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The map label for this gene is prpB [C]

Identifier: 189352186

GI number: 189352186

Start: 222282

End: 223043

Strand: Reverse

Name: prpB [C]

Synonym: BMULJ_03404

Alternate gene names: 189352186

Gene position: 223043-222282 (Counterclockwise)

Preceding gene: 189352187

Following gene: 189352184

Centisome position: 9.02

GC content: 69.82

Gene sequence:

>762_bases
ATGACAACTGCTGACAAGCATGCCCGCTTTCGCGCACTGCATCGCGCCGGCGATCCGCTGGTGCTGTTCAACGTCTGGGA
TGCGGGCAGCGCGCGCGCCGTCGCCGACACCGGCGCGCCCGCCCTCGCGACCGGAAGCTGGTCGATCGCGAAAGCCAACG
GTTACGCGGACGGCGAAGACATGCCGCGCGCGCTGGCCATCGAGCTGCTTGCCCGCATCGTCGCCGCGACCGACCTGCCG
GTCAGCGCCGATCTCGAAAGCGGCTATGGCGAGCAACCCGACGACGTCGCCGATACGATCGCGCTCAGCATCGAGGCCGG
CGCGATCGGCTGCAATCTCGAGGACAGCGTTCCCGCGACGGGCGAAGTGCGCAGCGTCGACGATGCGGTTGCGCGCATCG
CCGCAGCGCGCCGCGCGGCCGACAAGAACGGCGGCGGATATTTCATCAATGCGCGCACGGACGTCTGTTTCGTCGTCCCC
GCCGACACGCACGACGAGGCGCTGATCGACGCGGTGCTCGTGCGCGCACGCGCCTACGCGGAAGCCGGTGCGGACGGGCT
GTTCGTGCCGGGCCTGAAGTCGCCCGCGCTGATCCGCAAGCTGACGGCGGCATCGCCGCTGCCGGTGAACGTGATGCGCG
TGGCCGACACGCCGACGATTGCGGAACTCGCCGCATGCGGCGTGTCGCGCATCAGCCACGGGCCGTATCCGTACATTCAG
GCGATGAAGGCGCTTGCGGCGCTCGTGCCGCAGCGCGGCTGA

Upstream 100 bases:

>100_bases
GCCCGCGCGCGACTGAGCCGTCGCGAATTTTCCGTGCGCGCGCGGCGCGAAAGCGTATCCTTCATCGCGGCACTCGATCC
GCCAACCAGGAGACACGCAT

Downstream 100 bases:

>100_bases
GGCTGGGCCCGAGGCTGACGTTGATGCTGACGCCGACGCCGCGCGCGTCGGCGCGATCGCATCGAAGCGGAACCGCGCGG
CACGCGCGTGTTGCTGCGCG

Product: putative PEP phosphonomutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTTADKHARFRALHRAGDPLVLFNVWDAGSARAVADTGAPALATGSWSIAKANGYADGEDMPRALAIELLARIVAATDLP
VSADLESGYGEQPDDVADTIALSIEAGAIGCNLEDSVPATGEVRSVDDAVARIAAARRAADKNGGGYFINARTDVCFVVP
ADTHDEALIDAVLVRARAYAEAGADGLFVPGLKSPALIRKLTAASPLPVNVMRVADTPTIAELAACGVSRISHGPYPYIQ
AMKALAALVPQRG

Sequences:

>Translated_253_residues
MTTADKHARFRALHRAGDPLVLFNVWDAGSARAVADTGAPALATGSWSIAKANGYADGEDMPRALAIELLARIVAATDLP
VSADLESGYGEQPDDVADTIALSIEAGAIGCNLEDSVPATGEVRSVDDAVARIAAARRAADKNGGGYFINARTDVCFVVP
ADTHDEALIDAVLVRARAYAEAGADGLFVPGLKSPALIRKLTAASPLPVNVMRVADTPTIAELAACGVSRISHGPYPYIQ
AMKALAALVPQRG
>Mature_252_residues
TTADKHARFRALHRAGDPLVLFNVWDAGSARAVADTGAPALATGSWSIAKANGYADGEDMPRALAIELLARIVAATDLPV
SADLESGYGEQPDDVADTIALSIEAGAIGCNLEDSVPATGEVRSVDDAVARIAAARRAADKNGGGYFINARTDVCFVVPA
DTHDEALIDAVLVRARAYAEAGADGLFVPGLKSPALIRKLTAASPLPVNVMRVADTPTIAELAACGVSRISHGPYPYIQA
MKALAALVPQRG

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=171, Percent_Identity=32.7485380116959, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 26171; Mature: 26040

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTADKHARFRALHRAGDPLVLFNVWDAGSARAVADTGAPALATGSWSIAKANGYADGED
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCEEECCCEEEEECCCCCCCCC
MPRALAIELLARIVAATDLPVSADLESGYGEQPDDVADTIALSIEAGAIGCNLEDSVPAT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHEEEEEECCEEECCCCCCCCCC
GEVRSVDDAVARIAAARRAADKNGGGYFINARTDVCFVVPADTHDEALIDAVLVRARAYA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
EAGADGLFVPGLKSPALIRKLTAASPLPVNVMRVADTPTIAELAACGVSRISHGPYPYIQ
HCCCCCEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHH
AMKALAALVPQRG
HHHHHHHHCCCCC
>Mature Secondary Structure 
TTADKHARFRALHRAGDPLVLFNVWDAGSARAVADTGAPALATGSWSIAKANGYADGED
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCEEECCCEEEEECCCCCCCCC
MPRALAIELLARIVAATDLPVSADLESGYGEQPDDVADTIALSIEAGAIGCNLEDSVPAT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHEEEEEECCEEECCCCCCCCCC
GEVRSVDDAVARIAAARRAADKNGGGYFINARTDVCFVVPADTHDEALIDAVLVRARAYA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
EAGADGLFVPGLKSPALIRKLTAASPLPVNVMRVADTPTIAELAACGVSRISHGPYPYIQ
HCCCCCEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHH
AMKALAALVPQRG
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]