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The map label for this gene is purR [H]

Identifier: 189352135

GI number: 189352135

Start: 161927

End: 162919

Strand: Reverse

Name: purR [H]

Synonym: BMULJ_03353

Alternate gene names: 189352135

Gene position: 162919-161927 (Counterclockwise)

Preceding gene: 189352137

Following gene: 189352133

Centisome position: 6.59

GC content: 68.08

Gene sequence:

>993_bases
ATGAAAAAAGTTTCCCCGACGATCCGGGACGTCGCCGCGGAAGCCGGCGTATCGGTCGCGACGGTGTCGAAGTATGTGAA
CGGCACGCAGCGCTTTTCGCCGAGCGTCGAGGCGCGGCTCAAGGACGCGATCGAGCGGCTCGGCTATCGGTCGAATCCGC
TCGCGCGCTCGATGATCACCGGCCGCACGCGCACGATCGGCCTCGTCATCCTCGACATCAGCAACCCGCACTTCACGAAC
GTCGTGAAAGGCGCGAACCGCGTCGCGCTGCAGCACGACTACACGCTGCTGCTCGTCGATACCGAGGAGAATCAGGCGCG
CGAACGCTCGCTGATCGAGGCGCTCGCGCAGCGCGTCGACGGTCTGATCGTCAGCACGCGGATGCCCGACGAAGAAGCGG
GCTGGATGCTCGATTTGCACAAGCCGCTCGTGCTGCTGCGGCGCAGCCCGGGCCTGCCGATTCCGAGCGTCGGCATCGAC
AACCGGCTGTCGACGTACATGCTCGCGCGCCATCTGCTGAACCTCGGGCACACGCGTATCGCCTATCTCGGCTTCGGGCC
CGCGCGCGTGAACGACGAGCGGATCGAAGGCGCGCGCGACTGTCTCGCGGAAGCCGGCCTGACGCTCGACGTCTACGATG
CGCACGCGCCGACCGCCGAGGCCGGCGAGCGCGCGTGCTCGCGCGTGATGCTCGGGCCGCAGCGCCCGCAGGCCGTGATC
TGCTACAACGACCTGATCGCGCTCGGCTTCATGAAGGAAGCGGCGTCGCTCGGTTTCCGGCTGCCGCAGGACGTGTCCGT
CACCGGCGTCGACAACGTGCCGTACGGCGAATACGCGGCGCCCGCGCTGACCACCGTCGACATCCAGAGCGAGAACATGG
GCGAGCTCGCGATGCAAAAGCTGATCGACGCGCTCGCGGGCCGCACCGACGCGAGCTACTCGACGTTCGAGCCGCGGCTG
ATCGTGCGCGCATCGACGGCGCCGGCCGCGTGA

Upstream 100 bases:

>100_bases
CTCGAGCGGCATCCGCATTTTCCCGAGCGGGCGCGGCGTGCCTTGCACGGACTTTTTCTGCCACAATCCGGCGTTACCGA
AAGAAAACGTTACCCTTCGA

Downstream 100 bases:

>100_bases
AGCAGCGCGTGCGCCGCCCGTCGCGGCGCACACGCGCGAACGACTGCCGTCATGCATCGAGCTTCGCCGGCTTCATCTTC
GGCGTCAGCGCATGCATGAT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKKVSPTIRDVAAEAGVSVATVSKYVNGTQRFSPSVEARLKDAIERLGYRSNPLARSMITGRTRTIGLVILDISNPHFTN
VVKGANRVALQHDYTLLLVDTEENQARERSLIEALAQRVDGLIVSTRMPDEEAGWMLDLHKPLVLLRRSPGLPIPSVGID
NRLSTYMLARHLLNLGHTRIAYLGFGPARVNDERIEGARDCLAEAGLTLDVYDAHAPTAEAGERACSRVMLGPQRPQAVI
CYNDLIALGFMKEAASLGFRLPQDVSVTGVDNVPYGEYAAPALTTVDIQSENMGELAMQKLIDALAGRTDASYSTFEPRL
IVRASTAPAA

Sequences:

>Translated_330_residues
MKKVSPTIRDVAAEAGVSVATVSKYVNGTQRFSPSVEARLKDAIERLGYRSNPLARSMITGRTRTIGLVILDISNPHFTN
VVKGANRVALQHDYTLLLVDTEENQARERSLIEALAQRVDGLIVSTRMPDEEAGWMLDLHKPLVLLRRSPGLPIPSVGID
NRLSTYMLARHLLNLGHTRIAYLGFGPARVNDERIEGARDCLAEAGLTLDVYDAHAPTAEAGERACSRVMLGPQRPQAVI
CYNDLIALGFMKEAASLGFRLPQDVSVTGVDNVPYGEYAAPALTTVDIQSENMGELAMQKLIDALAGRTDASYSTFEPRL
IVRASTAPAA
>Mature_330_residues
MKKVSPTIRDVAAEAGVSVATVSKYVNGTQRFSPSVEARLKDAIERLGYRSNPLARSMITGRTRTIGLVILDISNPHFTN
VVKGANRVALQHDYTLLLVDTEENQARERSLIEALAQRVDGLIVSTRMPDEEAGWMLDLHKPLVLLRRSPGLPIPSVGID
NRLSTYMLARHLLNLGHTRIAYLGFGPARVNDERIEGARDCLAEAGLTLDVYDAHAPTAEAGERACSRVMLGPQRPQAVI
CYNDLIALGFMKEAASLGFRLPQDVSVTGVDNVPYGEYAAPALTTVDIQSENMGELAMQKLIDALAGRTDASYSTFEPRL
IVRASTAPAA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=30.3303303303303, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789202, Length=286, Percent_Identity=34.6153846153846, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=32.7327327327327, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=34.1463414634146, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=33.9805825242718, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1789068, Length=328, Percent_Identity=31.0975609756098, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=27.1293375394322, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787906, Length=263, Percent_Identity=30.4182509505703, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790715, Length=331, Percent_Identity=23.2628398791541, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=26.791277258567, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=26.2686567164179, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786268, Length=277, Percent_Identity=27.4368231046931, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789456, Length=308, Percent_Identity=23.7012987012987, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35878; Mature: 35878

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVSPTIRDVAAEAGVSVATVSKYVNGTQRFSPSVEARLKDAIERLGYRSNPLARSMIT
CCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
GRTRTIGLVILDISNPHFTNVVKGANRVALQHDYTLLLVDTEENQARERSLIEALAQRVD
CCCEEEEEEEEECCCCCHHHHHCCCCEEEEEECEEEEEEECCCHHHHHHHHHHHHHHHHC
GLIVSTRMPDEEAGWMLDLHKPLVLLRRSPGLPIPSVGIDNRLSTYMLARHLLNLGHTRI
CEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
AYLGFGPARVNDERIEGARDCLAEAGLTLDVYDAHAPTAEAGERACSRVMLGPQRPQAVI
EEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEE
CYNDLIALGFMKEAASLGFRLPQDVSVTGVDNVPYGEYAAPALTTVDIQSENMGELAMQK
EEHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
LIDALAGRTDASYSTFEPRLIVRASTAPAA
HHHHHHCCCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKVSPTIRDVAAEAGVSVATVSKYVNGTQRFSPSVEARLKDAIERLGYRSNPLARSMIT
CCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
GRTRTIGLVILDISNPHFTNVVKGANRVALQHDYTLLLVDTEENQARERSLIEALAQRVD
CCCEEEEEEEEECCCCCHHHHHCCCCEEEEEECEEEEEEECCCHHHHHHHHHHHHHHHHC
GLIVSTRMPDEEAGWMLDLHKPLVLLRRSPGLPIPSVGIDNRLSTYMLARHLLNLGHTRI
CEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
AYLGFGPARVNDERIEGARDCLAEAGLTLDVYDAHAPTAEAGERACSRVMLGPQRPQAVI
EEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEE
CYNDLIALGFMKEAASLGFRLPQDVSVTGVDNVPYGEYAAPALTTVDIQSENMGELAMQK
EEHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
LIDALAGRTDASYSTFEPRLIVRASTAPAA
HHHHHHCCCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA