Click here to switch to the map view.

The map label for this gene is citH [H]

Identifier: 189352315

GI number: 189352315

Start: 365222

End: 366517

Strand: Reverse

Name: citH [H]

Synonym: BMULJ_03534

Alternate gene names: 189352315

Gene position: 366517-365222 (Counterclockwise)

Preceding gene: 189352318

Following gene: 189352314

Centisome position: 14.82

GC content: 66.9

Gene sequence:

>1296_bases
ATGCACCCCTCGTCCAGTCTCTCGACCGGGCGCTCGAAGGCCGCTGCGGTCTTCCGCGTCACGGCCGGGAATTTTCTCGA
GCAGTTCGACTTCTTTCTGTTCGGCTTCTACGCCACGCAGATCGCCAACGTGTTCTTTCCCGCCGCCAGCGAGTTCGCGT
CACTGATGATGACGTTCGCCGTGTTCGGCGCCGGCTTCCTGATGCGGCCGCTCGGCGCGATCGTGCTCGGCGCGTACATC
GACGACGTCGGCCGTCGCAAGGGCCTGATCGTCACGCTGTCGATCATGGCGAGCGGCACGATCCTGATCGCGTTCGTGCC
CGGCTACGCGACCATCGGCCTGCTCGCACCCGCGCTCGTGCTGCTCGGGCGGCTGCTGCAGGGCTTCTCGGCCGGCGCGG
AGCTTGGCGGCGTATCCGTCTATCTGGCCGAAATGGCTACGCCCGGCCGCAAGGGCTTCTTCACGAGCTGGCAGTCCGCG
AGCCAGCAGGTCGCGATCGTCGTCGCGGCGGCGCTCGGCTTCGCGCTGAATCGCTGGCTGAGCGCGGCCGACATCGCCGC
GTGGGGCTGGCGCGTGCCGTTCTTCGTCGGCTGCATGATCGTGCCGTTCATCTTCATGCTGCGTCGCAAGCTCGAGGAAA
CGCAGGAATTCACCGCGCGCCGTCATCGTCCGACGATGAAGGAAGTATTCGCCACGCTGGCTCAGAACTGGGCCGTCGTC
GTGGCCGGCATGATGCTGGTCGCGATGACCACCGCGAGTTTCTACCTGATCACCGTGTATGCGCCGACGTTCGGCAAGAC
CGTCCTGCATCTGAGCACCGCGGACAGCCTGCTCGTGACGCTGTGCGTCGGCGTCTCGAACTTCGTGTGGCTGCCGATCG
GCGGTGCGCTGTCCGACCGCATCGGCCGCCGCCCGCTGCTCGTCGCGATGACGGTGCTCGCGATCGCGACCGCCTATCCG
GCGCTGTCGCTGCTCGCCCATGCGCCGACGTTCATGAACATGCTGCTCGTGCTGCTGTGGCTGTCGTTCATGTACGGCCT
CTACAACGGCGCGATGATCGTCGCGCTCACCGAAGTGATGCCCGTGCAGGTGCGCGTGGCCGGATTCTCGCTCGCGTACA
GCCTCGCGACGGCCGTGTTCGGCGGCTTCACGCCGGCGATCTCGACCGCGCTGATCCACATGACGGGCGACAAGGCCGCG
CCCGGCTATTGGATGAGCTTCGCCGCCGTATGTGCGCTGCTCGCGACGTTCGCGCTGTATCGCCGCCAGGCCGCGACGCT
GACGCCCGCGCATTGA

Upstream 100 bases:

>100_bases
CGTTCAGAAAATTGCACTTATCGTTTCTGCGGCATCGCGCGAACATTCGCGAAAACAACAATCGCCGCCCTCCCTCCCCG
CATTTTCCGGAGACATCCCG

Downstream 100 bases:

>100_bases
CGGTCCGCGCGTCCCCCATGCTCACGTAACCTTCCCGTTCGCACGACCACGACGATGAAAAATCTGCTGCTGAAACTTTG
CGCGACCGCGCTCGTCGCGA

Product: permease of the major facilitator superfamily

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MHPSSSLSTGRSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPAASEFASLMMTFAVFGAGFLMRPLGAIVLGAYI
DDVGRRKGLIVTLSIMASGTILIAFVPGYATIGLLAPALVLLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSA
SQQVAIVVAAALGFALNRWLSAADIAAWGWRVPFFVGCMIVPFIFMLRRKLEETQEFTARRHRPTMKEVFATLAQNWAVV
VAGMMLVAMTTASFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDRIGRRPLLVAMTVLAIATAYP
ALSLLAHAPTFMNMLLVLLWLSFMYGLYNGAMIVALTEVMPVQVRVAGFSLAYSLATAVFGGFTPAISTALIHMTGDKAA
PGYWMSFAAVCALLATFALYRRQAATLTPAH

