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The map label for this gene is shiA [H]

Identifier: 189352233

GI number: 189352233

Start: 273641

End: 274978

Strand: Reverse

Name: shiA [H]

Synonym: BMULJ_03451

Alternate gene names: 189352233

Gene position: 274978-273641 (Counterclockwise)

Preceding gene: 189352234

Following gene: 189352230

Centisome position: 11.12

GC content: 70.25

Gene sequence:

>1338_bases
ATGTATCCAACCGACACCGTGCAGTCCCCGAGCGCCGCCCGGCCGCTCGCCGACCGGCAGCTTCGCCGGATCGTCATCGC
CTCCGTCGCCGGCAACGCGATGGAGTGGTATGACTTCTTCGTGTACGGCACCGCCGCGGCGCTCGTGTTCGGCCACGTGT
TCTTTCCGCCCGGCGCGTCGCCGCTCGCCGGCAGCCTCGCCGCGTTCGCGGCGTTCGCGCTCGGCTTCGTCGCGCGGCCG
CTCGGCGGCATCGTGTTCGGCCATGTCGGCGACCGCTACGGCCGCAAGGCGTCGCTCGTCTGGACGCTGCTGATCATGGG
CGCGTCGACGTTCGCGATCGGGCTGCTGCCGACCTACGCGCAGGCCGGCATCTGGGCGCCGGCCGCGCTCGTCGTGCTGC
GCCTGCTGCAAGGCATCGCCTCCGGCGGCGAATGGGGCGGCGGCGTGCTGATCATCAGCGAGAACGCGCCGCCCGAACAG
CGCGGCTACTACGCGGCCTGGAGCCAGCTCGGCGTCGGCGGCGGGTTCGTGCTGTCGTCCGCCGCGTTTCTCGCCGCGCA
GACGCTGCCGAACGACATGTTCATGACGTGGGGCTGGCGGCTGCCGTTCCTCGCGAGCATCGTGATCTTCGCGATCGGCA
TCTACATCCGCCGCCATCTGCCGGAAAGTCGCGACTACGAGCAGGCCGGCAAGCGCGGCGCGCATACGCATCTGCCGATC
GTCGAGTGCATCCGCCGTCATCCGAAGGAAATCCTGCTCGCGATGGGGCTGCGCGTGGCCGAGAACGGCGGCGCGTATAT
CTTCCTCGCGTTCTCGCTCGTCTACGGCAAATACGTCGGCATCCCGAACGGCGTGATGCTGACCGGCGTGATGATCGCGA
TGATCGTCGAGATGGGCGCGATGCTCGCGTGGGGCCGGCTGTCCGACCGGATCGGCCGCAAGCCCGTCTACCTGATCGGC
GCGCTCGGCCTCGTCGCCTGCGCGTTTCCGTTCTTCTGGCTGCTCGACACGCGCGTGACGCCGCTCGTCTGGCTCGCGCT
GACGGTCTGCACGGCCGTCAGCCACGGCGCGATGATCGGCACGCTGCCGGCGCTCGTCGGCGAGCTGTTCAGCACCGAAG
TGCGCTACTCGGGCGTCGCGCTCGGCCATGAAGTCGCGTCGATCTTCGCGGGCGGGATGTCGCCGCTGATCGCGACCGCC
TTGCTGCAGCGCTATCAGGCGTCCTGGCCCGTCTCGCTGTTTCTCGTCGCGCTCGGGCTCGTGACCGTCGCGACGCTGTG
CGCGATGCGCGAGACGCGCATCGTGCGCGCCGGGGGCGCGCGCGGCGGCGACGCATGA

Upstream 100 bases:

>100_bases
TTTGCGGATTTGACCGGAGCGGCAGCGCCGACAACACTCGTCGCGAGTGCCGCACCGGTTCCGGGACGGCACGCGTTCGC
GTTCCCCACCCGAGTCCGCC

Downstream 100 bases:

>100_bases
TGGTGCGGTCGTACGTCGCGCGCCGCGGCGGCTGCGTCGGTCGTGCGGTTAGCCGATCATGCGCTCGGGCGACGCCGCGC
GCCGTCGAACGTCGGTGCGA

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MYPTDTVQSPSAARPLADRQLRRIVIASVAGNAMEWYDFFVYGTAAALVFGHVFFPPGASPLAGSLAAFAAFALGFVARP
LGGIVFGHVGDRYGRKASLVWTLLIMGASTFAIGLLPTYAQAGIWAPAALVVLRLLQGIASGGEWGGGVLIISENAPPEQ
RGYYAAWSQLGVGGGFVLSSAAFLAAQTLPNDMFMTWGWRLPFLASIVIFAIGIYIRRHLPESRDYEQAGKRGAHTHLPI
VECIRRHPKEILLAMGLRVAENGGAYIFLAFSLVYGKYVGIPNGVMLTGVMIAMIVEMGAMLAWGRLSDRIGRKPVYLIG
ALGLVACAFPFFWLLDTRVTPLVWLALTVCTAVSHGAMIGTLPALVGELFSTEVRYSGVALGHEVASIFAGGMSPLIATA
LLQRYQASWPVSLFLVALGLVTVATLCAMRETRIVRAGGARGGDA

Sequences:

>Translated_445_residues
MYPTDTVQSPSAARPLADRQLRRIVIASVAGNAMEWYDFFVYGTAAALVFGHVFFPPGASPLAGSLAAFAAFALGFVARP
LGGIVFGHVGDRYGRKASLVWTLLIMGASTFAIGLLPTYAQAGIWAPAALVVLRLLQGIASGGEWGGGVLIISENAPPEQ
RGYYAAWSQLGVGGGFVLSSAAFLAAQTLPNDMFMTWGWRLPFLASIVIFAIGIYIRRHLPESRDYEQAGKRGAHTHLPI
VECIRRHPKEILLAMGLRVAENGGAYIFLAFSLVYGKYVGIPNGVMLTGVMIAMIVEMGAMLAWGRLSDRIGRKPVYLIG
ALGLVACAFPFFWLLDTRVTPLVWLALTVCTAVSHGAMIGTLPALVGELFSTEVRYSGVALGHEVASIFAGGMSPLIATA
LLQRYQASWPVSLFLVALGLVTVATLCAMRETRIVRAGGARGGDA
>Mature_445_residues
MYPTDTVQSPSAARPLADRQLRRIVIASVAGNAMEWYDFFVYGTAAALVFGHVFFPPGASPLAGSLAAFAAFALGFVARP
LGGIVFGHVGDRYGRKASLVWTLLIMGASTFAIGLLPTYAQAGIWAPAALVVLRLLQGIASGGEWGGGVLIISENAPPEQ
RGYYAAWSQLGVGGGFVLSSAAFLAAQTLPNDMFMTWGWRLPFLASIVIFAIGIYIRRHLPESRDYEQAGKRGAHTHLPI
VECIRRHPKEILLAMGLRVAENGGAYIFLAFSLVYGKYVGIPNGVMLTGVMIAMIVEMGAMLAWGRLSDRIGRKPVYLIG
ALGLVACAFPFFWLLDTRVTPLVWLALTVCTAVSHGAMIGTLPALVGELFSTEVRYSGVALGHEVASIFAGGMSPLIATA
LLQRYQASWPVSLFLVALGLVTVATLCAMRETRIVRAGGARGGDA

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=425, Percent_Identity=37.8823529411765, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1790550, Length=434, Percent_Identity=35.2534562211982, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1789941, Length=437, Percent_Identity=38.4439359267735, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1788942, Length=409, Percent_Identity=29.3398533007335, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87082404, Length=344, Percent_Identity=23.8372093023256, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082231, Length=434, Percent_Identity=23.2718894009217, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47379; Mature: 47379

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHEEECCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]