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The map label for this gene is dnaG [H]

Identifier: 189352483

GI number: 189352483

Start: 533071

End: 534939

Strand: Direct

Name: dnaG [H]

Synonym: BMULJ_03702

Alternate gene names: 189352483

Gene position: 533071-534939 (Clockwise)

Preceding gene: 189352482

Following gene: 189352484

Centisome position: 21.55

GC content: 66.88

Gene sequence:

>1869_bases
GTGATTCCGCATTCGTTCCTGCAGGATTTGCTGAACCGCGTCGATATCGTCGACGTGGTGGGCCGGTACGTGCAGCTCAA
GAAGGGCGGCGCGAACTTCATGGGGCTGTGCCCGTTCCATAACGAGAAGAGTCCGTCGTTCACCGTTAGCCCGACGAAGC
AGTTCTATCACTGCTTCGGCTGCGGCGCACACGGCACGGCGATCGGCTTTCTGATGGAACACGCGGGGCTGACGTTCCCG
GAAGCCGTGCAGGAACTCGCGCAGTCGGTCGGGCTGACCGTGCCGCACGAGCCGTCGACACGCGGCGGCGGCGGAGCGGG
CGGCGACTATCCGGCGCCCGTATCGAAATCGGTGGCGACGGCGCTCTCCGACGTGATGGCGACCGCGTGCGACTACTACC
GCAAGCAGTTGCGCGGCGCGCCGGCCGCGATCCAGTATCTGAAGAACCGGGGCCTGACCGGCGAGATCGCCGCGCGCTTC
GGGCTCGGCTATGCGCCGGACGGCTGGCAGAACCTCGAGGCCGCGTTCCCGAACTACCGCGACGATGCGCTCGTCGAGTC
CGGGCTCGTGATCGTCAGCGAGAAAACCGACGCGCAGGGCGCCGCGCGCCGCTACGACCGGTTCCGCGAGCGGATCATGT
TCCCGATCCGCAACGTGAAGGGGCAGGTGATCGGCTTCGGCGGCCGCGTGCTCGGCAGCGGCGAACCGAAGTATCTGAAC
TCGCCCGAAACGCCGCTGTTCAACAAGGGCAGCGAACTGTATGGGCTGTTCGAGGCGCGGCTCGCGATCCGCGAACGCAA
GTACGTGCTGGTCGTCGAAGGCTATATGGACGTCGTGGCGCTCGCGCAGCTCGGCTTCCCGAACGCGGTCGCGACGCTCG
GCACCGCCTGCACGCCGATTCACGTGCAGAAGCTGTTGCGGCAGACCGATACGGTGATTTTCAGCTTCGACGGCGATTCG
GCCGGCCGGCGCGCCGCGCGTCGCGCGCTGGAGGCCTGCCTGCCGCACGCGGCGGACAATCGGACGATCCGATTCCTTTT
TCTGCCGCCCGAGCACGATCCGGACAGCTATGTGCGCGAGTTCGGCGCGGACGCGTTCTCGGACCAGGTCGAGCGCGCGA
TGCCGCTGTCGCAATTTCTGTTGAACGAAGCAATTGCGGGCAAGGCGCTCGACCAGCCGGAAGGCCGCGCGAAGGCGCTG
TTCGACGCGAAGCCGCTGCTGCAGGCGCTGCCCGCGAATGCGCTGCGCGCGCAGATCATGCACATGATCGCGGATCGCCT
CGATATTCCGTTCGAGGAAGTGGCGGCGCTGTCGGAAGTCGATGCGCGGATCGCGGCTGCGCCGCGCCAGGCGCCGGCGC
GCAGCGATCGCCGGCGCGTGACGGAGAGCGAAAAGCGCGCGTTGCGCACGCTCGTCATGCATCCGCGCATCGCGCTGGCG
CTCGACGACGAGCAGGTCGCGACGCTGCGCGCGCTGCCGCGCATCGGCGAACTCTTTGCGGAAGTCGTCGACCACGCGCG
CGCGCTCGGCGACGGCGCGGAGTTCCGGCTGCTGTCCGACGTGCTGCGTACGTCGCCGAACAGCGCGACGTACGAGGAAA
TTTTCCGCGAAATTCTGGACTATGATGAAAACGTCCGCGATTTGCTGTTACAGAATCCGGACGACGAGGCGGTGGCCGAG
CGGCAGCGCGAACAGGAACGGATCGCCGGGGAGGAGCTGCAGGCGGCCGTGCTCAAGATGCGCTACGACGCGTGCTGCGA
CCGGCTCGATCGGCTGGCGCGGCAATCCGCGTTCACGCCCGAGGAATTGGCCGAATTGAAGCACTTGAATGAGCAGCGAA
CCGACATGAAACGTCGGCTCGGGCTGTAG

Upstream 100 bases:

>100_bases
TCCGCGCAGGTCAAGGCCGCGCTGTCGAAGTAAGCCGCGTGATCCGGCGCGCGGCAGCGTGCGCCGGGCGTCGTATCGTC
CTCTTTCGCTCGATCCCACG

Downstream 100 bases:

