAAALQQTSATMAGLRDAIQRTASDA
KSAAFCAEDAFSSVRRGSQEVVAVATTMERITESSNEIADILIMIESISFQTNILALNAAVEAARAQEHGRGFAVVASEV
RALAQRSSTASKDIESLISQSDVHVNEGSRLADGANQCMAQILDTVHKVDELVKNIAGECEVQGAGVTEISKALSQIEEV
TQRNAALVEQSAAGAQLVHEKARELVSSVSAFRLV
>Mature_515_residues
MRRLGFRQQMWIPLVLSLICLVAVATFGVLQNRVSSLRERELTLSQAADFATGITQHYADLAGRGVMSVAEARAAALTQL
REVRFGKDGYIAVIDPGMHSVVNPSKPETEGRYLGDYRDENGHYIYREMVAVAEHGQEGLVDYYTHRPGATEIVRKRSYV
KAYRPWNLIFVTGAYLDDIDRAFARSALVTVIFVVVTGLVLSGVVLLVNRVLMRMLGGSPEYAKAIVHATATGDLSIGIA
RHPNDSDSLIAGIADMQARLRALIGRIQQGADAITLSMEEVAAGNADLAQRTEEQAAALQQTSATMAGLRDAIQRTASDA
KSAAFCAEDAFSSVRRGSQEVVAVATTMERITESSNEIADILIMIESISFQTNILALNAAVEAARAQEHGRGFAVVASEV
RALAQRSSTASKDIESLISQSDVHVNEGSRLADGANQCMAQILDTVHKVDELVKNIAGECEVQGAGVTEISKALSQIEEV
TQRNAALVEQSAAGAQLVHEKARELVSSVSAFRLV

Specific function: Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=328, Percent_Identity=42.0731707317073, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1788194, Length=335, Percent_Identity=37.910447761194, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788195, Length=266, Percent_Identity=44.7368421052632, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI2367378, Length=328, Percent_Identity=41.7682926829268, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789453, Length=274, Percent_Identity=35.036496350365, Blast_Score=148, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR003122
- InterPro:   IPR004091
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal; PF02203 TarH [H]

EC number: NA

Molecular weight: Translated: 55512; Mature: 55512

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLGFRQQMWIPLVLSLICLVAVATFGVLQNRVSSLRERELTLSQAADFATGITQHYAD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGRGVMSVAEARAAALTQLREVRFGKDGYIAVIDPGMHSVVNPSKPETEGRYLGDYRDE
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCCCCCC
NGHYIYREMVAVAEHGQEGLVDYYTHRPGATEIVRKRSYVKAYRPWNLIFVTGAYLDDID
CCCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
RAFARSALVTVIFVVVTGLVLSGVVLLVNRVLMRMLGGSPEYAKAIVHATATGDLSIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCEEEEEE
RHPNDSDSLIAGIADMQARLRALIGRIQQGADAITLSMEEVAAGNADLAQRTEEQAAALQ
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHH
QTSATMAGLRDAIQRTASDAKSAAFCAEDAFSSVRRGSQEVVAVATTMERITESSNEIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHH
ILIMIESISFQTNILALNAAVEAARAQEHGRGFAVVASEVRALAQRSSTASKDIESLISQ
HHHHHHHHCHHHHHEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCC
SDVHVNEGSRLADGANQCMAQILDTVHKVDELVKNIAGECEVQGAGVTEISKALSQIEEV
CCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHH
TQRNAALVEQSAAGAQLVHEKARELVSSVSAFRLV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRLGFRQQMWIPLVLSLICLVAVATFGVLQNRVSSLRERELTLSQAADFATGITQHYAD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGRGVMSVAEARAAALTQLREVRFGKDGYIAVIDPGMHSVVNPSKPETEGRYLGDYRDE
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCCCCCC
NGHYIYREMVAVAEHGQEGLVDYYTHRPGATEIVRKRSYVKAYRPWNLIFVTGAYLDDID
CCCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
RAFARSALVTVIFVVVTGLVLSGVVLLVNRVLMRMLGGSPEYAKAIVHATATGDLSIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCEEEEEE
RHPNDSDSLIAGIADMQARLRALIGRIQQGADAITLSMEEVAAGNADLAQRTEEQAAALQ
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHH
QTSATMAGLRDAIQRTASDAKSAAFCAEDAFSSVRRGSQEVVAVATTMERITESSNEIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHH
ILIMIESISFQTNILALNAAVEAARAQEHGRGFAVVASEVRALAQRSSTASKDIESLISQ
HHHHHHHHCHHHHHEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCC
SDVHVNEGSRLADGANQCMAQILDTVHKVDELVKNIAGECEVQGAGVTEISKALSQIEEV
CCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHH
TQRNAALVEQSAAGAQLVHEKARELVSSVSAFRLV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6374654; 9097039; 9278503 [H]