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The map label for this gene is rhtB [H]

Identifier: 189352299

GI number: 189352299

Start: 347384

End: 348016

Strand: Reverse

Name: rhtB [H]

Synonym: BMULJ_03518

Alternate gene names: 189352299

Gene position: 348016-347384 (Counterclockwise)

Preceding gene: 189352300

Following gene: 189352289

Centisome position: 14.07

GC content: 66.51

Gene sequence:

>633_bases
GTGCCGCCGATGTCCACCCTGCTCGCTTTCGGACTCGTGTCGCTCGGCATGGTCCTGACGCCCGGGCCGAACATGATCTA
CTTGATCTCGCGGTCGATCTGTCAGGGTCGACGTGCAGGCCTGGTGTCGCTCGGCGGCGTGGCGCTCGGGTTCGTGTTCT
ACATGTTTTGCGCGGCGTTCGGCATCGCCGCGCTCGTGCTGACCGTGCCTTACGCGTACGACGCATTGCGCGTCGCCGGC
GCGCTATATCTCGCGTATCTCGCCTGGCAGGCGCTCAAGCCCGGCGGACGCTCGGCGTTCCAGGTCCGGCCGTTGTCGCC
CGATAGCCGCCGGAAACTGTTCACGATGGGCTTCGTCACGAATCTCACGAATCCGAAGATCGCGGTGATGTATCTGTCGC
TGCTGCCGCAGTTCATTTCACCGGAACATGGCAGCGTCCTCGCGCAATCGCTCGCGCTCGGGTGCGTGCAAATCGCGGTG
AGCGTCAGCGTCAATGCGCTGATCGCGAGCATGGCGGGGTCCATCGCCGGATTTCTCGCGCGCCGGCCGGCCTGGGCACT
GGCGCAGCGCTGGCTGATGGGCACGGTGCTGGCCGGGCTCGCGGCACGCATCGCGCTCGAATCGCGCCGCTAG

Upstream 100 bases:

>100_bases
CATGCGCGGCTTGCAGATTATTCACGCCGGATGCCCGCGATATGCAATACATTGCACGGAGCCTGTCGACCCAACTTTCC
CCGCACCGAAGGAGACCGCC

Downstream 100 bases:

>100_bases
GAAGACGCCCGCTGCCATACGCCGTGATGTAAAACGGCCGCCGTGATGCCACGGCGGCCGCTCTCCGGAAACGCGCGACG
TTACGCCGTCGCGCGCCGCT

Product: putative threonine efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MPPMSTLLAFGLVSLGMVLTPGPNMIYLISRSICQGRRAGLVSLGGVALGFVFYMFCAAFGIAALVLTVPYAYDALRVAG
ALYLAYLAWQALKPGGRSAFQVRPLSPDSRRKLFTMGFVTNLTNPKIAVMYLSLLPQFISPEHGSVLAQSLALGCVQIAV
SVSVNALIASMAGSIAGFLARRPAWALAQRWLMGTVLAGLAARIALESRR

Sequences:

>Translated_210_residues
MPPMSTLLAFGLVSLGMVLTPGPNMIYLISRSICQGRRAGLVSLGGVALGFVFYMFCAAFGIAALVLTVPYAYDALRVAG
ALYLAYLAWQALKPGGRSAFQVRPLSPDSRRKLFTMGFVTNLTNPKIAVMYLSLLPQFISPEHGSVLAQSLALGCVQIAV
SVSVNALIASMAGSIAGFLARRPAWALAQRWLMGTVLAGLAARIALESRR
>Mature_209_residues
PPMSTLLAFGLVSLGMVLTPGPNMIYLISRSICQGRRAGLVSLGGVALGFVFYMFCAAFGIAALVLTVPYAYDALRVAGA
LYLAYLAWQALKPGGRSAFQVRPLSPDSRRKLFTMGFVTNLTNPKIAVMYLSLLPQFISPEHGSVLAQSLALGCVQIAVS
VSVNALIASMAGSIAGFLARRPAWALAQRWLMGTVLAGLAARIALESRR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=170, Percent_Identity=33.5294117647059, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1788099, Length=199, Percent_Identity=29.1457286432161, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786522, Length=127, Percent_Identity=29.9212598425197, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1788932, Length=184, Percent_Identity=30.4347826086957, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22310; Mature: 22179

Theoretical pI: Translated: 11.13; Mature: 11.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPMSTLLAFGLVSLGMVLTPGPNMIYLISRSICQGRRAGLVSLGGVALGFVFYMFCAAF
CCCHHHHHHHHHHHHCEEECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GIAALVLTVPYAYDALRVAGALYLAYLAWQALKPGGRSAFQVRPLSPDSRRKLFTMGFVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHH
NLTNPKIAVMYLSLLPQFISPEHGSVLAQSLALGCVQIAVSVSVNALIASMAGSIAGFLA
HCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRPAWALAQRWLMGTVLAGLAARIALESRR
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PPMSTLLAFGLVSLGMVLTPGPNMIYLISRSICQGRRAGLVSLGGVALGFVFYMFCAAF
CCHHHHHHHHHHHHCEEECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GIAALVLTVPYAYDALRVAGALYLAYLAWQALKPGGRSAFQVRPLSPDSRRKLFTMGFVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHH
NLTNPKIAVMYLSLLPQFISPEHGSVLAQSLALGCVQIAVSVSVNALIASMAGSIAGFLA
HCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRPAWALAQRWLMGTVLAGLAARIALESRR
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]