Sequences:

>Translated_431_residues
MHPSSSLSTGRSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPAASEFASLMMTFAVFGAGFLMRPLGAIVLGAYI
DDVGRRKGLIVTLSIMASGTILIAFVPGYATIGLLAPALVLLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSA
SQQVAIVVAAALGFALNRWLSAADIAAWGWRVPFFVGCMIVPFIFMLRRKLEETQEFTARRHRPTMKEVFATLAQNWAVV
VAGMMLVAMTTASFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDRIGRRPLLVAMTVLAIATAYP
ALSLLAHAPTFMNMLLVLLWLSFMYGLYNGAMIVALTEVMPVQVRVAGFSLAYSLATAVFGGFTPAISTALIHMTGDKAA
PGYWMSFAAVCALLATFALYRRQAATLTPAH
>Mature_431_residues
MHPSSSLSTGRSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPAASEFASLMMTFAVFGAGFLMRPLGAIVLGAYI
DDVGRRKGLIVTLSIMASGTILIAFVPGYATIGLLAPALVLLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSA
SQQVAIVVAAALGFALNRWLSAADIAAWGWRVPFFVGCMIVPFIFMLRRKLEETQEFTARRHRPTMKEVFATLAQNWAVV
VAGMMLVAMTTASFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDRIGRRPLLVAMTVLAIATAYP
ALSLLAHAPTFMNMLLVLLWLSFMYGLYNGAMIVALTEVMPVQVRVAGFSLAYSLATAVFGGFTPAISTALIHMTGDKAA
PGYWMSFAAVCALLATFALYRRQAATLTPAH

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=412, Percent_Identity=29.6116504854369, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1788942, Length=427, Percent_Identity=33.2552693208431, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788292, Length=392, Percent_Identity=28.8265306122449, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789941, Length=376, Percent_Identity=30.0531914893617, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87082231, Length=199, Percent_Identity=26.1306532663317, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46228; Mature: 46228

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPSSSLSTGRSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPAASEFASLMMTFA
CCCCCCCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VFGAGFLMRPLGAIVLGAYIDDVGRRKGLIVTLSIMASGTILIAFVPGYATIGLLAPALV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHH
LLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSASQQVAIVVAAALGFALNRWL
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
SAADIAAWGWRVPFFVGCMIVPFIFMLRRKLEETQEFTARRHRPTMKEVFATLAQNWAVV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VAGMMLVAMTTASFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDR
HHHHHHHHHHHHHEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCEEEEECCCHHHHH
IGRRPLLVAMTVLAIATAYPALSLLAHAPTFMNMLLVLLWLSFMYGLYNGAMIVALTEVM
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHC
PVQVRVAGFSLAYSLATAVFGGFTPAISTALIHMTGDKAAPGYWMSFAAVCALLATFALY
EEEEEEEHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RRQAATLTPAH
HHHHCCCCCCC
>Mature Secondary Structure
MHPSSSLSTGRSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPAASEFASLMMTFA
CCCCCCCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VFGAGFLMRPLGAIVLGAYIDDVGRRKGLIVTLSIMASGTILIAFVPGYATIGLLAPALV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHH
LLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSASQQVAIVVAAALGFALNRWL
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
SAADIAAWGWRVPFFVGCMIVPFIFMLRRKLEETQEFTARRHRPTMKEVFATLAQNWAVV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VAGMMLVAMTTASFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDR
HHHHHHHHHHHHHEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCEEEEECCCHHHHH
IGRRPLLVAMTVLAIATAYPALSLLAHAPTFMNMLLVLLWLSFMYGLYNGAMIVALTEVM
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHC
PVQVRVAGFSLAYSLATAVFGGFTPAISTALIHMTGDKAAPGYWMSFAAVCALLATFALY
EEEEEEEHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RRQAATLTPAH
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]