>100_bases
GCAGCCGGGGCGCCAGGAGAGAGGCTCCCCAGCGTGCTATAATATAAGGTTTCCAGCGGTTTGTTTTCTAAGCAGAGGCG
AGATTCGCGATGGCAAAGAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAIGFLMEHAGLTFP
EAVQELAQSVGLTVPHEPSTRGGGGAGGDYPAPVSKSVATALSDVMATACDYYRKQLRGAPAAIQYLKNRGLTGEIAARF
GLGYAPDGWQNLEAAFPNYRDDALVESGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGQVIGFGGRVLGSGEPKYLN
SPETPLFNKGSELYGLFEARLAIRERKYVLVVEGYMDVVALAQLGFPNAVATLGTACTPIHVQKLLRQTDTVIFSFDGDS
AGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYVREFGADAFSDQVERAMPLSQFLLNEAIAGKALDQPEGRAKAL
FDAKPLLQALPANALRAQIMHMIADRLDIPFEEVAALSEVDARIAAAPRQAPARSDRRRVTESEKRALRTLVMHPRIALA
LDDEQVATLRALPRIGELFAEVVDHARALGDGAEFRLLSDVLRTSPNSATYEEIFREILDYDENVRDLLLQNPDDEAVAE
RQREQERIAGEELQAAVLKMRYDACCDRLDRLARQSAFTPEELAELKHLNEQRTDMKRRLGL

Sequences:

>Translated_622_residues
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAIGFLMEHAGLTFP
EAVQELAQSVGLTVPHEPSTRGGGGAGGDYPAPVSKSVATALSDVMATACDYYRKQLRGAPAAIQYLKNRGLTGEIAARF
GLGYAPDGWQNLEAAFPNYRDDALVESGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGQVIGFGGRVLGSGEPKYLN
SPETPLFNKGSELYGLFEARLAIRERKYVLVVEGYMDVVALAQLGFPNAVATLGTACTPIHVQKLLRQTDTVIFSFDGDS
AGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYVREFGADAFSDQVERAMPLSQFLLNEAIAGKALDQPEGRAKAL
FDAKPLLQALPANALRAQIMHMIADRLDIPFEEVAALSEVDARIAAAPRQAPARSDRRRVTESEKRALRTLVMHPRIALA
LDDEQVATLRALPRIGELFAEVVDHARALGDGAEFRLLSDVLRTSPNSATYEEIFREILDYDENVRDLLLQNPDDEAVAE
RQREQERIAGEELQAAVLKMRYDACCDRLDRLARQSAFTPEELAELKHLNEQRTDMKRRLGL
>Mature_622_residues
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAIGFLMEHAGLTFP
EAVQELAQSVGLTVPHEPSTRGGGGAGGDYPAPVSKSVATALSDVMATACDYYRKQLRGAPAAIQYLKNRGLTGEIAARF
GLGYAPDGWQNLEAAFPNYRDDALVESGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGQVIGFGGRVLGSGEPKYLN
SPETPLFNKGSELYGLFEARLAIRERKYVLVVEGYMDVVALAQLGFPNAVATLGTACTPIHVQKLLRQTDTVIFSFDGDS
AGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYVREFGADAFSDQVERAMPLSQFLLNEAIAGKALDQPEGRAKAL
FDAKPLLQALPANALRAQIMHMIADRLDIPFEEVAALSEVDARIAAAPRQAPARSDRRRVTESEKRALRTLVMHPRIALA
LDDEQVATLRALPRIGELFAEVVDHARALGDGAEFRLLSDVLRTSPNSATYEEIFREILDYDENVRDLLLQNPDDEAVAE
RQREQERIAGEELQAAVLKMRYDACCDRLDRLARQSAFTPEELAELKHLNEQRTDMKRRLGL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=547, Percent_Identity=43.6928702010969, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68763; Mature: 68763

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGTAIGFLMEHAGLTFPEAVQELAQSVGLTVPHEPSTRGGGGAGGDYPAPVSKSVAT
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ALSDVMATACDYYRKQLRGAPAAIQYLKNRGLTGEIAARFGLGYAPDGWQNLEAAFPNYR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCC
DDALVESGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGQVIGFGGRVLGSGEPKYLN
CHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCC
SPETPLFNKGSELYGLFEARLAIRERKYVLVVEGYMDVVALAQLGFPNAVATLGTACTPI
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCHHHHHHCCCCCHH
HVQKLLRQTDTVIFSFDGDSAGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYVRE
HHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHH
FGADAFSDQVERAMPLSQFLLNEAIAGKALDQPEGRAKALFDAKPLLQALPANALRAQIM
HCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
HMIADRLDIPFEEVAALSEVDARIAAAPRQAPARSDRRRVTESEKRALRTLVMHPRIALA
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
LDDEQVATLRALPRIGELFAEVVDHARALGDGAEFRLLSDVLRTSPNSATYEEIFREILD
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHC
YDENVRDLLLQNPDDEAVAERQREQERIAGEELQAAVLKMRYDACCDRLDRLARQSAFTP
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EELAELKHLNEQRTDMKRRLGL
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGTAIGFLMEHAGLTFPEAVQELAQSVGLTVPHEPSTRGGGGAGGDYPAPVSKSVAT
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ALSDVMATACDYYRKQLRGAPAAIQYLKNRGLTGEIAARFGLGYAPDGWQNLEAAFPNYR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCC
DDALVESGLVIVSEKTDAQGAARRYDRFRERIMFPIRNVKGQVIGFGGRVLGSGEPKYLN
CHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCC
SPETPLFNKGSELYGLFEARLAIRERKYVLVVEGYMDVVALAQLGFPNAVATLGTACTPI
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCHHHHHHCCCCCHH
HVQKLLRQTDTVIFSFDGDSAGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYVRE
HHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHH
FGADAFSDQVERAMPLSQFLLNEAIAGKALDQPEGRAKALFDAKPLLQALPANALRAQIM
HCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
HMIADRLDIPFEEVAALSEVDARIAAAPRQAPARSDRRRVTESEKRALRTLVMHPRIALA
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
LDDEQVATLRALPRIGELFAEVVDHARALGDGAEFRLLSDVLRTSPNSATYEEIFREILD
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHC
YDENVRDLLLQNPDDEAVAERQREQERIAGEELQAAVLKMRYDACCDRLDRLARQSAFTP
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EELAELKHLNEQRTDMKRRLGL
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]